Predicting folded structures of proteins

ABSTRACT

A method is presented for predicting the folded structure of proteins that comprises obtaining an alignment of the sequences of a set of homologous proteins, using patterns of conservation and variation of the sequence between proteins with clearly defined evolutionary relationships to assign positions in the alignment to the surface of the folded structure, the inside of the folded structure, active site, or parsing segments, assigning secondary structures by identifying periodicity in said assignments, and then assembling the secondary structural units into a globular form using distance constraints imposed by disulfide bridges, active site assignments, and covariation analysis.

CROSS REFERENCES TO RELATED APPLICATIONS

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STATEMENT OF RIGHTS TO INVENTIONS MADE UNDER FEDERALLY-SPONSORED RESEARCH

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BACKGROUND OF THE INVENTION

1. Field of the Invention

This invention relates to methods for constructing models for the secondary structure, supersecondary structure, and tertiary structure of proteins in the absence of any crystallographic information for any member of the protein family. More particularly, the present invention pertains to methods for extracting structural information from a set of aligned sequences of homologous (related by common ancestry) proteins for these purposes. More particularly, the present invention pertains to methods that extract structural information concerning a protein fold from the patterns of conservation and divergence of amino acid sequence within a set of homologous protein sequences, where this information is extracted using algorithms that reflect the evolutionary processes by which the protein family emerged.

2. Description of the Related Art

Proteins are linear polypeptide chains composed of 20 different amino acid building blocks. Determining the sequence of amino acids in a protein is now experimentally routine, both by direct chemical analysis of the proteins themselves, or by translation of a gene that encodes the protein. Existing data bases contain over 10 million amino acids.

The linear polypeptide sequence provides only a small part of the structural information that is important to the biochemist, however. The polypeptide chain folds to give secondary structural units (most commonly alpha helices and beta strands) which then fold to give supersecondary structures (for example, beta sheets) and a tertiary structure. Most of the behaviors of a protein are determined by its secondary and tertiary structure, including those that are important for allowing the protein to function in a living system. Further, the folded structure must be known before pharmaceuticals can be designed to bind to the protein.

The utility of methods able to predict secondary structure of a protein from sequence data alone is obvious to any individual ordinarily skilled in the art. High quality secondary structure predictions are useful for identifying antigenic sites on a protein molecule, as guides for site directed mutagenesis studies, and for understanding the interaction of a protein with other molecules. Further, high quality secondary structure predictions are prerequisites for building tertiary structural models for proteins.

The importance of methods for predicting the folded structure of proteins from sequence data has been appreciated by biochemists for over 30 years, and a corresponding three decades of labor have been devoted to efforts to develop such methods. The problem has proven to be extremely difficult to solve. In the process, a very large number of publications have appeared describing approaches towards solution to the structure prediction problem. Many of the classical approaches attempting to develop methods for predicting the folded structure of proteins from sequence data are summarized in a book G. Fasman, editor, Prediction of Protein Structure and the Principles of Protein Conformation, NY Plenum (1989)!, which is incorporated herein by reference.

It is clearly impossible to provide in this disclosure a complete description of the entire body of classical work in the field of protein structure prediction. What is presented is a summary of these classical methods, together with a brief comment on their efficacy, the prior art known to the Applicant that serves as the closest precedent for the method of the present invention, and criteria that allow the present invention to be distinguished from all aspects of the prior art known to the Applicant.

First, the method of the present invention is de novo, that is, it allows the prediction of the folded structure of a protein without the need for any crystallographic data. De novo predictive methods are distinct from other methods that rely on crystallographic information to be useful, in particular, "knowledge based" method, where a structural model for a protein whose structure is unknown is built by extrapolation from a structure of a homologous proteins whose crystal structure has already been solved T. L. Blundell, B. L. Sibanda, M. J. E. Sternberg, J. M. Thornton: "Knowledge-based Prediction of Protein Structures and the Design of Novel Molecules", Nature 1987, 326: 347-352!.

De novo methods in the prior art that attempt to predict the folded structure of proteins from sequence data fall into three general categories:

(i) Computational methods attempt to model the folded structure of proteins by calculating the relative energies of various possible conformations of the polypeptide chain in the search for the accessible conformation with the lowest energy. This method has been largely unsuccessful due to the large conformational space that a polypeptide chain can occupy, and difficulties in modeling the interaction of the protein with the solvent, water.

(ii) Statistical methods examine the proteins whose folded structures are known, and tabulate from these structures the probability that each of the 20 proteinogenic amino acids occurs in a particular secondary or supersecondary structure. In predictive work, these statistical structural propensities can be influenced by the sequence of the protein immediately before or after the amino acid residue in question, and can be averaged over an alignment of homologous protein sequences. Such methods normally are only partly successful, in part because the statistical preferences for individual amino acids to occupy particular secondary structures is small.

(iii) Methods based on physical chemical properties of the side chains of different amino acids place amino acid side chains that are hydrophilic outside the folded protein structure, where they are presumed to interact with solvent water, and hydrophobic side chains inside the folded structure. Secondary structures are assigned by patterns in the hydrophobicity or hydrophilicity of the side chains. For example, 3.6 residue periodicity is indicative of an alpha helix, while alternate periodicity is indicative of a beta strand. Such methods are only partly successful because evolutionary forces tend to introduce amino acid residues into polypeptide sequences that violate the 3.6 residue periodicity to achieve proteins with the desired level of conformational instability S. A. Benner, "Patterns of Divergence in Homologous Proteins as Indicators of Tertiary and Quaternary Structure," Adv. Enzym. Regulation, 28, 219-236 (1989)!.

From the large body of classical work, it is worth noting the classical work of Lim and of Schiffer and Edmundson, as the method of the present invention derives considerable inspiration from these authors. Schiffer and Edmundson M. Schiffer, A. B. Edmundson, "Use of helical wheels to represent the structures of proteins and to identify segments with helical potential", Biophys. J. 7, 121-135 (1967)! was the first to use helix wheels to illustrate the properties of alpha helices. Lim V. I. Lim, "Structural principles of the Globular organization of proteins chains: A stereochemical theory of globular protein secondary structure", J. Mol. Biol., 88, 857-872 (1974); V. I. Lim, "Algorithms for prediction of α-helical and β-structural regions in globular proteins", J. Mol. Biol., 88, 873-894 (1974)! was one of the first to formalize a method for identifying alpha helices in proteins structures that searches for a property of a polypeptide sequence that displays 3.6 residue periodicity.

In recent years, a number of papers appeared that transcended classical approaches, in that they have examined the sequences of more than one homologous protein in an effort to extract structural information. These papers have focused on the fact that homologous proteins have similar folded structures Chothia & Lesk, EMBO J. 5, 823 (1986); N. L. Summers, W. D. Carlson, M. Karplus, J. Mol. Biol. 196, 175 (1987)!.

For example, Crawford et al. I. P. Crawford, T. Niermann, K. Kirschner, "Prediction of Secondary Structure by Evolutionary Comparison: Application to the α Subunit of Tryptophan Synthase", Proteins, 2, 118-129 (1987)! examined a set of aligned homologous sequences of tryptophan synthase, and predicted that this protein folded to yield an eight fold alpha-beta barrel. This is, to the Applicant's knowledge, the first time that a correct de novo prediction has been made for the folded structure of a protein in advance of crystallographic data. Further, Crawford et. al. (1987) op. cit.! used gaps in the alignment to separate individual secondary structural elements. To assign secondary structure to each of these elements, Crawford et al. used a classical statistical algorithm developed by Gamier et al. J. Gamier, D. J. Osguthorpe, B. Robson, "Analysis of the Accuracy and Implications of Simple Methods for Predicting the Secondary Structure of Globular Proteins", J. Mol. Biol., 120, 97-120 (1978)!, assigned secondary structures to each homologous sequence individually, and then averaged the secondary structural assignments over all of the protein sequences to give an average secondary structure prediction. The secondary structure prediction had eight alpha helices interspersed with eight beta strands, a pattern well known in one class of protein fold, the eight fold alpha-beta barrel fold. This pattern was used by Crawford et al. to build a corresponding tertiary structure model for tryptophan synthase.

Zvelebil et al. M. J. Zvelebil, G. J. Barton, W. R. Taylor, M. J. E. Sternberg, "Prediction of Protein Secondary Structure and Active Sites using the Alignment of Homologous Sequences", J. Mol. Biol., 195, 957-961 (1987)! proposed a similar approach of averaging secondary structure predictions made by the method of Gamier et al. (1978) op. cit.!, but did not attempt to predict any unknown structures with this approach. Likewise, Taylor and Green W. R. Taylor, N. M. Green, "The predicted secondary structures of the nucleotide-binding sites of six cation-transporting ATPases lead to a probably tertiary fold", Eur. J. Biochem. 179, 241-248 (1989)! average secondary structure predictions made individually for each of a set of protein sequences to obtain a consensus secondary structure prediction, and use a knowledge-based approach to model a tertiary structure.

The main disadvantage of the method advocated by Crawford et al., Zvelebil et. al., and Taylor and Green is that it does not work well on a wide range of protein structures.

Patthy also suggested that gaps in an alignment of several homologous protein sequence can indicate which residues form surface loops L. Patthy, "Prediction of surface loops of protein-folds from multiple alignments of homologous sequences," Acta Biochim. Biophys. Hung. 24, 3-13 (1989)!, but did not attempt to predict any surface loops in any unknown structures with this approach.

Further, it is generally appreciated in the art that certain residues at the active site of an enzyme are highly conserved during divergent evolution. Zvelebil and Sternberg M. J. Zvelebil, M. J. E. Sternberg, Prot. Eng. 2, 127-138 (1988)! have reported an algorithm that scans for conserved polar residues in regions of high conservation in a multiple alignment as an indicator of active site residues. Of 16 active site residues in a test sample of alignments, 13 were correctly predicted, with an overprediction of 50 positions. This level of success is inadequate to permit a prediction of a structure using this algorithm as a component of the predictive method.

Overington et al. J. Overington, M. S. Johnson, A. Sali, T. L. Blundell, "Tertiary Structural Constraints on protein evolutionary diversity: Templates, key residues, and structure prediction" Proc. Roy. Soc. B.. 241, 132-145 (1990)! have studied patterns of sequence divergence in proteins with known crystal structures. Depiereux and Feytmans E. Depiereux, E. Feytmans, "Simultaneous and multivariate alignment of protein sequences: Correspondence between physicochemical profiles and structurally conserved regions", Prot. Eng., 4, 603-613 (1991)! have also noticed patterns in conservation of individual residues during divergent evolution. Neither have provided a method for predicting secondary or tertiary structure.

SUMMARY OF THE INVENTION

In the method of the present invention, a model for the folded structure of a set of proteins is built from a set of aligned homologous protein sequences. The method of the present invention is characterized by several operational features, each of which is essential for the method to be efficacious:

(i) The method of the present invention examines aligned sequences of a set of homologous proteins, rather than a single sequence of a single protein.

(ii) The method of the present invention extracts information concerning the three dimensional structure of the protein family from patterns of conservation and variation within a set of homologous sequences, not by a simple averaging of a property of the sequences taken individually.

(iii) The method of the present invention combines algorithms that assign positions in the alignment to the surface of the folded structure, to the interior of the folded structure, and to the active site, as a first step for predicting secondary structural elements.

(iv) The method of the present invention identifies separate secondary structural elements in the alignment using parsing algorithms that identify gaps in the alignment and specific parsing sequence motifs.

(v) The algorithms used by the method of the present invention are applied to subgroups of proteins with clearly identified evolutionary relationships, in particular, a clearly specified sequence identity and evolutionary distance.

(vi) The algorithms are designed to reflect how natural selection and neutral drift, two evolutionary processes, influence the divergent evolution of protein sequences.

(vii) The method of the present invention assembles the secondary structural elements to form supersecondary and tertiary structural models by orienting these elements using disulfide bridges, active site assignments, and covariation analysis.

Some aspects of the method covered by this application were discussed in an article published on Mar. 27, 1991 by the Applicant and a coworker who worked at the Applicant's direction S. A. Benner and D. Gerloff, "Patterns of Divergence in Homologous Proteins as Indicators of Secondary and Tertiary Structure: The Catalytic Domain of Protein Kinases," Adv. Enzyme Regulat., 31, 121-181 (1991)!. The publication date is less than one year prior to the date that this application is being filed.

The general philosophy underlying the method of the present approach was outlined in an earlier publication Benner (1989) op. cit.!. However, this publication covers only the preliminary work leading to the method of the present invention, was written prior to the reduction to practice, and neither makes obvious to nor enables one of ordinary skill in the art to practice the method of the present invention.

What allows the de novo predictive method that forms the present invention to be most clearly distinguished from the prior art is its efficacy. Specifically, the method disclosed here has been shown to provide remarkably reliable predictions for the folded structures of proteins whose structures are not known at the time that the prediction is made, but subsequently become known. Methods known in the prior art have not.

Illustrating the inefficacy of methods known in the prior art was a contest sponsored recently by the Protein Club. In this contest, laboratories developing and using classical methods for predicting the folded structure of proteins were invited to predict the folded structure of protein whose structures had been solved, but where the solution was withheld from the contestants. No de novo method successfully allowed the prediction of the folded structure. Wrote the workshop organizer, "The answer to the question `Structures from Sequences?` is `Not yet, unless you know what the structure looks like`" D. C. Rees, "Three-dimensional Protein Structure Prediction Workshop: Overview and Summary", Curr. Res. in Prot. Chem., NY, Academic Press, 551-556 (1990)!.

The efficacy of the method of the present invention was demonstrated by the prediction of a secondary structure for protein kinase in advance of any information regarding the crystal structure. The prediction was submitted for publication by Benner and Gerloff (1991) op. cit.! on Sep. 21, 1990, well in advance of the solution of the crystal structure by Sowadski, Taylor and their colleagues. The results of the crystal structure were published in July, 1991 D. R. Knighton, J. Zheng, L. F. Ten Eyck, V. A. Ashford, N. H. Xuong, S. S. Taylor, J. M. Sowadski: "Crystal Structure of the Catalytic Subunit of Cyclic Adenosine Monophosphate-dependent Protein Kinase", Science, 253, 407-414 (1991)!. The crystal structure showed that the prediction was remarkably accurate. Knighton et al. (1991) op. cit.! wrote:

"Although most of the predictions of secondary structure in the C subunit have been quite inaccurate and do not correlate well with the actual structure, the recent prediction by Benner and Gerloff is an exception. Their prediction of the secondary structure . . . is remarkably accurate, particularly for the small lobe"

Another indication of the inefficacy of the prior art and the efficacy of the method of the present invention comes from the paper of Thornton et al. J. M. Thornton, T. P. Flores, D. T. Jones, M. B. Swindells: "Prediction of Progress at Last", Nature, 354, 105-106 (1991)! published in Nature shortly after the prediction for protein kinase was confirmed by Knighton et al. (1991) op. cit.!. Thornton et al. noted that:

"Benner and Gerloff tackled secondary-structure prediction; this was essentially a case study of the catalytic domain of the protein kinases, the structure of which was then unknown. The cause for excitement is that the structure has since been solved by X-ray crystallography, and Benner and Gerloff s prediction of the core secondary structures was much better than that achieved by standard methods."

Another indication of the efficacy of the method of the present invention comes from Table 1, which summarizes the prediction for the catalytic domain of protein kinases. The details of the predictive success are reviewed by Benner Benner, "Predicting de novo the Folded Structure of Proteins", Curr. Opin.. Struct.. Biol., in press (1992), copy enclosed!, and by Thornton et al. (1992) op. cit.!.

The method is also operationally distinct from the prior art. Although Crawford et. al. (1987) op. cit.! made a prediction based on an analysis of an alignment of homologous sequences, they predict secondary structure by averaging secondary structural predictions made on individual sequences, rather than by extracting information concerning the three dimensional fold of the protein family from patterns of conservation and variation within the set of homologous sequences. This is the same approach advocated by Zvelebil et al. (1987) op. cit.! This approach has a particularly important disadvantage: it has been shown not to work well for many classes of protein fold other than the 8-fold alpha-beta barrel class.

Further, although Zvelebil and Sternberg (1988) op. cit.! extract information concerning the position of active site residues within a protein family from patterns of conservation and variation within a set of homologous sequences, their algorithms make no effort to to initially understand the evolutionary relationship between the sequences themselves as a precondition for interpreting patterns of conservation and variation in a fashion. This deficiency is responsible in part for the low reliability of the algorithms that these investigators have produced. In particular, missing from all of the prior art is operational feature (v). Those who analyze variation and conservation within a family of protein sequences seem universally to have overlooked the need to understand in a systematic way the evolutionary relationship between the sequences themselves as a precondition for interpreting conservation and variation in a fashion that is likely to be predictively useful. Also missing from all work in the prior art is operational feature (vi), a recognition that competing evolutionary processes, natural selection and neutral drift, will complicate the analysis of patterns of structural divergence in a protein family. Also missing from all work in the prior art is operational feature (vii), an approach towards building a model for the supersecondary and tertiary structure for a family of proteins. Although such model building is an art that is slightly different for each protein family examined, the method of the present invention did yield a correct model for the first domain of protein kinase Benner (1992) op. cit.; Thornton et al. (1991) op. cit.!, again before any crystallographic information was available.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1. Evolutionary tree describing the evolutionary relationship for a preferred set of proteins for implementing the method of the present invention for predicting the folded structure of proteins from a set of aligned homologous sequences. The idealized set of 16 proteins has 8 pairs of proteins, each pair 80% sequence identical. Numbers indicate the pairwise identity of the designated subgroups of proteins.

FIG. 2. The evolutionary tree relating the alcohol dehydrogenases whose sequences are aligned in Table 3. Numbers indicate the minimum pairwise identity of the designated subgroups of proteins.

FIG. 3. Helix wheels for segments of protein kinase: (a) Helix wheel covering positions 59-75 of protein kinase; (b) Helix wheel covering positions 114-123 of protein kinase; (c) Helix wheel covering positions 131-151 of protein kinase; (d) Helix wheel covering positions 259-273 of protein kinase; (e) Helix wheel covering positions 285-302 of protein kinase; (f) Helix wheel covering positions 318-326 of protein kinase.

FIG. 4. The predicted secondary, supersecondary, and tertiary structure for protein kinase.

FIG. 5. A model for the active site of protein kinase.

FIG. 6.(a) Evolutionary tree for the pathogenesis related proteins from plants;

a. PR14 pathogenesis-related protein from tobacco

b. Arabidopsis thaliana pathogenesis-related protein 1 (PR-1) gene

c. Lycopersicon esculentum PR (pathogenesis related) protein (P4)

d. Lycopersicon esculentum PR (pathogenesis related) protein (P6)

e. Tobacco PR-1a gene for pathogenesis-related proteins

f. Tobacco gene for pathogenesis-related protein 1b (PR1b)

g. Tobacco mRNA fragment for pathogenesis-related protein PR1c

h. Maize PRms gene for a pathogenesis-related protein

(b) Evolutionary tree for pathogenesis related proteins from plants and their homologues, the sperm coating and testis specific (TPX) proteins from mammals, and venom allergens from insects. The numbers indicate the approximate PAM distance between the evolutionarily most distant members of the indicated subgroups Gonnet et al. (1992) op. cit.!, that is, the number of accepted point mutations per 100 residues separating the most distant protein sequences.

a. Pathogenesis related leaf protein P14

b. Pathogenesis related protein 1A precursor (PR-1A).

c. Pathogenesis related protein 1B precursor (PR-1B).

d. Pathogenesis related protein 1C precursor (PR-1C).

e. Basic form of Pathogenesis related protein 1 precursor (PRP 1)

f. Sperm coating glycoprotein (SCP)

g. Testis specific protein TPX-1 precursor (gene name: TPX1)

h. Testis specific protein TPX-1 precursor

i. Venom allergen 5 form 2 precursor (AG5-2).

j. Venom allergen 5 form 3 precursor (AG5-3)

FIG. 7. Helix wheels for segments of the pathogenesis related proteins from plants, derived from the alignment in Table 16. (a) Helix wheel covering segment 1 of PR proteins; (b) Helix wheel covering segment 2a of PR proteins; (c) Helix wheel covering segment 2b of PR proteins; (d) Helix wheel and beta template covering segment 3 of PR proteins; (e) Helix wheel covering segment 4ab of PR proteins; (f) Helix wheel covering segment 4cd of PR proteins; (g) Helix wheel and beta template covering segment 5 of PR proteins; (h) Helix wheel and beta template covering segment 6 of PR proteins.

FIG. 8. Helix wheels for segments of the pathogenesis related proteins from plants and their homologues, derived from the alignment in Table 17. (a) Helix wheel covering segment 2a of PR proteins; (b) Helix wheel and beta template covering segment 2b of PR proteins; (c) Helix wheel and beta template covering segment 3 of PR proteins; (d) Helix wheel covering segment 4a of PR proteins; (e) Helix wheel covering segment 4c of PR proteins; (f) Helix wheel and beta template covering segment 5 of PR proteins; (g) Helix wheel and beta template covering segment 6 of PR proteins.

FIG. 9. The predicted secondary structure for pathogenesis related proteins from plants and their homologues.

DETAILED DESCRIPTION OF THE INVENTION AND PREFERRED EMBODIMENTS

A glossary of abbreviations and terms is given below.

Alignment Anchor: A position in an alignment that is sufficiently conserved across the entire alignment, or undergoes only conservative substitution, such that the alignment of the sequences at this position is highly reliable.

Amphiphilic split in a cluster of subgroups with MPI of X: Designates a position in an alignment where none of the subgroups in the cluster of subgroups at MPI=X is variable, where at least one subgroup contains a hydrophobic residue and at least one subgroup contains a hydrophilic residue.

APC (all proteins conserved): Designates a position in an alignment where all of proteins being considered have the same amino acid at that position.

Clusters of subgroups with an MPI=X: The proteins in the alignment are divided into subgroups with different overall levels of sequence identity. All subgroups with a particular MPI belong to a particular cluster of subgroups.

CMX Y (Count minus X): Designates a position in the alignment where all but X proteins have amino acid Y.

Distributed parse: A parse built from parsing elements that appear in different subgroups of the alignment at neighboring position numbers.

Functional subgroups: The proteins in the alignment are divided into 12 functional subfamilies, where members of each functional subgroup (or subfamily) share among themselves, and are distinct from members of other functional subgroups, a particular biological function or catalytic behavior.

Helix Wheel: A projection of a helix down its long axis, showing the directions that side chains at different positions along the helix protrude Schiffer & Edmundson (1967) op. cit.!

Hydrogen bonding variable at MPI=X: Designates a position in an alignment with more than one variable subgroup in the cluster of subgroups with MPI=X, where at least some amino acids in the subgroups have an indifferent residue at this position (C, H, Q, S, T), but no subgroups have a polar residue at this position (D, E, K, N, R).

Hydrophilic split in a cluster of subgroups with MPI of X. Designates a position in an alignment where the each subgroup displays no variation within subgroup, and all amino acids in each subgroup are either hydrophilic or indifferent.

Hydrophobic anchor for an external loop: A position with a hydrophobic amino acids in most proteins appearing in a segment that is a parse or otherwise assigned as a surface loop.

Hydrophobic residue: An amino acid selected from the group consisting of Phe, Ile, Leu, Met, Val, Trp, and Tyr.

Hydrophobic split in a cluster of subgroups with MPI of X. Designates a position in an alignment where none of the subgroups in the cluster of subgroups at MPI=X is variable, and all amino acids in each subgroup are either hydrophobic or indifferent.

Hydrophobic variable in a cluster of subgroups with MPI of X: Designates a position in an alignment where none of the subgroups in the cluster of subgroups at MPI=X is variable, but where none of the subgroups has either an indifferent or a polar residue (C, H, Q, S, T, D, E, K, N, R).

Indifferent residue: An amino acid selected from the group consisting of Ala, Cys, Gly, His, Pro, Gln, Ser, and Thr.

Inside arc: The side of a helix wheel from which protrude side chains of residues assigned to the inside of the folded protein structure.

MPI=Minimum pairwise identity. The MPI value for a subgroup of proteins in the alignment is the percent sequence identity of the two least similar proteins in the subgroup. Increasing MPI corresponds to decreasing PAM distance connecting the most distantly related proteins the subgroup.

Neutral split in a cluster of subgroups with MPI of X: Designates a position in an alignment where none of the subgroups in the cluster of subgroups at MPI=X is variable, where every subgroup contains an indifferent residue.

Non-standard secondary structure: All secondary structures other than an alpha helix or a beta strand (e.g., a 3₁₀ helix or a collagen helix).

PAM distance (accepted point mutations): A measure of the evolutionary distance between two proteins, where the PAM distance is the most probable number of accepted point mutations separating the two sequences per 100 amino acid residues, corresponding to the number of times the first sequence must be transformed by a 1% mutation matrix (a mutation matrix where the sum of all off diagonal elements is such that transformation of a sequence by this matrix yields a protein with 1 mutation per 100 amino acids) to yield the second protein with the highest probability.

Parse: A segment that divides the alignment into segments whose secondary structure is considered separately.

Parsing string: A sequences of consecutive amino acids in a single protein that indicates that the segment lies between standard secondary structural units (e.g., GG, PP, PG, GP, NN, NS, etc.).

Polar residue: An amino acid selected from the group consisting of Asp, Glu, Arg, Lys, and Asn.

Reflexivity: Designates a position in an alignment where patterns of variation among the proteins being examined suggests a tree-like relationship between these proteins that is different from the evolutionary tree derived from examination of the entire sequences of the proteins. Most commonly, a position displays reflexivity when the pattern of variation involving particular amino acids is the same in two distant subgroups.

Split at MPI=X: Designates a position in an alignment where none of the subgroups in the cluster of subgroups at MPI=X is variable.

Standard secondary structural elements: an alpha helix or a beta strand.

String: A set of consecutive positions in the alignment

Subgroup with an MPI=X: A subset of the proteins in an alignment that bears a specified evolutionary relationship, that the sequences have a minimum pairwise identity of X percent.

Surface arc: The side of a helix wheel from which protrude side chains of residues assigned to the surface of the protein.

Variable subgroup: At a position in an alignment, a subgroup of proteins in the alignment where more than one kind of amino acid is present.

Throughout this discussion, the one letter code for amino acids is used (Table 2).

Step 1. Obtain an Alignment

Sequences of a set of homologous proteins must be obtained and aligned. Alignments of the sequences of homologous proteins may be obtained using one of many algorithms well known to those skilled in the art, and summarized in volume 183 of Methods in Enzymology R. F. Doolittle, editor, Methods Enzymol. 183, 1-736 (1990)!.

To be useful in structure prediction work, the alignment must meet a simple criterion: The alignment should match as much as possible homologous amino acid residues in different proteins, that is, amino acids from two proteins placed in apposition in the alignment must be descendents of a single codon in the most recent common ancestral protein. This criterion is easily met in proteins having high sequence similarity. For proteins with lower sequence similarity, this normally requires accurate placement of gaps in an alignment. Operationally, this requirement can normally be met with classical alignment algorithms by incorporating into an alignment only protein pairs that have a minimum pairwise identity of at least 35%. More divergent proteins can be aligned successfully using a deletion gapping penalty derived from an exhaustive matching of the protein sequence data base G. H. Gonnet and S. A. Benner, "Computational Biochemistry Research at ETH", Technical Report I54, Departement Informatik, March (1991); G. H. Gonnet, M. A. Cohen, S. A. Benner, "Exhaustive Matching of the Entire Protein Sequence Database", Science in press (1992), copy enclosed!. However, when pairwise identity falls below ca. 25%, the reliability of an alignment drops below the point where is is useful in the method of the present invention.

Further, to be useful, an alignment must contain at least 4 homologous protein sequences, and include some pairs that are closely similar in sequence (more than 80% identical in sequence), some pairs that are moderately similar in sequence (40-80% pairwise identity), and some that have distant similarity (less than 40% pairwise identity). More preferably, an alignment should contain at least 8 homologous protein sequences, which consist of 4 subgroups of proteins consisting of pairs of sequences with 80-85% minimum pairwise identity, where these subgroups pairwise form two subgroups with an intersubgroup pairwise identity of 45-55%, and with the subgroup containing all of the protein sequences having a minimum pairwise identity of 25-35% (FIG. 1). Most preferably, the alignment should be as large as possible, and the evolutionary tree relating the proteins in the alignment should be highly branched. For example, with protein kinase (Example 1), a successful prediction was made with over 70 proteins in the alignment.

Should the appropriate homologous sequences not be available in sequence databases, they can be obtained by molecular biological methods well known to those skilled in the art, using one of the available sequences as a probe for new homologous sequences in appropriately chosen libraries. Branches are added at the appropriate positions to an evolutionary tree by selecting the organisms from which additional sequences are cloned, based on the estimated rate of divergence of the protein sequence, and the estimated time of divergence of the organisms that provide the additional sequences.

Step 2. Divide the alignment into subgroups with different overall sequence similarity.

A key element of the method of the present invention is based on the recognition that information regarding folding patterns appears and disappears at different points during the divergent evolution of a set of proteins. Thus, variation and conservation in a set of homologous protein sequences can be interpreted only in the context of the overall sequence similarity of the proteins being examined. Thus, the "minimum pairwise identity" (or MPI) within a subgroup of proteins must be known to evaluate the significance of any conservation or divergence seen within the subgroup. The structural significance of the similarities and differences between the sequences of a pair of homologous proteins depends on the overall sequence divergence of the pair, according to simple rules. For two closely homologous proteins that are similar in sequence, the differences in the sequences are most significant. For two distantly homologous proteins that are different in sequence, the similarities in the sequences are most significant. Thus, before structural information can be extracted from an alignment, it must be divided into subgroups with different minimum pairwise identities (MPI).

For example, a set of sequences of homologous alcohol dehydrogenases contains 17 proteins. An alignment of these sequences is shown in Table 3; an evolutionary tree relating these sequences is shown in FIG. 2. At MPI=85%, there is a cluster of 10 subgroups of proteins in the alignment. At MPI=50%, however, there are only 2 subgroups.

Step 3. Divide the sequence into segments using parsing algorithms

The core of any structural analysis is a discussion that, from the beginning to the end of an alignment, examines every structural feature of the protein family, one position at a time. To simplify the discussion, the alignment is divided into manageable pieces using "parsing elements", or "parses". Parsing algorithms are intended to identify segments that lie between standard secondary structural units (e.g., alpha helices or beta strands), allowing the biological chemist to consider the secondary structure of the segments between parses individually.

Parsing algorithms have different degrees of reliability. The parsing algorithms find the positions in the alignment that have following structural features, and assign breaks in the secondary structure at these positions. The algorithms are illustrated in Table 4, and listed below in order of decreasing reliability:

(i) Gap parse. The strongest algorithm parses the alignments at points whether there is a gap, that is, at position where the alignment shows that one or more proteins in the family of homologous proteins has undergone an insertion or deletion during divergent evolution. As a rule, insertions and deletions are generally not accepted by natural selection within standard secondary structural elements (alpha helices and beta strands). Thus, the presence of a gap in an alignment indicates that this region is not part of one of these secondary structural units. Some precedence for this parsing algorithm can be found in the literature Crawford et al. (1987) op. cit.!. In the cases where there is a parse via a gap, it is occasionally useful to have an assignment (surface or inside) for those sequences that are present (vide infra).

(ii) APC P parse. While a proline present in a single sequence is not a good indicator of a break in a secondary structural unit, a proline that is conserved in a set of proteins having an MPI below 40% is a strong indicator of a break in standard secondary structure.

(iii) Distributed parse. When parsing elements (e.g., a Pro or a Gly) is not conserved at a single position in all of a set of proteins having an MPI below 40%, but is found at adjacent or closely adjacent positions in these proteins, this is also a reliable indicator of a break in standard secondary structure.

(iv) APC G parse. While a glycine present in a single sequence is not a good indicator of a break in a secondary structural unit, a proline that is conserved in a set of proteins having an MPI below 40% is an indicator of a break in standard secondary structure. The parsing element is not absolute. APC G can be found as an inside residue in a standard secondary structure as well.

(v) Combination parse. A combination of Pro, Gly, Asn, Ser, or Asp conserved across the entire alignment.

(vi) Distributed combination parse: A distributed combination of Pro, Gly, Asn, Ser, or Asp.

(vii) String parse. Certain dipeptides (GG, GP, PG, PP) almost always indicate parses, especially if conserved in subgroups with an MPI of 85% or lower. Other dipeptides (NN, SS, NS, SN, NG, GN, GS, SG, PN, NP, PS, SP) occasionally indicate parses, especially if conserved in subgroups with an MPI of 70% or lower.

Depending on the size of the alignment, it is often possible to use only the strongest parsing algorithms to assign breaks in secondary structure. This is the case with Example 1. With alignment containing only a few proteins, weaker parsing units must be considered, at least tentatively. This is illustrated in Example 2. In every case, parsing decisions can be revised in light of data collected at later stages in this procedure; occasionally, the alignment is also slightly modified, as alignments are generally least accurate near regions of gaps (Example 2). This revision is necessary because, although the parsing algorithms are highly accurate, they are not perfectly accurate. Approximately once every 400 positions in a typical alignment, a deletion occurs within a secondary structural element. For example, in alcohol dehydrogenase (ADH), a deletion position 256 in the horse liver enzyme, in a helical region. Finally, the alignment is the weakest in regions of surface loops (which are normally the most rapidly diverging segments of a protein). Thus, refinement of the assignments of parses involves three issues:

(i) Where are the beginning and end of the parse?

(ii) Is the parse confirmed by segments within the insertion?

(iii) Can the deletion or insertion be removed by shifting of the alignment itself?

To confirm a gap parse, the segments of the sequences of proteins that do not have a deletion are examined for weaker parsing elements, for example, consecutive G's, PXP motifs, and PG strings, which are common in external loops that are prone to deletion during divergent evolution.

Point (iii) addresses features of the alignment itself. The most preferable alignment programs have a deletion penalty obtained by an exhaustive matching of the entire protein sequence data base Gonnet et al. (1992) op. cit.!, which places a high cost on deletions.

Finally, errors in an alignment can juxtapose amino acids in different proteins that are not true homologs, that is, are not descendent from the same codon in a common ancestor. These errors create problems at several levels of the analysis, and the alignment therefore must be reevaluated throughout the structural modelling. Examination of the alignment focuses on alignment anchors, positions where the conservation across the entire alignment is sufficiently high that there can be no question that the alignment is correct at this position. The alignment is then built out from the anchors towards other anchors, as exemplified below.

Step 4. Assign Surface Positions

Positions in the alignment that contain amino acids whose side chains lie on the surface of the folded structure are identified by surface algorithms. The development of surface algorithms requires a sophisticated understanding of the processes by which protein sequences divergently evolve, and it is instructive at this point to consider both an evolutionarily naive and an evolutionarily sophisticated approach to constructing such algorithms.

In a naive approach, variation of the amino acid side chain at a position in an alignment can be taken as an indication that the side chain at that position protrudes into solvent (water). The logic is straightforward. A residue that protrudes into solvent can be more easily changed without disrupting the function of the protein, because it does not form contacts with other protein residues whose position might be disrupted by a change in the size or functionality of the residue in question.

This approach is naive at two levels. First, the significance of variation in an alignment depends, as noted above, on the overall divergence of the protein sequences being examined. The more sequence divergence that has taken place between two proteins, it is less likely that this variation is an indicator of a surface position, as it is more likely that internal variation is compensated by other internal variation. Obviously, the most reliable surface assignments are based on variation between two proteins that have an high overall level of sequence simliarity.

Simple empirical evaluation demonstrates that even if adjusted for overall variation among the proteins being examined, an algorithm that assigns positions to the surface based simply on variation is unreliable. For example, in alcohol dehydrogenase, if one assigns a position in the alignment to the surface if it displays variation in a subgroup of proteins with an MPI=85%, of 174 positions assigned to the surface, only 106 (61%) are correctly assigned; 68 (39%) of the positions do not in fact lie on the surface. In this protein, if assignments were made at random, they would be 50% correct. Thus, the predictive power of the naive algorithm is little better than random.

It is easy to explain why this algorithm is bad: It is based on an incorrect understanding of how proteins evolve. During divergent evolution, two types of variation can occur. The first is neutral variation, substitutions of one amino acid by another that do not alter the behavior of a protein to an extent sufficient to influence the ability of a host organism to survive and reproduce. The second is adaptive variation, substitution of one amino acid by another that is selected for precisely because it does alter the behavior of a protein to an extent sufficient to influence the ability of a descendent organism to survive and reproduce in a new environment. Neutral variation and adaptive variation have exactly opposite implications, both in terms of impact on the behavior of a protein, and its position in the folded structure.

Neutral variation lies predominantly on the surface of the protein, where it does not influence the behavior of a protein to an extent that will influence survival. However, adaptive variation is supposed to alter the behavior of a protein. Therefore, it does not necessarily occur on the surface. Adaptive variation can occur anywhere in the folded structure, including inside the folded structure, or at the active site.

The naive algorithm fails because it treats all variation in an alignment as neutral variation. Indeed, a key teaching of the present invention is that for a method to successfully extract structural information from an alignment of homologous sequences, algorithms must be constructed to distinguish neutral and adaptive variation.

The discovery that underlies the surface predictions used by the method of the present invention distinguishes neutral variation from adaptive variation by patterns of concurrent polar variation. As with parsing algorithms, surface algorithms have different degrees of reliability. The surface algorithms find the positions in the alignment that have following structural features, and assigns the side chains of amino acids at these positions to the surface of the proteins. The algorithms are illustrated in Table 5, and listed below in order of decreasing reliability:

(i) Surface Algorithm 1: Concurrent polar variation in n subgroups: In a cluster of subgroups with a defined MPI, variation at a position concurrently in n subgroups at a defined MPI, where n is two or greater, when at least one of the variable subgroups contains a hydrophilic amino acid (Arg, Lys, Asp, Glu, Asn). The reliability of the algorithm increases with increasing n, increasing MPI, and increasing number of polar residues. Preferably n is 2 or greater, and MPI is 80% or greater. The larger the number of sequences in the alignment (other factors being equal), the greater the fraction of the surface positions that are assigned using this algorithm.

(ii) Surface Algorithm 2: Concurrent variation in n subgroups matched against a polar residue: In a cluster of subgroups with a defined MPI, variation at a position concurrently in n subgroups at a defined MPI, where n is two or greater, when at least one of the nonvariable subgroups contains a hydrophilic amino acid (Arg, Lys, Asp, Glu, Asn). The reliability of the algorithm increases with increasing n, increasing MPI, and increasing number of polar residues. Preferably n is 2 or greater, and MPI is 80% or greater. The larger the number of sequences in the alignment (other factors being equal), the greater the fraction of the surface positions that are assigned using this algorithm.

(iii) Surface Algorithm 3: Polar single variable: In a cluster of subgroups with a defined MPI, variation in one subgroup, where the variable subgroup contains at least one a hydrophilic amino acid side (Arg, Lys, Asp, Glu, Asn), and the non-variable subgroups contain no amino acids other than (Ala, Cys, Asp, Glu, Gly, His, Lys, Asn, Pro, Gln, Arg, Ser, or Thr). The reliability of the algorithm increases with increasing n, increasing MPI, and increasing number of polar residues. The larger the number of sequences in the alignment (other factors being equal), the greater the fraction of the surface positions that are assigned using this algorithm.

(iv) Surface Algorithm 4: Polar Split: In a cluster of subgroups with a defined MPI, all subgroups are non-variable, all subgroups contain no amino acids other than (Ala, Cys, Asp, Glu, Gly, His, Lys, Asn, Pro, Gln, Arg, Ser, or Thr), and at least one subgroup containing one of the amino acids (Arg, Lys, Asp, Glu, Asn).

Depending on the branching of the evolutionary tree of the proteins in the alignment, it is often possible to use only the strongest surface algorithms to identify a majority of the positions that lie on the surface of the folded structure. This is the case with Example 1. With alignment containing only a few proteins, weaker surface algorithms must be used. This is illustrated in Example 2. In every case, surface assignments can be revised in light of information developed at later stages in this procedure. This revision is necessary because, although the surface algorithms are highly accurate, they are not perfectly accurate. For example, Table 6 shows data for estimating the quantitative reliability of Surface Algorithm 1 for the protein alcohol dehydrogenase (Adh)

Step 5. Assign interior positions

Positions in the alignment that contain amino acids whose side chains lie inside the folded structure are identified by interior algorithms. These algorithms are in many respects the converse of surface algorithms. They identify conservation (rather than variation), and hydrophobic residues (rather than polar residues). The significance of conservation is greater in subgroups with lower MPI values than with high MPI values. As with other algorithms, interior algorithms have different degrees of reliability. The surface algorithms find the positions in the alignment that have following structural features, and assigns the side chains of amino acids at these positions to the surface of the proteins. The algorithms are illustrated in Table 7, and listed below in order of decreasing reliability:

(i) Interior Algorithm 1: APC hydrophobic. A hydrophobic residue (A,F,I,L,M,V,W,Y) conserved in all proteins in an alignment. The reliability of this algorithm decreases with increasing MPI.

(ii) Interior Algorithm 2: Hydrophobic variable. In a cluster of subgroups with a defined MPI, variation at a position concurrently in n subgroups at a defined MPI, where n is two or greater, when no subgroup contains a hydrophilic amino acid (Arg, Lys, Asp, Glu, Asn) or a neutral amino acid (C,H,Q,S,T). The reliability of this algorithm increases with decreasing MPI for all proteins in the alignment.

(iii) Interior Algorithm 3: Hydrophobic split. In a cluster of subgroups with a defined MPI, a position where no subgroup displays variability, and where all subgroups contain either hydrophobic or neutral residues at this position. The reliability of this algorithm increases with decreasing MPI, and with increasing numbers of proteins in the alignment.

(iv) Interior Algorithm 4: Hydrophobic single variable. In a cluster of subgroups with a defined MPI, variation in one subgroup, where all subgroups contain only hydrophobic residues.

(v) Interior Algorithm 5: Preponderance of hydrophobic residues. In all proteins at this position, a high percentage (>85%) contain a hydrophobic amino acid. The reliability of this algorithm increases with decreasing MPI for all proteins in the alignment, and with increasing percentage of hydrophobic residues.

Step 6. Assign active site residues

Side chains bearing functional groups (C,D,E,H,K,N,Q,R,S,T,Y) that are conserved across the entire alignment are tentatively assigned to an active site. Cysteines involved in the formation of disulfide bridges are excluded in this analysis. This assignment is rigorous only when the overall divergence of the family of proteins contains pairs of sequences with a pairwise identity of less than 50%, more preferably less than 35%. The assignment is most reliable for C, H, and S. Charged side chains (D, E, K, and R) are often conserved during divergent evolution because they play a structural role in the folding of the polypeptide chain. T and Y are only occasionally at the active site; they are often highly conserved when they are part of a core structure. Thus, all active site assignments must be confirmed.

Confirmation of active site assignments involves identifying conserved strings in subgroups of proteins with different overall minimum pairwise identities. In subgroups with a MPI of 30-45%, APC dipeptide strings are indicators of an active site; APC tripeptides are strong indicators. In subgroups with a MPI of 45-60%, APC tripeptide strings are indicators of an active site; APC tetrapeptides are strong indicators. In subgroups with a MPI of 60-70%, APC tetrapeptide strings are indicators of an active site. The conserved strings can be broken by 1 or 3 interspersed non-conserved positions; in these cases, an active site beta strand and an active site helix are indicated, respectively. In subgroups of proteins having higher MPI values, longer conserved strings can occasionally be used to confirm an active site assignment. However, in these cases, conservation is often so widespread in such subgroups as to make such an analysis not particularly valuable. The strongest active site assignments are those based on an APC functional residue embedded in an APC dipeptide in a subgroup with MPI=30-45%, which is in turn embedded in a APC tripeptide in other subgroups of the same alignment with a MPI of 45-60%, which is in turn embedded in a APC tetrapeptide in other subgroups of the same alignment with a MPI of 60-70%.

Active sites create problems in assigning secondary structures, as the constraints on divergence arising from a catalytic or binding role are generally greater than those that manifest themselves in the patterns of conservation and variability that indicate a particular standard secondary structural element. Thus, active site segments are generally assigned an active site coil designation, unless the pattern of active site assignments themselves indicates a 3.6 residue or 2 residue periodicity.

Occasionally special circumstances in a family of proteins can be used to assign active site positions. This is especially the case when the function of members of a protein family has diverged, and patterns of variation in sequences can be detected that are responsible for this divergence of function can be identified. While these assignments are normally unique for each class of protein studied, and are illustrated for alcohol dehydrogenase in Benner (1989), op. cit.!.

Finally, knowledge of the catalytic function of an enzyme can suggest to one skilled in the art particular catalytic roles for individual active site residues. Often, these roles imply a relative spatial arrangement of these residues, and hypotheses concerning this spatial arrangement can help constrain the folding of a polypeptide chain.

Step 7. Assign secondary structure.

If a secondary structural unit sits in the folded form asymmetrically with respect to functional or structural features, then alpha helices and beta strands will make themselves apparent by 3.6 and 2 residues periodicity in the patterns of conservation and variation. The strongest assignment is made when the functional constraints on sequence divergence are the most asymmetrically disposed with respect to the two sides of the secondary structural unit. Thus, alpha helices that lie in the folded structure at the interface between the solvent and the core of the protein are essentially completely found by the method of the present invention, and no segments that are not surface helices are assigned as surface helices by this method.

In this approach, a dialectic method is used. First, a "canonical" assignment of secondary structure is made using simple rules. Other assignments are then said to have the burden of proof, meaning that the canonical designation is accepted unless the alternative designation is supported by the preponderance of evidence. In attempting to have other assignments meet this burden, the biological chemist is expected to construct as strong an argument as possible to set in opposition to the canonical assignment. This includes making ad hoc assumptions, restructuring the alignment, and questioning the assignments of positions to the surface and the interior of the protein.

Standard procedure in attempting to detect a helical segment involves mapping the surface/interior positions on a helix wheel. Should a pattern of amphiphilicity not be seen, positions are dropped from the ends of the segment, working towards the center, to determine whether the segment contains a subsegment that does display a 3.6 residue periodicity in conservation or variation. A canonical helix is assigned if amphiphilicity is seen over a segment 6 positions or longer. The effect of this procedure is to drop surface assignments at the ends of a helix when they fall in the "inside" face of the helix; this is not irrational, as many positions on the hydrophobic side of an amphiphilic helix lie on the surface when they are at the end of the helical structure. Further, this procedure has the effect of dropping loops with hydrophobic anchors at the end of helical segments.

If the pattern remains non-amphiphilic, the helical wheel is examined to identify assignments that, if changed, would create an amphiphilic helix. This provides a set of ad hoc assumptions (e.g., the helical assignment can be made if a particular assignment is incorrect). Then, the procedure is begun again, but this time beginning with the strongest assignments and working towards the weakest. The goal of this process is to find the strongest possible argument that the segment is helical. Quite often, the segment will be assigned to some other structure canonically, and this argument will serve only to establish a dialectic with respect to the canonical assignment.

There are several reasons why positional assignments might be incorrect. For example, in multimeric proteins, the most common interior misassignments are positions that lie on the surface of a subunit but form a subunit-subunit contact. The biological chemist must be aware of these complications when building models. Indeed, in alcohol dehydrogenase, such ambiguities can be used to advantage, as subgroups within the protein family are dimers while others are tetramers, and contact sites can be predicted based on patterns of sequence divergence and conservation (Benner (1989) op. cit.). Analogous problems are also expected when the protein interacts with a membrane.

The standard procedure for assigning a beta strand is to map a segment on an alternating template. Beta strands are rarely as obvious as alpha helices by this process when the entire alignment (with MPI<40%) is examined. Therefore, several expedients are adopted.

First, beta strands are often internal structures, and therefore appear as strings 2-8 positions long with consecutive interior assignments. M. J. E. Sternberg and J. E. Thornton, "On the conformation of proteins: An analysis of beta-pleated sheets", J. Mol. Biol. 110, 285-296 (1977)!. A hydrophobic stretch of this length is canonically assigned as an interior beta strand. Further, the pattern of variability of a putative internal beta segment is apparent as a progression from less conserved to more conserved to less conserved, with this pattern superimposed on an alternating pattern. Such structures are normally highly ordered, with splits at MPI>50%, and are not easily mistaken for surface helices or coils and loops.

Nevertheless, two major ambiguities remain in our approach in assigning beta strands. First, very short beta segments (2 amino acids) are generally difficult to assign correctly. Second, surface beta strands are often confused with surface coils. The latter may be an intrinsic limitation of any method that builds structural models from a set of homologous proteins, as the conformation of surface beta strands is not necessarily conserved during divergent evolution.

A string of four consecutive surface assignments is canonically assigned as a surface loop, an assignment that is rationalized by the classical argument that four consecutive polar amino acids in a single protein sequence indicates a surface loop or turn F. E. Cohen, R. M. Abarbanel, I. D. Kuntz, and R, J. Fletterick, Secondary structure assignment for alpha/beta proteins by a combinatorial approach, Biochemistry 22, 4894-4904 (1983); F. E. Cohen, R. M. Abarbanel, I. D. Kuntz, R, J. Fletterick, "Turn prediction in proteins using a pattern-matching approach", Biochemistry 25, 266-275 (1986)!. Often a surface loop is observed to have a single position where a hydrophobic amino acid is found (often hydrophobic variable). This is termed a "hydrophobic anchor for the loop, and serve as indicators of short loops.

In assigning secondary structures to parsed segments that are not coils, it is useful to classify the segment by the number of surface and interior assignments that it contains. This is done below.

Segments containing both surface and inside assignments. Such structures can be surface helices, surface beta strands, or surface coils. If the segment is longer than 6 positions, the possibility that it forms a surface helix is first considered. The entire segment is diagrammed onto a helix wheel. Then, subsegments are sought where the surface and inside assignments are rigorously amphiphilic. The positions at the beginning and end of the subsegment that break amphiphilicity (position i_(N) and i_(C)) are then identified. A helix is tentatively assigned between positions i_(N) and i_(C), with the "inside arc" being that side of the helix wheel from which protrude positions assigned to the inside, and the "surface arc" being that side of the helix wheel from which protrude positions assigned to the surface protrude. This is the primary tentative helix assignment.

To generate the secondary tentative helical assignments, the positional assignments that break amphiphilicity are assumed to be incorrect. Starting with the helix wheel generated above, two helical wheels are constructed, one continuing towards the amino terminus, the other continuing towards the carboxyl terminus. The next assignments that break amphiphilicity next found. If these amphiphilicity breakers are at position i_(N) -1 or i_(N) -2 (continuing towards the amino terminus), or at position i_(C) +1 or i_(C) +2 (continuing towards the carboxyl terminus), the tentative helix is terminated at positions i_(N) and i_(C), and no secondary tentative helix assignments are made. If an amphiphilicity breaker is at position i_(N) -3 or i_(N) -4 (continuing towards the amino terminus), or i_(C) +3 or i_(C) +4 (continuing towards the carboxyl terminus), a second, longer tentative helix is also tabulated beginning and ending at these positions.

Should the pattern of amphiphilicity not be broken a positions i_(C) -1, i_(C) -2, i_(C) -3 or i_(C) -4 (continuing towards the amino terminus), or i_(C) +1, i_(C) +2, i_(C) +3 or i_(C) +4 (continuing towards the carboxyl terminus), the helix diagrams are extended in both directions until another amphiphilicity breaker is encountered, and the process repeated.

The tentative helix assignments are confirmed and refined based on the fact that during divergent evolution, helices can undergo partial reorientation with respect to the folded structure. This reorientation occasionally manifests itself in the pattern of conservation and divergence in the alignment, and when such manifestations are observed, they can confirm a helix assignment, and allow the biological chemist to refine the extent of the helix assignment.

This process focuses on assignments that break amphiphilicity (inside assignments that fall in the surface arc, or surface assignments that fall in the inside arc), and assignments that lie in the two boundary regions between the surface arc and the inside arc, especially amphiphilic splits in these regions. The alignment is divided into subgroups, and inside and surface assignments are made for the subgroups of proteins individually where those assignments are consistent within the subgroup. Then, helix wheels are constructed to determine whether the misassignments apply to only some subgroups of proteins in the alignment and whether the misassignments can be reconciled with different helix orientations and/or different helix lengths in different subgroups of proteins in the alignment.

In some cases, a single turn at the end of a helix has been deleted during divergent evolution. A gap of 3 or 4 amino acids at the end of a putative helix can support a helix assignment.

Should a 3.6 residue amphiphilicity not be observed, and if the segment is shorter than 7 amino acids, an assignment as a coil or a beta strand is plausible. Each segment is also diagrammed on an alternating strand template to detect patterns of 2 residue periodicity that indicates a beta strand. Alternating inside and surface assignments that continue for 4 or more positions are assigned a surface beta structure.

More common with beta strands is a subsegment of 3 or more internal residues embedded in a parsed segment 10 positions or shorter. Again if no 3.6 residue periodicity is observed, a beta strand is assigned to the subsegment. This assignment can be confirmed by looking for 2 position periodicity in the parts of the segment flanking the subsegment.

Segments that have no surface assignments. Consecutive internal positions 3-6 amino acids long are assigned as beta strands, with the assignment confirmed as noted above.

Extended internal segments are either internal helices or a set of internal internal beta strands. The simplest approach for identifying internal helices is 3.6 position periodicity, especially that involving APC positions, splits, or active site residues. Alternatively, internal beta strands often manifest 2 position periodicity in this respect.

The most difficult important problem arises when an extended internal structure displays neither periodicity with statistical significance. The key to distinguishing between an internal alpha helix and an internal beta strand in this case comes from an estimate of the dimensions of the folded structure of the domain. This information can be obtained experimentally for single domain proteins, by sedimentation, diffusion, viscosity, or other methods known to those in the art. In the absence of experimental data, a single domain protein is assumed to be spherical, and the size of the core structure estimated from the length of the core sequence. For multidomain proteins, or in the absence of experimental information, domain size is assumed to be 20-30 Å across.

Underlying the assignment of internal structures is the generalization that secondary structural elements (alpha helices, beta strands) do not end in the middle of a folded structure. A beta strand traverses 3.2 to 3.4 Å per residue; an alpha helix traverses 1.5 Å per residue. A typical domain size is 20-30 Å across. Thus, a helix that traverses the domain must be no shorter than 13 amino acids. In contrast, an beta strand that traverses the domain is no longer than ca. 10 amino acids. A shorter helix can, of course, traverse a domain obliquely. However, amphiphilicity is generally seen with such helices at the ends.

Thus, if the extended internal segment is 13 amino acids or longer, an internal helix is assigned. If it is less than 13 amino acids, an internal beta strand is assigned. These assignments are canonical, and alternative assignments can be made if they meet a burden of proof. The most plausible alternative assignment for an extended internal segment longer than 13 positions is a set of bent beta strands. In these cases (Example 1), secondary parses are sought that might break the beta strand. Further, in cases where the segment passes near the active site, parsing elements are possibly obscured. In this case, the structure may be bent at the active site position.

Extended segments that have no inside assignments. Extended stretches having no inside assignments are assigned as surface coils Cohen et al. (1983) op. cit.!. Usually such an assignment is confirmed by a deletion in one position of the alignment. Often, such coils have a single inside assignment that serves as a hydrophobic anchor.

Step 8. Orient secondary structural elements with respect to each other

Secondary structural predictions based on the assignments described above typically identify better than 90% of the secondary structural units in a protein, with the residue-by-residue assignments of secondary structure correct over 70% of the time. The principal shortcomings of the method of the present invention are occasional misassignments of surface beta strands as surface coils (and vice versa), difficulties in assigning a conformation to strings near the active site (where functional conservation and adaptive variation often obscure patterns of conservation and variation that might otherwise indicate a particular secondary structure), difficulties defining precisely the beginnings and ends of the secondary structural elements, and difficulties distinguishing long internal bent beta strands from internal helices.

The first three difficulties do not as a rule obstruct the building of a supersecondary or tertiary structural model. Homologous proteins have similar, but not identical structures. Among homologous proteins, the beginnings and ends of secondary structural elements are not normally conserved during divergent evolution more precisely than ±1 amino acid. With the de novo prediction of protein kinase, the ends of the secondary structural units were predicted to ca. ±1.5 residues, perhaps the best that can be obtained using a method based on a comparison of homologous sequences Benner (1992) op. cit.!. Similarly, missing non-core structures does not normally obstruct the building of a supersecondary or tertiary structural model.

Only the last difficulty seriously hinders the assembly of a supersecondary or tertiary structure, as a mistake in assigning a core secondary structure almost always prevents the building of a tertiary structural model for a protein domain.

Assembling a supersecondary or tertiary structure is simplified if the secondary structural units form a recognizable supersecondary pattern, or fall into a particular taxonomical class of protein fold J. S. Richardson, "Anatomy and Taxonomy of Proteins", Adv. Prot. Chem. 34, 167-339 (1981)!. Further, if the protein contains disulfide bonds, or if chemical modification studies suggest that certain parts of the chain are close in three dimensions, this constrains the number of different types of folded structure that the protein can adopt.

However, the most general method for assembling a supersecondary or tertiary structure involves bringing the active site positions, scattered throughout the alignment, together in a way that is mechanistically plausible. This process also constrains the number of different types of folded structure that the protein can adopt. While each protein family is individual in this process, Example 1 shows how this can be done.

Step 9. Covariation analysis

Once a tentative model of the supersecondary or tertiary structure is in hand, it can be examined by looking for covariation, a pattern of conservation and variation that suggests that amino acid substitutions at distant parts of the alignment are not occurring independently. This in turn suggests that in the folded structure, these positions are not distant in space.

Scope of This Invention

For reasons that will be readily appreciated by those skilled in the art, the method of the present invention is most generally applicable to monomeric enzymes that are water soluble. The method is less satisfactory for enzymes that aggregate, or form multimers in solution, although predictions for dimeric and tetrameric enzymes are still quite reliable using this method. The method is still less applicable to enzymes that reside under physiological conditions bound within membranes.

EXAMPLES

Examples are provided concerning three proteins to illustrate how to make structure predictions.

The first, for protein kinase, is an example where an abundance of sequence information is available. This is the case where a secondary structure prediction was made before crystallographic information was available, and where a subsequently obtained crystal structure showed the prediction to be remarkably accurate.

The second, concerning pathogenesis related proteins from plants, considers a case where relatively little sequence information is available. At the present time, no crystallographic information is available for any member of this protein family.

Example 1 Predicting the Structure of Protein Kinase

The protein kinases form a large family of homologous proteins, with sequences of nearly 100 protein kinase catalytic domains available from work in many laboratories S. K. Hanks, A. M. Quinn, and T. Hunter, "The protein kinase family: Conserved features and deduced phylogeny of the catalytic domains", Science 241, 42-52 (1988)!. Thus, this example serves to illustrate the application of the method of the present invention to an extremely large alignment, the most preferred embodiment of the method of the present invention.

It is worth noting at this point that several groups have attempted to predict the folded structure of the catalytic domain of protein kinases using classical approaches, and that all of these predictions have been far from the mark. For example, a prediction by Shoji et al. S. Shoji, D. C. Parmelee, R. D. Wade, S. Kumar, L. H. Ericsson, K. A. Walsh, H. Neurath, G. L. Long, J. G. Demaille, E. H. Fischer, and K. Titani, "Complete amino acid sequence of the catalytic subunit of bovine cardiac muscle cyclic AMP-dependent protein kinase", Proc. Nat. Acad. Sci. 78, 848-851 (1981)! using a Chou-Fasman algorithm found three regions of the catalytic domain with different secondary structures, the first (positions 1-98 in the alignment discussed here) being highly (79%) helical, the second consisting of 3 "subdomains" (positions 99-146, 147-188, and 189-251) each consisting of a beta strand followed by two alpha helices and separated by two beta turns, and the third (252-end) being highly aperiodic (only 18% alpha helix and 20% beta strand). Other predictive work has focused on the fact that the amino terminal portion of the domain has the sequence GXGXXG, a sequence that is conserved in most members of the family. Such a sequence is also found in the "Rossmann fold", an α-β-α supersecondary structural unit that is present in several proteins that bind nucleotides and dinucleotides. Thus, several authors have suggested that this supersecondary structural unit is formed by the catalytic domains of protein kinases.

The typical protein kinase is expected to fold to give a globular structure, with a mean radius of ca. 26 Å. E. A. First, S. S. Taylor, "Selective modification of the catalytic subunit of cAMP-dependent protein kinase with sulfhydryl-specific fluorescent probes", Biochemistry 28, 3598-3605 (1989).!

Step 1: Obtain an alignment: An alignment of the sequences of the catalytic domains of a set of protein kinases was constructed Benner and Gerloff (1991) op. cit.!. The alignment is shown in Table 8, where individual protein sequences are read vertically downwards. The first column of numbers in Table 8 are the alignment numbers. These are used throughout this example to designate specific positions in the protein alignment. The second column of numbers contains the sequence number from mouse recombinant cAMP-dependent protein kinase. This is the enzyme for which a crystal structure was solved subsequent to this prediction. Further, the proteins in the alignment have been divided into 12 "functional subfamilies", sets of enzymes performing analogous functions, as indicated in the caption to the Table. Each column in Table 8 (including the gaps) has a "protein number" (from 1 through 90) which is used throughout the text to designate specific proteins.

Step 2: Divide Alignment into Subgroups: A matrix showing the pairwise identity of each sequence used here is shown in Table 9. The MPI values for the individual functional subfamilies is given in the caption to Table 9.

Steps 3-6: Assign parses, surface positions, interior positions, and active site positions. Table 10 shows, by alignment number, the surface, interior, hydrogen bonding variable, and parsing assignments made for the positions in this alignment.

Step 3: Divide the sequence into segments using parsing algorithms. Given the large number of sequences in the alignment, the first phase of the parsing can be executed using only the strongest parsing algorithm. Weaker parses are used when the individual segments are analyzed while assigning secondary structure.

It should be noted that in most of the proteins examined here, position 1 is not the first in a separate polypeptide chain, but is normally fused to a transmembrane region, from which it can be released by proteolysis. Such proteolysis often occurs in coils or loops, providing independent support for the canonical assignment. In the cAMP-dependent kinases specifically, the polypeptide chain begins 30-40 amino acids before the beginning of the alignment. It is conceivable that this leading segment adopts a standard secondary structure. If so, this structure is almost certainly broken by a distributed parse at positions -7 to -5 (where the alignment in Table 8 starts at position 1).

Primary Parse 1: 23-25-41-42. The first deletion parse is associated with a major insertion at positions 25-38. The parse is confirmed by parsing strings in some of the inserted sequences. For example, in protein 80, there is a PPNG sequence. The parse is reinforced at its amino end by P scattered in positions 23 and 24 in proteins where there is no insertion. It is reinforced at its carboxyl end by scattered P and deletions at positions 39-42. Further, alignment anchors at positions 44 (CM1 A) and 46 (APC K) make the alignment here indisputable.

Primary Parse 2: 49-50-59-61. There is a major insertion at positions 54-59. The parse is confirmed by parsing strings within several insertion. For example, protein 36 has a GGGGGGG string at positions 53-59. The region of disrupted secondary structure extends strongly on the amino end of the deletion. Scattered P's are found starting at position 49. Residues are deleted as early as position 49 (in protein 44), and a PP sequence is found at position 53-54 in proteins 70-72 (MPI=80%), and a PG at 50-51 (proteins 56-60, 63, MPI=70%). The parse cannot be extended on the carboxyl terminus. The single deletion in protein 29 at position 60 is probably a misalignment; an R can be moved from position 51. There are scattered P's in positions 60-65, but not sufficient to extend the parse in this direction. Alignment anchors at positions 47 and 67 (APC E) make the alignment here secure.

Primary Parse 3: 74-75-80-83. There is a major insertion at positions 75-80. The parse is confirmed by parsing strings within the insertion. For example, protein 17 has the sequence GGRGPGG at positions 74-80. The parse might be weakly extended on the amino terminus by a deletion at position 74 in proteins 52-54 which is not easily realigned, but no further. There are scattered P at positions 81 and 82, and a widely distributed P at position 83. The deletions in protein 14 at positions 81-83 are probably an alignment error; a KPP sequence can be moved down from positions 76-78 moving the deletion to these positions.

Primary Parse 4: 94-95-100-103. There is a major insertion at positions 96-100. The parse is confirmed by parsing strings within the insertion. For example, protein 52 has the sequence PAGSN at positions 96-100. The parse might be weakly extended on the amino terminus by a deletion in only one protein at position 94 (a deletion that cannot be accounted for by an alignment error), but no further. There are scattered P at positions 101-103; this segment is PP in two proteins (67 and 68, MPI=80%).

Primary Parse 5: 111-113-114-114. There is a 1-2 amino acid insertion at positions 113-114. The parse might be weakly extended on the amino terminus back to position 108, where the first P's are found. However, the P's do not appear to be significant before position 111. The parse cannot be extended on the carboxyl terminus. If the proteins containing the insertion are removed from the alignment, a secondary parse (a conserved GG string) would remain, thus confirming the parse.

Primary Parse 6: 123-126-128-132. There is a 3 amino acid insertion at positions 126-128. The parse is weakly confirmed by parsing strings within the insertion. For example, protein 36 has the sequence PPPG at positions 124-127. The parse might be extended on the amino terminus by a deletion at position 123, supported by P an G. However, as the preceding segment is assigned as a helix (vide infra), and a 3 amino acid deletion is found in proteins 39 and 40, this could indicate the loss of a single turn of a helix. There are scattered P at positions 129-132; the distribution does not make a strong case for an extension of the parse, however.

Primary Parse 7: 167-170-176-176. There is a major insertion at positions 170-176. The parse is only weakly confirmed, however, by structure disrupters within the insertion (a lone P in protein 81 at position 173). The parse might be weakly extended on the amino terminus by scattered P at positions 167-169. The sequences NG (proteins 5-8), PG (protein 26) and a deletion (protein 76) at position 169 make this extension at least plausible for a single position. However, the parsing element cannot be extended on the carboxyl side.

Primary Parse 8: 190-194-197-200. There is a 2 to 4 amino acid insertion at positions 194-197. The parse is weakly confirmed by P's scattered within the insertion, including a weak distributed parse in functional group 7 (MPI=35%) (positions 196-197). The parse might be extended on the amino terminus by a scattered P's back to position 190. Further, P's are scattered throughout the next segment leading to Primary Parse 9. Especially notable is a strong secondary parse at position 209, a conserved P. A PP sequence is found in proteins 46, 65, 80, and 84 (MPI <35%). Thus, it is difficult to terminate this parsing region at the carboxyl end.

Primary Parse 9: 213-213-216-220. There is a major insertion at positions 213-216. The parse is not confirmed, however, by parsing strings within the insertion. The parse cannot be extended on the amino terminus. Thus, it is appropriate to examine the alignment in this region. The APC E (position 210) and CM3 P (position 209) firmly anchor the alignment on the amino end. The APC E is normally an indicator of an active site residue, as is the presence of conserved strings 4 positions (and longer) in length in subgroups with MPI values below 50%. The alignment is solidly anchored on the carboxyl side of this parse by positions 227, 228, and 229. There is, a highly conserved P at position 209 which may indicate a secondary parse (see above). Scattered P's are found at position 218 and 220; none are found at 217 or 219. Thus, it is difficult to extend the parse in the carboxyl direction.

Primary Parse 10: 223-224-224-225. There is a 1 amino acid insertion at position 224 in proteins 28-31(MPI=50%). However, if the sequences of proteins 28-31 is shifted up by one position in the alignment between positions 217-224, the gap disappears. In its place remains a distributed parse extending from position 218-225 in the serine protein kinases (functional groups 1-7), but not in the tyrosine protein kinases. This parsing element is strong. A single deletion is found in protein 36 at position 220.

Primary Parse 11: 241-241-241-241. There is an insertion of a single amino acid at position 241. The parse is not confirmed by parsing strings in this insertion, nor is it confirmed on either side. The insertion might be an alignment problem; shifting the sequences of proteins 1-54 up by one position would move this deletion down to the major deletion that follows. However, the two plausible alignment anchors at positions 245 and 246 would be disrupted by this shift.

Primary Parse 12: 244-249-259-263. There is a major insertion at positions 249-259. The parse is confirmed by structure disrupters within the insertion. For example, protein 44 has the sequence PRGP at positions 250-253. The parse can be extended on the amino terminus by a deletion at positions 247-248, and scattered P back to position 244, with a highly conserved P at position 245. Widely scattered P's are present at positions 260-263, although the fact that the following segment is possibly an alpha helix could indicate that this segment involves the presence or absence of a single turn of a helix.

Primary Parse 13: 272-280-285-289. There is a single amino acid insertion at position 273. The parse is confirmed by 2 P's in the insertion. There are also 5 P's at position 272, and protein 36 has a PP sequence in this segment. On the carboxyl end, P's are scattered all of the way until position 276. Thus, it is difficult to end the parse. Position 275 may be a hydrophobic anchor for an external loop. Further, and most seriously, the parse might be collapsed by shifting the residues in functional subfamilies 1-4 up by one position. For this discussion, the alignment has therefore been realigned to remove the deletion at position 273.

There is a major insertion at positions 280-285. The parse is confirmed by scattered P's within the insertion. For example, protein 26 has the sequence PWPS at positions 281-284. The parse is almost certainly extended on the amino terminus by a deletions up to position 276; these cannot be accounted for by a misalignment. Further, P's are densely scattered back to position 276. Finally, disrupting sequences can be found in this region; a PPP is found in protein 42 at positions 276-278. On the carboxyl end, scattered P's are found from positions 286-289. However, the fact that the following segment is possibly an α helix could indicate that this segment involves the presence or absence of a single turn of a helix.

Primary Parse 14: 314-314-318-320. There is a major insertion at positions 314-318. The parse is only weakly confirmed by parsing strings within the insertion. The parse cannot be extended on the amino terminus. However, a few P's at position 320 might weakly extend the parse to this position.

It is worth noting that the end of this alignment does not correspond to the end of many of these proteins. It is, however, the end of the alignable catalytic domains. Thus, the end of the protein is not necessarily a strong parsing element in these cases. In functional subgroups 2, the sequence extends 12 positions before an apparent parse. An apparent parse is found in functional group 8 only two positions past the end of the alignment.

The regions of the alignment included in the deletion parses are assigned as surface coils, loops, and turns. Such structures are strongly indicated for the alignment numbers indicated in bold face. The extent of these structures is determined in part by the secondary structures assigned to the parsed segments that lie between them. These are considered below.

Step 4: Assign surface positions: Surface positions are identified by the presence of variation in more than one subgroup at different levels of minimum pairwise identities. These are tabulated in Table 10. The column in which an entry is made indicates the number of variable subgroups in a particular cluster of subgroups with a particular MPI, with the MPI of the subgroups designated by the first number in the entry, followed by a period and a number indicating the number of these subgroups that contain at least one polar residue (D, E, K, N, R). Due to the large number of aligned sequences, the assignments should be quite accurate (>95%) and relatively complete (>95% of all surface residues identified). On helix wheels, surface positions are followed by x.y.z, where x is the number of variable subgroups in cluster y, with z the number of subgroups with a polar residue (D, E, K, N, R).

Step 5: Assign interior positions: Interior positions assigned using the algorithms given above are listed in Table 10. Upper case INSIDE assignments are reserved for the more reliable assignments, while lower case Inside" assignments indicate less reliable assignments. On helix wheels, inside positions are indicated boldface.

Step 6: Assign active site positions: Active sites are assigned to every position in the alignment where a functionalized side chain (C,D,E,H,K,N,Q,R,S,T) is conserved across the entire alignment. These are listed in Table 10. Conserved strings are discussed in the appropriate segments.

These assignments can be combined with experimental work that uses chemical modification to identify regions of the protein that are involved in binding ATP and protein substrates. This experimental information is often redundant. However, as certain assignments of active site residues (in particular, assignments to the active site of highly conserved charged residues) are not highly reliable, this extra information is valuable to confirm active site assignments made by our approach.

Step 7: Assign secondary structure. These data are used to construct secondary structural hypotheses for parsed segments of the alignment. A complete secondary structural assignment is presented in Table 11. The segments are designated by the alignment position numbers that they encompass. As noted above, the parses comprise different segments in different branches of the evolutionary tree. Normally, the ends of secondary structural units are expected to be different by one or two residues in different proteins. Therefore, the segments are designated by position number, with the bold face numbers inside the minimum extent of the segment, and the outside number (plain type) the greatest extension of the segment. This is the origin of the maximum and minimum lengths reported in Table 11. The preferred length is tailored to the cAMP-dependent subfamily, as this is the subfamily presently being studied crystallographically. Normally, parses are included as the last position of a helix unless the parse is a deletion.

This variation in lengths presumably represents the fact that the structures of homologous proteins whose sequences have diverged by over 70%, although similar overall, are different in detail. This is, of course, a limitation on any method that builds models from a set of homologous proteins.

Detailed discussions for each of the 15 segments of the alignment created by the primary parses are given below. Helix wheels for these segments can be found in FIG. 3.

Segments 1 and 2. This segment is longer than might be expected for a single secondary structure unit. Further, the second part is largely hydrophobic, the first part largely hydrophilic, and no pattern of amphiphilicity can be found to indicate that it forms a helix. A secondary parse is therefore sought to break the segment into two or more shorter segments. The APC G at position 12 and the CM1 G at position 10 are individually secondary parse; together they make a strong secondary parse. This suggests a break at positions 10 or 12. There are some strings in individual protein sequences that confirm a parse in this region. For example, proteins 67-69 conserve (in a subgroup with an MPI=70%) a GGG string at positions 10-12. GG strings in position 12-13 are found in proteins 9, 46, and 52-55. Further, in protein 44, the T at position 10 that substitutes for an otherwise conserved G is complemented by a P at position 11. Thus, the first segment is parsed into two smaller segments, positions 1-10 (Segment 1) and 12-24 (Segment 2), and the secondary structure of these segments considered separately.

The first segment contains both surface and inside assignments. A helix wheel shows no obvious amphiphilicity across the entire segment. An amphiphilic helix can be built from position 1-14 only if position 8 is regarded as a surface (K=5, 6 variable subgroups, 5 with polar residues), the surface assignment at position 2 is ignored (perhaps justified by the generalization that positions "inside" but at the ends of an amphiphilic helix often lie on the surface), and the surface assignments at positions 5 and 6 are treated as weak. These ad hoc assumptions are weak. For example, the surface assignments for position 6 (13 variable subgroups, 4 with polar residues) and position 11 (10 variable subgroups, 5 with polar residues) are of essentially identical strength. Yet in the amphiphilic helix, position 6 is in the middle of the "interior" region, while position 8 is in the middle of the "surface" face of the helical projection. On these grounds, the assignment as a helix is rejected.

The remaining two possibilities are as a surface coil or as a end beta. As noted above, the method of the present invention often cannot distinguish between these two structures, although the distinction is not particularly critical, as end beta strands do not form the core of a tertiary structure. The scattered P's at positions 5 and 7 and the consecutive surface assignments at positions 4-8 favor assignment as a coil.

The surface assignments in the second segment are weak, especially compared with those that are made elsewhere with such a large alignment. It has a low fraction of surface assignments, a large number of splits, no pattern of helical amphiphilicity, and APC residues (or nearly APC residues) at positions 12, 15, and 17. Thus, the segment from positions 12-20 is treated therefore as an internal segment, and it must be decided whether it is an internal beta strand or an internal helix. With only 9 amino acids (one being a parse), the segment length indicates a beta assignment. Looking to the ends of the strand, one finds alternating patterns of periodicity. In particular, the strongest surface assignments are at positions 19, 21, and 23, an alternating pattern that indicates canonically that this is a beta structure. Essentially no evidence suggests that this segment is an internal helix.

At its greatest extent, the beta strand might begin at position 10, and end at position 23 (14 amino acids). This beta strand is longer than typically found in folded proteins. The strand can be shortened by removing the parsing positions at the beginning of the segment (certainly to position 12, possibly to position 15). Alternatively, the beta strand can be bent at position 15-16 (secondary parse) to form two shorter beta strands, the first 4 positions in length, the second 7.

We have no grounds for preferring a start of the strand at position 12 or 13. The choice of the end point at position 22 is based on the alternating pattern observed in some subgroups of the alignment, and the need to have a sufficient number of filled positions in functional subfamily 7 to execute a standard turn before the next secondary structural segment (vide infra).

This secondary assignment, classed as "moderately reliable", is controversial. This segment contains the string GXGXXG, well-known in proteins that bind ATP and adenine dinucleotides R. K. Wierenga and W. G. J. Hol, "Predicted nucleotide-binding properties of p21 protein and its cancer-associated variant", Nature 302, 842-844 (1983).!. Thus, several investigators, using classical methods, predicted that this segment indicates that the catalytic domain of protein kinases is homologous to other kinases and, in particular, this first region is a strand-turn-helix structure. A particularly convincing case for this is made by Sternberg and Taylor, who compared this region with the NAD+ binding regions of glyceraldehyde-3-phosphate, lactate and alcohol dehydrogenase, and the FAD binding regions of glutathione reductase and p-hydroxybenzoate hydroxylase M. J. E. Sternberg and W. R. Taylor, "Modelling the ATP-binding site of oncogene products, the epidermal growth factor receptor and related proteins", FEBS Lett. 175, 387-392 (1984).!. In these proteins, the GXGXXG sequence lies between a beta strand and an alpha helix (a beta-turn-alpha structure), and Sternberg and Taylor concluded that this region had a similar conformation in the protein kinases discussed here. Thus, the prediction made by classical methods is different from the prediction made by the method of the present invention.

There are no assignments of active site residues in this segment. The absence of active site assignments in this region contradicts an experimental fact, that Lys 7 is protected from modification by acetic anhydride in the presence of Mg-ATP and an inhibitory peptide J. A. Buechler, T. A. Vedvick, S. S. Taylor, "Differential labeling of the catalytic subunit of cAMP-dependent protein kinase with acetic anhydride: Substrate-induced conformational changes", Biochemistry 28, 3018-3024 (1989)!, which may indicate that this segment is near the active site.

Segment 3. The proteins of functional subfamily 7 have a gap in the alignment that extends fully from position 25 to position 41. This fact implies that positions 24 and 42 lie close together in space in the other proteins, suggesting that if the beta strands assigned to positions 12-22 and positions 42-48 are part of the same beta sheet, they lie antiparallel. Further, it leads us to terminate the preceding beta strand one residue sooner and start the following beta strand one position later than would be done based on analysis of the first segment alone. There is no evidence for active site residues in the coil.

Segment 4. The dominant feature of this segment are the interior assignments to positions 43 to 46 and 48. Further, there are several hydrophobic splits throughout the alignment, indicating an ordered structure (rather than a coil). This is an internal segment (43-46); from its length, the canonical assignment for this segment is a beta strand. This assignment is confirmed by the alternating pattern at both ends, and is strongly confirmed within the strand by the two-position periodicity in the pattern of conservation and variation, especially evident in subgroups of the alignment with MPI values near 60%. For example, functional group 11 (MPI=50%) has the string 43 (V or C); 44 (conserved A); 45 (I or V); 46 (conserved K); 47 (surface); 48 (V or L); 49 (surface). Thus, this secondary assignment is classed as "very strong".

The most significant evidence for a 3.6 residue periodicity that might suggest an internal helix is the 3 amino acid deletion at the beginning of the segment (positions 39-41) in functional subfamily 7, and the deletion of three amino acids at positions 49-52 with respect to protein 29, and four amino acids (49-53) in protein 44. The strong alignment anchors at positions 44 and 46 fix this deletion. However, these deletions are also consistent with a coil structure at the ends of this segment, and no other evidence appears to suggest a helical assignment for this region.

Position 46 (APC K) is strongly assigned to the active site. This assignment is consistent with experimental data. Lys 46 is modified by fluorosulfonylbenzoyladenine, a substrate analog. Further, when cAMP-dependent protein kinase is treated with dicyclohexylcarbodiimide, it appears that a major product is an intrachain crosslink with Asp 182. Both residues are absolutely conserved, and the modification is blocked by the presence of Mg-ATP M. J. Zoller, N. C. Nelson, S. S. Taylor, "Affinity labeling of cAMP-dependent protein kinase with p-fluorosulfonylbenzoyl adenosine", J. Biol. Chem. 256, 10837-10842 (1981)!.

Segment 5. This segment contains both deletions and parsing elements, and therefore is assigned a coil structure. Protein 44 has the entire region from position 49-59 deleted, implying that positions 48 and 60 lie close together in space in the other proteins. Functional subfamily 8 also deletes much of this region, with parsing indicators present as late as position 63. Functional subfamily 10 (MPI=65%) shows covariation between positions 50 (EEK) and 55 (KKE), consistent with an interaction between these two residues in this subgroup of proteins. The parse in this region has some noteworthy features. Protein 36 has a string of 7 consecutive glycines (positions 53-59). Also, there are 3 consecutive D's in functional subfamily 2 (positions 56-60). The scattered P's at positions 60-63 suggest that the parse might extend as far as position 63. There is no evidence for active site residues in the coil. However, the segment 52-57 could adopt a standard secondary structure in functional subfamilies 1 and 2; a helix is consistent with the sequences in these proteins.

Segment 6. The alignment is well-anchored in this region by the APC E at position 67. On the amino end, the alignment is secure at position 46 (APC K).

From position 64 to 73, the interior and surface assignments map well on a helical wheel (FIG. 3a). Therefore, this segment is canonically assigned as an a helix. This assignment is initially classed "moderately strong", as it involves relatively weak surface assignments. Indeed, the strongest surface assignment based on variable subgroups is at position 65 (9 variable subgroups, K=4). However, only 3 of the variable subgroups at this position have a polar residue (DEKRN). Indeed, the amino acids found at position 65 are largely hydrophobic, and it is also worthy of note that proteins 46 and 61 have P at this position. Position 66 has the largest number of variable subgroups (8) where the variable subgroup also includes a polar residue (7). Position 64 is a functional variable (7 variable subgroups), a strong indicator of an inside position in an alignment this large. This position is also listed as a hydrophobic variable (4 variable subgroups, K=5). Position 65 is also listed as a hydrophobic variable, 2 variable subgroups (K=9,8). The rather weak assignments of positions to the surface suggest that this helix is partly buried, especially when compared with other helices (e.g. the helical conformation assigned to segment 14.

To strengthen (or possibly weaken) the assignment, other indicators are examined. Covariation analysis identifies a few interesting residues that are consistent with a helical assignment in this position. For example, in the three proteins in functional subfamily 10, the residues at position 62 are KKE, while the residues at position 66 are DDR. This pattern of covariation is consistent with an intrahelical contact between the side chains at these positions in these proteins, and provides further support for extending this helix to position 61 in at least some of the functional subfamilies.

The first four positions of this segment (positions 60-63) are assigned to the surface, canonically indicating a coil. Scattered P's in this segment (e.g., conserved in proteins 33-36, MPI=50%, 56-59, MPI=80%) and the parsing string PXP (protein 62) confirm this assignment as a coil. However, a dialectic position is possible that the helix extends another full turn, starting from position 60. In this extra turn, position 63 falls on the inside of the helical projection. As this position lies at the end of the helix, this assignment is not entirely incompatible with the moderate surface assignment at this position (Table 10). However, it is conceivable that the first turn of the helix is missing in proteins in functional subfamily 8 (for example), where the preceding coil is short.

In the preferred assignment (Table 11), the last position is position 74 due to the inside assignment. The choice of position 61 as the first position of the helix is somewhat arbitrary; it was made because the cAMP-dependent protein kinases have a hydrophobic residue at this position, and the parses at positions 60 and 75 match.

Especially noteworthy about this segment is the absolutely conserved E at position 67. This is a glutamate in the middle of the hydrophobic region. Canonically, one assigns an APC E as an active site residue, which would place this helix at the active site. There are, of course, other roles for amino acids with functionalized side chains that are sufficiently important that the residue at that position is highly conserved. For example, in lactate dehydrogenase, an APC E (311) is found in the middle of the final helix, where it forms a hydrogen bond to W 203 (which is also conserved). Likewise, an APC E in alcohol dehydrogenase is found at position 35 in a beta strand not at the active site. In the putative helix in the protein kinase family, there is little to indicate that this segment is at the active site; for example, there is no string of conserved residues at low MPI (compare, for example, the pattern of conservation in the active site helix between positions 46 and 55 in the ADH family). Thus, for future assembly of the secondary structural elements into a folded structure, this helix need not end up at the active site.

In this context, several experimental facts are valuable. First, Glu 67 is especially reactive with carbodiimides, implying that it lies in a hydrophobic region, and the reactivity is diminished in the presence of Mg-ATP ( J. A. Buechler, S. S. Taylor, "Identification of aspartate-184 as an essential residue in the catalytic subunit of cAMP-dependent protein kinase", Biochemistry 27, 7356-7361 (1988)!, implying that it lies near the active site. Further, the lysine at position 68 appears to be inside the protein fold based on its slow reaction with acetic anhydride.

Segment 7. In functional subfamily 7, all amino acids are deleted from position 74 to 80, indicating that positions 73 and 81 are close in space in the remaining proteins. There is no evidence for active site residues in the coil.

Segment 8 This is the first of several beta-like segments that are broken by secondary parsing segments at several places throughout. Only two strong surface assignments are made after position 84 in this segment, at positions 87 and 94. A third, at position 90, is weak enough to be indecisive. The remaining positions are hydrophobic splits (e.g., at positions 86 and 90, the splits are perfect at MPI=70%). Thus, the canonical assignment for this segment is a beta strand from position 84 to position 93. However, the assignment is classified only as "strong" because it is difficult to confirm this assignment by alternating patterns in the alignment.

Central to this difficulty is the fact that prolines are found scattered throughout this segment, at position 87, and at position 91. These secondary parses are potentially valuable as the beta strand is long, and is probably bent. Position 87 is interesting in this regard. Positions displaying variability of this sort are often found in beta strands that emerge above the surface of the globular protein at a point where the beta strand bends and then continues, in this case until position 93 or 94. The fact that isolated P's are found in proteins 40, 44, 50, and 57 at position 87 supports this picture, as does covariation analysis with the following segment (vide infra). It is worth noting that the lysines at position 87 and 93 appear to be inside the protein fold based on reaction with acetic anhydride J. A. Buechler, S. S. Taylor, "Differential labeling of the catalytic subunit of cAMP-dependent protein kinase with a water-soluble carbodiimide: Identification of carboxyl groups protected by Mg-ATP and inhibitor peptides", Biochemistry 29, 1937-1943 (1990)!. Finally, the carboxyl side chains of E 89 is especially reactive with carbodiimide, and is not protected by substrate, suggesting that it is inside the folded structure but not at the active site.

The parse at the amino end extends strongly to position 83, suggesting that any beta-like structure begins only at position 84. The segment is ended at position 93 in the cAMP-dependent protein kinases (Table 11) because of the deletion in protein 39. The two amino acid deletion could, however, indicate that in the remaining proteins, the beta strand continues two positions further.

Dialectically, one can attempt to assign this segment a helical structure. Positions 84, 87, and 94 can be assigned to the surface with varying degrees of reliability. These map on one side of a helical wheel. Position 90 maps extremely weakly on the surface, and appears on the correct side of the helix. Position 91 violates the amphiphilic helical pattern. Based on the assignments at positions 90 and 91, it is unlikely that this segment is a surface helix; however, a helical assignment might be made if the assignments at positions 90 and 91 were in error. Therefore, the possibility that it is an internal helix, or a helix forming a contact with a membrane or another protein subsegment cannot be ruled out.

However, "functional variable" assignments at positions 85, 86, 91, and 92 are distributed evenly around the helical projection, and offer no support for a helical assignment. Further, none of the 12 helical wheels for the functional subfamilies show convincing patterns of amphiphilicity, either in the pattern of variation or in the polarity of the amino acid residues themselves, largely because position 86 remains firmly inside. Thus, a helical assignment is rejected.

Segment 9. Positions 94-100 contain no amino acids in protein 39, suggesting that positions 93 and 99 are close in space in the remaining proteins. However, protein 39 is among the most structurally divergent in from the bulk of the alignment, and a more reliable hypothesis is that positions 95 and 99 are close together in the folded structures. This implies that if the preceding and following beta strands belong to the same beta sheet, they reside antiparallel. Covariation analysis (vide infra) suggests that this is the case. There is no evidence for active site residues in the coil.

Segment 10. This segment appears to be another long broken beta strand. The alignment is anchored in this region at position 108. Especially unusual is the 2 residue deletion (positions 108 and 109) in protein 5, which is 90% sequence identical with proteins 6 and 8. This deletion corresponds to a single P in functional group 11 (protein 85), and two P's in functional group 6 in proteins 40 and 44. Position 108 is unusual; it is a highly conserved E in the middle of a hydrophobic stretch. This deletion can be moved down in the alignment by realigning the segment; the alignment anchors in this region are not decisive, and this realignment is the basis for the analysis that follows.

The segment forms part of the interior core of the protein, with positions 103-110 all assigned to the inside (position 105 has 5 variable subgroups, K=4, with a single variable subgroup with a polar residue). This is canonically assigned as a beta strand.

Again, the beta strand is rather long, and probably bent at position 108. This notion has an interesting consequence in the context of the assignment of Segment 8. There is substantial covariation between position 87, which has a high proportion of basic residues, and position 108, which has a high proportion of acidic residues. In particular, in proteins 40, 44 and 85, the E at position 108 is replaced by a P, and in all three cases, the basic residue at position 87 is replaced either by a P (proteins 40 and 44) or by an S (protein 85). The covariation is striking in functional group 7, where position 87 has LLPLRRR matched against QQQQEEE at position 108. Thus, it seems to be a reasonable working hypothesis that these beta strands are in close proximity, with the bend at position 87 matched to the break at position 108, with a contact formed between the side chains of the amino acids at these two positions.

Covariation analysis is one of the most problematic ways of finding contacts between distant points in a polypeptide chain. We do not yet have programs to do covariation analysis on an alignment as large as protein kinase. Further, covariation is rarely perfect. However, in the absence of disulfide bonds, it is the only way to constrain the distance between portions of the chain that do not lie at the active site. In any case, it seems certain that covariation analysis is most useful when one already has a structural hypothesis, as is the case here.

Segment 11. This coil has little variability in length, with 2-4 positions. There is some experimental evidence that this coil lies near the active site. When the phosphorylatable serine in the peptide LRRASLG is replaced by the photoaffinity agent, p-benzoylphenylalanine, Gly 113 and Met 116 are modified W. T. Miller, E. T. Kaiser, "Probing the peptide binding site of the cAMP-dependent protein kinase by using a peptide-based photoaffinity label", Proc. Nat. Acad. Sci., 85, 5429-5433 (1988)!.

Segment 12. Positions 115-123 of this segment show a 3.6 residue periodicity indicative of a helix (FIG. 3b). However, the assignment classified only as "strong". A plot of the surface/interior assignments on a helical wheel shows only weak amphiphilicity. Positions 115, 117 and 119 are assigned only weakly to the surface (only 5 variable subgroups K=4). The strongest surface assignment is at position 118, and at 122, but here the amino acid is at the end of a secondary structural element (given the parse that follows), making it unlikely that a surface assignment here would be definitive in ruling in or out a particular assignment.

As shown below, there is reason to believe that the serine and tyrosine kinases are not perfectly aligned in this region. A misalignment has two implications. First, it is difficult to identify secondary structure from an examination of the entire alignment. Second, examining fragments of the alignment should be a more productive way to assign secondary structure.

The segment is flanked on both sides by deletion/insertions of varying lengths. Second, there are no anchors for the alignment within the segment. Indeed, there are essentially no anchors for some distance outside the segment. The Gly at position 113 might be considered as an alignment anchor, but it comes in a loop region. The substitutions at position 106 provide a weak anchor, as might the scattered P in the two main classes of kinases at position 132. However, on the amino end of the segment, the last completely solid anchor is at position 67, although weaker anchors might be found at position 108 (where the ancestral residue is presumably E) and perhaps the hydrophobic element at positions 103-107 (although alignments based on such an orientation can be plus or minus a single residue). The next completely solid anchor on the carboxyl side comes at 155-157 to the carboxyl side, although weaker anchors exist at positions 144, 140 (where Q might be reconstructed as the primitive residue in the common ancestral sequence), and 139 (with A as the common ancestor). Clearly, in between, the alignment is unreliable.

However, when the segment is divided into the 12 functional classes and the data replotted, the helical ambiguity goes away. For example, functional subfamily 3 at position 119 has either the hydrophilic D (proteins 20 and 21) or R (proteins 24, 25, and 26), while the position is the hydrophobic Y in proteins 22 and 23. The distribution is tree-like based on the overall sequence identities of the proteins. The situation is mirrored on the other side of the putative helix. Position 121 has either the hydrophobic V (proteins 20, 21, and 24) or I (proteins 25 and 26), while the position is the more hydrophilic T in proteins 22 and 23. Thus, it appears that in proteins 22 and 23, the helix is rotated a bit "clockwise" with respect to the folded structure of the protein. Table 12 lists the borders of the helix in the different functional subfamilies.

This region illustrates also how information appears at different levels of sequence divergence. Group 2 does not obviously show an amphiphilic helix, and a helical assignment of this segment would not be secure if these were the only sequences available to the biological chemist.

When a helix has become "reoriented" by a residue (a statement that, at the present level of analysis, is equivalent to a statement that the alignment is misconstructed due to a shift of one position), the assignments made across the shift become confused, and assignment of secondary structural elements becomes weaker. However, if a helical pattern is strengthened by assignments based on a partitioning the alignment to avoid including shifted (or misaligned) structures within a single alignment, a "very strong" assignment of secondary structure can be obtained.

For the cAMP-dependent kinases, the preferred helix extends from position 115 to position 124. The fact that position 123, assigned to the surface, appears on the inside face of the helix is acceptable as it is at the end of the helix. The last turn of the helix is probably missing in members of functional subfamily 6.

Segment 13. In proteins 39 and 40, positions 123-128 are deleted, suggesting that positions 127 and 129 are close in space in the remaining proteins. Further, this suggests that the parse extends until position 132, although it can be argued (vide infra) that the following helix begins as early as position 131. There is no evidence for active site residues in the coil.

Segments 14-17. This is the longest unparsed segment in the alignment (41 positions with no insertions or deletions). A helix of this length would have 11 turns, and be approximately 60 Å in length; a single beta strand would, of course, be longer. 60 Å is considerably longer than the expected diameter of a spherical globular protein of this length, implying that there should be an internal parse. The first four positions (129-132) are plausibly coil structures (note the parsing string PXP conserved in part of functional group 1, MPI=80%). A secondary parse is present at position 160. These potentially divide or shorten standard secondary structures that might be assigned to this segment.

However, the most striking features of this segment are the highly conserved strings RDLK and RDLA at positions 155-157. Such strings are assigned canonically to the active site, and are often found in loops or coils, making plausible a break in a helical structure up to this point. Further, the reaction of E 161 with water-soluble carbodiimide is partially by Mg-ATP, and fully blocked in the presence of both Mg-ATP and peptide substrate J. A. Buechler, S. S. Taylor, "Identification of the peptide recognition site in the catalytic subunit of cAMP-dependent protein kinase", J. Cell Biol. 107, 491a (1989)!, experimental evidence favoring an active site assignment for this segment.

The segment has the most distinctive alpha helical pattern in the protein, with amphiphilicity extending in a "textbook" fashion from position 131 until 151, over 5 turns (FIG. 3c). Textbook surface helices of this length are essentially never misassigned, making this among the strongest secondary assignments in this alignment.

It is worth a few words to examine the extent to which this helix is confirmed by the details of the pattern of conservation and variation. Position 135 at the boundary between the surface and inside faces of the helical projection is rather weakly assigned to the surface. Although there are 10 variable subgroups at MPI=60%, only 2 contain a polar amino acid. Closer inspection of the alignment shows that the surface assignment comes from subgroups in the serine/threonine kinase functional class; the sequences of the tyrosine kinases alone would yield an interior assignment.

It then remains to determine how far this helix can be extended in either direction. The amphiphilic pattern is disrupted in the amino direction by the surface assignment at position 130 (which is on the inside face of the helix) and by the inside assignment at position 129 (which is on the surface face of the alignment. Position 130 may still be a part of the helix, of course; as noted previously, "inside" amino acids at the end of amphiphilic helices are often found on the surface. However, this implies that the helix does not extend more than a half turn further in the amino direction. This notion is confirmed by the scattered P's at position 132. Again, P can be in a helix, but normally within one turn of the amino end. Based on this reasoning, the amino terminus of the helix is designated as position 131.

The carboxyl end of the helix is more difficult to assign. Position 149 technically breaks the amphiphilic pattern of the helix, but with only 3 variable subgroups (MPI=50%), and only 2 of these having polar residues, this is a weak surface assignment and is not decisive. Positions 150 and 151 are assigned to the surface and appear correctly on the helical wheel. Position 152 is a surface assignment appearing on the "inside face" of the projection, 153 an "inside" assignment on the inside face, and position 154 an inside assignment on the surface face of the helix. These positions mark the end of the amphiphilicity in the helix.

Attempts to extend the helix further are problematic, as the segment now becomes highly conserved and highly functionalized. Consecutive conserved functional residues are strongly assigned to the active site, and functional constraints and adaptive variation often obscure patterns that would otherwise be good indicators of secondary structure.

The helix is rather long (22 amino acids, a bit over 6 turns), with a total length of ca. 33 Å. Thus, it traverses the entire globular structure of the protein, and it is difficult to imagine the active site perched on the end should we extend the helix much farther than position 153. The implication is that the helix must end here, that the parse at position 160 must be a real break in secondary structure, and that the secondary structure of the segment that follows (from position 161-169) must be considered separately.

The remainder of the segment is divided into three segments. The first reflects the possibility that there is a short beta strand at the end of the helix (positions 153-156). This canonical assignment is based on the large number of splits. Unfortunately, this assignment is classified as "weak" because splits near an active site are also indicative of coil structures.

The next subsegment (segment 16) is clearly an active site coil based on the APC D at position 157 and the APC N at position 162.

The following subsegment (segment 17), a string (positions 163-165) of interior assignments, is canonically assigned as a beta strand.

The lysine at position 159 appears to be inside the protein fold based on reaction with acetic anhydride. Further, the carboxyl side chains of E 161 is especially reactive with water-soluble carbodiimide; it is completely protected by Mg-ATP and an inhibitory peptide. This confirms the assignment of this region to the active site Buechler & Taylor, 1988, op. cit.!.

Segment 18. The four consecutive surface positions (166-169) at the end of the previous segment are canonically assigned as a coil. As the deletion in protein 76 starts at position 169, and the alignment is well-anchored in this region, the minimum length of the parsing segment is 2 amino acids, and position 168 is likely to be near position 177 in the remaining proteins. There is no evidence for active site residues in the coil.

Segments 19 and 20. Problems arise because within this segment, differences in the sequences of the different subgroups of the kinases make the alignment difficult to construct reliably. However, these differences cannot be explained as the random variation expected for a segment that lies far from the active site as their is an absolutely conserved triplet DFG at positions 182-184. The conserved triplet is an extremely strong indicator of a segment near the active site.

It is worth noting here is that it is the conserved string of three residues that makes this assignment strong. APC D is known at positions other than the active site, and APC G is often simply a parse. APC F is occasionally found in the center of hydrophobic cores. Thus, each conserved amino acid alone would not be a strong indicator that this segment lies near the active site. Further, nearby positions display reflexivity. For example, at position 186, functional subfamilies 1-7 have A, S, and C, while functional subfamilies 8-12 have A, S, and T. Such reflexivity often indicates a purely structural constraint on divergence. However, a conserved string such as the one present here is essentially never found far from the active site.

Chemical modification studies also suggest that this segment is at the active site. Treatment of a cyclic AMP-dependent protein kinase with carbodiimide yields a protein crosslinked between the side chains of Asp 182 and Lys 46 J. A. Buechler, S. S. Taylor, "Dicyclohexylcarbodiimide cross-links two conserved residues, Asp- 184 and Lys-72, at the active site of the catalytic subunit of cAMP-dependent protein kinase", Biochemistry 28, 2065-2070 (1989)!. Mg-ATP blocks this reaction. Chemical modification studies support the notion that K 46 lies at the active site, implying that the pair is at the active site.

Extreme variation in sequence near an active site is, of course, anticipated for a set of homologous proteins whose function has undergone divergent evolution. This is a strength of the approach used here, as neither variation nor hydrophilicity is automatically assigned to the surface, but rather only specific types of variation and hydrophilicity. Thus, the variation in this region is interpreted as evidence that this segment forms a structure at the active site that is important for binding the protein substrate. As the structure of the natural protein substrate is quite different for different functional subfamilies, one expects both the sequence and the folded structure of this region to be different in different proteins, meaning that the assumption central to our approach is probably not entirely true in this region of the alignment.

Canonically, a string of inside residues and splits (e.g. positions 177-186; note that the surface assignment at position 177 is weak) is assigned a beta strand structure. Here, it seems plausible to associate the strand at position 182 with the beta strand assigned between positions 42 and 48, primarily on experimental data obtained by crosslinking experiments. As discussed below, these two beta strands appear to be aligned antiparallel to each other. Further, if the first beta strand is bent at position 46, so would the second (at position 182).

Positions 190-193 are assigned to the surface, position 90 strongly in all subgroups of kinases. Four consecutive surface assignments are canonically assigned as a coil. In functional subfamilies 1-6, these positions also contain scattered P's throughout. Thus, the canonical assignment of this subsegment is a coil in this subfamily. However, in other functional subfamilies, other secondary structural assignments are possible, especially as no P's are found at this position in functional subfamilies 7-12. It is important to look closely at these, starting with the functional subfamily with the lowest MPI, as this is the subgroup that retains the most information, provided that the tree within is appropriately branched.

Functional subfamily 7 has a MPI=35%, and is divided into two subgroups (proteins 48-51 and 52-54 respectively) with MPI=80%. Thus, the extent and distribution of diversity of the proteins in functional subfamily 7 is nearly satisfactory for this functional subfamily to serve (by itself) as the basis for an application of our structural prediction method. Indeed, did we not have the other sequences, we would attempt to assign a structure from these 7 proteins alone, although the reliability of the structural assignments would, of course, be much lower than the ones presented here.

The segment in functional subfamily 7 between positions 184 (APC G, viewed as a parse for this discussion) and 191 maps as an amphiphilic helix. At positions 188 and 189, the apparently contradictory assignments (surface and inside) in fact divide exactly according to the two subgroups of functional subfamily 7. Proteins 48-51 are "inside" at position 188 and "outside" at position 189, while proteins 52-54 are "outside" at position 188 and "inside" at position 189. This suggests that the helix is turned slightly in proteins 48-51 in comparison with proteins 52-54, and the pattern strengthens what would otherwise be a weak helical assignment. The segment also contains charge variation at position 191 (R or D) and at position 188 (V or K); this could be covariation indicating a helical structure, but analogous indicators in proteins with known structures are not highly reliable. Nevertheless, the assignment of a helix in this region is satisfactory for us to make it the preferred assignment in this region for this subfamily of proteins.

Two functional subfamilies, 3 and 5, have MPI=45%. In functional subfamily 3, the segment 185-190 could map as an amphiphilic helix, but the clarity of the map is compromised by uncertain assignments at positions 186 and 187. In functional subfamily 5, a helix is possible from positions 185-189, but the number of positions assigned is too small for the amphiphilicity to be significant.

Functional subfamily 1 has a higher MPI (50%), meaning that still more information is lost in the subalignment. Nevertheless, a pattern of amphiphilicity resembling a helix from position 85 to position 189, and possibly to position 193, is observed. The parse at position 190 is preferred based on a comparison with other subgroups, but is not absolute.

It is worth noting that surface assignments made for these subfamilies, are weaker than those made when the entire alignment is considered. For example, position 187 has an APC K (MPI=50%). With a subalignment of so few proteins and such little overall divergence in sequence, it is difficult to tell whether the conservation indicates a functional constraint on drift, or whether it merely indicates that insufficient sequence divergence has taken place for us to have (fortuitously) found a protein where the codon corresponding to this position had undergone mutation. For our purposes here, the position has been assigned as the weakest surface, simply based on the polarity. However, it is worth noting that K 187 is less reactive with acetic anhydride than other lysines in the protein, and therefore appears to be inside the protein fold.

To search for secondary patterns of conservation that might indicate that the active site segment exists in a particular standard secondary structure, the number of conserved subgroups in clusters of subgroups with different minimum pairwise were examined (Table 13). The data show a general trend; conservation falls off in both directions as one proceeds away from positions 182-184. However, in the amino direction, there is a pronounced increase in the conservation at position 179. In the carboxyl direction, the amino acids at position 187 are disproportionately conserved. Notably, these flank the CM1 F at position 183 in a helical projection, not the APC D. This is not unusual in active site helices, where the contact of the helix with the bulk of the protein is the most highly conserved, while the active site residues conserved in a portion of the helix "fade" in one direction as the active site helix moves away from the critical section. Remarkably, at both positions, the predominant amino acid is basic; at position 179 is a Lys, and at position 187 an Arg or Lys. Thus, there is weak evidence for a helical structure here.

The functional subfamilies can also be mapped out on a beta strand template to look for patterns of alternating properties. The underside of the beta strand is clearly more variable than the upper side. This is most evident in functional subfamily 6. but also in functional subfamilies 3, and particularly at positions 177-181. Further, the pattern of alternation from positions 177-181 is evident, making the beta structure more plausible for this segment. Thus, a weak case can also be made that this segment adopts a beta structure.

An expedient that has been used to resolve uncertain cases has been to reconstruct the sequences of ancient proteins using a rule of parsimony and examine their structures. By this process, weak evidence for helix can be found in this region; in the tyrosine kinases, positions 177, 181, and 188 are all indeterminate, and all lie on one side of a helical wheel. However, this region in the reconstructed protein is highly hydrophobic. Still stronger evidence for beta strand comes in subgroup 6, where 177, 179, and 185 are indeterminate, 181 nearly so; 183 is an APC F.

Segment 21. The deletion at positions 194-197 in functional subfamily 1 indicate that positions 193 and 198 are close in the remaining proteins. This region contains the autophosphorylation site for the tyrosine kinases (position 194), a site that may be near the active site. However, there is no evidence from the alignment that this segment is at the active site. Here again, this is most likely because of substantial functional adaptation that has led to sequence divergence within different functional subfamilies of proteins. Notably, the autophosphorylation site in the serine-threonine kinases (at position 199 in the next segment) does not align with the autophosphorylation site in the tyrosine kinases (position 194) in the alignment prepared by Hanks et al. (1988).

Segment 22. This segment appears to be another long broken beta strand. The alignment is not well anchored in this segment. Mobashery and Kaiser S. Mobashery, E. T. Kaiser, "Identification of amino acid residues involved in substrate recognition by the catalytic subunit of bovine cyclic AMP dependent protein kinase: Peptide-based affinity labels", Biochemistry 27, 3691-3696 (1988)! aligned the TWTLC segment against the NEYTA segment in the tyrosine kinases, based on the fact that the middle residue in both is the autophosphorylation site. The change in the alignment is extremely significant. If the residues from the tyrosine kinases presently matched with positions 198-204 remain, the conformation of the segment would be assigned as an active site coil. If not, it would be assigned as an active site beta strand from position 198-203.

The alignment from position 191-228 is heavily laden with parsing elements. Nine of the 29 positions involve deletion/insertions, and 18 of the 29 positions have P in at least one protein. Thus, a full 24 of the 29 positions (83%) are candidates of varying strengths for parses. There are several strong active site assignments. Position 209 is a CM6 P, canonically assigned as a secondary parse; a PP parsing sequence is found in proteins 46, 65, 80, and 84 (MPI<35%) confirms the assignment of a parse at this position. This parse is adjacent to an APC E, canonically designated an active site residue. A distributed parse is found at positions 203-204. Further, in positions 190-201, every residue is assigned to the surface, although the assignments at positions 200 and 201 are relatively weak. Likewise, the segment from 217-225 is largely assigned to the surface.

The pattern of variation and conservation suggests that this segment lies near the active site. From position 202-216, there are no surface assignments, and extremely little variability. Conserved strings and an APC E at position 210 support more strongly an assignment of this segment to the active site. There is ample experimental evidence to confirm this suggestion H. N. Bramson, N. Thomas, R. Matsueda, N. C. Nelson, S. S. Taylor, E. T. Kaiser, "Modification of the catalytic subunit of bovine heart cAMP dependent protein kinase with affinity labels related to peptide substrates", J. Biol. Chem. 257, 10575-10581 (1982); N. C. Nelson, S. S. Taylor, "Differential labeling and identification of the cysteine-containing tryptic peptides of the catalytic subunit of porcine heart cAMP-dependent protein kinase", J. Biol. Chem. 256, 3743-3750 (1981)!. As noted above, position 194 is the autophosphorylation site of several tyrosine kinases. Short peptides (e.g. LRRASLG) are phosphorylated by the catalytic subsegment of bovine cyclic AMP dependent protein kinase. When these are modified to introduce reactive groups, Thr 199 (alignment number) and Cys 201 are modified, and these residues are protected by substrate.

The principal difficulty in assigning conformations to segments such as these is to chose between an assignment as an active site coil, or as a set of short beta strands separated by bulges or turns. The first is favored in this case, as there are a large number of parsing elements in this segment. There is a conserved string in subgroup 2 (MPI=50%) (VTLWYR), a canonical indicator of an active site string. We have assigned an active site coil from position 201 to position 212, with the bent or broken at position 203-204 and again at position 209.

Segment 23: Positions 213-216 are deleted in most of the proteins in the alignment. Further, there is a deletion at position 224, a deletion that possibly can be moved by readjusting the alignment. The issue regarding the intervening segment (positions 217-223) is whether or not it adopts a standard secondary structure. Four consecutive surface assignments (positions 217-220) are canonically designated as a coil. Further, parsing elements are found at positions 218, 219, 220,, and 223. A coil is the canonical assignment, with position 221 serving as its hydrophobic anchor, a residue that points inside to hold the otherwise external loop in a defined conformation. There is a string SSS in protein 45 (positions 217-219) is also suggestive of a coil.

This conformational assignment may not hold for tyrosine kinases (functional subfamilies 8-12), which do not contain parsing strings or deletions in this region, and otherwise do not appear to be as non structured in this region as the serine-threonine kinases. However, given the problems with the alignment noted above, this matching may be deceptive.

Segment 24: The segment from 226-240 is entirely assigned to the inside; only at position 237 is there an extremely weak surface assignment (two variable subgroups MPI=50% with only one having a polar residue). The region has a large number of splits at low MPI's. Thus, this is an example of an example of an extended internal segment. Thus, the segment is either an internal beta strand or an internal helix. From the length of the segment, the internal helix assignment is canonical, although as indicated above, assigning internal segments is difficult for the method of the present invention.

The alternative assignment is a beta strand much too long to form a single secondary structural unit. For this assignment to be correct, the strand must be broken. The APC G at position 232 is a secondary parse, indicating one break. This creates a beta strand (7-8 residues, positions 225-232) followed by another beta strand (8 residues, positions 233-240), as both internal segments are now too short to be internal helices. Further, the first hypothetical beta strand might be broken by the APC D at position 227.

The patterns at the ends of the segment offer no confirmation for either assignment. Thus, as the beta strand has not met a burden of proof, the segment is canonically assigned a helical structure.

Segment 25. This segment is assign a coil conformation because of the distributed parse at positions 244-245, a presumed hydrophobic anchor at position 246, and the conserved G (secondary parse) at position 242. There is no evidence for active site residues in the coil. Positions 246 and 260 are close in space, as indicated by the deletion in protein 46. There is no evidence for active site residues in the coil.

Segment 26. This segment is not well anchored, and the single amino acid insertion at position 273 might well be collapsed by a shift of the alignment. This would move the beginning of the parse to position 276, possibly adding 2 more positions to the helix in the previous segment. This revised alignment is used in the analysis here.

There is textbook amphiphilicity from positions 260 to 272, suggesting an assignment of a 10 residue helix in this region (FIG. 3d). Helices of this type are only rarely misassigned, making this one of the strongest predictions in this structure. It then remains to determine how far this helix can be extended in either direction. The amphiphilic pattern is disrupted in the amino direction by the surface assignment at position 262 (which is on the inside face of the helix). Position 262 therefore may still be a part of the helix; as noted previously, amino acids on the inside ends of amphiphilic helices are often assigned to the surface. However, this implies that the helix does not extend more than a half turn further in the amino direction. This notion is confirmed by the scattered P's at positions 262, 261, and 260. Further, there is a parsing string PXP in positions 260-262 of protein 9, and the parsing string PP in positions 261-262 of protein 90. Based on this reasoning, the assigned helix begins at position 262.

The carboxyl end of the helix is more difficult to assign. Position 273 breaks the amphiphilic pattern of the helix, but the assignment is generated for the alignment with the deletion at position 273. This alignment is modified to remove the deletion in our final analysis. Likewise, the "correct" (presuming the helical wheel) assignment of position 274 to the surface is less strong in the realigned segment. To match parses (often in surface helices, the parses at the beginning and ends of the helix fall on the same side of a helical projection), there are several choices, as the potential parses at the beginning of the helix at positions 61, 62, and 63 each match with potential parses at the end (261 matches with 272; 262 matches with 273, and 263 matches with 274 in the rearranged alignment). Nevertheless, it is clear that the helix does not extend past position 275 (note the PPP parsing string in protein 42). Thus, positions 262-273 are assigned a helical structure, with a coil extending from 274 until the next parse.

Buechler and Taylor (1988) op cit.! found that the carboxyl side chains of D 260 is especially reactive with water-soluble carbodiimide. However, the side chain is not protected by substrate.

Segment 27: Positions 276-285 are deleted in protein 46, indicating that positions 275 and 286 are close in space in the folded structure in the remaining proteins. A large number of parsing strings within the region make the canonical assignment a coil for this segment. There is no evidence for active site residues in the coil.

Segments 28, 29, and 30. There is textbook amphiphilicity from positions 288 to 300, suggesting a 15 residue helix (a bit over four turns) in this region (FIG. 3e). Helices of this type and length are almost never misassigned, making this a "very strong" assignment. It remains to determine how far this helix can be extended in either direction. A deletion parse ending at position 285 suggests that the helix begins exactly at position 286. However, some of the amino acids can be moved from above the deletion to position 285 without significantly altering the significance of the alignment in this region. Indeed, in some proteins (e.g. proteins 52-54), the pattern of amphiphilicity appears to extend in this direction. Extending the helix back by one amino acid also superimposes the parses at each end of the helix. On these grounds, the amino end of the helix is extended to position 284 in the maximum helix (Table 11) in a readjusted alignment (some residues from positions 279 and 280 of proteins in functional subfamilies 1 and 2 are moved to position 284 in the alignment).

At the carboxyl end of the helix, amphiphilicity is broken at position 301. Further, a secondary parse at position 302 provides a plausible point to end the helix. Positions 299 to 304 are also a string of 6 consecutive surface assignments. Thus, positions 286-301 are assigned a helical structure in the preferred assignment for cAMP-dependent protein kinases (Table 11).

The conformations of the subsegments that immediately follow (positions 303-313) are difficult to assign. Positions 303, 304, 307, 309, 310, 313, and 314 are all assigned to the surface. Positions 306, 308, 311, and 312 are assigned to the inside. The most distinctive feature of this segment is the APC R at position 305. This is canonically assigned to the active site (although again, such assignments are only ca. 70% accurate).

Secondary parses at position 302 and 306 separated by 2 surface residues are canonically assigned as a loop. There are no active site strings that would confirm the APC R as an active site residue, even at relatively high MPI values. Thus, it might be argued that the APC R is an "anchor" for a loop not at the active site. However, in view of the wide range of functions performed by different members of this family of proteins, it is important to reexamine this point by functional class, assuming that the variation that is seen is adaptive in a substrate binding segment of the kinases. For example, in functional group 1 (50% MPI), one does not find the amount of variation that one would expect in a surface loop far from the active site. Thus, this is assigned as an active site coil.

The conformation of the following segment is especially problematic. Canonically, the presence of parsing strings (e.g., in functional subfamily 2, GSGPDGEP) and weak secondary parsing elements at positions 307, 309, 310, and 312 suggests that this segment is a coil with hydrophobic anchors at positions 308 and (in some subfamilies) 311 and 312.

Segment 31. Positions 314-318 are deleted in functional subgroups 3-6, implying that positions 313 and 319 are close together in space in the remaining proteins. There is no evidence for active site residues in the coil.

Segment 32. It is important to note that the sequences in this alignment are truncated; they continue past this point, often for some length. However, these carboxyl terminal extensions in the different proteins cannot be aligned. Canonically, it is simplest to assign this segment simply as an coil, as lies largely on the surface (all positions are assigned to the surface). However, the strength of the assignments varies as expected for an alpha helix. So does the hydrophobicity of the segment. Thus, it is possible that this segment is the beginning of a helix that will extend into the next section of the protein. It is important to test this by looking at the extended sequences of proteins, where they can be aligned.

In functional group 1, 2 and 8, the amphiphilic helix can be extended by one position (to 326). Group 8 has a secondary parse at what would be position 327 in the alignment. In the serine kinase groups, this position is occupied by a conserved W, a W that breaks the amphiphilic helix at this position. The sequence that follows also does not fit on the amphiphilic wheel; the next parsing segment in this class is at positions 337. While the method cannot discuss these structures without an alignment, there is no reason not to accept in this segment a short helix (7 amino acids, 319-325, with 319 being a weak surface). The P scattered in functional groups 1-5 either indicate that the helix is shorter in these groups (which makes the (in ADH, phospholipase, and other proteins, there are analogous single turn helices that show amphiphilicity) or, more probably in our opinion, a P in the first turn of an alpha helix.

Step 8: FIG. 4 contains a summary of the secondary structure predicted for protein kinase, together with those elements of the supersecondary and tertiary structure obtained in this example. Assembly of the secondary structural elements to form supersecondary structures requires that distant positions in the polypeptide chain be brought together in three-dimensional space. One way of obtaining information to assemble these elements comes from assignments of certain positions in the alignment to the active site, as these must be brought together in the folded protein. Unfortunately, the active site of protein kinase is quite large; therefore, these distance constraints are not very demanding. Nevertheless, active site assignments at positions 46, 113, 116, 156-162, 182, 199, 201, 210, and 305 have been used to assemble proposals for the folded structure shown in FIG. 4.

Unfortunately, protein kinases lack sulfide bonds that might be used to provide distance constraints connecting positions of the polypeptide chain that do not lie at the active site. Finally, no knowledge of the biochemistry of protein kinases has been used to constrain distances in this protein. Thus, the structural model shown in FIG. 4 is not complete.

Assembling the Secondary Structural Elements

To assemble the secondary structural units, positions 46, 67, 113, 116, 156-162, 182, 199, 201, 210, 237, and 305 are tentatively assigned as lying at or near the active site, suggesting that these points in the polypeptide chain come together in three dimensional space. Position 305 only weakly is assigned to the active site, however, and alternative models that place this residue at a position removed from the active site must also be considered.

The beta strands that are connected by short loops are then examined to see if evidence can be found that they lie antiparallel in a beta sheet. As discussed above, covariation analysis suggests that the two strands 84-93 and 103-111 lie antiparallel, with the side chain of the amino acid at position 87 of the first beta strand on the same side of the sheet as the side chain of the amino acid at position 108, and these two side chains in close proximity.

Further, based on active site assignments, beta strand 201-212 is arranged antiparallel to beta strand 226-240, although these long strands are almost certainly discontinuous, implying that this antiparallel arrangement need not extend along the entire strand.

Chemical crosslinking experiments suggest that the side chain of the amino acid at position 46 in the beta strand 43-48 is in close proximity to the side chain at position 182 in the beta strand 177-185; covariation analysis suggests that these these strands lie antiparallel (based on functional variation at positions 47 and 181, and the hydrophobic variable position 85 and the APC F at position 198). In contrast, no evidence can be found for any association of beta strand 12-22 with beta strand 43-48.

These considerations lead to the minimal structure shown in FIG. 4. It should be noted that in this Figure, the long beta strands are almost certainly bent. Further, constraints imposed by a need for a two dimensional representation distort the picture. Thus, as indicated on the Figure, the structure is further folded to bring together the active site residue at position 67 (near the ATP binding site) and att 113-116 (near the peptide binding site.

This picture can be modified by inferences drawn from chemical considerations of the reaction being catalyzed. First, the nucleophilic displacement is "in-line" with a trigonal bipyramidal phosphorus in the transition state lying between an attacking nucleophile and the departing beta-phosphate of ATP. The nucleophile (a serine, threonine, or tyrosine) must lose a proton to a basic residue at the active site. The pentacoordinate phosphate with additional negative charge must be stabilized by a positively charged residue on the protein. Finally, the Mg-ATP must be bound on the distal side of the phosphorus, with the divalent magnesium cation coordinated to the alpha and beta phosphates of ATP and to ligands on the enzyme. Finally, groups on the enzyme must form hydrogen bonds to the ribose ring hydroxyl groups, and present a hydrophobic pocket to hold the purine ring with a hydrogen bond to N(6).

What sorts of residues might one expect the enzyme to contribute for this sort of a catalytic effect? Some information bearing on this question can be obtained from the crystal structures of phosphoglycerate kinase R. D. Banks, C. C. F. Blake, P. R. Evans, R. Haser, D. W. Rice, G. W. Hardy, M. Merrett, A. W. Phillips, "Sequence, structure, and activity of phosphoglycerate kinase: A possible hinge-bending enzyme", Nature 279, 773-777 (1979)!, phosphofructokinase Y. Shirakihara, P. R. Evans, "Crystal structure of the complex of phosphofructokinase from Escherichia coli with its reaction products", J. Mol. Biol. 204, 973-994 (1988)! adenylate kinase D. Dreusicke, P. A. Karplus, G. E. Schulz, "Refined structure of porcine cytosolic adenylate kinase at 2.1 Å resolution", J. Mol. Biol. 199, 359-371 (1988)! , and pyruvate kinase H. Muirhead, D. A. Clayden, D. Barford, C. G. Lorimer, L. A. Fothergill-Gilmore, E. Schlitz, W. Schmitt, EMBO J. 5, 475-481 (1986)!.

Consider first the coordination of magnesium. In pyruvate kinase, the magnesium coordinates to the side chain of Glu 271 and two main chain carbonyl residues. Glu 271 is conserved in enzymes from cat, chick, rat, and yeast, is part of a conserved string of three, and lies in a turn at the end of a beta strand and two positions before the start of an alpha helix. In adenylate kinase, the side chain of Asp 93 (in the middle of a beta strand) appears to coordinate the magnesium. In phosphofructokinase, Asp 103 (in a conserved heptapeptide at the start of an alpha helix) coordinates magnesium. Asp 129 appears to bind water coordinated to magnesium in this protein. In phosphoglycerate kinase, Asp 374 (at the start of an alpha helix in a GGGD conserved string) coordinates the magnesium. Thus, we can expect that Asp and Glu residues (with perhaps a slight preference for the former) will also be involved in the coordination of magnesium in protein kinase, and that these residues can be identified by their pattern of conservation.

Interactions with the phosphate groups involves, not surprisingly, Arg and Lys residues, with perhaps a preference for the former. For example, in pyruvate kinase, Lys 114 and Arg 119 form salt bridges to the phosphate groups of the substrates. Arg 72 (in a beta strand) and Arg 293 (in a coil between a beta strand and an alpha helix) appear to be near the phosphorus electrophile. Both are completely conserved, the second in a string many amino acids long. In adenylate kinase, Lys 21 (in a turn or at the beginning of a helix) may be near the gamma-phosphate group. The residue is conserved in five sequences with pairwise identities from 24% to 52%. Arg 44, Arg 97, Arg 128, Arg 138, and Arg 149 are all conserved, and all point towards the active site cleft in this enzyme, not surprising considering the number of negatively charged groups that are brought together in the active site. In phosphofructokinase, Arg 72 seems to bind to the alpha-phosphorus of ATP as well as the phosphorus being transferred. In phosphoglycerate kinase, Lys 219 (at the start of an alpha helix) appears to bind the alpha-phosphate of ATP. The beta and gamma phosphates are found on the amino terminal end of a helix.

Coordination to ribose is varied. In phosphofructokinase, Tyr 41 (in a conserved dipeptide at the start of an alpha helix) provides the contact. In phosphoglycerate kinase, Glu 343 (in a coil at the end of a GVFE conserved sequence)) appears to make a hydrogen bond with the ribose hydroxyl groups. In alcohol dehydrogenases, the ribose is hydrogen bonded to an APC Asp 223 (at the end of a beta strand).

Similar roles may be assigned to various positions in protein kinase. In addition to the sequences themselves, information is available from chemical modification studies involving carbodiimide (for acidic residues), acetic anhydride (for Lys), and peptide analogs, summarized in Table 14.

There are only two candidates for a side chain to provide the positive charge to the gamma phosphate, K 46 and R 305. The chemical modification experiments with reactive analogs of ATP place the side chain K 46 near the gamma-phosphorus of ATP, and it seems not unreasonable to assign tentatively this role to this residue.

Identifying roles for the individual carboxyl groups is more difficult, as they can presumably serve to coordinate magnesium, or bind to the ribose, or act as a base abstracting a proton from the nucleophilic center of the protein substrate. Acidic residues under consideration here are of three types. First, there are two residues that are conserved, reactive with carbodiimide, and are protected by Mg-ATP (E 067 and D 182). Second, there are two residues that are conserved and do not react with carbodiimide (D 157 and E 210). Then there are two acidic residues that are not conserved, react with carbodiimide, but require both Mg-ATP and peptide to protect them (E 161 and E 237). Finally, there are two acidic residues that are not conserved, reactive, and are not protected by any sort of substrate combination (E 089 and D 260).

The last pair, E 089 and D 260, can be presumed not to lie at the active site, and therefore to be uninteresting for the present discussion. E 161 and E 237 might be presumed to lie between the substrate peptide binding site and the Mg-ATP binding site. Because they are not conserved, they presumably play no role in catalysis. However, these positions in the polypeptide chain should lie between positions 113-116 and 199-201 (the peptide binding site) and positions 157-160 and 67 (the Mg-ATP binding site).

In contrast, both D 157 and E 210 are absolutely conserved. Both do not react with carbodiimide, suggesting either that they are buried, or that they are for chemical reasons unreactive. The assignment of E 210 to the active site is not extremely strong. It is in a beta strand that appears to be inside the folded structure, but displays few of the sequence characteristics expected for a string at the active site. As noted above, absolutely conserved charged residues appear only ca. 70% of the time at the active site. It is possible that E 210 is one of the 30% of this type of position that does not.

In contrast, there is no question from the pattern of conservation in the region that D 157 lies at the active site. It must therefore be accessible to solvent under some conditions. We cannot say why it fails to react with carbodiimide. However, it is worth noting that the reaction with carbodiimide proceeds from the protonated form of a carboxyl group. A carboxyl group with a low pKa might not be expected to react. This is a behavior exactly opposite that expected for a carboxylate involved as a general base catalyst, suggesting that the residue at position 157 does not play this role. Presumably E 067 acts either as a ligand for magnesium or to form hydrogen bonds with the hydroxyl groups on the ribose ring.

The conservation of E 067 and D 182 indicates that these residues do play critical roles. However, in contrast to D 157, D 182 is extremely reactive with carbodiimide, suggesting that the side chain carboxylic acid has a high pKa. This implies, of course, that the conjugate base is relatively strong, as would be expected were this carboxylate to act as a base to deprotonate the nucleophile in the reaction. In this role, D 182 should be close enough to the position where the gamma-phosphate would bind in the enzyme-substrate complex that it forms a crosslink with K 46 when no substrate is present.

In this context, it is worth noting that the pKa values of the hydroxyl groups of serine and tyrosine are different by some six orders of magnitude. Thus, it is not expected a priori that the same basic group will abstract a proton from each. We had hoped to find in the protein kinase alignment a basic residue conserved in each functional subfamily but different between the two (a "functional split"), and use this pattern to identify the residue that acts as a base. Unfortunately, no such residue appears to exist in the alignment. Closest are positions 149 (mostly His in the serine/threonine kinases, and mostly Glu in the tyrosine kinases), but the conservation in each functional subfamily is not perfect. Thus, our preferred candidate for a residue that acts as a general base in the catalytic reaction is Asp 182.

These conclusions can be summarized in a model of the active site (FIG. 5) showing the transition state for the reaction and the presumed orientation of reactions involved in catalysis. While both E 67 and D 157 are shown coordinating magnesium, one of these two side chains might instead be forming hydrogen bonds to the hydroxyl groups of the ribose ring.

The models shown in FIGS. 4 and 5 are not, of course, complete representations of the conformation of the folded protein. However, provided that the two peptide binding sites are brought together in three dimensions, positions 67 and 157 brought to within bonding distance of a single magnesium, positions 161 and 237 placed between the peptide binding site and the ATP binding site, and positions 46 aligned with the gamma phosphate and 182 placed to abstract a proton from the reactive nucleophile, only a small number of folded forms are consistent with the constraints imposed by the assignments of secondary structure, the assignments of inside and surface positions (Table 10), and the covalent links of the polypeptide backbone. Further constraints are introduced if position 7 is placed near the active site.

Step 9: Using covariation analysis to help assemble supersecondary structure. In FIG. 4, the two beta strands (84-93 and 103-111) are oriented antiparallel to each other. This supersecondary structural assignment is based on covariation analysis, which suggested that positions 87 and 108 in protein kinase underwent variation that was not independent. The covariation is shown in Table 15. In particular, within functional subgroups 7 and 11, whenever position 87 suffers a substitution that lead to a loss of a charge, position 108 suffers the complementary substitution:

Remarks:

The key elements of the supersecondary and tertiary structure prediction are as follows:

(i) Protein kinase does not contain a domain that folds to give a Rossmann fold.

(ii) Rather, the cores structure of one domain contains consecutive β strands, aligned to form an antiparallel beta sheet.

(iii) Further, the distribution of helices does not follow the pattern suggested by the classical algorithms. These contrasts between predictions made by our method and by other methods is, of course, a point where the merits of the various approaches can be critically compared.

Example 2 Prediction of the Structure of Pathogenesis Related (PR) Proteins

Pathogenesis related (PR) proteins from plants come in several families. One family, often referred to as the PR1 family, has no known catalytic or physiological function. It is synthesized when the plant is subjected to environmental stress J. F. Bol, J. M. Linthorst, B. J. C. Cornelissen, "Plant pathogenesis-related proteins induced by viral infection", Ann. Rev. Phytopathology, 28, 113-138 (1990)!.

This example is provided to show the scope of the predictive method when only a very few sequences are available, and where the pairwise identity of the sequences is sub-optimal. There are far fewer sequences of homologous pathogenesis-related proteins than there are for protein kinases (Example 1). Further, the distribution of sequences on an evolutionary tree is far less satisfactory than with protein kinase. Finally, extremely little is known about the catalytic or physiological function of pathogenesis related proteins. The structure prediction in this example is developed in two steps, first with a small alignment of PR proteins alone (Table 16), and then with a larger alignment that includes the homologous TPX proteins from mammals and venom allergens from insects (Table 17). to show how the addition of sequences to an alignment can resolve ambiguities in assignments made from an alignment of only a few protein sequences.

Step 1: A set of sequences of homologous pathogenesis related proteins were found by searching the MIPS and SwissProt data bases. Errors in the sequences were corrected where necessary. An alignment was constructed using the DARWIN system from those sequences that were from plants.

Step 2: Two alignments are used in this example. Table 16 shows a small alignment of the sequences of the pathogenesis related proteins from plants. Table 17 shows a larger alignment of the sequences of the pathogenesis related proteins from plants together with their homologues, the TPX proteins from mammals and venom allergens from insects. Evolutionary trees showing the evolutionary relation between the proteins in the two alignments is given in FIG. 6. The evolutionary distance in the small alignment overall is only approximately 75 accepted point mutations per 100 amino acid residues, meaning that the strongest surface algorithms will not cover much of the surface. The evolutionary distance is larger in the big alignment, but the branching is still not as much as would be desired in the most preferable case. As is evident from the tree, the collection of sequences is rather small, and not optimally distributed in terms of evolutionary distance. Proteins a, d, and c form one subgroup with and MPI of greater than 90%, proteins g, e, and f form another subgroup with a similar MPI, and proteins b and h each stand alone in the tree.

Step 3. Divide the alignment into segments using parsing algorithms. Parses can be assigned based on gaps at positions 001-004, 053-054, 065-067, and 124. APC P's are present at positions 023, 131, and 140. Thus, the alignment is divided into 6 segments (excluding parsing segments): segment 1 (006-023), segment 2 023-052, segment 3 (055-064), segment 4 (068-123), segment 5 (125-131), and segment 6 (131-140).

Steps 4-6: When so few sequences are involved, the surface assignments made by the most reliable algorithms are few; surface assignments must therefore be made using less reliable algorithms to get adequate coverage of the alignment. Conservation of hydrophobic and functional amino acids is less significant with less overall sequence divergence; thus, the respective inside and active site assignments are less reliable. In this analysis of the PR proteins, it is understood that most assignments are weak. When strong assignments are made, they are capitalized.

In this example, it is recognized that the MPI values for subgroups that are examined concurrently are not uniform. In the first part of this example, there are only 3 subgroups that can be used. The first includes proteins a, b and c, the second proteins d, e, and f, and the third proteins g and h. This does not imply that a systematic understanding of the evolutionary relationship between the proteins in these subgroups can be ignored. Rather, this understanding is used to evaluate the reliability of the assignments made in Steps 4-6. Indeed, this example is intended to illustrate how to apply the method of the present invention when tidy subgroups with consistent MPI values cannot be obtained because of the small number of sequences in the alignment, and how, despite these constraints, useful assignments can be made with identified strengths. For example, surface assignment at position 013 is stronger than at position 007, as the former has 4 different polar residues, the latter only two.

Table 18 shows the residue by residue assignments for the PR proteins alone. In this Table, amphiphilic single variable positions are assigned to the surface if the variability occurs in the polar subgroup.

Step 7: Assign secondary structure.

An analysis of the secondary structure proceeds with each segment being examined individually. In this analysis, parsing segments are assigned as coils, and not discussed in detail.

Segment 1. Segment 1 starts at position 005 and ends at position 023. It contains both inside and surface assignments, and a helix wheel is shown in FIG. 7a. Amphiphilicity is found for positions 5-20, and a helix can be assigned tentatively to this segment.

Segment 2: Segment 2 starts at position 023 and ends at position 053. It is too long (31 residues) to form a single secondary structural unit. Indeed, a helix wheel covering the entire segment (FIG. 7b) shows consistent pattern of amphiphilicity over the entire length. Therefore, it might be subdivided using weaker parsing algorithms. A candidate for a secondary parse is found at position 039/040 (the NS dipeptide is a weak parse).

To find evidence for a helical structure in the first subsegment, a reduced helix wheel is plotted (FIG. 7b), where assignments are removed from the helical wheel from each end to search for a core segment that displays 3.6 position periodicity. The reduced wheel for segment 2 (FIG. 7b) shows perfect amphiphilicity between positions 028 and 046. The segment is then examined to determine how much longer the helical segment might be extended were assignments to be incorrect.

Position 027 (i_(N)) is a polar split assigned tentatively to the surface of the folded structure that lies within the inside arc of the helix projection. If this position is misassigned, then the next amphiphilic breaker occurs as position 025 (i_(N) -2), and no longer tentative helix is assigned in this direction.

In the other direction (FIG. 7c), position 047 (i_(C)) is a hydrophobic split assigned tentatively to the inside of the folded structure that lies within the surface arc of the helix projection. If this position is misassigned, then nothing contradicts amphiphilicity up to the APC S at position 050 (i_(N) +3). If position 050 is assumed to lie on the surface (remembering that absolute conservation is not extremely significant when the overall sequence divergence is only 050), and the amphiphilicity can be extended to the parse at position 053. However, most of the positions in this extension receive no assignments, and a helix wheel (FIG. 7c) constructed from position 53 backwards is not particularly convincing. Further, the conserved dipeptide HS is an indicator of an active site, and these are canonically assigned as coils. A coil assignment is also indicated by the parsing string SGGP in protein h (positions 50-53). The assignment is examined in light of more sequences in the section below.

Segment 3: Segment 3 starts at position 54 and ends at position 65. Surface and inside assignments are made for only 5 of the 12 positions that are assigned. These form an amphiphilic pattern on a helix wheel (FIG. 7d). However, the strongest characteristic of this segment is the large number (4, or 33%) of APC positions. This suggests an active site region, which is canonically assigned as a coil. Further, two positions (055 and 061) are APC G, parsing indicators that also indicate a coil. Finally, the GGG tripeptide seen in proteins a, d, and c indicates a coil. Thus, no assignment can be made from these sequences.

Segment 4: Segment 4 starts at position 067 and ends at position 124. This segment is clearly too long to form a single secondary structural unit in a protein of this size. Therefore, secondary parses are sought to subdivide the segment into subsegments before drawing helical wheels. Weaker parses are found at positions 070 (an isolated P), 081 (an isolated P), 092/093 (a GG dipeptide), and 111 (an APC G). The strongest of these parses is the APC G at position 111, and a tentative division is therefore made at this point. The segment preceding it is still rather long. For the purpose of drawing helix wheels, the distributed parse at position 092/093 is also used.

In the first half of this segment (designated segment 4ab), a helix wheel covering positions 67 through 92 displays no particular 3.6 position periodicity (FIG. 7e). A reduced helix wheel containing residues 69-79 does display amphiphilicity, however (FIG. 7e), and this section is tentatively assigned as an alpha helix. However, very few of the positions in this tentative helix have any strong surface assignments, and the designation is weak. Positions 82-86 display 2 residue periodicity, and are tentatively assigned as a beta strand.

In the second half of this segment (designated segment 4cd), a potential 3.6 position pattern of amphiphilicity might be seen in positions 103-113 (FIG. 7f). However, no reliable assignment can be made for this segment.

Segment 5: Segment 5 starts at position 124 and ends at position 131. No helical amphiphilicity can be seen in this segment (FIG. 7g). However, a 2 position alternating pattern of periodicity can be seen from position 125 to position 131, and this segment is assigned as a beta strand.

Segment 6: Segment 6 starts at position 131 and ends at position 140. No helical amphiphilicity can be seen in this segment (FIG. 7h). No 2 position alternating pattern of periodicity can be seen. However, given the large number of splits, this segment is tentatively assigned as a beta strand.

Adding More Sequences to the Alignment. So far, the secondary structure assignment has been built from a small alignment (Table 16) that contains only pathogenesis related proteins from plants. This increases the probability (but does not guarantee) that the proteins share similar functions. However, a search of the standard protein sequence data base discovers two other families of proteins that are clearly homologous to the PR proteins: sperm-coating glycoprotein (or testis-specific protein TPX-1) and a venom allergen from hornet. These can be added to the alignment (Table 17)

The secondary structure assignments can now be reviewed in light of a larger alignment that includes these additional sequences. This review is presented here for showing how the assignments made by the method of the present invention are influenced by the number of proteins in an alignment. Several questions can be addressed. With the additional sequences, does the parsing scheme hold up? Do the additional sequences reinforce the helical and other secondary structural assignments? Can stronger surface assignments be made? Do additional sequences contradict some active site assignments? Can the additional sequences be used to assign disulfide linkages?

In this discussion, only three subgroups will be considered. The first includes the PR1 proteins, the second the TPX proteins, and the third the venom allergens. The MPI values are understood to be those corresponding to those three groups. Special note should be made of how additional sequences allow more reliable algorithms to be used to assign surface and interior positions, and how assignments from less reliable algorithms become discounted in the presence of these stronger algorithms. For example, a polar split was used as an indicator of a surface position when using the small alignment. This was necessary, as the more reliable algorithms could not make enough assignments with few sequences. Below, polar split is no longer an automatic indicator of a surface position.

Step 3: Revising the Parses

Segment 1 has no counterpart in these additional sequences, and therefore cannot be reanalyzed. The significant alignment starts after the first parse (GVGP, 020-023).

A new parse is introduced into Segment 2 by a deletion of positions 041-044. The deletion is needed to align Cys 045 and His 049 in the new sequences, and appears to be reliable. This new parse confirms the weak parse made from the NS dipeptide in the small alignment truncates the helix assigned to segment 2 right before the end of amphiphilicity observed in the helix wheel.

The parse at position 053-054 is reinforced by insertion at this position.

A part of one protein contains a deletion at positions 61-63. This deletion could be removed by a rearrangement of the alignment, and this is done in the revised larger alignment.

The parse at positions 065-067 is reinforced by an insertion in the venom allergens at this position, and the parse is extended to position 069. The parse at position 069 can be moved two positions to the left by a minor rearrangement of the alignment. This leaves intact the assignment of a helix starting at position 068.

A new parse is introduced at positions 089-097 by scattered deletions. This reinforces the weak secondary parse suggested by the GG dipeptide at positions 092 and 093 in the small alignment. Further, it suggests that segment 4b should be assigned a standard secondary structure. A CM1 G at position 097 becomes a stronger parse, suggests that the secondary structural unit that follows begins at position 097.

A new parse is introduced by a single amino acid insertion between positions 117 and 118, right after a APC C, and a deletion at position 120. This further divides the long Segment 4, several residues after the end of amphiphilicity in the helix tentatively assigned for positions 97-111.

The parse at position 124 is shifted 2 positions to the left. This extends segment 5 to the left by 2 amino acids.

The parse at positions 131 and 132 is strongly reinforced. The APC P is found across the entire alignment.

Steps 4-7: Re-examine the surface, inside, active site, and secondary structure assignments in light of the additional sequence data

Segment 2a: Segment 2a was tentatively assigned as a helix. The helix assignment is confirmed by increasing strengths of both interior and surface assignments throughout the segment. The revised assignments are shown in Table 19a, and a helix wheel in FIG. 8a. The inside/surface split at position 033 is confirmed, but no covariation is found. The four amino acid deletion at positions 041-044 corresponds to one turn of the helix. Nevertheless, the helix definitely ends before the APC C at position 045. The APC D might be important as a helix breaking unit forming a hydrogen bonding interaction with the backbone atoms in proteins with this extra turn. Thus, the probability that this is an active site position is diminished. Thus, the helix is assigned from positions 023-041 (18 residues, 5 turns).

Segment 2b: Segment 2b was tentatively assigned as an active site coil. The additional sequences have changed the view of this segment. The revised assignments are shown in Table 19b, with the corresponding helix wheel in FIG. 8b. The APC D (position 44) and the APC S (position 50) are not conserved in the additional sequences; these positions therefore are no longer assigned to the active site. The parse truncates this segment before position 45. There is clearly no longer any helical amphiphilicity in this segment. the additional sequences in the alignment, the segment looks like a beta strand. Strengthening of surface assignments allows a clear alternating pattern indicative of a beta strand to appear. A distributed parse at positions 51-52 is evident. This is assigned as a beta strand from positions 45-50, followed by a coil.

Segment 3: Segment 3 was tentatively assigned as an active site coil. A beta strand is possible for positions 55-60. There is too much conservation for a coil in this segment. The revised assignments are shown in Table 19c. Possible extension of this strand to position 54 and to position 61. The distributed parse at positions 63 and 64 remain even if the alignment is rearranged to shift the gap to the right. If the gap is not shifted, the parse starts at position 62.

Segments 4a and 4b: Segments 4a and 4b were tentatively assigned as a helix and uncertain, respectively. With the additional sequences, amphiphilicity is observed on a helix wheel from positions 068-079. The revised assignments are shown in Table 19d, and a helix wheel in FIG. 7b. This corresponds to a helix 12 residues in length. Amphiphilicity is broken by a surface position at position 080, on the inside arc of the helix wheel.

Extension of the helix in the direction of the amino terminus is not possible, because of the deletion parse at position 065-067. In the other direction, amphiphilicity is not broken at position i_(C) +1 through i_(C) +4. Amphiphilicity is next broken at position 085, and then at position 087. Thus, a second tentative helix is assigned for positions 068-084 (17 residues). This segment is soon followed by a deletion parse (positions 089), so at best, the helix could extend only by one more turn (positions 068-088, 21 residues)

Deciding which assignment is correct involves inspection of the functional subgroups separately. It is conceivable for the length of a helix to be different in different families of proteins; indeed, this appears in this case with these proteins. Thus, we examine amphiphilic splits and positions at the border between the surface and inside arcs of the helix wheel.

The Amphiphilic split at position 077 lies in the border region. It is inside in the PR and TPX proteins, and surface in the venom allergens. Position 080 is surface in the PR and TPX proteins, and inside in the venom allergens. These two positions create the following situation. Amphiphilicity is retained in the PR and TPX proteins up until position 79. The helix in these proteins stops here. However, in the venom allergens, amphiphilicity continues until the parse. Thus, it is conceivable that in PR and TPX proteins, the helix is shorter than in venom allergens. The core structure (one one used to build a tertiary structural model) is always the shorter of the two. Nevertheless, in building the tertiary structural model, space must be left for a helix extension.

Segments 4c and 4d: Segments 4c and 4d were tentatively assigned helix and uncertain, respectively. The revised assignments are shown in Table 19e, and a helix wheel in FIG. 8e. With the additional sequences, the parsing has changed. Instead of starting this segment at position 93, one must start at position 97. A shift in the alignment to move the gap 2 amino acids to the left is clearly justified, Comments: The first part of this segment is clearly in the active site. It is a general rule that it is difficult to assign secondary structure in the vicinity of an active site, as the evolutionary constraints on sequence divergence imposed by functional demands of the active site tend to dominate those that reflect secondary structure. An interesting segment. There is a remarkable stretch of amphiphilicity from position 92 to position 120. If this is a helix, it is 29 residues long, or 8 turns. This compares to the helix in segment 2a of only 5 turns. The Amphiphilicity is broken at position 118. The issue could be resolved by disulfide linkages. The disulfide linkages suggest that positions 90 and 96 are joined. Thus, the helix cannot start before position 97. The 97-120 stretch is only 6.67 turns, and is more plausible in length.

Thus, we build a revised helical wheel. The question is whether the amphiphilic splits at position 108 and 114 covary. Judging by the amphiphilic splits, the PR proteins are more exposed at the top of the helix than the TPX proteins, and more buried at the bottom of the helix than the venom allergen, and that the TPX helix is more buried overall.

Next, we must worry about whether the helix should include the APC residues. Normally, this question is decided following a search for evidence that residues preceding the APC residues map onto a helix conformably with residues that follow. In this case, there is no evidence for this. Second, if we knew the catalytic function of the protein, we would build a model for the active site, and try to assign catalytic roles to the APC residues, to try to orient them in space relative to the presumed substrate. However, we have no idea what catalytic role any of these proteins perform. Thus, we must resort to the third test; do the APC residues themselves fall with 3.6 residue periodicity on a helical wheel? Of course, with 4 APC positions in a row (positions 98-101), this is not possible prima facie. Thus, the default is taken, that these residues do not form part of a helix this long, that they form an active site coil, and the helix begins at position 99. Now, where does the helix end? Treat parse at 118 as definitive, as it is reinforced by a Pro split at this position in the TPX proteins. The helix therefore extends from position 99-117, a total of 19 amino acids, or 5.3 turns.

Segment 5. The revised assignments for this segment are shown in Table 19f. The addition of a the additional sequences has prompted the alignment to be rearranged so that the deletion is moved to the left by two amino acids. The short inside segment (positions 123-126) is followed by alternating assignments up until position 131 (FIG. 8f). This leads to a prediction that this segment forms a beta stand in positions 123-131.

Segment 6. The revised assignments for this segment are shown in Table 19g. The segment has no obvious helical amphiphilicity. The consecutive surface positions (positions 137-139) and a parse at position 140 indicate a coil (or a last turn of a surface helix) following position 137. The large number of splits supports the canonical assignment of a beta strand for positions 131-136.

Step 8. As summary of the secondary structure prediction for pathogenesis related protein and its family is given in Table 20, and diagrammed in FIG. 9. PR proteins from plants have 6 cysteine residues that appear to be involved in the formation of 3 disulfide bonds. While the pattern of disulfide bond formation is not known, an analysis of the pattern of variation suggests that cysteine 90 is linked with cysteine 96. Further, the most plausible connectivity of the other cysteines is between 45 and 117, and 112 and 127. The simplest folded structure that is consistent with these assignments is a central beta strand flanked on both sides by helices.

                  TABLE 1     ______________________________________     A Comparison of the Secondary Structure Prediction and     Crystallographic Reality in the Catalytic Domain of Protein     Kinase.sup.§            Prediction        Reality                       Predicted  Actual Actual              Predicted                       Extent of  Secondary                                         Extent of              Secondary                       Secondary  Structural                                         Secondary     Alignment              Structural                       Structural Unit   Structural     Position*              Unit*    Unit*      in Crystal.sup.†                                         Unit.sup.†     ______________________________________     001-011  coil     41-51      beta 1 43-48     012-022  beta 2   52-62      beta 2 57-63     023-041  coil     63-67      coil   64-66     042-048  beta 3   68-74      beta 3 67-75     049-060  coil     75-84      alpha B                                         76-82     061-074  alpha C  85-98      alpha C                                         84-97     075-083  coil      99-101    coil    98-105     084-093  beta 4   102-111    beta 4 106-111     094-102  coil     112-115    coil   112-114     103-111  beta 5   116-123    beta 5 115-120     112-114  coil     124-126    coil   121-127     115-124  alpha D  127-136    alpha D                                         128-135     126-130  coil     137-139    coil   136-139     131-152  alpha E  140-161    alpha E                                         140-159     153-156  beta 6   162-165    beta 6 161-164     157-162  active site                       166-171    coil   165-170     163-166  beta 7   172-175    beta 7 171-175     167-176  coil     176-178    coil   176-177     177-186  beta 8   179-188    beta 8 + 9                                         178-191     187-200  coil     189-198    coil   192-198     201-212  beta     199-210    coil   199-210     213-225  coil     211-218    coil   211-217     226-241  beta     219-233    alpha F                                         218-233     242-261  coil     234-242    coil   234-243     262-274  alpha G  243-254    alpha G                                         244-252     275-285  coil     255-260    coil   253-262     286-301  alpha H  261-276    alpha H                                         263-272     302-306  coil     277-281    coil   273-281     307-312  beta     282-287    coil   282-287     313-318  coil     288-293    alpha I                                         288-293     319-325  coil     294-300    coil   294-300     ______________________________________      .sup.§ Bold entries indicate correct predictions.      Italicized entries indicate predictions that are possibly incorrect; see      text for discussion.      The underlined entry (alignment positions 226-241) is the single serious      error made in the secondary structure prediction.      Numbers in columns 3 and 5 refer to the positions in the sequence of the      catalytic subunit of recombinant mouse cAMPdependent protein kinase, the      protein whose crystal structure was solved, as given in Knighton et al.      (1991) op. cit.      Alignment position numbers (left column) do not correspond to these      numbers due to insertions and deletions.      *From Benner & Gerloff (1991) op. cit.      .sup.† From Knighton et al. (1991) op. cit.

§ Bold entries indicate correct predictions. Italicized entries indicate predictions that are possibly incorrect; see text for discussion. The underlined entry (alignment positions 226-241) is the single serious error made in the secondary structure prediction. Numbers in columns 3 and 5 refer to the the positions in the sequence of the catalytic subunit of recombinant mouse cAMP-dependent protein kinase, the protein whose crystal structure was solved, as given in Knighton et al. (1991) op. cit. Alignment position numbers (left column) do not correspond to these numbers due to insertions and deletions.

                  TABLE 2     ______________________________________     The One-letter Code for Amino Acids     ______________________________________     A           Ala        M         Met     C           Cys        N         Asn     D           Asp        P         Pro     E           Glu        Q         Gln     F           Phe        R         Arg     G           Gly        S         Ser     H           His        T         Thr     I           Ile        V         Val     K           Lys        W         Trp     L           Leu        Y         Tyr     ______________________________________

                  TABLE 3     ______________________________________     Alignment of Alcohol Dehygrogenases     ______________________________________             ----SSSSSSSG AASS             ---TTTTTTTTT TTNT             -AMIAAAAAAAK AATT             -------             005 GGGGGGGG GGGG SSASP             006 KKKKKKKK KKQQ IIIVD             007 VVVVVVVV VVII PPPPK             008 IIIIIIII IIII EEKEQ             009 KKKKKKKK KKKR TTTVL             010 CCCCCCCC CCCC QQQQA             011 KKKKKKKK KRRK KKKWA             012 AAAAAAAA AAAA GAGAV             013 AAAAAAAA AAAA VIVQF             014 VVVVVVVI VVVV IIIVH             015 LLLLLLLA ATAA FFFVT             016 WWWWWWWW WWWW YYYEH             017 EEEEEEEE EEEE EEEKG             018 ENLVLLPA AAAA SSNAG             019 KKKKKHHG GGGG HNKGP             020 KKKKKKKK KKKK GGGTE             021 PPPPPPPP PPPP KKKPN             022 FFFFFFFL LLLL LLLPV             023 SSSSSTTC SSVV EEHVK             024 IIIIIIII IIII YHYYF             025 EEEEEEEE EEEE KKKKE             026 EEEDEDDE EEEE DDDQE             027 VVVVVIIV VVVV IIIVV             028 EEEEEEEE EEEE PPPPP             029 VVVVVVVV VVVV VVVVV             030 AAAAAAAA AAAA PPPPA             031 PPPPPPPP PPPP KKKEE             032 PPPPPPPP PPPP PPPPP             033 KKKKKKKK QQQQ KKKGG             034 AAAAAAAA AAAK APPPQ             035 HHHYHHHH MMGH NNNDD             036 EEEEEEEE EEEE EEEEE             037 VVVVVVVV VVVV LLIIV             038 RRRRRRRR RRRR LLLLL             039 IIIIIIII VILI IIIVV             040 KKKKKKKQ KKKK NNNKV             041 MMMMMMMI IIII VVMIN             042 VVVVVVVI LLLL KKKRK             043 AAAAAAAA FYFF YYYYY             044 TTVVATTT TTTT SSSST             045 GGGGGGGS SASS GGGGG             046 IIIIIVVL LLLL VVVVV             047 CCCCCCCC CCCC CCCCC             048 RRGRRRRH HHHH HHHHH             049 SSTTSTSS TTTT TTTTT             050 DDDDDDDD DDDD DDDDD             051 DDDDEDDA VVVV LLLLL             052 HHHHHHHS YYYY HHHHH             053 VVVVVVAV FFFF AAAAA             054 VVVVVVVI WWWW WWWML             055 SSSSSSSD EEEE HHHKQ             056 GGGGGGGS AAAA GGGGG             057 TTTNNTSK KKKK DDDDD             --- WWWWW             ------F GGGG PPPPP             060 LLMLLLLE QQQQ LLLLL             061 VVVVVVFG TTTT PPPPP             062 TTTTTTTL PPPP VTVSA             063 PPPPPPPA VVLL KKKKK             064 LLLLLLLF FFFF LLLMM             065 PPPPPPPP PPPP PPPPP             066 VVVVVAAV RRRR LLLLL             067 IIIIIVVI IIII VVVII             068 AALLLLLV FLFF GGGGG             069 GGGGGGGG GGGG GGGGG             070 HHHHHHHH HHHH HHHHH             071 EEEEEEEE EEEE EEEEE             072 AAAAAGGA AAAA GGGGG             073 AAAAAAAA GGGG AAAAA             074 GGGGGGGG GGGG GGGGG             075 IIIIIIII IIII VVVVV             076 VVVVVVVV IVVV VVVVV             077 EEEEEEEE EEEE VVVVV             078 SSSSSSSS SSSS GGKAK             079 IIVVVVII VVVV MMLKV             080 GGGGGGGG GGGG GGGGG             081 EEEEEEEP EEEE EESEA             082 GGGGGGGG GGGG NNNLG             083 VVVVVVVV VVVV VVVVV             084 TTTTTTTT TTTT KKKKT             085 TTTTTCCN DDHD GGGDR             --E----             --D----             088 VVVVVVVV VVLL WWWFL             089 RRKKKKKK AAKQ KKKKK             090 PPPPPPPP PPPP IIVII             091 GGGGGGGG GGGG GGGGG             092 DDDDDDDD DDDD DDDDD             093 KKKKKKKK HHHH YYLRR             094 VVVVVVVV VVAV AAAAV             095 IIIIIIII LLLL GGGGG             096 PPPPPPPP PPPP IIIIV             097 LLLLLLLL VVVI KKKKK             098 FFAFFFFY FFFF WWWWW             099 TIITTSSA TTTT LLLLM             100 PPPPPPPP GGGG NNNNN             --- GGGGS             102 QQQQQQQL EEEE SSSSS             103 CCCCCCCC CCCC CCCCC             104 GGGGGGGR KKGG MMMLG             105 KKKKKEKK EEED AATSN             106 CCCCCCCC CCCC CCCCC             107 RSRRRRRK AAPR EEEEE             108 VVIVIIIF HHHH YYFMY             109 CCCCCCCC CCCC CCCCC             110 KKKKKKKL KKKQ EEEMM             111 HHNNNHHS SSSS LLSQK             112 PPPPPPPP AEEE GGGAA             113 EEEEEEEL EEEE NNHDE             114 GGSSSSST SSSS EEEEE             115 NNNNNNNN NNNN SSSPT             116 FLYYYFLL MMMM NNNLI             117 CCCCCCCC CCCC CCCCC             -----C-             119 LLLLLSQG DDDD PPPPP             120 KKKKKRTK LLLL HHDHH             121 NNNNNSKI LLLL AAAAI             122 DSDDDDNS RRRR SDDDQ             123 LLVLLLLN IIII LLLLL             124 SSSGGLTL NNNN SSSSS             125 MMNNNMQK TVTT GGGGG             ----PPPPPPPS DDDE             ----RRQRRRKP RRRR             ----GGGGGGGA GGGG             ------S             ------D             ------Q             ------Q VVVG             ----TTTTTTAL MMMM             ----MMLLLLLM IILI             ----QQQQQRLE AGNH             ----DDDDDDED DDDD             ----GGGGGGGK GGNG             ----TTTTTTTT KKKE             ----SSSRRSSS SSSS             ----RRRRRRRR RRRR             ----FFFFFFFF FFFF             ----TTTTTSST STSS             ----CCCCCCCC IIII             ----RRRRSKRK NSKN             ----GGRGGGGG GGGG             ----KKKKKKKK KQQK             ----PPPPPPQP PPPP             ----IIIIIIIV IIVI             ----HHHHHHHY YFHY             ----HHHHHHNH HHHH             151 FFFFFFFF FFFF YYYYY             152 LLLLVILF VVVL TTTTT             153 GGGGGSSG GGGG HHHVV             154 TTITVTTT TTTT DDDDD             155 SSSSSSSS SSSS GGGGG             156 TTTTTTTT TTTT SSSTT             157 FFFFFFFF FFFF FFFFF             158 SSSSSSSS SSSS QQQQQ             159 QQQQQQQQ EEEE QEQQH             160 YYYYYYYY YYYY YYFYY             161 TTTTTTTT TTTT AAATC             162 VVVVVVVV VVVV TTTII             163 VVVVVVVV MIVV AAAGA             164 DDDDDDDS HHHH DDDKN             165 EEEEEDDD VVAS AAAAA             166 IINNNIII GGGG VVIAT             167 SSAAAAAN CCCQ QQQLH             168 VVVVVVVL VLVV AAAAA             169 AAAAAAAA AAAA AAAST             170 KKKKKKKK KKKK HHKKI             171 IIIIIIII IIII IIIII             172 DDDDDDDD NNNN PPQPP             173 AAAAAGAD PPPP QQQDE             174 AAAAAAAD QEDD GGGNS             175 SSSSSSAA AAAA TTTVV             176 PPPPPPPN PPPP DDDPP             177 LLLLLLLL LLLL LLLLL             178 EEEEEDDE DDDD AAADE             179 KKKKKKKR KKKK QEEAV             180 VVVVVVVV VVVV VVVAA             181 CCCCCCCC CCCC AAAAA             182 LLLLLLLL VIII PPPPP             183 IIIIIILI LLLV IIIII             184 GGGGGGGG SSSS LLLLM             185 CCCCCCCC CCCC CCCCC             186 GGGGGGGG GGGG AAAAA             187 FFFFFFFF IIIL GGGGG             188 SSSSSSSS SSCS IIVII             189 TTTTTTTT TTTT TTTTT             190 GGGGGGGG GGGG VVVVC             191 YYYYYYYY LLLL YYYYY             192 GGGGGGGG GGGG KKKKR             193 SSSSSSSA AAAA AAAGA             194 AAAAAAAA STTT LLLLL             195 VVVVVVVI ILIL KKKKK             196 KKNNKKQN NNNN SSEEE             ----VVVVVVVN VVVV             198 AAAAAAAA AAAA AAASS             199 KKKKKKKK KKKK NNDGK             200 VVVVVVVV PPPP LLLAV             201 TTTTTTTT PAKK MRKRG             202 QQPPPPPP KKPK AAAPP             203 GGGGGGGG GGGG GGGGG             204 SSSSSSSS SSSQ HHDQE             205 TTTTTTTT TTSS WWWTW             206 CCCCCCCC VVVV VAVVI             207 AAAAAAAA AAAA AAAAC             208 VVVVVVVV VIII IIIII             209 FFFFFFFF FFFF SSSVP             210 GGGGGGGG GGGG GGGGG             211 LLLLLLLL LLLL AAAAA             --- AAAGG             213 GGGGGGGG GGGG GGGGG             214 GGGGGGGG AAAA GGGGG             215 VVVVVVVV VVVV LLLLL             216 GGGGGGGG GGGG GGGGG             217 LLLLLLLL LLLL SSSSH             218 SSSSSSSS AAAG LLLLL             219 VVAAVVVA AAAA AAAAA             220 IIIVVIVV AMAA VVVQV             221 MMMMMMII GEEE QQQQQ             222 GGGGGGGG GGGG YYYYY             223 CCCCCCCC AAAA AAAAA             224 KKKKKKKK RRRR KKTKK             225 AAAAAAAA ILII AAAAA             226 AAAAAAAA AASA MMMMM             227 GGGGGGGG GGGG GGGGA             228 AAAAAAAA AAAA YYYLM             ----AAAAAAAS SSSS             230 AAAAAAAA AAAA YYYLM             ----AAAAAAAS SSSS             232 RRRRRKRR RRRR RRRRR             233 IIIIIIII IIII VVVTV             234 IIIIIIII IIII LLLIV             235 GGAAAAAG GGGG GGGAA             236 VVVVVVVI VVVV IIIII             237 DDDDDDDD DDDD DDDDD             238 IIIIIIII LILF GGAST             239 NNNNNNNN NNVN GGGGG             240 KKKKKKKS PPSS EPEDD             241 DDDDDDDE SASK GGEED             242 KKKKKKKS RKRR KKKKK             243 FFFFFFFF FYFF EEEKA             244 AAAAAAAV EEED EEKAE             245 KKKKKKKK RKKK RTKEK             246 EEEEEEEA KKKE SSKQS             247 VVLLLLLL FFFF ILLLF             248 GGGGGGGG GGGG GGGGG             249 AAAAAAAA CCVV GGGAA             250 TTTTTTTT TTNT EEEEE             251 EEEEEDED EEEE VVVVV             252 CCCCCCCC FFFC FFFFF             253 VVIIIIIL VVVV IIIIL             254 NNNNNNNN NNNN DDDDD             255 PPPPPPPP PPPP FFFFF             256 QQQQQQQR KKKK TTTSK             257 DDDDDDDD DDED KKKKK             258 YYYYYYYL HHHH EETSE             259 KKKKKSTH NDDD KKKAA             260 KKKKKKKK KKKK DDNDD             261 PPPPPPPP PPPP IIMVM             262 IIIIIIII VVVI VVVVI             263 QQQQQQQQ QQQQ GSSAE             264 EEEEEEEE EEQQ AADDA             265 VVVVVVVV VVVV VVIVV             266 LLLLLLLI LLII LVQKK             267 TTKKKQQI AIIA KKEAA             ----EEEEEEEE EEEE             269 MMMMMMML MLMM AAAAC             270 SSTTTTTT TTTT TTTTT             271 NNDDDDDK NNND DNKPN             272 GGGGGGGG GGGG GGGGG             273 GGGGGGGG GGGG GGGGG             --L----             --G----             276 VVVVVVVV VVVV AAPAA             277 DDDDDDDD DDDD HHHHH             278 FFFFFFFF RRRR GGGAG             279 SSSSSSSA SSAS VIVVT             280 FFFFFFFL VVVV IIIIL             281 EEEEEEED EEEE NNNLV             282 VVVVVVVC CCCC VVVLL             283 IIIIIIIA TTTT SSSAS             284 GGGGGGGG GGGG VVVVT             285 RRRRQRRG NNSS SSSAS             286 LLLLLLLS IVIV EEEEP             287 DDDDDDDE NNQQ AAAKK             288 TTTTTTTT AAAA AAAPS             289 MMMMMMMM MMMM IIIFY             290 VVMMMTTK IIII EESQE             291 TTAAASSA QSSQ AALQQ             292 AASSSAAA AAAA SSSAA             293 LLLLLLLL FFFF TTTTA             294 SSLLLLLD EEEE RREEG             295 CCCCCSSC CCCC YYYYF             296 CCCCCCCT VVVV VCVVA             297 QQHHHHHT HHHH RRRRR             ----EEEEEASA DDDD             299 AAAAAAAA GGGG AAPSP             300 YYCCCCCW WWWW NNCHG             301 GGGGGGGG GGGG GGGGS             302 VVTTTVVS VVVV TTTST             303 SSSSSSSC AAAA TVVVM             304 VVVVVVVT VVVV VVVVV             305 IIIIIVIF VVVV VVVIV             306 VVVVVVVI VVVV VVVIV             307 GGGGGGGG GGGG GGGGS             308 VVVVVVVV VVVV MLLLM             309 PPPPPPPA PPPP PPPPP             310 PPPPPPPA HHSS AAAAA             311 DDDADNSG KKKK GGNNG             312 SSSSSAAS DDDD AAAAA             313 QQQQQQQK ADDD KKYFK             314 NNNNNNSG EQAA CCVLL             315 LLLLLLLL FFFF CSKKG             316 SSSSSSST KKKK SSSAA             317 MMMIIMVI TTTT DDEPD             318 NNNNNNNF HHHH VVVVI             319 PPPPPPPP PPPP FFFFF             320 MMMMMMME MMMM NNSTW             321 LLLLLLSE NNNN QHHTL             322 LLLLLLLL FFFF VVVVT             323 LLLLLLLI LLLL VVVVV             324 SSTTTLLI NSNN KKKRK             ----GGGGGGGG EEEE             ----RRRRRRRR RKRR             ----TTTTTTTT TTTT             ----WWWWWWWI LLLL             329 KKKKKKKN KKKK SSSMM             330 GGGGGGGG GGGG IIIIL             331 AAAAAAAT TTTT SSNNK             332 IIIVIIIF FFFF IIIII             333 FFFLYFFF FFYF KVVKC             334 GGGGGGGG GGGG GGGGG             335 GGGGGGGG NNNN SSSSS             336 FFFFFFFW YYYY YYYYH             337 KKKKKKKK KKKK VVVVV             338 SSSSSSSS PPPP GGGGG             339 KKKKKKKV RRRK NNNNN             340 DDEEEDDD TTTT RRRRR             341 SSCGSSAS DDDD AAAQI             342 VVVIVVVI LLLI DDDDD             343 PPPPPPPP PPPP TTTGS             344 KKKKKKKK NNNG RRRVI             345 LLLLLLLL VVVV EEEEE             346 VVVVVVVV VVVV AAAAA             347 AAAAAAAT EEEE LLLLL             348 DDDDDDDD LMKK DDDDE             349 FFFFFFFY YYYY FFFFY             350 MMMMMMMK MMMM FFFFV             351 AAAAAAAN KKKN AASAS             352 KKKKKKKK KKGK RRRRR             ----KKKKKKKK EEEE             ----FFFFFFFF LLLL             355 AASSSPPN EEEE GGGGG             356 LLLLLLLL VLLL LLLLL             357 DDDDDEDD EEEE VVIIV             358 PPAAAPPA KKKK KKKKK             359 LLLLLLLL FFFF SSSAP             360 IIIIIIIV IIII PPPPY             361 TTTTTTTT TTTT IIIFY             362 HHHHNHHH HHHH KKKKK             363 VVVVIVVT SSTT VVIKV             364 LLLLLLLL VVVV VVVAQ             365 PPPPPPPP PPPP GGGPP             366 FFFFFFFF FFFF LLLLF             367 EEEEEEED ASSS SSSQS             368 KKKKKKKK EEEE TSEDT             369 IIIIIIII IIII LLLLL             370 NNNNNNNS NNNN PPPPP             371 EEEEEEEE KTKK EEKQD             372 GGGGGAAA AAAA IIVIV             373 FFFFFFFF FFFF YYYFY             374 DDDDDDDD DDDD EEDER             375 LLLLLLLL LLYY KKLLL             376 LLLLLLLM MMMM MMMMM             377 RRHHRRRN ALLL EEEGH             378 SSSSSSAQ KKKK KKKQE             379 GGGGGGGG GGGG GGGGN             380 EKKKKKKK EEEE QQKKK             --- IIIII             382 SSSSSSSS GGSS VALAA             383 IIIIIIII ILII GGGGG             384 RRRRRRRR RRRR RRRRR             385 TTTTTTTT CCCC YYYYI             386 IIIVVVVI IIII VVVVV             387 LLLLLLLL IMII VVVLL             388 TTMTTTTI RRKT DDDED             389 FFFFFFFF MMMM TTTIL     ______________________________________

The alcohol dehydrogenases are divided into those from mammals (left column), plants (middle column), and fungi (right column). From left to right, the sequences (read vertically), are horse liver E, horse liver S, human class 1 alpha, human class 1 beta, human class 1 gamma, human class 2, moust, rat, maize 1, maize 2, pea, Arabadopsis, Saccharmoyces cerevisiae 1, Saccharomyces cerevisiae 2, Saccharomyces cerevisiae 3, Aspergillus, Schizosaccharomyces pombe.

                  TABLE 4     ______________________________________     Examples of Parsing Elements Drawn Elements Drawn from     an Alignment of Alcohol Dehydrogenase     ______________________________________     (i) Gap parse, confirmed in the undeleted     sequences     057 TTTNNTSK KKKK DDDDD     --- WWWWW     ------F GGGG PPPPP     060 LLMLLLLE QQQQ LLLLL     (ii) APC P parse     032 PPPPPPPP PPPP PPPPP     065 PPPPPPPP PPPP PPPPP     (iii) Two Examples of Distributed Parses     030 AAAAAAAA AAAA PPPPA     031 PPPPPPPP PPPP KKKEE     061 VVVVVVFG TTTT PPPPP     062 TTTTTTTL PPPP VTVSA     063 PPPPPPPA VVLL KKKKK     (iv) APC G parse     203 GGGGGGGG GGGG GGGGG     210 GGGGGGGG GGGG GGGGG     (v) Combination parse     176 PPPPPPPN PPPP DDDPP     365 PPPPPPPP PPPP GGGPP     (vi) Distributed combination parse     (vii) String parse     017 EEEEEEEE EEEE EEEKG     018 ENLVLLPA AAAA SSNAG     019 KKKKKHHG GGGG HNKGP     020 KKKKKKKK KKKK GGGTE     ______________________________________

                                      TABLE 5     __________________________________________________________________________     Examples of Surface Algorithms Applied to Two Parsed     Segments of an Alignment of Alcohol Dehydrogenase     Italicized assignments do not indicate surface position, but     are presented simply for reference.     __________________________________________________________________________     The alignment presented to reflect subgroups with MPI ≧ ca. 40%     365 PPPPPPPPPPPP GGGPP                         combination parse     366 FFFFFFFFFFFF LLLLF     367 EEEEEEEDASSS SSSQS                         Surface Algorithm 1, 2 variable subgroups     368 KKKKKKKKEEEE TSEDT                         Surface Algorithm 1, 2 variable subgroups     369 IIIIIIIIIIII LLLLL     370 NNNNNNNSNNNN PPPPP                         polar single variable     371 EEEEEEEEKTKK EEKQD                         Surface Algorithm 1, 2 variable subgroups     372 GGGGGAAAAAAA IIVIV     373 FFFFFFFFFFFF YYYFY     374 DDDDDDDDDDDD EEDER                         polar single variable     375 LLLLLLLLLLYY KKLLL                         Surface Algorithm 1, 2 variable subgroups     376 LLLLLLLMMMMM MMMMM     377 RRHHRRRNALLL EEEGH                         Surface Algorithm 1, 2 variable subgroups     378 SSSSSSAQKKKK KKKQE                         Surface Algorithm 1, 2 variable subgroups     379 GGGGGGGGGGGG GGGGN     380 EKKKKKKKEEEE QQKKK                         Surface Algorithm 1, 2 variable subgroups     ----------- IIIII   gap parse     382 SSSSSSSSGGSS VALAA     383 IIIIIIIIILII GGGGG     384 RRRRRRRRRRRR RRRRR                         APC R     385 TTTTTTTTCCCC YYYYI     386 IIIVVVVIIIII VVVVV     387 LLLLLLLLIMII VVVLL     388 TTMTTTTIRRKT DDDED                         Surface Algorithm 1, 2 variable subgroups     389 FFFFFFFFMMMM TTTIL     The alignment presented to reflect subgroups with MPI ≧ ca. 50%     365 PPPPPPPP PPPP GGGPP  combination parse     366 FFFFFFFF FFFF LLLLF     367 EEEEEEED ASSS SSSQS  Surface Algorithm 1, 3 variable subgroups     368 KKKKKKKK EEEE TSEDT  polar single variable     369 IIIIIIII IIII LLLLL     370 NNNNNNNS NNNN PPPPP  polar single variable     371 EEEEEEEE KTKK EEKQD  Surface Algorithm 1, 2 variable subgroups     372 GGGGGAAA AAAA IIVIV     373 FFFFFFFF FFFF YYYFY     374 DDDDDDDD DDDD EEDER  polar single variable     375 LLLLLLLL LLYY KKLLL  Surface Algorithm 1, 2 variable subgroups     376 LLLLLLLM MMMM MMMMM     377 RRHHRRRN ALLL EEEGH  Surface Algorithm 1, 3 variable subgroups     378 SSSSSSAQ KKKK KKKQE  Surface Algorithm 1, 2 variable subgroups     379 GGGGGGGG GGGG GGGGN     380 EKKKKKKK EEEE QQKKK  Surface Algorithm 1, 2 variable subgroups     --- IIIII                gap parse     382 SSSSSSSS GGSS VALAA     383 IIIIIIII ILII GGGGG     384 RRRRRRRR RRRR RRRRR  APC R     385 TTTTTTTT CCCC YYYYI     386 IIVVVVVI IIII VVVVV     387 LLLLLLLL IMII VVVLL     388 TTMTTTTI RRKT DDDED  Surface Algorithm 1, 3 variable subgroups     389 FFFFFFFF MMMM TTTIL     The alignment presented to reflect subgroups with MPI ≧ ca. 60%     365 PPPPPPPP PPPP GGGP P combination parse     366 FFFFFFFF FFFF LLLL F     367 EEEEEEED ASSS SSSQ S Surface Algorithm 1, 3 variable subgroups     368 KKKKKKKK EEEE TSED T polar single variable     369 IIIIIIII IIII LLLL L     370 NNNNNNNS NNNN PPPP P polar split     371 EEEEEEEE KTKK EEKQ D Surface Algorithm 1, 2 variable subgroups     372 GGGGGAAA AAAA IIVI V     373 FFFFFFFF FFFF YYYF Y     374 DDDDDDDD DDDD EEDE R polar single variable     375 LLLLLLLL LLYY KKLL L Surface Algorithm 1, 2 variable subgroups     376 LLLLLLLM MMMM MMMM M     377 RRHHRRRN ALLL EEEG H Surface Algorithm 1, 3 variable subgroups     378 SSSSSSAQ KKKK KKKQ E Surface Algorithm 1, 2 variable subgroups     379 GGGGGGGG GGGG GGGG N     380 EKKKKKKK EEEE QQKK K Surface Algorithm 1, 2 variable subgroups     --- IIII I               gap parse     382 SSSSSSSS GGSS VALA A     383 IIIIIIII ILII GGGG G     384 RRRRRRRR RRRR RRRR R APC R     385 TTTTTTTT CCCC YYYY I     386 IIIVVVVI IIII VVVV V     387 LLLLLLLL IMII VVVL L     388 TTMTTTTI RRKT DDDE D Surface Algorithm 1, 3 variable subgroups     389 FFFFFFFF MMMM TTTI L     The alignment presented to reflect subgroups with MPI ≧ ca. 70%     365 PPPPPPPP PPPP GGG P P                              combination parse     366 FFFFFFFF FFFF LLL L F     367 EEEEEEED ASSS SSS Q S                              Surface Algorithm 1, 2 variable subgroups     368 KKKKKKKK EEEE TSE D T                              polar single variable     369 IIIIIIII IIII LLL L L     370 NNNNNNNS NNNN PPP P P                              polar split     371 EEEEEEEE KTKK EEK Q D                              Surface Algorithm 1, 2 variable subgroups     372 GGGGGAAA AAAA IIV I V     373 FFFFFFFF FFFF YYY F Y     374 DDDDDDDD DDDD EED E R                              polar single variable     375 LLLLLLLL LLYY KKL L L                              Surface Algorithm 1, 2 variable subgroups     376 LLLLLLLM MMMM MMM M M     377 RRHHRRRN ALLL EEE G H                              Surface Algorithm 1, 2 variable subgroups     378 SSSSSSAQ KKKK KKK Q E     379 GGGGGGGG GGGG GGG G N     380 EKKKKKKK EEEE QQK K K                              Surface Algorithm 1, 2 variable subgroups     --- III I I              gap parse     382 SSSSSSSS GGSS VAL A A     383 IIIIIIII ILII GGG G G     384 RRRRRRRR RRRR RRR R R                              APC R     385 TTTTTTTT CCCC YYY Y I     386 IIIVVVVI IIII VVV V V     387 LLLLLLLL IMII VVV L L     388 TTMTTTTI RRKT DDD E D                              Surface Algorithm 1, 2 variable subgroups     389 FFFFFFFF MMMM TTT I L     The alignment presented to reflect subgroups with MPI ≧ ca. 80%     365 PPPPPPP P PPPP GGG P P                              combination parse     366 FFFFFFF F FFFF LLL L F     367 EEEEEEE D ASSS SSS Q S     368 KKKKKKK K EEEE TSE D T                              polar single variable     369 IIIIIII I IIII LLL L L     370 NNNNNNN S NNNN PPP P P                              polar split     371 EEEEEEE E KTKK EEK Q D                              Surface Algorithm 1, 2 variable subgroups     372 GGGGGAA A AAAA IIV I V     373 FFFFFFF F FFFF YYY F Y     374 DDDDDDD D DDDD EED E R                              polar single variable     375 LLLLLLL L LLYY KKL L L                              Surface Algorithm 1, 2 variable subgroups     376 LLLLLLL M MMMM MMM M M     377 RRHHRRR N ALLL EEE G H                              Surface Algorithm 1, 2 variable subgroups     378 SSSSSSA Q KKKK KKK Q E     379 GGGGGGG G GGGG GGG G N     380 EKKKKKK K EEEE QQK K K                              Surface Algorithm 1, 2 variable subgroups     --- III I I              gap parse     382 SSSSSSS S GGSS VAL A A     383 IIIIIII I ILII GGG G G     384 RRRRRRR R RRRR RRR R R                              APC R     385 TTTTTTT T CCCC YYY Y I     386 IIIVVVV I IIII VVV V V     387 LLLLLLL L IMII VVV L L     388 TTMTTTT I RRKT DDD E D                              Surface Algorithm 1, 2 variable subgroups     389 FFFFFFF F MMMM TTT I L     The alignment presented to reflect subgroups with MPI ≧ ca. 85%     365 PPPPP PP P PP PP GG G P P                              combination parse     366 FFFFF FF F FF FF LL L L F     367 EEEEE EE D AS SS SS S Q S     368 KKKKK KK K EE EE TS E D T     369 IIIII II I II II LL L L L     370 NNNNN NN S NN NN PP P P P                              polar split     371 EEEEE EE E KT KK EE K Q D                              polar single variable     372 GGGGG AA A AA AA II V I V     373 FFFFF FF F FF FF YY Y F Y     374 DDDDD DD D DD DD EE D E R                              polar split     375 LLLLL LL L LL YY KK L L L     376 LLLLL LL M MM MM MM M M M     377 RRHHR RR N AL LL EE E G H                              Surface Algorithm 1, 2 variable subgroups     378 SSSSS SA Q KK KK KK K Q E     379 GGGGG GG G GG GG GG G G N     380 EKKKK KK K EE EE QQ K K K                              polar single variable     - II I I I               gap parse     382 SSSSS SS S GG SS VA L A A     383 IIIII II I IL II GG G G G     384 RRRRR RR R RR RR RR R R R                              APC R     385 TTTTT TT T CC CC YY Y Y I     386 IIIVV VV I II II VV V V V     387 LLLLL LL L IM II VV V L L     388 TTMTT TT I RR KT DD D E D                              Surface Algorithm 1, 2 variable subgroups     389 FFFFF FF F MM MM TT T I L     The alignment presented to reflect subgroups with MPI ≧ ca. 90%     365 PP PPP PP P P P P P GG G P P                              combination parse     366 FF FFF FF F F F F F LL L L F     367 EE EEE EE D A S S S SS S Q S                              polar split     368 KK KKK KK K E E E E TS E D T     369 II III II I I I I I LL L L L     370 NN NNN NN S N N N N PP P P P                              polar split     371 EE EEE EE E K T K K EE K Q D                              polar split     372 GG GGG AA A A A A A II V I V     373 FF FFF FF F F F F F YY Y F Y     374 DD DDD DD D D D D D EE D E R                              polar split     375 LL LLL LL L L L Y Y KK L L L     376 LL LLL LL M M M M M MM M M M     377 RR HHR RR N A L L L EE E G H     378 SS SSS SA Q K K K K KK K Q E     379 GG GGG GG G G G G G GG G G N     380 EK KKK KK K E E E E QQ K K K                              polar single variable      II I I I                gap parse     382 SS SSS SS S G G S S VA L A A     383 II III II I I L I I GG G G G     384 RR RRR RR R R R R R RR R R R                              APC R     385 TT TTT TT T C C C C YY Y Y I     386 II IVV VV I I I I I VV V V V     387 LL LLL LL L I M I I VV V L L     388 TT MTT TT I R R K T DD D E D     389 FF FFF FF F M M M M TT T I L     __________________________________________________________________________

                  TABLE 6     ______________________________________     Predictions made by the "Evolutionarily Sophisticated" Surface     Algorithm for Alcohol Dehydrogenase            Residues      % of    %     MPI    identified    surface detected correct     ______________________________________     90%     8             4      100     85%    31            16      97     80%    45            24      96     70%    70            40      96     60%    91            46      95     50%    95            48      94     ______________________________________

                                      TABLE 7     __________________________________________________________________________     Examples of Interior Algorithms Applied to an Alignment of     Alcohol Dehydrogenase.     Iltalicized assignments do not indicate surface position, but are     presented simply for reference.     __________________________________________________________________________     The alignment presented to reflect subgroups with MPI ≧ ca. 40%     365 PPPPPPPPPPPP GGGPP                         combination parse     366 FFFFFFFFFFFF LLLLF                         Interior algorithm 4, hydrophobic single variable     367 EEEEEEEDASSS SSSQS     368 KKKKKKKKEEEE TSEDT     369 IIIIIIIIIIII LLLLL                         Interior algorithm 3, hydrophobic split     370 NNNNNNNSNNNN PPPPP     371 EEEEEEEEKTKK EEKQD     372 GGGGGAAAAAAA IIVIV     373 FFFFFFFFFFFF YYYFY                         Interior algorithm 4, hydrophobic single variable     374 DDDDDDDDDDDD EEDER     375 LLLLLLLLLLYY KKLLL     376 LLLLLLLMMMMM MMMMM                         Interior algorithm 4, hydrophobic single variable     377 RRHHRRRNALLL EEEGH     378 SSSSSSAQKKKK KKKQE     379 GGGGGGGGGGGG GGGGN     380 EKKKKKKKEEEE QQKKK     ----------- IIIII   gap parse     382 SSSSSSSSGGSS VALAA     383 IIIIIIIIILII GGGGG     384 RRRRRRRRRRRR RRRRR                         APC R     385 TTTTTTTTCCCC YYYYI     386 IIIVVVVIIIII VVVVV                         Interior algorithm 4, hydrophobic single variable     387 LLLLLLLLIMII VVVLL                         Interior algorithm 2, hydrophobic variable     388 TTMTTTTIRRKT DDDED     389 FFFFFFFFMMMM TTTIL     The alignment presented to reflect subgroups with MPI ≧ ca. 50%     365 PPPPPPPP PPPP GGGPP                         combination parse     366 FFFFFFFF FFFF LLLLF                         Interior algorithm 4, hydrophobic single variable     367 EEEEEEED ASSS SSSQS     368 KKKKKKKK EEEE TSEDT     369 IIIIIIII IIII LLLLL                         Interior algorithm 3, hydrophobic split     370 NNNNNNNS NNNN PPPPP     371 EEEEEEEE KTKK EEKQD     372 GGGGGAAA AAAA IIVIV     373 FFFFFFFF FFFF YYYFY                         Interior algorithm 4, hydrophobic single variable     374 DDDDDDDD DDDD EEDER     375 LLLLLLLL LLYY KKLLL     376 LLLLLLLM MMMM MMMMM                         Interior algorithm 4, hydrophobic single variable     377 RRHHRRRN ALLL EEEGH     378 SSSSSSAQ KKKK KKKQE     379 GGGGGGGG GGGG GGGGN     380 EKKKKKKK EEEE QQKKK     --- IIIII           gap parse     382 SSSSSSSS GGSS VALAA     383 IIIIIIII ILII GGGGG     384 RRRRRRRR RRRR RRRRR                         APC R     385 TTTTTTTT CCCC YYYYI     386 IIIVVVVI IIII VVVVV                         Interior algorithm 4, hydrophobic single variable     387 LLLLLLLL IMII VVVLL                         Interior algorithm 2, hydrophobic variable     388 TTMTTTTI RRKT DDDED     389 FFFFFFFF MMMM TTTIL     The alignment presented to reflect subgroups with MPI ≧ ca. 60%     365 PPPPPPPP PPPP GGGP P                         combination parse     366 FFFFFFFF FFFF LLLL F                         Interior algorithm 3, hydrophobic splitN     380     367 EEEEEEED ASSS SSSQ S     368 KKKKKKKK EEEE TSED T     369 IIIIIIII IIII LLLL L                         Interior algorithm 3, hydrophobic split     370 NNNNNNNS NNNN PPPP P     371 EEEEEEEE KTKK EEKQ D     372 GGGGGAAA AAAA IIVI V     373 FFFFFFFF FFFF YYYF Y                         Interior algorithm 4, hydrophobic single variable     374 DDDDDDDD DDDD EEDE R     375 LLLLLLLL LLYY KKLL L     376 LLLLLLLM MMMM MMMM M                         Interior algorithm 4, hydrophobic single variable     377 RRHHRRRN ALLL EEEG H     378 SSSSSSAQ KKKK KKKQ E     379 GGGGGGGG GGGG GGGG N     380 EKKKKKKK EEEE QQKK K     --- IIII I          gap parse     382 SSSSSSSS GGSS VALA A     383 IIIIIIII ILII GGGG G     384 RRRRRRRR RRRR RRRR R                         APC R     385 TTTTTTTT CCCC YYYY I                         Interior algorithm 3, hydrophobic split     386 IIIVVVVI IIII VVVV V                         Interior algorithm 4, hydrophobic single variable     387 LLLLLLLL IMII VVVL L                         Interior algorithm 2, hydrophobic variable     388 TTMTTTTI RRKT DDDE D     389 FFFFFFFF MMMM TTTI L     The alignment presented to reflect subgroups with MPI ≧ ca. 70%     365 PPPPPPPP PPPP GGG P P                         combination parse     366 FFFFFFFF FFFF LLL L F                         Interior algorithm 3, hydrophobic split     367 EEEEEEED ASSS SSS Q S     368 KKKKKKKK EEEE TSE D T     369 IIIIIIII IIII LLL L L                         Interior algorithm 3, hydrophobic split     370 NNNNNNNS NNNN PPP P P     371 EEEEEEEE KTKK EEK Q D     372 GGGGGAAA AAAA IIV I V     373 FFFFFFFF FFFF YYY F Y                         Interior algorithm 3, hydrophobic split     374 DDDDDDDD DDDD EED E R     375 LLLLLLLL LLYY KKL L L     376 LLLLLLLM MMMM MMM M M                         Interior algorithm 4, hydrophobic single variable     377 RRHHRRRN ALLL EEE G H     378 SSSSSSAQ KKKK KKK Q E     379 GGGGGGGG GGGG GGG G N     380 EKKKKKKK EEEE QQK K K     --- III I I         gap parse     382 SSSSSSSS GGSS VAL A A     383 IIIIIIII ILII GGG G G     384 RRRRRRRR RRRR RRR R R                         APC R     385 TTTTTTTT CCCC YYY Y I                         Interior algorithm 3, hydrophobic split     386 IIIVVVVI IIII VVV V V                         Interior algorithm 4, hydrophobic single variable     387 LLLLLLLL IMII VVV L L                         Interior algorithm 2, hydrophobic variable     388 TTMTTTTI RRKT DDD E D     389 FFFFFFFF MMMM TTT I L                         Interior algorithm 3, hydrophobic split     The alignment presented to reflect subgroups with MPI ≧ ca. 80%     365 PPPPPPP P PPPP GGG P P                         combination parse     366 FFFFFFF F FFFF LLL L F                         Interior algorithm 3, hydrophobic split     367 EEEEEEE D ASSS SSS Q S     368 KKKKKKK K EEEE TSE D T     369 IIIIIII I IIII LLL L L                         Interior algorithm 3, hydrophobic split     370 NNNNNNN S NNNN PPP P P     371 EEEEEEE E KTKK EEK Q D     372 GGGGGAA A AAAA IIV I V     373 FFFFFFF F FFFF YYY F Y                         Interior algorithm 3, hydrophobic split     374 DDDDDDD D DDDD EED E R     375 LLLLLLL L LLYY KKL L L     376 LLLLLLL M MMMM MMM M M                         Interior algorithm 3, hydrophobic split     377 RRHHRRR N ALLL EEE G H     378 SSSSSSA Q KKKK KKK Q E     379 GGGGGGG G GGGG GGG G N     380 EKKKKKK K EEEE QQK K K     --- III I I         gap parse     382 SSSSSSS S GGSS VAL A A     383 IIIIIII I ILII GGG G G     384 RRRRRRR R RRRR RRR R R                         APC R     385 TTTTTTT T CCCC YYY Y I                         Interior algorithm 3, hydrophobic split     386 IIIVVVV I IIII VVV V V                         Interior algorithm 4, hydrophobic single variable     387 LLLLLLL L IMII VVV L L                         Interior algorithm 2, hydrophobic variable     388 TTMTTTT I RRKT DDD E D     389 FFFFFFF F MMMM TTT I L                         Interior algorithm 3, hydrophobic split     The alignment presented to reflect subgroups with MPI ≧ ca. 85%     365 PPPPP PP P PP PP GG G P P                         combination parse     366 FFFFF FF F FF FF LL L L F                         Interior algorithm 3, hydrophobic split     367 EEEEE EE D AS SS SS S Q S     368 KKKKK KK K EE EE TS E D T     369 IIIII II I II II LL L L L                         Interior algorithm 3, hydrophobic split     370 NNNNN NN S NN NN PP P P P     371 EEEEE EE E KT KK EE K Q D     372 GGGGG AA A AA AA II V I V                         Interior algorithm 3, hydrophobic split     373 FFFFF FF F FF FF YY Y F Y                         Interior algorithm 3, hydrophobic split     374 DDDDD DD D DD DD EE D E R     375 LLLLL LL L LL YY KK L L L     376 LLLLL LL M MM MM MM M M M                         Interior algorithm 3, hydrophobic split     377 RRHHR RR N AL LL EE E G H     378 SSSSS SA Q KK KK KK K Q E     379 GGGGG GG G GG GG GG G G N     380 EKKKK KK K EE EE QQ K K K     - II I I I          gap parse     382 SSSSS SS S GG SS VA L A A     383 IIIII II I IL II GG G G G                         Interior algorithm 3, hydrophobic split     384 RRRRR RR R RR RR RR R R R                         APC R     385 TTTTT TT T CC CC YY Y Y I                         Interior algorithm 3, hydrophobic split     386 IIIVV VV I II II VV V V V                         Interior algorithm 4, hydrophobic single variable     387 LLLLL LL L IM II VV V L L                         Interior algorithm 2, hydrophobic variable     388 TTMTT TT I RR KT DD D E D     389 FFFFF FF F MM MM TT T I L                         Interior algorithm 3, hydrophobic split     The alignment presented to reflect subgroups with MPI ≧ ca. 90%     365 PP PPP PP P P P P P GG G P P                         combination parse     366 FF FFF FF F F F F F LL L L F                         Interior algorithm 3, hydrophobic split     367 EE EEE EE D A S S S SS S Q S     368 KK KKK KK K E E E E TS E D T     369 II III II I I I I I LL L L L                         Interior algorithm 3, hydrophobic split     370 NN NNN NN S N N N N PP P P P     371 EE EEE EE E K T K K EE K Q D     372 GG GGG AA A A A A A II V I V                         Interior algorithm 3, hydrophobic split     373 FF FFF FF F F F F F YY Y F Y                         Interior algorithm 3, hydrophobic split     374 DD DDD DD D D D D D EE D E R     375 LL LLL LL L L L Y Y KK L L L     376 LL LLL LL M M M M M MM M M M                         Interior algorithm 3, hydrophobic split     377 RR HHR RR N A L L L EE E G H     378 SS SSS SA Q K K K K KK K Q E     379 GG GGG GG G G G G G GG G G N     380 EK KKK KK K E E E E QQ K K K      II I I I           gap parse     382 SS SSS SS S G G S S VA L A A     383 II III II I I L I I GG G G G                         Interior algorithm 3, hydrophobic split     384 RR RRR RR R R R R R RR R R R                         APC R     385 TT TTT TT T C C C C YY Y Y I                         Interior algorithm 3, hydrophobic split     386 II IVV VV I I I I I VV V V V                         Interior algorithm 4, hydrophobic single variable     387 LL LLL LL L I M I I VV V L L                         Interior algorithm 3, hydrophobic split     388 TT MTT TT I R R K T DD D E D     389 FF FFF FF F M M M M TT T I L                         Interior algorithm 3, hydrophobic split     __________________________________________________________________________

                                      TABLE 8     __________________________________________________________________________     Alignment of the Sequences of Homologous Kinases     __________________________________________________________________________     protein #     >  5   10   15   20   25   30   35   40   45   50   55   60   65   70     75   80   85   90                |    |    |    |            |    |    |    |    |               |    |    |    |            |    |    |    |    |            3     001  41            DDGEKQHSS TTT-STST EDEEFDA GGGG AEESM DNQDTMGS SSSSEEG EESEEEEESI            TTSEEE TTA EEEEDSRL DNNK     002  42            QQDDNDDDD DDDDDDDD EENNSVK NVDD NNDRE DEDERGSQ EEEEQQQ SSSSSTTSEQ            DDEDDD EEE KKNKKQDI QNRN     003  43            FFFFFFFFF FFFFFFFF YYYYMYY YWWW YYYYY YYLLFFLY VVVVVVV LLILILLLIL            IIILLV FLL IIILLLIV LLLL     004  44            EDEEQQQQN NNNNSNSN QQEENND QREE KQTET QKVERTRQ MQQQCCC RRTQKKKKQM            TMIVVL KRR TTITNKVH VQSV     005  45            RRRRIIIII FFFFFFFF LLPPSII ILFF RKKPK LLQFNIFV LLLLLLL LLLLLLLLLL            MMMLLL KKK LMQLLLLF IFFL     006  46            IIKKLLMLI LLLLLLLI FYKKKEK VGVL LVIVE VILLVHVG SLLLLML EEEIVVVELM            KKHGGG IVG LSLRHLKN GGGG     007  47            KKKKRRRRD MMMMMMMK EEEEEEA KKEE EEEAK RDGDTGSP TKKKQHH VVRKKEEKRE            HHNEEE KKG RRALKRWE RKKK     008  48            TTTTTTTTT VVVVVVVV EDIIARL TTTT KKKEK KKKELAIL RRRRRRR KKRRRRRKKE            KKKQQR VVV EEPLLFEV TTTT     009  49            LLLLLLLLL LLLLLLLL LILLLLI LLVV VIIIV LIILLLIL IIIILLL LLLLLLLLLL            LLLIII LLL LLLLLLLI LLLL     010  50            GGGGGGGGG GGGGGGGG GGGGGGG GGGG GGGGG GGGGGGGG GGGGGGG GGGGGGGGGG            GGGGGG GGG GGGGGGGG GGGG     011  51            TTTTTTTTV KKKKKKKK KKRRGSR ETAA EEEVE REAHSPAS STTTASS QQTNAAAARS            GGGRRR SSM QQQSSSER SAAE     012  52            GGGGGGGGG GGGGGGGG GGGGGGG GGGG GGGGG GGGGGGGG GGGGGGG GGGGGGGGGG            GGGGGG GGG GGGGGGGG GGGG     013  53            SSSSSSSSG SSSSSSSS AAVVKKS SSSS TTTAT KTNNESAG SSSSGGG CCCQQQQQNQ            QQQNNN AAA SSSAAAAH AAAE     014  54            FFFFFFFFF FFFFFFFF FFSSFFF FTMM YYYYY YFSYFEYF FFFFFFF FFFFFFFFFF            YYYFFF FFF FFFFFFFF FFFF     015  55            GGGGGGGGG GGGGGGGG SSSSGGS GSGG GGGGA SSGGSGGG GGGGGGG GGGGGGGGGG            GGGGGG GGG GGGGGGGG GGGG     016  56            RRRRRRRRR KKKKKKKK VVVVAQR KCKK VVVTC ESTNECVS TTTTSSS EEDEEREEEV            EEDEEE TTR MMMEEEKC QKKK     017  57            VVVVVVVVV VVVVVVVV VVVVVVV VVVV VVVVV VVVVVVVV VVVVVVV VVVVVVVVVV            VVVVVV VVV VVVVVVVV VVVV     018  58            MMMMHHHHE MMMMMMML RRRRCFV KRKK YYYYY FYVSFFYY YFFFYYY WWWWWGWWFR            YYYFFF YYY YYYYYYFY VVVV     019  59            LLLLLLLLL LLLLLLLL RRRRTRR LLLL KKKKL EKKKQDKS KRRRKKK MMLMMWMMYR            EEESSS KKK EEEEEELH EEEK     020  60            VVVVIIVIV ASSSAAAA CCCCCLV AAAV AAGAG AAAVVSAG GGGGAAA GGGGGGGAGG            GAGGGG GGG GGGGGGAG AAAA     021  61            KKKKRRRRQ DEEEEDEE VVIITVE YKKK LRRRC IKLLESEI KRRLTTT TTTTYTYTKK            VVYRRR LIV NVITTQET TTTT     022  62            HHHHSSSSL RRRRRRRR KKHHEEH HHHH DHHDQ NDHHDHDR WWWWYYY WWWWYTYYWW            WWWLLL WWW AALAALCL AAAA     023  63            MKKKRRVNK KKKKRKRK VLKKKKR TARR LKKPH IIVKPPIV HHHHRHH NNNNNTNNRR            KKKRRR IIV RKKVLKHL HFYF     024  64            EEAAHHHHS GGGGGGGG LCPPSKA TKYQ RLTHS TTPPVDYA GGGGGGG GGGGNGGKNG            KRRAAA PPP DGSDDTND DDDH     025     ----- EDE IVFIIELN LLLL     026     ----- GGG IVPLLDLD SGIK     027     ----- EEE KKPGASPG HKHK     028     ----- KNN GDNVDEE- SESR     029     -- EEGGG-Q- QDDA     030     ---S--- -     031     ------- -     032     ------- -     033     ------- -     034     ------- -     035     ------- -     036     ------- -     037     ------- -     038     ------- -     039  65     --------- YYHDDD VVV APVGGEDK AAAGTTTT GSTST TGDTKYDD     040  66     ------ TTSNSTHHSS SGDNNN KKK EEDEEPKK TVAYGG TSSNTPGN     041  67     ---VVV TTTTTTTTII LNCTTT III TTRISQMI MLMTHR ENKVLQTL     042  68            HHYYYYYFT LLLLLLLL EEEEKVP KLVI IVIFK KYIIKRLP DDDDPPP RKKKKKKKDD            TTTLLP PPP RREKRRLH KKTT     043  69            YYYYYYYYF YYYYYYYY YYYYLWY VACC VVVVI CVVMYVYV VVVVVVV VVVVVVVVVT            VVIVVV VVV VVCVVVVC VVVV     044  70            AAAAAAAAA AAAAAAAA AAAAAAA AAAV AAAAA VAAAAIAA AAAAAAA AAAAAAAAAA            AAAAAA AAA AAAAAAAA AAAA     045  71            MMMMMMILM IVVVIIII AAVVAGI LIVI LMMLI VLKTVVVI VVVVIII IIVIVVVVVV            VVVVVV III VIIVVIVV VVVV     046  72            KKKKKKKKK KKKKKKKK KKKKKKK KKKK KKKKK KKKKKKKK KKKKKKK KKKKKKKKKK            KKKKKK KKK KKKKKKKK KKKK     047  73            IIIIVVVTI IIIIIIII IIIIVFM IIII KKKSE IKTEKAAH IVVVQQQ TTTTTSSTTM            TTASSS EVE TTTTTSAS MMMM     048  74            LLLLLLLLL LLLLLLLL IIIIIFI IIVV IIIVI LIIVLGLV LLLLVVV LLLLLLLMLM            LLLCCC LLL VVVLLLLL LLLL     049  75            DDDDKKKKK KKKKKKKK NNDDKKE NPNN RRRRK KYPRL-CE KKKKNNN KKKKKKKKRK            KKKRRR RRL NNNKKRKN KKKK     050  76            KKKKKKKKK KKKKKKKK TTVIKAT KIRR LLLVT PVVLV-KK VVVVKKK PPPPPQQPEE            EEEEEE EEK EEEKRKER SSPE     051  77            QQQQEEQHR DDDDDDDD KKTTQYK KRAA EEEPS VTEEK-DD VSSACCC GGGGGGGGGG            DDDTTT ANS SANGGGAI TTSN     052  78            KKKKIIQTH VVVVVVVV KKGGTSY V-TS SDSNE KSQLF-GR DQQQTTT TTTTTSSSTT            TTALLL TTT AAASAAST AAAA     053  79            VVVVVVVII VVVVIVII LLGGPAR L-KK EEEGF K-NDS-LI PPPPKKR MMMMMMMMMM            MMMPPP SSG SSTTTSED RHHS     054  80     --PPP PPA LMDDD-SI SALPVI SSGGKKE A-AA DSEGK K-NEG-NS TTTTNDT     055  81     --DDE KKE RRRQQ--G SDTSQQ AASS--- K-FY EEEGD K-SAP-ED PAAARLL     056  82     ------- - LLLMLT DDDDDDDD RRFF--- S-LL GGGGG I-T-K-KW EEEELRR     057  83     ------- - H KKKR DDDDDDDD     058     ------- -     059     ------- -     060  84            QQQQQQQQQ DDDDDDDD DDLLDEG M-RR VVVLL KSIKIWQN QQQQSSS SMSSSSSSSS            EAPLIL AAS EEEEEEAE EEEE     061  85            IIIIVVVVQ VVVVVVVV HHRRKKR QYDD PPPPD RPIFRYKG FAAARQQ PPPPVAPVTE            VLLKKK NNS RRRKKFRV KKRL     062  86            EEEEEEEEE EEEEDEDE QQEEEEE GAKK SSSIM EQNRNTKT QQQQRRR EEKEQGVEAD            EKHAAA KKE IITISAQS QEER     063  87            HHHHHHHHH CCCCCCCC KKAAMNV RSRR TTTSS IRQQRSLR AAAASSN AAASARPAAD            EDEKKK EEE EENEEEDQ AAAD     064  88            TTTTTTTTI TTTTTTTT LLTTVIC IITT AAATA KILILTQV FFFFFFF FFFFFLFFFF            FFFFFF IIF FFFFFLFF LLLL     065  89            LLLLNNNNR MMMMLMLM EELLMRE EGIV IIIVI IYVLLSAP RKKKWWW LLLLLPLLLI            LLLLLL LLL LLLLLLQL MMML     066  90            NNNNDDDDS VVVVVVVI RRKKLDS RMRR RRRRR LNRMQHRM NNNNAAA QQEEEAAAQE            KEAQQQ DDR NNSKKQRT SSSS     067  91            EEEEEEEEE EEEEEEEE EEEEEEE EEEE EEEEE EEEEEEEE EEEEEEE EEEEEEEEEE            EEEEEE EEE EEEEEEEE EEEE     068  92            KKKKRRRRK KKKKKKKK AAVVIIL IIAA IIIVV NLLLVALV VMMMLLL AAAAAAAAAA            AAAAAA AAA AAAAAAAA AAAA     069  93            RRRRLLRRQ RRRRRRRR RRDDESR SLSS SSSAK LNSESRAV AQQQNNN QQQQNNNNAK            AAARKR YYY SSSHHQEI KKKN     070  94            IIIIMMMMI VVVVVVVV IIIIVIV YMLL LLLLY RLIVILLL VVVVVII VIVILLLVIV            VIIIII VVI VVVLLLLI IIVV     071  95            LLLLLLLLM LLLLLLLL CCLLMML LMGG LLLLL GLVLQLHL LLLLAAA MMMMMMMMMM            MMMLLL MMM MMMMMMLM MMLL     072  96            QQQQSSKSQ AAAAAAAA RRRQNNR RRQQ KKKRQ GYKHRRAK RRRRRRR KKKKKKKKKT            KKKKKK AAA KKKSSSTK SSSK     073  97            AAAAIILIG LLLLLLLL LLKKQCR LLII EEERE TINKARRK KKKKLLL KKLKTQQTKK            EEDQQQ SGS GEEKKNMD HHYQ     074  98            VVVVVVVVA LPPPGMGG LLVVLLV LLLL LVLLM NMVCLLVV TTTT--- LLLLLLLLFL            IMLYYY VVE FFFFFFLF LLLV     075     ------- GGG-     076     ------- -     077     ------- -     078     ------- -     079     ------- -     080     ------- -     081  99            NNNETTESH KKK-RKRK KKGGNHR RRYY KNREQ IGPNGDSD RRRRRRH RRRKQQQQRQ            KKHSSN DGE TNDNDKQS PQNN     082 100            FFFFHHHHS PPP-PPPP HHHHHHH HHHH DRHHH ISHSHHHF HHHHHHH HHHHHHHHHH            HHHHHH NSH CCTHHHHH HHHH     083 101            PPPPPPPPD PPP-HPHP PSPHRPA PPPP DSPPP TSEPDPPS VVVVDDD EDDDDQPDNP            PPKPPP PPV HHYPPEQP LEMP     084 102            FFFFFFFFF FFFFFFFF NNNNNKN HNHH NNNNN LRNYHAYG NNNNNNN KKKKKRRKRN            NNNNNN HYN HHHNHNHN HHHH     085 103            LLLLIILII LLLLLLLL IIIILLI IIII ICIVV LVIIIIIV IIIIIII LLLLLLLLLL            LLLIII VVL VVVIIIIV VIIV     086 104            VVVVIIIIV TTTTTTTV VVIIIVI ILCC VVVVI AAIVVLII LLLLVVI VVVVVVVVVV            VVVVVV CSL VVVLLVVL VVVI     087 105            KKRRRRRRR QQQQQQQQ RRQQQQQ KRRR RRSRE VPTDEPTR LLPLRRR QPQQRRRKAQ            QQRRRR RRK RRRKKRRS NNNN     088 106            LLLLMMMML LLLLLLLL LLLLLCL LLLL LLLLL VLFFLLLL FFFFVVV LLLLLLLLLL            LLLLLL LLL LLLQLLFL LLLL     089 107            EEEEWWWWY HHHHHHHH HHKKYVV YYFF YLQMI KCYYNLHL MMMMVVV YYYYYYYHYY            LILIII LLL LLLLLVFL LLLY     090 108            FFYYGGGGR SSSSSSSS DDDDADE DDEE DDDDD DDGGDDRD GGGGAAA AAAAAAAAAG            GGGGGG GGA GGGGGGGG GGGG     091 109            SSASTTTTT CCCCTCTC SSTTAAV VVMM IIVVI PAAASLVW YFFFAAA VVVVVVVVVV            VVVVVV IIV VVVVVIVI AAAA     092 110            FFFFFFFFF FFFFFFFF IIYYIFF IWCC VLLCF VKYFWHLF MMMMSSS VVVVVVVVCC            CCCCCC CCC VVCCCCCC CCCC     093 111            KKKKQQQQK QQQQQQQQ SSEEEEE KTTT HHMAM SRYFEVEE TTTTTTT SSSSTTTTST            TTTTTT LLM SSSLLFTL TTTS     094 112     VNIHVTR KRRRRRR EEEERQQKQK RRHQQQ TTS KQRLLDER KHIQ     095 113     -- GGGNNTGS GGGD VMMMPVPM EENNPKQ KHSS DEDSY     096     ------E -     097     ------- -     098     ------- -     099     ------- -     100     ------- -     101 114            SSSSQQRQK DDDDDDDD GGTTHAE DQNN HSSID RDNGGGDD DPPPSSS EEEEEEEEER            PPAQQQ SSS QQQEEERG GGGG     102 115            NNNNQQNQY RRRRRRRR HFFFENR EHHH KKRKN TQEAFVAS NGGGLLL PPPPPPPPPP            PPPPPP TTQ PPPPPPPS PPPP     103 116            LLLLIIIVL LLLLLLLL HHFFIIV IMFF LLLVL PVIVLTIF LFVFGGG IIIIIIIIII            FFFIII VVM TTAQQILP IVTL     104 117            YYYYFFFFY YYYYYYYF YYFFVVY IYYY YYYTN AIIYYCYV AAAATTT YYYYYYYYYY            YYYYYY QQM LLLYYSLL YLLL     105 118            MMMMMMMMM FFFEFFEF LLLLLMM MLMM LLLLL LAIMMLVL IIIIIII IIIIIIIIII            IIIIII LLL VVVILLMV IVVL     106 119            VVVVIIVVL VVVVVVVV IVVVFVV VALL VVIVV IVLCQVVI VIIIIII VVVVIIIIVV            IIIVVV IVI VIVIIIVV IIII     107 120            MMMMIMMMM MMMMMMMM FFFFMLM ILFF FFFFL FLMMVLLL TTTTMMM TTTTTTTTQT            TTTMMM TTT MMMLLMFL TTTV     108 121            EEEE-DDDE EEEEEEEE DDDDEEE EEEE EEEEE EPEEEPQE QQQQEEE EEEEEEEEEE            EEEEEE QQQ EEEEEEEP EEEE     109 122            YYYY-YYYA YYYYYYYY LLLLYML YYYY FFFHF HYYYLKYR WWWWFFF YFFYYYYFYY            FFFLLL LLL LLLLLHYY YYYY     110 123            VVVVLIIIC VVVVVVVV VVMMIVA AVVV LLLVL VYSMCYCP CCCCGGG MMMMMMMMMM            MMMVVV MMM MMMMMMMM CCCA     111 124            PAPPKEEEL NNNNTNTN TTKKEST GPSS DDSDP NPDDEQPE EEEEGGG SSCNAEEASK            TSCQQQ PPP ATKEEERK RCCK     112 125            GGGGVGGGG GGGGGGGG GGKRGGG NDGG LMMQT NHCGNANP GGGGNNN KKHKKNNKKH            YHNGGG FYL HRKGGAHH YYYY     113 126     ---- TEGGG-GV SSSSVVV GGGGGGGGGG GGGGGG GGG GGVGGGGG GGGG     114     ------- -     115 127            EEEEREEEE DDDDDDDD EEEEEEE EEQQ DDDDD DESSSDDD SSSSTTT SSSSSSSSSS            NNNDDD CCC DDFDDDDD DDDS     116 128            MMMMLLLLL LLLLLLLL LLLLLLL LLLL LLLLL FFLLLLLL LLLLLLL LLLLLLLLLL            LLLFFF LLL LLLLLLLL LLLL     117 129            FFFFFFFFW MMMMMMMI FFFFFFF FFLL KKKRE KRDDDYFF YYYYHHH LLLLLVVLLL            LLLLLL LLL KKKLLLNR VLLR     118 130            SSSSSSSST YYYYYYYF EEDDEED DHHH RKKTV QTKKRTTD KHHHQQQ DDDDDDDDDN            DDETTS DDD SSSTSSRN DNNG     119 131            HHHHLLLLI HHHHHHHQ DDYYRRR YYYY YYYYV LFIIFYYF HHHHVVV FFFFFFFFFF            YFYFFF YHY YYYYYYFF YFFF     120 132            LLLLLLLLL IIIIIIII IILLIII IIII MMLLI YYLYLLII LLLLIII LLLLLLLLLL            LLLLLL VVV LLLLLLLI LLLL     121 133            RRRRRRRRR QQQQQQQQ VVTTVII VRII EDDDK QRSDESTT HHHHYYY KKKKKKKKRR            RRRRRR RRR RRRRRRRR HRRR     122 134            RRRRKKKKD QQQQQQQQ AAEEDDA QKQQ GRSKD TDVEEREE VVVVGDG GENDSTTSER            ESRTTS EEN SSAKGASN RRRE     123 135     -YSQRKR QAAAAAA EGPGDPPDGH CATEEE HNN LLHAAAHE NKKS     124 136     -KSGLKG EDDDATT TDEEESSEDE NGDGGG KRR RRRRRRGT KARR     125 137     -REQNVA TTTTGRR GGGGGGGGGK RRKAAP DGD PPPMKAPH HEDK     126     -FILPY- KRRRHSS KKQRGIISRT QESRRH NRK EERAQTDN TASV     127     -- AMSTKSA- FMFML ETL---     128     -- EEGFFTK- LLIP     129 138            FFFFFFFFF FFFFFFFF YYLLLLF MLIL LLMLF LLFILLGL FFFFLLL LLLLVLLQLG            VLLLLL ILI PPPLLLLP LLLL     130 139            SSSSPPPPE KKKKKKKK SSSTTTT SSRK GDDPT TPNDDGNQ QDDDSSS RKRKLTNPHN            NDPRRK GGS TSTTTSGT SEDT     131 140            EEEENNNND EEEEEEEE EEEEEEE EEEE APSAP DIEEERSE MMMMLLL LLLLLIVLFM            AAPVMM SSS LLYLLLLV YLLM     132 141            PPPPPPPPS PPPPPPPP AAKKVRR QRHH DRSEA YKLPFPHD FVVVGGG PPPPPNNPEG            VVIKKK QQK QSGVTSGK TREG     133 142            HHHHVVVVT QHHHHQHV DDEEDED EEQH ILLTD EGTQRQLL QQQQKKK QQQNKKKKDL            VAITTE YDA EKRDDEQD DDDD     134 143            AAAAAAAAT AAAAAAAA AATTTCA AAAA VVVII IIILVIIA LLLLCCC LLLLLLLLLL            LLLLLL LLL MMILLLLL LLLL     135 144            RRRRQKKKR VVVVAVAV SSRRMIT RARR KQKKK RKSAWAKR IIIILLL VVVVILLIIL            LLVLLI LLL IIYVLLLI VLLI     136 145            FFFFIFFFF FFFFFFFF HHKKVKR RHKK KKSDA YKKFKATG DDDDKKK DDDDDDDDYD            YYQQQK NNN QQQDDAAG GHSS     137 146            YYYYFYYYY YYYYYYYY CCIIFYV FYFF FFYLW YYIIIVVF IVVVYYY MMMMFMMFIM            MMMMMM WWW MMVLIMVF FFFF     138 147            AAAAAAAAT AAAAAAAA IIMMVML FLAA MTLMM LIAALSFF AAAASSS AAAASAASAC            AAAVVM CCS AAACCCAG SSSA     139 148            AAAAAAAAA AAAAAAAA QQRRRRQ QSRR MYYRL FWYNVRLW RRRRLLL AAAAAAAATI            TTSGGE VMT AGIVLISL YSYW     140 149            QQQQEEEEC EEEEEEEE QQAAQQM QQGG QQQQM EEGAEQQQ QQQQDDD QQQQQQQQQQ            QQQDDN QQQ EEEDDDQQ QQQQ     141 150            IIIIVVVVV IIIIIIII IILLIIV IIII LLIFT LLVVVLLV TTTTVVI IIVVIIIIVV            IIIAAA III IIIIIVVV VVVI     142 151            VVVVCCICV SAAAASAA LLLLCSL ILAA CVLLL LLLIALIL AAAAVVV AAAAAAAAAS            SAAAAA AAA AAASCAAA AAAS     143 152            LLLLLLLLE IIIIIIIA EEEEDED SDSS KNQRR KRNHLSSE QQQQNNN SDEAEEEESK            SSSAAA KKK DDDKKNAK NQKQ     144 153            TTTTAAAAA GGGGGGGG AAVVGGG AAAA GGGGG AAGGGAAA GGGGGGG GGGGGGGGGG            AGGGGG GGG GGGGGGGA GGGG     145 154            FFFFLLLLF LLLLLLLL VVIIIVV VVLL IVILV LLLLLVVV MMMMLLL MMMMMMMMMM            MMMMMM MMM MMMCCCMM MMMM     146 155            EEEEEEEEA FFFFFFFF LLCCLER EAIQ ANVDY DKDKQDER DDDDLLL AAAAAAAAET            ESSEEE NSS AAAVVSVK DAAQ     147 156            YYYYYYYYY FFFFFFFF HHATFYY YHYY YFFFH YFHEFYHH YYYYFFF YYYYYFFFYY            YYYYYY YYY YYYYYYYY FFFY     148 157            LLLLLLLLL LLLLLLLL CCLLMIL CCLL CCCLC CVLLIICC LLLLLLL VIMIIIIILL            LLLLLL LLL LLLLLLLL LLLL     149 158            HHHHHHHHH HQQQHHHH HHHHHHH HHHH HHHHH HHYKHHHH HHHHHHH EEEEEEEEEE            EEEEEE EEE NNAEEEAA AAAA     150 159            SSSSSSASS KSSSNKNT QQKKKKA RRAA SSSAR SSREHRSN AAAASSS RRRRREEQSR            KSASSS DDE AAARKDGS SSSE     151 160            LLLLKKHKK RKKKQRQK MMLLMQL HFNN HRRNN MKQQKQVC KKKKQQQ MMMMKRQRKH            KRRKKK RVK KNKMMMLK KKKM     152 161            DDDDDDNDG GGGGGGGG GGNNRGG KRNN RRRCF GGKNNGGG NNNNSSS NNNNNNNNQN            NNHCCH RRR KKKHRHHK NNNK     153 162            LLLLIIIII IIIIIIII VVIIVII IFII IIVII IIIIYIIV IIIIIII YYYYYYYYLY            FYFCCC LLL FFFFFFFF CCCL     154 163            IIIIIIITI IIIIIIIL VVVVLVT VRVV LILVL MIIIVIYL IIIIVLL VIIIIIIIII            IIIIII VVV VVVIIVVV VIIV     155 164            YYYYYYYYY YYYYYYYY HHHHHHH HHHH HHHHH HHHHHHHH HHHHHHH HHHHHHHHHH            HHHHHH HHH HHHHHHHH HHHH     156 165            RRRRRRRRR RRRRRRRR RRRRLLR RRRR RRRRR RRRRLRRR RRRRLLL RRRRRRRRRR            RRRRRR RRR RRRRRRRR RRRR     157 166            DDDDDDDDD DDDDDDDD DDDDDDD DDDD DDDDD DDDDDDDD DDDDDDD DDDDDDDDDD            DDDDDD DDD DDDDDDDD DDDD     158 167            LLLLLLLLL LLLLLLLL LLLLLLL LLLL LLLLL VIIVLILI MLLLLLL LLLLLLLLLL            LLLLLL LLL LLLLLLLL LLLL     159 168            KKKKKKKKK KKKKKKKK KKKKKKK KKKK KKKKK KKKKKKKK KKKKKKK RRRRRRRRAA            AAAAAA AAA AAAAAAAA AAAA     160 169            PPPPPPPPP LLLLLLLL PPPPPPP PLII PPPPP PPPPPTPD SSSSPPP AAASAAAAAA            AAAAAA AAA AAAAACTA AAAA     161 170            EEEEEEEEE DDDDDDDD EEEEEEE EEEE QQQEN HTSTAEEE NNNNAAA AAAAAAAARR            RRRRRR RRR RRRRRRRR RRRR     162 171            NNNNNNNNN NNNNNNNN NNNNNNN NNNN NNNNN NNNNNNNN NNNNNNN NNNNNNNNNN            NNNNNN NNN NNNNNNNN NNNN     163 172            LLLLIIIIL VVVVVVVV LLIIIIL LIII LLLIL VFVIVIII IIIIIII IIIIVIIIVC            CCCCCC VVV CCCCCCCC VVII     164 173            LLLLLLLLI MMMMMMML LLLLLML LLMM LLLLL MLLLMFML FFFFLLL LLLLLLLLLL            LLLLLL LLL MMMLLLLM LLLL     165 174            IIIILLLLL LLLLLLLL LLLLCCY LIII IIIVF IFICIIVI LLLLIII VVVVVVVVIV            VVVVVV VVV VVVVVVVL ILLV     166 175            DDDDDDDDD DDDDDDDD AADDVVY DKSS NDDTS DNNSTNGD HHHHSSS GGGGSSSSGG            GGSTTT KKR AAASSTGD CTTA     167 176            QQHHKKRKH SSSSASAA SSDDNNH EVDS KKDSP HLSAFTNL EEEEEEE EEENEDDAES            EDEEEE TSL HEDVEEQE ENHE     168 177            QQQQNNNNR EEEEEEED KKDNTKP HNSS DEKGD EEKNEPDS GGGGQQK NNRGSTTSNE            NNHKKK PPL DDDKKSGK GGGG     169 178            GGGGGGGGG GGGGGGGG LLMMTTG LESG GGGGG NLGQGEGR LLLLDDD LLLLLLLLNN            HKNNNN QN- FFLDQTLF KHRR     170     ---YG-- - -NG T     171     ---GS-- - --HTD     172     ---ST-- - ----S     173     ---CD-- -     174     ---SR-- -     175     ---RR-- -     176     ----R-- -     177 179            YYYYHHHHY HHHHHHHH AANNLSK NQEE NNTTQ KRQTTDTE TTTTVVV VVAIMSSVVV            LLIVVT HHA TTTIVTVT LVIK     178 180            IIIIIIIIA IIIIIIIV VVIIVII VIII LLIVI LGIVLIVI VVVVCCC CCCCCCCCAV            VVVLLL VVG VVVVVVVV VATM     179 181            QQQQKKKKK KKKKKKKK KKKKKKI KKKK KKKKK RVKKKVYK KKKKKKK KKKKKKKKKK            KKKKKK KKE KKKKKKKK KKKK     180 182            VVVVIIIIL IIIIIIII LLLLLLI IIII LLLLV LLLLILLL IIIIIII VIIIIIIIIV            VVIIII IID IIIIIIIV IIII     181 183            TTTTTTTTV AAAATATA AATTIIT AAII GAAAA IVCCGGAI GGGGSSS AAAAAAAACA            AAASSS TTH GGGGGGGA CGCS     182 184            DDDDDDDDD DDDDDDDD DDDDDDD DDDD DDDDD DDDDDDDD DDDDDDD DDDDDDDDDD            DDDDDD DDD DDDDDDDD DDDD     183 185            FFFFFFFFF FFFFFFFF FFFFFFF FFFF FFFFF WFFFFFFF FFFFFFF FFFFFFFFFF            FFFFFF FFF FFFFFFFF FFFF     184 186            GGGGGGGGG GGGGGGGG GGGGGGG GGGG GGGGG GGGGGGGG GGGGGGG GGGGGGGGGG            GGGGGG GGG GGGGGGGG GGGG     185 187            FFFFFFFFF MMMMMMMM LLFFLLL LMLL LLLLL LLVVMALS LLLLCCC LLLLLLLLLL            LLLMMM LLL MMMLLLML LLLL     186 188            AAAAAAAAA CCCCCCCC AASSAAA SASS AAAAA AASSAAAG AAAASSS AAAAAAAAAA            SAASSS AAA TTTAAASA AAAS     187 189            KKKKKKKKK KKKKKKKK IICCRRS NTNN RRRRR EEKGSCTA TTTTEQQ RRRRRRRRRR            RRRRRR KRK RRRRRRRR RRRR     188 190            RRRRYYEYK EEEEEEEE EEQQRRA IVII ASAIA FAKNVFTL VVVVKKK LLLLVLLVVY            LLFEEQ LLL DDDDDDDD DDDD     189 191            VVVVVVVVI HNNNNHNN VVLLYLR MEYF FFFYI YQLLWVEL KKKKLLL IIIIIIIIIV            MMMEEE LLL IIIIIIIM IIIV     190 192            KKKKPPQPG MIIIVMVI EQDQNEK TPDD GGGSP HMIVPQPK STTTEQQ EEKEEEEEAL            TRKAAE GDS YYYYYYYY MMKY     191 193            GGGGDDTDF MWWWFMFV GGPPPSK DNSY VVIYA PDNAVGYD RRRRDDD DDDDDDDDDD            GDEDDD AIS EEEKKKSD RNNE     192 194            RRRRVVVVG DDDDPDPG EDGGNAG GDRR PPPQP GYSSPSST WWWWLLL NNDNNNNNDD            DDDGGG EDD TTTNNSTK DDDE     193 195            TTTTTTTTK GGGGGGGD QQEEEGD NSKK LLIMH QKILRRSV SSSSLRR EEEEEEEEEQ            TTTVIV EES DDDDDDDE SSSD     194     L VVVVSVSK QQKKKSD FCQQ RRRAE ESAAGS-Y GGGGCGC YYYYYYYYYY YYYYYY KTN     YYYYYYYY NNNS     195     T TTTTTTTT AALLLLC LLLL ANVLI YM---S-- SAAAFRR TTNTTTTTCT TTTAAA EEE     YYYYYYYY YYYY     196     I---P-- QQQQQQP AAPAAAAAPS AAAAAS YYY RRRRRRRS IIVV     197     S---F-- QPPPTA- RRCRRRRRKS HHHSST HHK KKKKKKVV SVVK     198 196            WWWWYYWYW RKKKRRRK FFRRKKK KEHH TTTTT NADKMYDT EEEEYPH QQQQEEEEQG            AAAGGG EDA GGTGGGGT GGGS     199 197            TTTTTTTTT TTTTTTTT GGEEVVT TVTT HHHPS VNTTEGFD QQQQPPH GGGGGGGGGG            GGGLLM GGG KKKEEERG SNNQ     200 198            LLLLLLLLF FTFFFFFF FFVVNLT SYFF EEEVN RRFNRIGF PPPPLII AASAAAAAST            AAARRK GGG GGGGGGTA TAAG     201 199            CCCCCCCCC CCCCCCCC AACCFFC CCCC IIVVV VAVIEACD TSSSGGG KKKKKKKKRK            KKKQQQ KKK LLLLLLMK YRRR     202 200            GGGGGGGGG GGGGGGGG GGGGGGG GGGG VVVVV AGGGGGGG GGGGGGG FFFFFFFFFF            FFFVVI VVM LLLLLLLL LLLI     203 201            TTTTTTTTT TTTTTTTT TTTTTTT SSSS TTTTT STTCDTST SSPSTTT PPPPPPPPPP            PPPPPP PPP PPPPPPPP PPPP     204 202            PPPPPPPPP PPPPPPPP PPPPPPP PLLL LLLLR RRSQCILR VVVVYYY IIIIIIIIVI            IIIVVV III VVVVVVIV LVVV     205 203            EEEEDDDDE DDDDDDDD GGSSEEE NHYY WWWWW YGTSEDFV LLLLTTT KKKKKKKKKK            KKKKKK KKK RRRRRRRK KKKK     206 204            YYYYYYYYY YYYYYYYY YYYYFFY YYFF YYYYY FFYYYTYY WWWWHHH WWWWWWWWWW            WWWWWW WWW WWWWWWWW WWWW     207 205            LLLLIIIIV IIIIIIII LLLLLVI ALAA RRRRR KRMMINMS MMMMRQQ TTTTTTTTTA            TTTTTT MML MMMMMMMM MMMM     208 206            AAAAAAAAA AAAAAAAA SSAASAA AAAA AASAA GASAAASP AAAAAAA AAAAAAAAAP            AAAAAA AAA ASPAASPA AAAA     209 207            PPPPPPPPP PPPPPPPP PPPPPPP PPPP PPPPP PPPPPPPP PAAAPPP PPPPPPPPPP            PPPPPP LLL PPPPPPPL PPPI     210 208            EEEEEEEEE EEEEEEEE EEEEEEE EEEE EEEEE EEEEEEEE EEEEEEE EEEEEEEEEE            EEEEEE EEE EEEEEEEE EEEE     211 209            IIIIVVVVI IIIIIIII VVIIVVV VILL VVVVL LVRRVVCW VVVVLIL AAAAAAAAAV            SGGAAA SSC SSSSSSSS SSSS     212 210            IIIIVVIVI IIIIIIII LLIIVIL IVLL LLLLL LLILLLQI IITILLL AAAAIIIIIL            LLLLLL III LLLLLLIL IIIL     213     ------- - VM-K--RR RRRR---     214     ------- - MM---     215     ------- - QQ---     216     ------- -     217 211            LLLLSSTSL AAAAAAAL RRNGNNV SSKK GGGQG DKQDAASY DDDDKKK LLLLNNNNIN            AAANNN LLR KKRMIVLQ FFFF     218 212            SSSSTTTTN YYYYYYYY KKHHYYR GHAA GSSSA YCGRNGSH NPPPGGG YYFYFYYFYY            YYFYYY HRN DDDDDDYT NDND     219 213            KKKKKKKKK QQQQQQQQ DEPPDEK KKNQ KRATK QGNAHDSR NNNNEEE GGGGGGGGGT            NNNGGG RRR GGGGGGRQ SCCH     220 214            GGGGPPPPG PPPPPPPP PAGGQPP LPPP QHR-H MAVTLPFY PPPPGII RRRRCTTSKR            KKTRRR IRV VVVIVLKK LVVI     221 215            YYYYYYYYH YYYYYYYY YYYYIIY YYYY YYYYY YQYYYYAH FYYYVAA FFFFFFFFFF            FFFYYY YFF FFYFFFFF YYYY     222 216            NNNNNNNND GGGDGGDG GGGGSGT ARTT SSSAT DSSTDTTG SSSSTTT TTTTTTTTSS            SSSSSS TTT TTSTTTTT TTTT     223 217            KKKKKKKKI KKKKKKKK KKKKDYN GGGG TTTTS YTIVKTAR FFFFPPP IIIIIIIIIS            ITSSSS HHS TTSTNTTT TVFT     224     ------- - P     225 218            AAAASSSSS SSSSSSSS PPEEKES EPEE GGPPA SKKQPTPS QQQQKKK KKKKKKKKKK            KKKEEE QQK SYAQHQEK LQEQ     226 219            VVVVIIVVA VVVVVVVV VVVVTTV VAVV VVVVI LIGSAVNA SSSSAAA SSSSSSSSSS            SSSSSS SSS SSSSSSSS SSSS     227 220            DDDDDDDDD DDDDDDDD DDDDDDD DDDD DDDDD DDDDDDDA DDDDDDD DDDDDDDDDD            DDDDDD DDD DDDDDDDD DDDD     228 221            WWWWWWWWY WWWWWWWW LIMMMMM VVVI IIIMI MIVIIIVV VVVVIII VVVVVVVVVV            VVVVVV VVV MVVVVVVV VVVV     229 222            WWWWWWWWW WWWWWWWW WWWWWWW WWWW WWWWW WWWWWWWW YYYYYYY WWWWWWWWWW            WWWWWW WWW WWFWMWWW WWWW     230 223            AAAASSSSS AAASSAAA AASSSSA SSSS SSSSS SSSSSSAS SAAASSS SSSSSSSSSA            AAASSS SSA SSSSAASS SSSS     231 224            LLLLFFLFL YFFFFYFY CCTTLIL CCFF IVIVV LVLLLALL YYYYFFF FFFFFFFFFF            FFFFFF YYF FFFFFFFF FYYF     232 225            GGGGGGGGG GGGGGGGG GGGGGGG GGGG GGGGG GGGGGGGG GGGGGGG GGGGGGGGGG            GGGGGG GGG GGGGGGGG GGGG     233 226            VVVVIIVVI VVVVVVVV VVVVVVV VVVI CCTCV CVLLILII IVVVIII IIIIIIIIIV            VVVIII VVV VVVIVVVV IIIV     234 227            LLLLLLLLL LLLLLLLL IIIIIII IIVV IIIII MIMSTVIL VVVVTTT LLLLLLLLLL            LLLLLL TTT VVVLLLVV LLFL     235 228            IIIIIIIIM LLLLLLLL LLMMTCA LLLL FFFFF LLIIVILL LLLLLLL LQLLLLLLLM            LLLLLL VVI LLLIVCLL LLLL     236 229            YYYYYYYYY YYYYYYYY YYYYYYY YYFY AAAAA ALILFFIY YYYYWWW TTTTYTTMMW            WWWWWW WWW WWWWWWWW WWWW     237 230            EEEEEEEEE EEEEEEEE IITTMII VSVV EEEEE SSEEEEND EEEEQQQ EEEEEEEEEE            EEEEEE EEE EEEEEEEE EEEE     238 231            MMMMMMMML MMMMMMMM LLLLLLL MLLL MMLML MLLMATLM LLLLMMM LLLLIIIILI            IIITTA LLL IIMITIIL IILI     239 232            AAAALLLLL LLLLLLLL LLLLLVL LLVV CIAFM ILVAAACV MMMMTTT TVIVVVVVFF            AAAFFF MML TAALLLFM FFFV     240 233            AAAAAAAAT AAAAAAAV VVAASSS CSCC NRTRL FGTLAVCC TTTTTTT TTTTTTTTTT            TTSSSS TTT STTTTTTT TSST     241     ------ KKKKYHHYYC YYYLLL FFF LLLLLLYR LLLL     242 234            GGGGGGGGG GGGGGGGG GGGGGGG RNGG RRKRR RRGGNHKG GGGGKRR GGGGGGGGGG            GGGGGG GGG AAAGGGGG GGGG     243 235            YYYYYYYYS QQQQQQQQ YYSSLLT RKKK KSKKI KRERINRD ESSSQEE RRRRKRRRQK            MMMAAA SAQ EEAHQQKA GLSG     244 236            PPPPTTTTP PAAAPPPP PPPPSSM LLVV PPPPP FFFYVANI LLLLAVV VVIVIIIIVM            SSASSV KKR QQQQQQQP TNSN     245 237            PPPPPPPPP PPPPPPPP PPPPPPP PPPP ILLLY PPPPLSPP PPPPPPP PPPPPPPPPP            PPPPPP PPP PPPPPPPP PPPP     246 238            FFFFFFFFF FFFFFFFF FFFFFFF FFFF FFFFL FMLYPLWF YYYYYYY YYYYYYYYYY            YYYYYY YYH YYYYYYWY YYYY     247 239            FFFFYYYYS DEEEDDDD WWWWLME DGDD SPHCP FFGPDFK- SSSSSSS PPPPPPPPPG            PPPPPA DDE QQQPPAYP PPPP     248 240            AAAADDDNG GGGGGGGG DDHHGGD DGDD GGGGG HQGPNSR- HHHH--- GGGGGGGGGR            GAGNNN GGN GGGAGAQD EGGG     249     ------- - GSH-GAA- IIII---     250     ------- - HLN--PC-     251     ------- - D---R---     252     ------- - N---G---     253     ------- - ---     254     ------- -     255     ------- -     256     ------- -     257     ------- -     258     ------- -     259     ------- -     260 241            DDDDSSTSP EEEEEEEE EERRDDD EQEE DDDDD DDDEQSSE NGGGGGG MMMMRMMMML            IIVLLL III LLLHLRLV PLPI     261 242            QQQQNNTND DDDDDDDD DDKKDNN SNNN QEQQQ QDTTSQQH NCCSEEE VVNNTTTSHK            DDESSS PPP SSSSSNSN MVVP     262 243            PPPPTTPTP EEEEEEEE QQQQDDR ITSS IILLM LAPYWITD RRRRRPP NNKNNNNNSN            RLLNNN AAA NNNNNNNT NNDP     263 244            IIIIMMMMM DDDDEDEE HHMMTNT PDSS FFFGE VDDDQTDE DDDDQQQ RRRRAPPPRT            STSQQQ SRK EEELIFTF DSSE     264 245            QQQQKKKKK EEEEEEEE RKLLEER VVVI KKRKV RSGNKRGE QQQQHYH EEEEDEEEEE            QDNQQQ EED QQQDEEED QKKR     265 246            IIIITTTTT LLLLLLLL LLMMTTL LILL IIIIT ILIILITI IIIIIVV VVVVVVVVVV            VVVTTT III VVVVVVAI FFFL     266 247            YYYYYYYYY FFFFFFFF YYLLLLY FYHH FFFFF ALLFRIYI IIIILQQ LLLLMIIIIV            YYYRRR SPP LLLLLLIT YYYF     267 248            EEEEEEEEN QQQQQQQA QQRRNAR KNEE RQRDR KEDSSRRK FFFFYYY DEEETQQREE            EHGEEE SDD KRRNHADV NKKN     268 249            KKKKKKKNI SSSSASAA QQMMNNQ NKKK VVALA VLLQGQSG MMMMAAA QQQQANNANR            LKLFFA ILL FFYYHHCY ALML     269 250            IIIIIIIII IIIIIIII IIIIVVI IIII LLLIL LCLLDAYQ VVVVVVV VVVVLLLLIV            LLLVVI LLI VVVVVVIL IVIL     270 251            VVVVLLLLL MMMMMMMT KKMMLTL SRKK GGGGG GTQSLQVV GGGGVVV EEEESEEEEQ            EDEEEE EEE MMIQRKTL KKKK     271 252            SSSSNNQNR EEEEEEED AASDSSR NHQK TTTLT TIRASVHF RRRRAAA RRQRQRRRRR            KKNKKQ KKV DEDTSEQQ RDET     272 253            GGGGAAGAG HHHHQHQH GGGGGAG GGGG PPPPP EFIIDHNF GGGGYYY GGGGGGGGGG            DGGGGG GGG GGGGGGGG GGGG     273     --- NNNPT EGVVAVPR YYYYDNN YYYYYYYYFI YYFGGV EEL GGGGGGGR YYFH     274 254            KKKKEEKED NNNNTNTN AANKNTK VAKK EENED LWNDPDSQ ALLLLLL RRHRRRRRRI            RRRRRR RRK YLVRRRER RQRR     275 255            VVVVLLVLI VVVVVVVV YYYYWWY YYVV AEEDR YKEGRETT SSSSRRR MMMMMMMMML            MMMLLL LLL LLMLLLLL MMMM     276 256            RRRRRRKVE SAAATSTS DDQQYTS TDED IVVDD AEPPLFL- PPPPPPP PPPPPVVPPE            KEDPPE PPE DDEEEQEL AALE     277 257            FFFFFFYFF YYYYYYYY FFFFFFY LLYY WWWWW YLSPSSL- DDDDSSH CCCCRRRRKK            RRGCCP QQQ QKRPSQRQ QQSR     278 258            PPPPPPPPP PPPPPPPP PPGGDDS PPPP PPPPP LRPPSPS- LLLLLLW PPPPVPPPPP            PPPPPP PPP PPPPPPPP PPPP     279 259            SSSSPPPPK KKKKKKKK SSSSEDG KSQS DGERE DKRRTHI- SSSSSAQ PQPQEDDETK            EPQEEE PPE DDERNPRE AAED     280 260            HHHHFFYFK SSSSSSSS PPPPEEE FSHH IVVDV KCLLDPL- KKKKAG- EGGDNNNNNS            GGGLLQ III NNNNNMAY HFHN     281     ------- - YAPPNEP- LIIIAAA     282     ------- - NAKSGS-- YSSFVVV     283     ------- - ILDDSR-- KSSSFFF     284     ------- - DHRKSL-- NNNNETT     285     ------- - LGI-LT--     286 261            FFFFFFFFI LMMMLLLL VVYYVIV LILL LGLMA VDYFGMIV CCCCPTT CCCCCCCCFC            CCCCCC CCC CCCCCCCC AAAC     287 262            SSSSNNQHA SSSSSSSS TTSSSSS SSSS DEDES SHSSQDSS PPPPGGG PPPPPPPPPA            PPPPPP TTS PPPPPTPP SPPS     288 263            SSSSEEPPK KKKKRKRK PPDDDDN PSII PEEEE PYKSGIRS KKKKQKK EEAIDEEEDK            EPPDDE IIL EDDDDEPD DKAE     289 264            DDDDDDDDN EEEEEEEE EETTEDL GAEE RDNSY EWEDGDEE AAAARTT SSSSEEEENE            KESAAD DDD RMFDDKEP ENEE     290 265            LLLLVVVAA AAAAAAAA AAVVAAA AAVV GAGGA ACMALVLC MMMMLLL LLLLLLLLII            VVVVVV VVI VLLLILVL IIMM     291 266            KKKKKKVQA VVVVVVVK KKKKKKK AQII IILAL LFTQDENQ KRRRGQQ HHYHYYYYYY            YYYFFY YYY TFHWRYYY YYYY     292 267            DDDDDDDDN SAAAASAE DNDDDDD GDSS DEDQD DQDDRYSH RRRRDNN DEEEDQHNQD            EDRRRR MMC DEKNDSAE ESDR     293 268            LLLLLLLLL IIIIIIIA LLLLFFF LLLL LLLLF FVFFVLLL LLLLVIN LIALILLILV            LLLLLL IIT LLLLLLIV IIIL     294 269            LLLLLLLLI CCCCCCCC IIVVVII ILLL LLLLM LLVVVVLI VLLLIIV MMMMMMMMLM            MMMMMM MML MMMMMLMM MMMM     295 270            RRRRSSSKK KKKKKKKK NNSSSSD KHST DSSLC DENSECNK ASSTQQQ CNEIKRMMLK            RRLEEQ VVL RRQTTLRL QQKL     296 271            NNNNRRKKK GGGGGGGG KQRRNNR RRKR KAKEG KQRLQKRW DDDDRSS QLQHMLLRQL            AQQQQR KKS MMRQRLGK KATQ     297 272            LLLLLLLLL LLLLFLFF MMFFLLL MMMM LMMMM LCCCMAIC CCCCCCC CCTCCCCCCC            CCCCCC CCC CCCCCCCC CCCC     298 273            LLLLIIIIC MIMMLMLL LLLLILL LLLI LLLLL LFCLLLFL VLLLWWW WWWWWWWWWW            WWWWWW WWW WWWWWWWW WWWW     299 274            QQQQTTTTR TTTTTTTT TTVVVKT IDVV AVITT REIQSTDS KKKKREE RKRKKKKKDS            QQNAAE MMH QQHAARQH EADK     300 275            VVVVRRARD KKKKKKKK IIVVKKV VVVV YYYFM YMKKPFRL KFFFPAA KKLKEEENAH            WWWYYY IIL FYHQQTRP ELAQ     301 276            DDDDDDDDN HHHHHHHQ NNQQEDD NNDD DDDNN DDNIEDNR VQQQSRR EDDDKRRRVG            NDSEED DDD NNREDDEK KEDE     302 277            LLLLLLLLP PPPPPPPP PPPPQMP PPPP PPPPP HPEPPGPP KRRRAAA PPPPPPPPPP            PAPPPP ASA PPSPPPPA FPPP     303 278            TTTTSSTSS GGGGAAGN SAQQGKG LSKL IAAHQ VQRERAKS EEEEALL EDEEEEEEEE            STSGGR DEA NKSDHWQE ETLD     304 279            KKKKQQREE KKKKKKKK KKKDASA NTRR NHKKK DKERNLTD EEEEQQQ EEEEEDDEKE            DDDQQR SCM MMAQNEQM THKK     305 280            RRRRRRRRR RRRRRRRR RRRRRRR RRRR RRRRR RRRRRRRR RRRRRRR RRRRRRRRRR            RRRRRR RRR RRRRRRRR RRRR     306 281            FFFFLLILL LLLLLLLL IIYCMLM IIAA IIIIW LSSPPPIP PPPPPPP PPPPPPPPPP            PPPPPP PPP PPPPPPPP PPPP     307 282            GGGGGGGGG GGGGGGGG TTTSSNT STTT SSSST TSSTTSTS LLLLSGG TTTTTTTTTA            STRSSS KRT TSSTTSSS PTTV     308 283            NNNNNNNNN CCCCSCSC AAAAACA IILL AAGAA AAIYIALF FFFFAAA FFFFFFFFFF            FFFFFF FFF FFFFFFIF FFFF     309 284            LLLLLLLLL GGGGGGGG AHEEATL HPKK RKKFV REHADAPE PPPPREE EEEEDDDEER            AKRSSG RRK LLLHFRKS SQKA     310 285            KKKKQQQQK PPPPPPPS EEEEQQQ EEQN RRMRQ EDEAQEEE QQQQLLL YYYYYYYYFV            ESDTAA EEQ EEDRYRDE QQQD     311 286            DDNNNNDNN EEEEDEDS AAAACCA IFVV AAAAC ALLLILLI IIIILLL LILLLLLILL            IIIIIV LLL IIIIICVL LIII     312 287            GGGGGGGGG GGGGGGGG LLLLLLL MFVV ALLLL MLLTLLSR LLLLLQQ QQQQQRRQNM            HHHYYH IVT VIIQQYHV VCVS     313 288            VVVVTTSSV EEEEEEEE KKAAAQR QSEE IQNQE AKHEACTN SAAAVRK ASSSSSSSHD            QHFQQQ IST NSADHNAS LSQK     314 289     ---T--- STTTDDD FFFFVVVVYQ AANEED EEV LSYQKTRR LFLD     315 290     ---D--- IIIILLL LLLLLLLLFL FLLLLL FFF LILLLLLI LLIL     316 291     ------- - IVVIVI VIIIIVIV     317 292     ------- - KKKKKQ RKKKRRRR     318 293     ------- - TNNANK EEDEAEAL     319 294            HHHHHHHHH HHHHHHHH HHHHHHH DHHH HQHHS HTHHELLH EEEETKK EEEEDEDDEA            EEEQQI SSA KKEQQHQS EQEE     320 295            KKKKPPPPK AAAAGAGP PPPPPPP DPHP PNPSD PPDPVPVP LLLLSAA DDDDDDDDSL            THNSSA KRE DEPLEAAA REKK     321 296            WWWWWWWWW FFFFFFFF WWFFWWW WFWW YYYYY YFLWCLSW LLLLLFF YYYYFFFFFV            MMLIII MMF DEQFIILI LQQM     322 297            FFFFFFFFF FFFFFFFS IVFFLLV FLMM FLFLF FFILWFNM QQQQKRR FFFFYFFYSA            FFIRRR AAA LMCRRSAF LAIM     323 298            AAAAKKSNE RRRRRRRR SCQQNQV KMVN QRNHK LNMVVQCQ HRRRAGG TTTTTTTTTQ            QQSKKK RRR HEPNHTQS GQSV     324 299            TTTTEEEEG RYYYWRNR HQQENKS VGRR EDDKE PEKKEQKG SSSSEAA SASAAAAATT            EVSRRR DDD PPNFSDAT EEEK     325 300            TTTTVVVVF IIIIIIII RRYYLDM DCGG SFLDL ILTTMKND LLLLLLL TTATTTTTSL            SGNHHH PPP SGSFPLPF GDSR     __________________________________________________________________________

    TABLE 9       -  02 03 04 05 06 07 08 09 11 12 13 14 15 16 17 18 20 21 22 23 24 25 26 2      8 29 30 31 33 34 35 36 37 39 40 41 42 43 44 45 46       01 99 95 95 60 62 65 62 56 51 52 52 51 50 51 50 50 42 42 43 42 38 41 44      44 44 43 43 33 34 36 31 34 32 26 33 38 34 31 34 36       02  95 95 60 62 65 62 56 51 52 52 51 50 51 50 51 42 41 43 42 38 41 44      44 44 42 43 33 34 36 31 33 32 26 33 37 34 31 34 36       03   99 60 62 66 62 57 52 53 53 51 50 52 50 51 42 41 44 43 39 41 46 44      45 44 44 33 34 37 31 33 32 26 34 38 34 31 34 36       04    60 62 66 62 57 52 53 53 51 50 52 50 51 42 41 44 44 38 41 46 44 44      44 44 33 34 37 31 33 32 26 34 37 34 31 34 36       05     96 79 87 52 48 49 49 48 47 48 46 48 38 37 40 40 38 40 42 44 43      41 42 33 33 33 31 33 31 27 33 35 34 32 35 36       06      83 91 54 50 51 51 50 49 50 48 50 39 38 40 40 39 41 43 44 44 42      43 33 32 34 32 33 31 28 34 36 34 32 36 36       07       82 53 53 54 54 53 52 53 52 52 41 40 41 42 39 41 45 45 42 43 43      33 32 33 33 33 32 27 35 38 34 32 34 36       08        55 51 53 52 51 50 51 50 52 40 39 40 40 39 41 44 46 44 42 42      34 31 33 34 33 32 29 34 36 34 33 35 36       09         48 48 48 48 46 48 46 48 38 38 38 38 37 40 41 42 41 38 38 32      31 33 30 32 31 28 36 34 35 27 32 37       11          91 92 89 83 99 83 84 41 41 38 38 39 40 42 44 41 44 45 30 31      33 30 34 33 27 33 35 34 28 34 36       12           99 96 83 91 83 82 40 40 38 37 38 39 42 43 40 42 43 29 30      32 30 33 33 26 33 35 34 29 34 35       13            97 83 91 83 82 40 40 38 37 38 39 42 43 40 42 43 29 30 32      30 33 33 26 34 35 34 29 34 35       14             83 89 83 79 38 38 36 36 38 38 40 42 39 42 42 28 31 31 29      32 33 26 33 34 34 28 32 34       15              83 98 78 38 38 37 37 35 37 40 43 39 42 43 29 31 31 30      33 31 25 31 34 33 27 31 37       16               83 84 41 41 38 38 38 39 41 44 41 44 44 30 32 33 30 34      33 27 33 35 34 28 34 36       17                78 38 38 36 36 35 36 41 42 39 42 42 29 31 31 29 32 31      25 31 34 33 27 31 36       18                 42 42 39 38 39 39 42 45 41 42 43 31 32 34 32 35 32      27 32 36 34 27 33 37       20                  93 48 47 45 44 50 50 49 42 42 36 37 42 37 38 35 31      34 34 34 35 37 38       21                   48 47 44 44 50 50 48 41 41 37 38 41 47 38 34 33 33      34 34 34 38 38       22                    94 44 44 46 44 43 42 41 34 34 37 37 36 34 30 36      37 35 33 35 38       23                     43 43 46 43 42 41 40 35 35 37 36 36 35 30 36 37      34 33 34 39       24                      62 47 42 42 37 36 34 34 37 32 37 34 30 35 36 33      29 33 37       25                       46 44 43 41 40 34 34 37 33 37 37 31 34 38 34      32 34 38       26                        46 44 42 42 34 33 37 33 39 35 30 34 37 37 34      36 36       28                         54 52 51 40 39 40 38 39 35 32 36 35 35 33 41      42       29                          48 48 35 35 39 36 38 35 31 35 35 35 35 43      40       30                           89 34 33 37 36 34 35 27 34 37 34 33 38 38        31                            34 33 36 36 33 34 27 34 36 34 32 37 38         33                             69 67 50 47 38 34 34 33 34 34 33 31          34                              70 52 48 36 33 34 36 33 33 34 33           35                               51 52 38 34 38 35 34 36 37 33            36                                44 33 31 35 32 32 34 36 31             37                                 36 34 34 34 32 34 32 32              39                                  33 30 30 32 30 30 32               40                                   30 27 27 30 30 30       41                                    47 33 32 37 32       42                                     34 31 35 34       43                                      30 33 31       44                                       31 30       45                                        37       46        48 49 50 51 52 53 54 56 57 58 59 60 61 62 63 64 65 67 68 69 70 71 72      74 75 76 78 79 80 81 82 83 84 85 87 88 89 90       01 32 33 32 33 30 30 29 35 35 34 36 34 33 34 33 36 34 37 38 35 35 34 36      34 33 35 31 31 30 30 31 30 32 32 33 31 33 34       02 32 33 32 33 30 30 39 35 35 34 36 34 33 34 33 36 34 37 39 35 35 34 36      33 33 35 32 32 31 30 31 31 32 32 33 31 33 34       03 33 33 32 33 30 30 30 35 35 34 35 34 34 34 33 36 34 38 39 35 35 35      373        34 34 35 32 33 31 30 31 31 33 31 32 32 34 34      04 33 33 32 33 30 30 29 35 35 34 36 34 34 34 33 36 34 38 39 35 36 36 373       34 34 35 33 33 31 30 31 31 33 31 32 31 33 33       05 33 35 35 34 32 30 30 34 35 35 35 34 34 34 34 36 34 36 35 34 37 37 38      34 32 33 33 30 32 32 31 33 33 34 34 34 35 34       06 34 35 35 35 32 30 31 34 35 35 35 34 34 34 34 36 34 37 36 34 38 38 39      33 32 33 34 31 32 32 31 33 33 33 34 34 35 33       07 34 36 36 35 29 30 30 35 36 37 36 35 35 35 34 36 34 37 37 35 36 35 37      34 34 33 32 31 31 31 30 32 34 34 33 34 34 35       08 34 35 35 35 31 30 31 35 35 37 36 34 33 34 33 36 34 37 36 34 37 37 38      34 34 33 33 31 32 32 31 33 34 35 33 34 34 33       09 34 33 33 33 30 32 31 36 37 38 38 36 35 35 35 37 35 36 35 35 35 35 35      33 31 32 32 31 31 32 30 33 32 33 34 36 36 32       11 33 33 33 33 29 30 30 36 36 35 37 35 35 36 36 34 36 37 36 35 34 34 35      34 34 32 32 31 30 29 30 39 34 32 33 34 35 33       12 33 33 33 33 29 30 30 36 35 36 37 36 36 36 36 35 36 38 37 35 36 35 36      33 33 33 33 31 31 29 30 30 35 33 34 34 35 33       13 33 33 33 33 29 30 30 36 35 36 37 36 36 36 36 35 36 38 37 35 36 35 36      33 33 33 33 31 31 29 30 30 35 33 34 34 35 33       14 32 32 31 32 28 29 30 35 35 35 36 35 35 35 35 35 34 35 35 34 35 34 35      31 32 32 32 31 30 28 28 28 34 32 34 34 35 32       15 31 31 31 31 29 29 29 34 34 34 34 34 34 35 35 34 33 33 33 33 34 33 34      32 32 31 32 32 30 27 28 28 33 30 31 31 33 33       16 33 33 33 33 29 30 30 36 35 35 36 34 35 35 35 34 36 37 37 34 34 34 35      34 34 32 32 31 31 29 30 30 34 32 33 34 34 33       17 31 31 31 31 29 29 29 34 34 34 34 34 34 34 34 34 33 34 34 33 34 33 34      32 31 31 32 31 30 27 28 28 33 30 31 31 33 32       18 33 33 33 33 29 30 30 36 36 35 38 35 34 35 36 34 35 37 36 34 36 35 36      35 34 33 34 33 32 29 30 31 36 32 33 34 35 34       20 32 34 34 34 30 30 30 34 34 34 34 34 34 34 34 33 35 35 35 35 34 33 34      32 32 33 31 31 30 31 31 33 31 33 32 31 32 34       21 33 34 34 33 30 30 31 34 33 34 34 33 33 33 33 34 34 34 34 34 34 34 34      30 31 33 30 30 29 30 30 32 31 33 31 31 31 32       22 34 34 34 34 29 28 29 33 32 31 32 31 30 31 31 33 31 32 32 29 33 33 34      31 31 31 29 28 30 28 29 27 30 30 29 29 30 30       23 33 34 33 33 29 28 28 32 32 30 31 30 30 31 30 32 30 33 32 30 33 32 34      31 30 30 30 28 30 28 28 28 30 30 29 29 30 30       24 33 34 34 33 32 31 32 39 38 37 38 37 38 38 37 35 36 36 36 34 35 35 34      31 32 32 30 30 31 33 33 33 31 33 34 34 34 34       25 34 33 33 33 33 32 32 38 38 36 38 35 36 37 36 35 34 34 34 33 35 35 36      31 32 31 31 30 30 31 32 32 32 32 32 32 32 32       26 34 34 35 34 31 30 29 34 33 34 33 31 30 30 31 33 34 31 33 31 34 34 35      31 31 33 32 30 31 31 32 32 31 30 31 31 33 32       28 34 35 34 35 32 33 33 37 37 37 37 38 38 38 37 36 35 37 35 36 36 36 37      34 33 33 34 34 34 31 31 31 36 34 34 35 35 37       29 35 36 35 35 31 31 31 38 38 38 38 38 37 38 37 38 37 37 37 37 36 36 37      35 34 36 35 34 33 33 33 33 34 34 35 36 36 34       30 34 35 34 34 32 31 32 36 36 36 37 37 37 37 35 36 36 37 39 37 38 38 40      35 34 34 35 35 34 31 32 32 39 36 36 35 37 36       31 33 33 32 33 33 31 33 35 35 34 36 35 36 36 35 36 34 35 37 35 37 37 39      34 33 32 34 34 33 31 31 31 37 34 34 34 36 34       33 29 28 28 28 32 30 30 31 31 31 31 30 31 31 30 31 31 32 33 32 32 32 32      32 31 20 31 30 31 29 30 30 32 31 29 30 30 32       34 29 29 29 28 31 30 30 30 31 30 30 30 32 32 30 31 30 33 33 33 33 33 34      31 31 29 28 29 28 28 26 29 29 30 30 30 31 30       35 31 32 32 31 31 30 30 34 34 33 33 33 34 35 34 33 33 35 37 34 34 35 36      32 31 32 31 33 31 30 29 32 31 32 30 29 31 33       36 31 31 31 30 30 29 28 30 29 29 30 27 29 30 28 30 29 30 30 32 32 32 32      28 29 27 30 28 27 27 26 29 30 29 29 28 29 30       37 31 31 31 30 30 30 30 33 33 33 33 32 32 32 33 30 31 32 32 32 31 31 31      31 31 30 30 29 29 28 28 26 29 32 29 30 31 31       39 28 29 29 19 28 26 26 29 29 29 29 28 29 29 28 29 27 28 30 28 28 28 28      26 27 25 28 26 25 24 24 25 26 27 27 26 27 27       40 27 26 26 26 29 28 28 28 27 29 29 27 29 28 27 30 28 26 28 26 29 29 28      26 26 27 25 24 24 25 23 25 27 28 26 26 27 29       41 36 34 34 34 35 34 35 34 34 32 34 34 33 34 33 36 34 34 32 31 33 34 33      30 31 30 29 29 29 28 27 29 31 30 35 32 33 34       42 36 36 36 35 33 32 32 36 35 34 36 35 36 37 34 37 34 34 34 34 34 34 35      35 35 31 31 31 30 32 31 31 32 31 33 33 34 36       43 36 34 34 34 33 33 32 34 36 34 35 35 35 35 34 35 31 32 33 32 35 35 36      30 30 29 32 31 32 31 31 31 33 30 35 36 36 34       44 31 31 32 31 27 26 26 30 30 30 30 28 29 30 28 29 29 31 30 30 29 29 29      29 28 27 27 26 26 27 27 26 30 29 27 26 27 26       45 33 32 31 31 27 27 28 33 32 32 33 32 33 34 32 32 31 33 33 32 32 32 31      31 32 32 29 29 27 29 29 29 30 33 32 32 32 30       46 31 30 30 30 31 33 33 33 33 33 34 34 36 36 34 34 31 34 33 32 34 34 34      32 32 31 30 30 32 29 29 31 32 32 31 32 32 33        48 49 50 51 52 53 54 56 57 58 59 60 61 62 63 64 65 67 68 69 70 71 72      74 75 76 78 79 80 81 82 83 84 85 87 88 89 90       48  83 82 83 35 35 34 43 44 44 44 42 41 42 42 44 42 39 39 40 39 40 40      37 38 36 37 37 35 36 35 34 37 38 40 48 39 38       49   99 98 36 35 34 42 42 42 43 42 41 41 41 43 40 40 40 41 40 40 40 38      38 36 36 36 36 36 36 35 37 38 39 38 40 38       50    97 36 35 34 42 43 43 43 42 41 41 41 43 41 40 40 41 40 41 40 38 38      36 36 36 37 36 36 35 37 38 39 39 40 38       51     35 34 34 42 42 42 42 42 40 40 41 43 40 40 40 41 40 40 40 38 38      36 35 36 36 36 36 35 37 37 38 38 39 38       52      81 79 38 36 37 37 38 37 38 39 35 34 32 33 33 35 35 37 30 30 32      33 34 33 31 31 34 32 32 34 32 32 32       53       93 38 38 38 38 39 38 38 40 35 34 33 34 34 35 35 37 30 30 32 31      33 31 34 31 33 32 32 35 33 32 32       54        37 37 37 37 38 37 38 40 35 33 32 34 34 35 35 36 30 30 32 31      33 31 34 31 33 31 32 34 32 31 33       56         91 83 87 73 72 74 75 66 58 58 60 56 53 53 50 46 49 46 48 47      46 50 50 48 46 46 47 47 46 47       57          83 90 74 73 75 77 66 57 59 59 57 53 53 52 46 48 46 47 47 45      51 50 48 45 45 46 48 46 47       58           83 72 69 70 72 65 58 57 58 57 50 50 49 45 48 46 47 46 43      48 47 47 46 46 47 47 47 45       59            75 74 76 76 65 58 57 58 56 52 52 52 46 49 45 46 46 44 50      48 47 46 46 47 48 47 46       60             77 80 86 62 57 58 59 57 50 50 51 45 46 46 47 47 44 50 48      46 48 48 50 49 48 47       61              94 79 60 56 56 58 57 51 51 50 45 46 45 46 46 43 49 47      46 47 45 49 48 47 50       62               82 61 58 58 59 58 53 52 52 45 47 45 46 46 43 50 48 47      47 46 48 48 46 49       63                61 56 57 58 57 50 50 50 45 46 45 47 46 44 50 49 45 47      44 49 50 50 48       64                 57 58 58 57 56 56 55 45 49 48 46 45 45 47 48 47 46      48 49 48 48 48       65                  57 59 56 52 53 52 45 48 46 45 44 44 49 48 48 52 47      48 47 45 46       67                   81 71 53 53 52 50 50 49 50 51 49 49 49 47 48 50 47      48 48 50       68                    72 53 53 52 49 50 50 47 48 46 50 49 47 52 49 48      47 48 49       69                     52 52 51 48 49 48 49 50 49 49 49 49 48 48 47 48      48 49       70                      98 84 43 45 42 50 50 48 51 50 48 50 48 46 49 47      48       71                       83 43 45 42 50 50 48 51 50 48 50 48 47 49 47      48       72                        43 44 43 50 49 48 49 48 49 50 49 46 46 46 48        74                         85 64 44 44 43 45 46 47 44 49 44 44 44 48         75                          65 45 44 44 45 47 47 45 50 46 45 45 48          76                           41 42 41 45 46 45 42 47 44 44 44 43           78                            84 70 46 57 54 53 49 47 49 50 51            79                             71 56 56 56 52 49 46 47 46 52             80                              52 54 51 51 49 44 46 45 51              81                               78 61 50 48 48 50 47 52               82                                62 49 47 47 51 48 50       83                                 49 46 47 47 44 47       84                                  46 50 51 50 50       85                                   48 46 48 47       87                                    69 67 54       88                                     76 56       89                                      57

                                      TABLE 10     __________________________________________________________________________     Positional Surface, Parsing, Inside, and Active Site Assignments for     Protein Kinase     Number of Variable Subgroups     ≧10  9     8   7     6     5   4     3     2     __________________________________________________________________________     001        11.5.8   4.8             7.5   9.4; 8.4     002               5.7 6.6; 4.6                    9.2; 8.2; 7.2     003        INSIDE     004        11.6.6         4.6 7.4             8.3     005                   4.2         5.2     006        13.6.4   4.4   8.3     007        10.5.9   4.8   6.7                 7.3     008                         5.5; 4.6  8.4; 7.4; 6.4     009        INSIDE     010        secondary parse     011        10.4.5     012        secondary parse     013                               4.2     014        INSIDE     015     016               4.6             5.4       6.3   7.2     017        INSIDE     018                         4.2     019                         4.6             5.2     020        INSIDE     021        10.5.6             6.4; 4.5                                 8.3; 7.3        9.2     022        inside   4.2     023        10.6.7         4.6 7.6         8.4       9.2     024         5.7       6.5; 4.6                                 7.4   8.3     025        PRIMARY PARSE                            5.2; 4.3     026        PRIMARY PARSE                            4.2     027        PRIMARY PARSE                      6.2; 5.2                                                 4.2     028        PRIMARY PARSE                      6.4; 5.4                                                 4.3   8.2; 7.2     029        PRIMARY PARSE                            5.2   4.2; 6.2     030        PRIMARY PARSE     031        PRIMARY PARSE     032        PRIMARY PARSE     033        PRIMARY PARSE     034        PRIMARY PARSE     035        PRIMARY PARSE     036        PRIMARY PARSE     037        PRIMARY PARSE     038        PRIMARY PARSE     039                   5.2   4.4     040         5.7   6   7.4; 4.5                                 8.4       9.3     041        10.5.6         6.5 4.6             7.3   9.2; 8.2     042         6.4; 5.5  4.5     043        INSIDE     044        INSIDE     045        INSIDE     046        Active Site     047               4.3     048        INSIDE     049                               4.5 5.4     050               4.6       5.4     051               4.6       5.3   6.3 8.5; 7.2     052               5.3; 4.3                           6.2     053         4.6   5.2             6.2     054               4.5 5.2         6.3     055        10.4.7         5.5       6.4   7.3       8.2     056        PRIMARY PARSE                            4.2     057        PRIMARY PARSE     058        PRIMARY PARSE     059        PRIMARY PARSE                            4.2     060                   4.5         5.2     061               5.4; 4.6        6.2     062                   5.5; 4.5                                 6.4   7.4     063                   4.6         5.5 7.3; 6.4                                                 8.2   9.2     064        INSIDE     065         4.3             5.3     066               5.7       4.6       7.3; 6.3    8.2     067        Active Site     068        INSIDE     069               4.6       5.4       6.3   7.2   8.2     070        INSIDE     071        INSIDE     072                         4.6                   7.2; 6.2; 5.2     073               5.4; 4.5  6.3       7.2     074        INSIDE     075        PRIMARY PARSE                      4.3         6.2; 5.2     076        PRIMARY PARSE                                  6.2; 5.2; 4.2     077        PRIMARY PARSE     078        PRIMARY PARSE     079        PRIMARY PARSE     080        PRIMARY PARSE     081        12.6.12  4.9   8.7; 7.8                  9.3     082                               5.2; 4.2                                           6.2     083        11.5.6         6.4; 4.7     084                         4.6       5.4   6.2     085        INSIDE     086        INSIDE     087                   5.6; 4.6    6.2 8.2; 7.2     088        INSIDE     089        INSIDE     090        inside                             4.2     091        INSIDE     092        INSIDE     093        INSIDE     094        10.5.7             6.5             7.3   8.2     095                   4.7   5.5       6.2     096        PRIMARY PARSE                            4.2     097        PRIMARY PARSE     098        PRIMARY PARSE                            4.3     099        PRIMARY PARSE     100        PRIMARY PARSE     101                   4.5         5.2 6.2         7.2     102                   4.5   5.4       6.3   7.2     103        INSIDE     104        INSIDE     105        inside                                         9.2     106        INSIDE     107        INSIDE     108                                               4.2     109        INSIDE     110        INSIDE     111               6.6; 5.7  7.3; 4.6  8.2     112               5.4 6.4   7.3; 4.3  8.2     113        PRIMARY PARSE     114        PRIMARY PARSE     115                                   4.4         6.2; 5.2     116        INSIDE     117                               4.3     118                   6.4; 5.4                                 4.6     119        INSIDE     120        INSIDE     121                         4.5       5.3         6.2     122         5.7   4.8 6.5             7.3   8.2   9.2     123               5.3; 4.5                           6.3         7.2             8.2     124         4.7   6.6; 5.6  7.5             8.3   9.2     125        10.5.8   6.8; 4.8        7.4   8.3       9.2     126        11.5.7         7.5             8.3     127                         6.3; 5.3                                       4.2       7.2     128        PRIMARY PARSE     129        PRIMARY PARSE     130        12.5.7   7.6; 4.7; 6.7                           8.4             9.3     131        INSIDE     132         5.5; 4.7  6.3     133        11.5.6         6.4; 4.5                           7.4   8.4     134        INSIDE     135        10.5.2   6.2       4.2     136               5.5 4.6         6.2     137        INSIDE     138        INSIDE     139                   4.4     140                                   4.3         5.2     141        INSIDE     142        INSIDE     143                   4.7   5.5       6.3     144     145        INSIDE     146         4.8   5.6             6.4       7.2     147        INSIDE     148        INSIDE     149                                         4.2     150                   4.6   5.5   6.4 7.3         8.2     151               5.7 7.7; 6.7; 4.6                                       8.5             9.2     152                   4.7   5.5       6.3     153        INSIDE     154        INSIDE     155        INSIDE     156     157        Active Site     158        INSIDE     159     160        secondary parse     161                                   4.3     162        Active Site     163        INSIDE     164        INSIDE     165        INSIDE     166                   4.5   5.2     167        11.6.8   7.6   4.6       8.4     168        10.5.9         4.7       6.6       7.4   8.3   9.2     169                   5.5; 4.5                                 6.4             7.2     170        PRIMARY PARSE     171        PRIMARY PARSE     172        PRIMARY PARSE     173        PRIMARY PARSE     174        PRIMARY PARSE     175        PRIMARY PARSE     176        PRIMARY PARSE     177               4.3     178        INSIDE     179        Inside                                         5.2; 4.2     180        INSIDE     181        INSIDE     182        Active Site     183        INSIDE     184        secondary parse     185        INSIDE     186        INSIDE     187                                   4.4         7.2; 6.2; 5.2     188                               6.2; 5.2                                           4.3         7.2     189                   4.2     190        12.5.10  4.6                   8.4       9.2     191         4.5   5.3 6.3         7.2     192                   5.7; 4.6        7.4; 6.4    8.2     193         4.7             5.4       6.2   7.2     194                         4.5       5.3   7.2; 6.2     195        PRIMARY PARSE                  4.3 6.3; 5.3                                                 7.2     196        PRIMARY PARSE     197                   4.4   5.2     198                   4.4   6.3; 5.3  7.2   8.2     199                         4.3     200               4.4       5.2     201                   4.3             5.2     202        INSIDE     203        secondary parse     204        secondary parse     205                                         4.2     206        INSIDE     207        INSIDE     208        INSIDE     209        secondary parse     210        Active Site     211        INSIDE     212        INSIDE     213        PRIMARY PARSE                                  4.2     214        PRIMARY PARSE     215        PRIMARY PARSE     216        PRIMARY PARSE     217                   5.4   6.3; 4.5     218               4.6 5.3   6.3             7.2     219               4.7             5.4 6.3         9.2; 8.2; 7.2     220        10.5.4   4.4   6.4     221        INSIDE     222                                               9.2     223                   4.3   5.2     224        PRIMARY PARSE     225               4.7 5.3     226        inside     227        CM1D     228        INSIDE     229        INSIDE     230        INSIDE     231        INSIDE     232        secondary parse     233        INSIDE     234        INSIDE     235        INSIDE     236        INSIDE     237     238        INSIDE     239        INSIDE     240        INSIDE     241        PRIMARY PARSE                                  4.2     242                                   4.3         6.2; 5.2     243         4.7   6.5; 5.5            7.3     244               5.2 4.2     245        secondary parse     246        INSIDE     247                   5.2; 4.2     248                         4.5   5.3 6.2     249        PRIMARY PARSE     250        PRIMARY PARSE     251        PRIMARY PARSE     252        PRIMARY PARSE     253        PRIMARY PARSE     254        PRIMARY PARSE     255        PRIMARY PARSE     256        PRIMARY PARSE     257        PRIMARY PARSE     258        PRIMARY PARSE     259        PRIMARY PARSE     260                         6.2; 5.4; 4.5   7.2     261               5.7 4.6   6.5       7.3     262                   4.5   6.3; 5.3  7.2     263               5.5 4.6   6.4       7.2     264        10.5.8   4.7   6.6             7.3 8.2     265        INSIDE     266        INSIDE     267         5.8   6.7; 4.8  7.5       8.4     268        10.6.3             4.2         7.2     269        INSIDE     270                   5.3         6.3; 4.3  7.2     271        11.5.11        7.8; 4.7        8.5             9.2     272     273                   4.4   5.2     274        10.5.8   4.8   6.6       7.5     275                               4.2     276        12.5.7   6.5   4.7             7.4 8.3     277               6.3; 5.3  4.3     278        secondary parse     279        11.5.8   4.7   6.6     280                   5.2   4.5     281        PRIMARY PARSE                  4.2     282        PRIMARY PARSE     283        PRIMARY PARSE                            4.3   5.2     284        PRIMARY PARSE                  4.4       5.2     285        PRIMARY PARSE                            4.2     286        INSIDE     287                         4.2   5.2       6.2     288        11.5.10  6.9   7.8; 4.7        8.5     289         5.9   4.8 6.7             7.4         8.2     290        INSIDE     291         6.4; 5.4  7.3; 4.3     292        10.5.9   6.9   7.7 4.6   8.4       9.2     293        INSIDE     294        INSIDE     295        11.5.7   6.7; 4.6  8.5; 7.5        9.2     296        12.5.11  4.9   7.6       8.5             9.2     297        INSIDE     298        INSIDE     299        11.5.7   4.6       6.5                   7.3   8.2     300               5.4; 4.5  7.4; 6.4     301        10.5.8   6.7; 4.8  7.5                   8.3     302               4.2     303        14.5.9             8.5         9.2     304        10.5.7   6.7   4.6                 8.3; 7.3     305        Active site     306        secondary parse     307               5.3; 4.3  6.2     308        INSIDE     309        10.5.7   4.7   6.5             7.3     310        10.5.7         4.8 6.5             7.2     311                         7.3; 6.3; 5.2     312        INSIDE     313        11.5.4             7.2         8.2     314        10.6.6                   7.4; 4.5        8.2     315        PRIMARY PARSE     316        PRIMARY PARSE     317        PRIMARY PARSE     318        PRIMARY PARSE                      7.4; 6.4                                                 9.3; 8.3; 5.3                                                       4.2     319               5.4 4.5   6.4             7.2     320        12.5.8   4.7       7.2             8.2     321        INSIDE     322         5.2       4.3     323        11.5.7                   7.4       8.3     324        11.5.9   6.7       4.7   7.4   9.3 8.2     325               5.3       6.2; 4.5     __________________________________________________________________________

                                      TABLE 11     __________________________________________________________________________     Secondary Structure Predictions for the Protein Kinase Family.        maximum              minimum                   positions                        secondary                             max min cAMP     unit        positions              positions                   cAMP-PK                        struct.                             length                                 length                                     PK  comments     __________________________________________________________________________      1 001-012              001-011                   001-011                        coil 12  11  11  not active site (?, chem.                                         modification)      2 012-023              014-021                   012-022                        beta 12  8   11  bend positions 15-16      3 023-042              025-041                   023-041                        coil 20  0    5  not active site      4 041-049              042-048                   042-048                        beta  9  7    7  active site, bend 46      5 049-063              050-059                   049-060                        coil 15  1   10  not active site      6 060-075              064-073                   061-074                        alpha                             16  10  14  active site 67      7 074-083              075-083                   075-083                        coil 10  3    3  not active site      8 083-095              084-092                   084-093                        beta 13  9   10  bend position 87      9 093-103              096-100                   094-102                        coil 11  0    8  not active site     10 101-111              103-107                   103-111                        beta 11  5    9  bend position 108     11 108-115              112-114                   112-114                        coil  8  1    2  active site 113 & 116     12 115-125              115-122                   115-124                        alpha                             11  8   11  confirmed in subfamilies     13 123-132              126-128                   126-130                        coil 10  0    2  not active site     14 129-160              133-151                   131-152                        alpha                             32  19  22  surface helix     15 152-160              omitted                   153-156                        beta  9  0    4  weak assignment     16 152-162              161-162                   157-162                        active site                             11  2    6  active site coil     17 163-168              163-165                   163-166                        beta  6  3    4     18 166-177              169-176                   167-176                        coil 12  0    3  not active site     19 177-187              178-184                   177-186                        beta 11  8   10  active site bend 182     20 185-191              omitted                   omitted                        alpha                              7  0    0  divergent conformation     21 185-204              192-197                   187-200                        coil 20  2   10  active site coil     22 198-217              205-208                   201-212                        active site                             20  4   12  parse at 203-204 & 208-209     23 209-225              218-224                   213-225                        coil 16  5    8  not active site     24 225-243              226-240                   226-241                        alpha                             19  15  16  entirely internal     25 241-261              244-259                   242-261                        coil 20  4    8  not active site     26 260-275              263-272                   262-274                        alpha                             16  10  13  realign alignment     27 273-288              276-284                   275-285                        coil 16  0    6  not active site     28 284-301              288-300                   286-301                        alpha                             18  13  16     29 301-313              302-306                   302-306                        coil 13  5    5  active site (?) coil     30 307-313              omitted                   307-312                        beta  7  0    6  weak assignment     31 313-318              314-318                   313-318                        coil  6  1    6  not active site     32  319-325+              319-325                   319-325                        coil  7+ 7    7+ possible short helix     __________________________________________________________________________

                  TABLE 12     ______________________________________     Orientation of the Helix Between Positions 115 and 122 in Protein Kinase     functional    outside-inside                              inside-outside     subgroup            MPI    border     border     ______________________________________     1      50%    115-119    117-121     2      80%    119-123    118-122  largely buried?                                       MPI = 80%     3      45%    119-116    121-118  note shift in                                       2 proteins     4      50%    115-119    121-118     5      45%    115-119    120-117     6      30%    115-119    117      117 hydrophobic &                                       philic     7      35%    115-119    121-118     8      60%    115-119    117-121     9      50%    115-119    117-121     10     65%    115-119    117-121     11     50%    115-119    117      117 hydrophobic                                       & philic     12     55%    115-119    117      117 hydrophobic                                       & philic     ______________________________________

                  TABLE 13     ______________________________________     Number of Conserved Subgroups by Position Number in     Clusters of Subgroups with Different Minimum Pairwise Identities (MPI)     in Segments 19 and 20 of Protein Kinase     position             MPI     number  90      85    80     70  60     50  40%     ______________________________________     177     12      4     4      1   0      0   0     178     12      6     5      4   2      1   0     179     12      6     6      6   3      5   1     180     11      5     5      4   1      3   1     181     12      5     5      4   3      1   0     182     12      6     6      6   5      6   4     183     12      6     6      6   5      6   4     184     12      6     6      6   5      6   4     185     12      6     6      6   5      3   1     186     12      6     6      5   5      2   1     187     12      5     5      6   4      4   1     188     12      5     5      3   3      2   0     189     10      5     5      6   4      1   0     190      9      5     1      3   1      1   0     191     10      5     4      3   2      0   0     192     11      4     4      5   0      1   0     193     11      6     4      5   3      0   0     194      9      4     3      6   2      3   0     ______________________________________

                  TABLE 14     ______________________________________     Summary of Chemical Modification Data of Protein Kinases     Pertaining to Assignments to the Active Site     Position            Relevant Protection                          Conservation                                      Assigned Role     ______________________________________     K 007  MgATP + peptide                          not conserved                                      binds peptide     K 046  MgATP         conserved   gamma P     K 050  MgATP + peptide                          not conserved                                      binds peptide     E 067  MgATP         conserved   Mg ligand     E 089  not protected not conserved                                      no role     G 113  peptide       not conserved                                      binds peptide     M 116  peptide       not conserved                                      binds peptide     D 157  not labeled   conserved   Mg ligand     N 162  no information                          conserved   binds ribose     E 161  MgATP + peptide                          not conserved                                      AS vicinity     D 182  MgATP         conserved   general base     T 199  peptide       not conserved                                      binds peptide     C 201  peptide       not conserved                                      binds peptide     E 210  not labeled   conserved   structural     D 227  not labeled   almost conserved                                      structural     E 237  MgATP + peptide                          not conserved                                      AS no role     D 260  not protected not conserved                                      no role     R 305  no information                          conserved   uncertain     ______________________________________

                  TABLE 15     ______________________________________     Covariation at Positions 87 and 108     in Functional Subgroups 7 and 11 Protein Kinase                Funct Subgroup                7           11     ______________________________________                  ∘∘∘∘+++                                +++++++∘      87          LLPLRRR       RRRKKRRS     108          QQQQEEE       EEEEEEEP                  ∘∘∘∘---                                -------∘     ______________________________________      ∘ indicates an uncharged residue      + indicates a residue bearing a positive charge      - indicates a residue bearing a negative charge

                                      TABLE 16     __________________________________________________________________________     Small Alignment for Pathogenesis Related Proteins from Plants     __________________________________________________________________________         0   0    1    1    2    2    3    3    4    4    5    5    6    6     7   7         1   5    0    5    0    5    0    5    0    5    0    5    0    5     0   4       QNSPQDYLAVHNDARAQVGVGPMSWDANLASRAQNYANSRAGDCNLIHSGA  GENLAKGGGDF     TGRAAVQ       QNSPQDYLAVHNDARAQVGVGPMSWDANLASRAQNYANSRAGDCNLIHSGA  GENLAKGGGDF     TGRAAVQ       QNSPQDYLAVHNDARAQVGVGPMSWDANLASRAQNYANSRAGDCNLIHSGA  GENLAKGGGDF     TGRAAVQ       QNSQQDYLDAHNTARADVGVEPLTWDDQVAAYAQNYASQLAADCNLVHSHGQYGENLAWGSGDF     LTAAKAVE      AQNSQQDYLDAHNTARADVGVEPLTWDDQVAAYAQNYASQLAADCNLVHSHGQYGENLAEGSGDF     MTAAKAVE      AQNSQQDYLDAHNTARADVGVEPLTWDNGVAAYAQNYVSQLAADCNLVHSHGQYGENLAQGSGDF     MTAAKAVE          PQETLVVHNKARAMVGVGPMVWNETLATYAQSYAHERARDCAMKHSLGPFGENLAAGWGT     MSGPVATE     h - SENSPQDYLTPQNSARAAVGVGPVTWSTKLQQFAEKYAAQRAGDCRLQHSGGPYGENIFWGSAGFDWKAV     DAVR         .....*..* ..* *** ***.*..*.  .. .*..*. ..*.**.. **....***.. *.........      *..         7    8    8    9    9    0    0    1    1    2    2    3    3    4         5    0    5    0    5    0    5    0    5    0    5    0    5    0      LWVSERPSYNYATNQCVGGKKCRHYTQVVWRNSVRLGCGRARCNNGWWF ISCNYDPVGNWLGQRPY      LWVSERPSYNYATNQCVGGKKCRHYTQVVWRNSVRLGCGRARCNNGWWF ISCNYDPVGNWIGQRPY      LWVSERPDYNYATNQCVGGKMCGHYTQVVWRNSVRLGCGRARCNNGWWF ISCNYDPVGNWVGERPY      MWVNEKQYYAHDSNTCAQGQVCGHYTQVVWRNSVRVGCARVQCNNGGYI VSCNYDPPGNVIGKSPY      MWVDEKQYYDHDSNTCAQGQVCGHYTQVVWRNSVRVGCARVQCNNGGYV VSCNYDPPGNYRGESPY      MWVDEKQYYDHDSNTCAQGQVCGHYTQVVWRNSVRVGCARVKCNNGGYV VSCNYDPPGNVIGQSPY      YWMTEKENYDYDSNTCGGDGVCGHYTQIVWRDSVRLGCASVRCKNDEYIWVICSYDPPGNYIGQRPY      SWVDEKQWYNYATNSCAAGKVCGHYTQVVWRATTSIGCARVVCRDNRGVFIICNYEPRGNIAGMKPY          *..*...*....*.*.....*.****.***.....**....*........ *.*.*.** .*..**     __________________________________________________________________________

                                      TABLE 17     __________________________________________________________________________     Large Alignment for Pathogenesis Related Proteins and their     __________________________________________________________________________     Homologues            2    3    3    4    4    5           5    6    6      7    7    8       8            5    0    5    0    5    0           5    0    5      0    5    0       5     -MSWDANLASRAQNYANSRAGDCNLIHSGA         GENLAKGGGDF   TG RAAVQLWVSERPSYNYA     -LTWDDQVAAYAQNYASQLAADCNLVHSHG       QYGENLAEGSGDF  MTA AKAVEMWVDEKQYYDHD     N     -LTWDNGVAAYAQNYVSQLAADCNLVHSHG       QYGENLAQGSGDF  MTA AKAVEMWVDEKQYYDHD     O     -LTWDDQVAAYAQNYASQLAADCNLVHSHG       QYGENLAWGSGDF  LTA AKAVEMWVNEKQYYAHD     N     -MTWDNRLAAYAQNYANQRIGDCGMIHSHG       PYGENLAAA   FPQLNA AGAVKMWVDEKRFYDYN     O      LRVEWDHDAYVNAQKWANR    CIYNHSPLQHRTTTLKCGENLFMANYP    ASWSSVIQDWYDESLDFVF     G      LKMEWSIQATTNAQKWANK    CILEHSSKDDRKINIRCGENLYMSTDP    TLWSTVIQSWYNENEDFVY     G      LKMEWSREVTTNAQRWANK    CTLQHSDPEDRKTSTRCGENLYMSSDP    TSWSSAIQSWYDEILDFVY     G      NVLVWNDELAKIAQTWANQ    CDFNHDDCRN TAKYQVGQNIAISSTTATQFDRPSKLIKQWEDEVTEFNY     K      NVLVWNDELAKIAQTWANQ    CSFGHDQCRN TEKYQVGQNVAIASTTGNSYATMSKLIEMWEMEVKDFNP     K           . *.....  ** ........* .#*.    ..   . *.*..  .    . .  .  ...* .*     ....                        1    1    1    1     1     1    1    1    1            9    9      0    0    1    1     2     2    3    3    4            0    5      0    5    0    5     0     5    0    5    0      TNQCVGGKKC  RHYTQVVWRNSVRLGCGRARC NNGW  WFISCNYDPVGNWIGQR--      SNTCAQGQVC  GHYTQVVWRNSVRVGCARVQC NNGG  YVVSCNYDPPGNYRGES--      SNTCAQGQVC  GHYTQVVWRNSVRVGCARVKC NNGG  YVVSCNYDPPGNVIGQS--      SNTCAQGQVC  GHYTQVVWRNSVRVGCARVQC NNGG  YIVSCNYDPPGNVIGKS--      SNSCV GGVC  GHYTQVVWRNSVRLGCARVRS NNGW  FFITCNYDPPGNFIGQR--      FGPKKVG  VKVGHYTQVVWNSTFLVACGVAECPDQ PLKYFYVCHYCPGGNYVGRLYSPYTEGEPCDSCPGN     C      VGAK PN  SAVGHYTQLVWYSSFKIGCGIAYCPNQDNLKYFYVCHYCPMGNNVMKKSTPYQQGTPCASCPNN     C      VGPKSPN  AVVGHYTQLVWYSTYQVGCGIAYCPNQDSLKYYYVCQYCPAGNNMNRKNTPYQQGTPCAGCPDD     C      VG LQNSNFRKVGHYTQMVWGKTKEIGCGSIKYIEDNWYTHYLVCNYGPGGNDFNQ----      KGTMGDNNFSKVGHYTQMVWGKTKEIGCGSVKYIENNWHTHYLVCNYGPAGNYMDQ----          ..   .   ...**** **  .   .*. . . .. .  .. .*.* *.**. ...  ..  . ..     .. ..     __________________________________________________________________________

                  TABLE 18     ______________________________________     Positional Assignments for the Pathogenesis Related Proteins     without their Homologues     ______________________________________     001   PARSE; gap in proteins a,d,c,g, and b.     002   PARSE; gap in protein b; otherwise, polar split (Q/E); surface     003   PARSE; gap in protein b; otherwise, APC N; no assignment     004   PARSE; gap in protein b; otherwise, APC S; no assignment     005   Neutral split (PQPP); parse     006   APC Q; active site     007   Polar split (DDED), surface     008   Hydrophobic split (YYTY), inside     009   APCL; INSIDE     010   Amphiphilic split (ADVT); no assignment     011   Hydrophobic split (VAVP); inside     012   Neutral split; no assignment     013   APC N; active site     014   Polar split (DTKS); surface     015   APC A; inside     016   APC R; active site     017   APC A; inside     018   Amphiphilic split (QDMA); no assignment     019   APC V; INSIDE     020   APC G; parse, inside     021   APC V; INSIDE     022   Hydrophilic split (GEGG); surface     023   APC P; PARSE     024   Hydrophobic split (MLMV); inside     025   Hydrophobic split (STVT); inside     026   APC W; INSIDE     027   Polar split (DDNS); surface     028   Polar variable, one subgroup; surface     029   Polar variable, one subgroup; surface     030   Hydrophobic split (LVLL); inside     031   Neutral split (AAAQ); no assignment     032   Neutral split (SATQ); no assignment     033   Amphiphilic split (RYYF); no assignment     034   APC A; inside     035   Polar split (QQQE); surface     036   Polar split (NNSK); surface     037   APC Y; INSIDE     038   Hydrophobic single variable; inside     039   Polar split (NSHA); surface     040   Polar split (SQEQ); surface     041   Amphiphilic split (RLRR); no assignment     042   APC A; inside     043   Polar split (GARG); surface     044   APC D; active site     045   APC C; disulfide bridge     046   Polar split (NNAR); surface     047   Hydrophobic split (LLML); inside     048   Amphiphilic split (IVKQ); no assignment     049   APC H; active site     050   APC S; active site     051   Hydrophobic split (GHLG); inside     052   Neutral split (AGGG); no assignment     053   PARSE; gap in proteins a, d, and c. Confirmed by P b and h.     054   PARSE; gap in proteins a, d, and c. Otherwise inside.     055   APC G; parse, inside     056   APC E; active site     057   APC N; active site     058   Hydrophobic split (LLLI); inside     059   Hydrophobic split (AAAF); inside     060   Polar variable, one subgroup; surface     061   APC G; parse, inside     062   Hydrophobic split (GSWS); inside     063   Neutral split (GGGA); no assignment     064   Polar split (DDTG); surface     065   PARSE; gap in protein b; otherwise inside     066   PARSE; gap     067   PARSE; gap in proteins a, c, and d; otherwise inside     068   Polar split (TTSK); surface     069   Neutral split (GAGA); no assignment     070   Amphiphilic split (RAPV); no assignment; weak parse     071   Amphiphilic split (AKVD); no assignment     072   APC A; inside     073   Hydrophobic split (VVTV); inside     074   Polar split (QEER); surface     075   Hydrophobic split (LMYS); inside     076   APC W; INSIDE     077   Hydrophobic split (VVMV); inside     078   Polar variable, one subgroup; surface     079   APC E; active site     080   Polar split (RKKK); surface     081   Polar split (PQEQ); surface; weak parse     082   Amphiphilic variable, one subgroup; surface     083   APC Y; inside     084   Polar variable, one subgroup; surface     085   Hydrophobic split (YHYY); inside     086   Polar split (ADDA); surface     087   Neutral split (TSST); no assignment     088   APC E; active site     089   Polar split (QTTS); surface     090   APC C; disulfide bridge     091   Hydrophobic split (VAGA); inside     092   Neutral split (GQGA); no assignment; parse     093   Polar split (GGDG); surface; parse     094   Polar split (KQGK); surface     095   Polar variable, one subgroup; surface     096   APC C; disulfide bridge     097   Polar variable, one subgroup; surface     098   APC H; active site     099   APC Y; inside     100   APC T; inside     101   APC Q; active site     102   Hydrophobic split (VVIV); inside     103   APC V; inside     104   APC W; inside     105   APC R; active site     106   Polar split (NNDA); surface     107   Neutral split (SSST); no assignment     108   Hydrophobic split (VVVT); inside     109   Polar split (RRRS); surface     110   Hydrophobic split (LVLI); inside     111   APC G; parse     112   APC C; disulfide bridge     113   Neutral split (GAAA); no assignment     114   Polar split (RRSR); surface     115   Hydrophobic split (AVVV); inside     116   Amphiphilic variable, one subgroup; surface     117   APC C; disulfide bridge     118   Polar split (NNKR); surface     119   Polar split (NNND); surface     120   Polar split (GGDN); surface     121   Amphiphilic split (WGER); no assignment     122   Hydrophobic split (WYYG); inside     123   Hydrophobic variable, one subgroup; inside     124   PARSE; gap in protein a, d, c, g, e, and f; otherwise inside     125   Hydrophobic split (IVVI); inside     126   Hydrophobic split (SSII); inside     127   APC C; disulfide bridge     128   Polar split (NNSN); surface     129   APC Y; INSIDE     130   Polar split (DDDE); surface     131   APC P; PARSE     132   Amphiphilic split (VPPR); no assignment; parse     133   APC G; parse; inside     134   APC N; active site     135   Hydrophobic variable, one subgroup; inside     136   SURFACE; CM1 Hb; inside     137   APC G; parse     138   SURFACE     139   Polar split (RSRK); surface     140   APC P; PARSE     141   APC Y; INSIDE     ______________________________________

                  TABLE 19a     ______________________________________     Revised Positional Assignments for Segment 2a of the     Pathogenesis Related Proteins and their Homologues     ______________________________________     23        Parse     24        Hydrophobic variable; inside     25        Amphiphilic single variable; no assignment     26        APC W; inside     27        Polar single variable     28        Surface, 2 variable subgroups     29        Surface, 2 variable subgroups     30        Hydrophobic variable; inside     31        Hydrophobic single variable; inside     32        Surface assignment, criterion ii     33        Amphiphilic single variable; no assignment     34        APC A. inside     35        CM1 Q (note maize sequence)     36        Polar single variable     37        Hydrophobic split (YWW); inside     38        Hydrophobic single variable; inside     39        Polar single variable     40        Surface, 2 variable subgroups     41        Parse     ______________________________________

                  TABLE 19b     ______________________________________     Revised Positional Assignments for Segment 2b of the     Pathogenesis Related Proteins and their Homologues     ______________________________________     45        APC C     46        Surface, 3 variable subgroups     47        Hydrophobic variable, 2 subgroups; inside     48        Surface, 2 variable subgroups     49        APC H     50        Polar split (SSD); no longer APC     51        Surface, 3 variable subgroups     52        Surface, 2 variable subgroups     53        Parse     ______________________________________

                  TABLE 19c     ______________________________________     Revised Positional Assignments for Segment 3 of the     Pathogenesis Related Proteins and their Homologues     ______________________________________     55  APC G; parse     56  Polar split (EEQ); no longer APC E     57  APC N     58  Hydrophobic single variable; inside     59  Hydrophobic single variable; inside     60  Amphiphilic single variable; surface in pathogenesis related         proteins;         inside in venom allergen and TPX     61  Hydrogen bonding variable; no longer APC G     62  Parse, but deletion could be rearranged; surface     63  Parse, but deletion could be rearranged; surface     64  Parse     ______________________________________

                  TABLE 19d     ______________________________________     Revised Positional Assignments for Segments 4a and 4b of     the Pathogenesis Related Proteins and their Homologues     ______________________________________     067     Parse     068     Surface, 2 variable subgroups (deletion is moved to the left)     069     Hydrophobic variable, 2 subgroups; weak parse     070     Polar single variable     071     Surface, 2 variable subgroups     072     Hydrophobic single variable; inside     073     Hydrophobic split; inside     074     Surface, 2 variable subgroups     075     Surface, 2 variable subgroups     076     APC W; inside     077     Amphiphilic split (VYE)     078     Surface, 3 variable subgroups     079     APC E     080     Surface, 2 variable subgroups     081     Surface, 3 variable subgroups     082     Surface, 2 variable subgroups     083     Hydrophobic split (YFF) inside     084     Amphiphilic single variable     085     Hydrophobic variable, 3 subgroups; inside.     086     Polar single variable     087     Surface, 2 variable subgroups     088     Polar split (NGG)     089     Parse.     ______________________________________

                  TABLE 19e     ______________________________________     Revised Positional Assignments for Segments 4c and 4d of the     Pathogenesis Related Proteins and their Homologues     ______________________________________      95       Surface; 2 variable subgroups      96       Hydrophobic split (CVV); inside      97       Polar split (RGG)      98       APC H; active site      99       APC Y; inside     100       APC T; inside     101       APC Q; active site     102       Hydrophobic single variable; inside     103       APC V; inside     104       APC W; inside     105       No longer APC R; polar single variable     106       Polar split (NSK)     107       Hydrogen bonding single variable     108       Amphiphilic variable     109       Polar single variable     110       Hydrophobic variable, two subgroups; inside     111       CM1 G; inside, parse     112       APC C     113       Neutral single variable     114       Amphiphilic single variable     115       Hydrophobic variable; inside     116       Surface, 2 variable subgroups     117       No longer APC C; hydrophobic single variable     117'      Parse     118       Polar single variable     119       Polar single variable     120       Parse     ______________________________________

                  TABLE 19f     ______________________________________     Revised Positional Assignments for Segment 5 of the     Pathogenesis Related Proteins and their Homologues     ______________________________________     122       Parse     123       Hydrophobic single variable; inside     124       Hydrophobic variable, 2 subgroups; inside     125       Hydrophobic single variable; inside     126       Hydrophobic single variable; inside     127       APC C     128       Polar single variable     129       APC Y; inside     130       Polar split (DCG)     131       Parse; APC P     ______________________________________

                  TABLE 19g     ______________________________________     Revised Positional Assignments for Segment 6 of the     Pathogenesis Related Proteins and their Homologues     ______________________________________     131      Parse; APC P     132      Parse     133      APC G; parse     134      APC N     135      Surface, 3 variable subgroups     136      Surface, 3 variable subgroups; Also CM1 Hb; inside     137      Surface, 2 variable subgroups     138      Surface, 2 variable subgroups     139      Parse; surface, 2 variable subgroups     140      Parse     ______________________________________

                  TABLE 20     ______________________________________     Predicted Secondary Structure for     Pathogenesis Related Proteins and their Homologues     Positions  Secondary Structure Prediction                                   Length     ______________________________________     1-4        Coil                4      5-20      Helix              16     21-22      Coil                2     23-41      Helix              19     42-44      Coil                3     45-50      Beta                6     51-54      Coil                4     55-60      Beta (possibly extend 54-61)                                    6     61-67      Coil                7     68-86      Helix (possibly bent at position 81)                                   19     87-97      Coil (tied by disulfide loop)                                   11      98        Active site         1      99-117    Helix              19     118-122    Coil                5     123-130    Beta                8     131        Parse               1     132-136    Beta                4     137-140    Coil                4     ______________________________________

    __________________________________________________________________________     #             SEQUENCE LISTING     - (1) GENERAL INFORMATION:     -    (iii) NUMBER OF SEQUENCES: 114     - (2) INFORMATION FOR SEQ ID NO:1:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 260     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: bovine al - #pha     #Column 1) FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:     - Asp Gln Phe Glu Arg Ile Lys Thr Leu Gly Th - #r Gly Ser Phe Gly     #               015     - Arg Val Met Leu Val Lys His Met Glu Thr Gl - #y Asn His Tyr Ala     #               030     - Met Lys Ile Leu Asp Lys Gln Lys Val Val Ly - #s Leu Lys Gln Ile     #               045     - Glu His Thr Leu Asn Glu Lys Arg Ile Leu Gl - #n Ala Val Asn Phe     #               060     - Pro Phe Leu Val Lys Leu Glu Phe Ser Phe Ly - #s Asp Asn Ser Asn     #               075     - Leu Tyr Met Val Met Glu Tyr Val Pro Gly Gl - #y Glu Met Phe Ser     #               090     - His Leu Arg Arg Ile Gly Arg Phe Ser Glu Pr - #o His Ala Arg Phe     #               105     - Tyr Ala Ala Gln Ile Val Leu Thr Phe Glu Ty - #r Leu His Ser Leu     #               120     - Asp Leu Ile Tyr Arg Asp Leu Lys Pro Glu As - #n Leu Leu Ile Asp     #               135     - Gln Gln Gly Tyr Ile Gln Val Thr Asp Phe Gl - #y Phe Ala Lys Arg     #               150     - Val Lys Gly Arg Thr Trp Thr Leu Cys Gly Th - #r Pro Glu Tyr Leu     #               165     - Ala Pro Glu Ile Ile Leu Ser Lys Gly Tyr As - #n Lys Ala Val Asp     #               180     - Trp Trp Ala Leu Gly Val Leu Ile Tyr Glu Me - #t Ala Ala Gly Tyr     #               195     - Pro Pro Phe Phe Ala Asp Gln Pro Ile Gln Il - #e Tyr Glu Lys Ile     #               210     - Val Ser Gly Lys Val Arg Phe Pro Ser His Ph - #e Ser Ser Asp Leu     #               225     - Lys Asp Leu Leu Arg Asn Leu Leu Gln Val As - #p Leu Thr Lys Arg     #               240     - Phe Gly Asn Leu Lys Asp Gly Val Asn Asp Il - #e Lys Asn His Lys     #               255     - Trp Phe Ala Thr Thr                     260     - (2) INFORMATION FOR SEQ ID NO:2:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 260     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: mouse alp - #ha     #sequence, Table 8 Column 2 kinase; literature     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:     - Asp Gln Phe Asp Arg Ile Lys Thr Leu Gly Th - #r Gly Ser Phe Gly     #               015     - Arg Val Met Leu Val Lys His Lys Glu Ser Gl - #y Asn His Tyr Ala     #               030     - Met Lys Ile Leu Asp Lys Gln Lys Val Val Ly - #s Leu Lys Gln Ile     #               045     - Glu His Thr Leu Asn Glu Lys Arg Ile Leu Gl - #n Ala Val Asn Phe     #               060     - Pro Phe Leu Val Lys Leu Glu Phe Ser Phe Ly - #s Asp Asn Ser Asn     #               075     - Leu Tyr Met Val Met Glu Tyr Val Ala Gly Gl - #y Glu Met Phe Ser     #               090     - His Leu Arg Arg Ile Gly Arg Phe Ser Glu Pr - #o His Ala Arg Phe     #               105     - Tyr Ala Ala Gln Ile Val Leu Thr Phe Glu Ty - #r Leu His Ser Leu     #               120     - Asp Leu Ile Tyr Arg Asp Leu Lys Pro Glu As - #n Leu Leu Ile Asp     #               135     - Gln Gln Gly Tyr Ile Gln Val Thr Asp Phe Gl - #y Phe Ala Lys Arg     #               150     - Val Lys Gly Arg Thr Trp Thr Leu Cys Gly Th - #r Pro Glu Tyr Leu     #               165     - Ala Pro Glu Ile Ile Leu Ser Lys Gly Tyr As - #n Lys Ala Val Asp     #               180     - Trp Trp Ala Leu Gly Val Leu Ile Tyr Glu Me - #t Ala Ala Gly Tyr     #               195     - Pro Pro Phe Phe Ala Asp Gln Pro Ile Gln Il - #e Tyr Glu Lys Ile     #               210     - Val Ser Gly Lys Val Arg Phe Pro Ser His Ph - #e Ser Ser Asp Leu     #               225     - Lys Asp Leu Leu Arg Asn Leu Leu Gln Val As - #p Leu Thr Lys Arg     #               240     - Phe Gly Asn Leu Lys Asp Gly Val Asn Asp Il - #e Lys Asn His Lys     #               255     - Trp Phe Ala Thr Thr                     260     - (2) INFORMATION FOR SEQ ID NO:3:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 260     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: bovine be - #ta     #Column 3) FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:     - Gly Asp Phe Glu Arg Lys Lys Thr Leu Gly Th - #r Gly Ser Phe Gly     #               015     - Arg Val Met Leu Val Lys His Lys Ala Thr Gl - #u Gln Tyr Tyr Ala     #               030     - Met Lys Ile Leu Asp Lys Gln Lys Val Val Ly - #s Leu Lys Gln Ile     #               045     - Glu His Thr Leu Asn Glu Lys Arg Ile Leu Gl - #n Ala Val Asn Phe     #               060     - Pro Phe Leu Val Arg Leu Glu Tyr Ala Phe Ly - #s Asp Asn Ser Asn     #               075     - Leu Tyr Met Val Met Glu Tyr Val Pro Gly Gl - #y Glu Met Phe Ser     #               090     - His Leu Arg Arg Ile Gly Arg Phe Ser Glu Pr - #o His Ala Arg Phe     #               105     - Tyr Ala Ala Gln Ile Val Leu Thr Phe Glu Ty - #r Leu His Ser Leu     #               120     - Asp Leu Ile Tyr Arg Asp Leu Lys Pro Glu As - #n Leu Leu Ile Asp     #               135     - His Gln Gly Tyr Ile Gln Val Thr Asp Phe Gl - #y Phe Ala Lys Arg     #               150     - Val Lys Gly Arg Thr Trp Thr Leu Cys Gly Th - #r Pro Glu Tyr Leu     #               165     - Ala Pro Glu Ile Ile Leu Ser Lys Gly Tyr As - #n Lys Ala Val Asp     #               180     - Trp Trp Ala Leu Gly Val Leu Ile Tyr Glu Me - #t Ala Ala Gly Tyr     #               195     - Pro Pro Phe Phe Ala Asp Gln Pro Ile Gln Il - #e Tyr Glu Lys Ile     #               210     - Val Ser Gly Lys Val Arg Phe Pro Ser His Ph - #e Ser Ser Asp Leu     #               225     - Lys Asp Leu Leu Arg Asn Leu Leu Gln Val As - #p Leu Thr Lys Arg     #               240     - Phe Gly Asn Leu Lys Asn Gly Val Ser Asp Il - #e Lys Thr His Lys     #               255     - Trp Phe Ala Thr Thr                     260     - (2) INFORMATION FOR SEQ ID NO:4:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 260     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: mouse bet - #a     #Column 4) FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:     - Glu Asp Phe Glu Arg Lys Lys Thr Leu Gly Th - #r Gly Ser Phe Gly     #               015     - Arg Val Met Leu Val Lys His Lys Ala Thr Gl - #u Gln Tyr Tyr Ala     #               030     - Met Lys Ile Leu Asp Lys Gln Lys Val Val Ly - #s Leu Lys Gln Ile     #               045     - Glu His Thr Leu Asn Glu Lys Arg Ile Leu Gl - #n Ala Val Glu Phe     #               060     - Pro Phe Leu Val Arg Leu Glu Tyr Ser Phe Ly - #s Asp Asn Ser Asn     #               075     - Leu Tyr Met Val Met Glu Tyr Val Pro Gly Gl - #y Glu Met Phe Ser     #               090     - His Leu Arg Arg Ile Gly Arg Phe Ser Glu Pr - #o His Ala Arg Phe     #               105     - Tyr Ala Ala Gln Ile Val Leu Thr Phe Glu Ty - #r Leu His Ser Leu     #               120     - Asp Leu Ile Tyr Arg Asp Leu Lys Pro Glu As - #n Leu Leu Ile Asp     #               135     - His Gln Gly Tyr Ile Gln Val Thr Asp Phe Gl - #y Phe Ala Lys Arg     #               150     - Val Lys Gly Arg Thr Trp Thr Leu Cys Gly Th - #r Pro Glu Tyr Leu     #               165     - Ala Pro Glu Ile Ile Leu Ser Lys Gly Tyr As - #n Lys Ala Val Asp     #               180     - Trp Trp Ala Leu Gly Val Leu Ile Tyr Glu Me - #t Ala Ala Gly Tyr     #               195     - Pro Pro Phe Phe Ala Asp Gln Pro Ile Gln Il - #e Tyr Glu Lys Ile     #               210     - Val Ser Gly Lys Val Arg Phe Pro Ser His Ph - #e Ser Ser Asp Leu     #               225     - Lys Asp Leu Leu Arg Asn Leu Leu Gln Val As - #p Leu Thr Lys Arg     #               240     - Phe Gly Asn Leu Lys Asn Gly Val Ser Asp Il - #e Lys Thr His Lys     #               255     - Trp Phe Ala Thr Thr                     260     - (2) INFORMATION FOR SEQ ID NO:5:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 258     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Saccharomyce - #s cerevisiae     #Column 5) FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:     - Lys Asn Phe Gln Ile Leu Arg Thr Leu Gly Th - #r Gly Ser Phe Gly     #               015     - Arg Val His Leu Ile Arg Ser Arg His Asn Gl - #y Arg Tyr Tyr Ala     #               030     - Met Lys Val Leu Lys Lys Glu Ile Val Val Ar - #g Leu Lys Gln Val     #               045     - Glu His Thr Asn Asp Glu Arg Leu Met Leu Se - #r Ile Val Thr His     #               060     - Pro Phe Ile Ile Arg Met Trp Gly Thr Phe Gl - #n Asp Ala Gln Gln     #               075     - Ile Phe Met Ile Ile Leu Lys Val Gly Arg Le - #u Phe Ser Leu Leu     #               090     - Arg Lys Ser Lys Asp Phe Pro Asn Pro Val Al - #a Gln Ile Phe Ala     #               105     - Ala Glu Val Cys Leu Ala Leu Glu Tyr Leu Hi - #s Ser Lys Asp Ile     #               120     - Ile Tyr Arg Asp Leu Lys Pro Glu Asn Ile Le - #u Leu Asp Lys Asn     #               135     - Gly His Ile Lys Ile Thr Asp Phe Gly Phe Al - #a Lys Tyr Val Pro     #               150     - Asp Val Thr Tyr Thr Leu Cys Gly Thr Pro As - #p Tyr Ile Ala Pro     #               165     - Glu Val Val Ser Thr Lys Pro Tyr Asn Lys Se - #r Ile Asp Trp Trp     #               180     - Ser Phe Gly Ile Leu Ile Tyr Glu Met Leu Al - #a Gly Tyr Thr Pro     #               195     - Phe Tyr Asp Ser Asn Thr Met Lys Thr Tyr Gl - #u Lys Ile Leu Asn     #               210     - Ala Glu Leu Arg Phe Pro Pro Phe Phe Asn Gl - #u Asp Val Lys Asp     #               225     - Leu Leu Ser Arg Leu Ile Thr Arg Asp Leu Se - #r Gln Arg Leu Gly     #               240     - Asn Leu Gln Asn Gly Thr Glu Asp Val Lys As - #n His Pro Trp Phe     #               255     - Lys Glu Val     - (2) INFORMATION FOR SEQ ID NO:6:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 260     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Saccharomyce - #s cerevisiae     #Column 6) FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:     - Gln Asp Phe Gln Ile Leu Arg Thr Leu Gly Th - #r Gly Ser Phe Gly     #               015     - Arg Val His Leu Ile Arg Ser Arg His Asn Gl - #y Arg Tyr Tyr Ala     #               030     - Met Lys Val Leu Lys Lys Glu Ile Val Val Ar - #g Leu Lys Gln Val     #               045     - Glu His Thr Asn Asp Glu Arg Leu Met Leu Se - #r Ile Val Thr His     #               060     - Pro Phe Ile Ile Arg Met Trp Gly Thr Phe Gl - #n Asp Ala Gln Gln     #               075     - Ile Phe Met Ile Met Asp Tyr Ile Glu Gly Gl - #y Glu Leu Phe Ser     #               090     - Leu Leu Arg Lys Ser Gln Arg Phe Pro Asn Pr - #o Val Ala Lys Phe     #               105     - Tyr Ala Ala Glu Val Cys Leu Ala Leu Glu Ty - #r Leu His Ser Lys     #               120     - Asp Ile Ile Tyr Arg Asp Leu Lys Pro Glu As - #n Ile Leu Leu Asp     #               135     - Lys Asn Gly His Ile Lys Ile Thr Asp Phe Gl - #y Phe Ala Lys Tyr     #               150     - Val Pro Asp Val Thr Tyr Thr Leu Cys Gly Th - #r Pro Asp Tyr Ile     #               165     - Ala Pro Glu Val Val Ser Thr Lys Pro Tyr As - #n Lys Ser Ile Asp     #               180     - Trp Trp Ser Phe Gly Ile Leu Ile Tyr Glu Me - #t Leu Ala Gly Tyr     #               195     - Thr Pro Phe Tyr Asp Ser Asn Thr Met Lys Th - #r Tyr Glu Lys Ile     #               210     - Leu Asn Ala Glu Leu Arg Phe Pro Pro Phe Ph - #e Asn Glu Asp Val     #               225     - Lys Asp Leu Leu Ser Arg Leu Ile Thr Arg As - #p Leu Ser Gln Arg     #               240     - Leu Gly Asn Leu Gln Asn Gly Thr Glu Asp Va - #l Lys Asn His Pro     #               255     - Trp Phe Lys Glu Val                     260     - (2) INFORMATION FOR SEQ ID NO:7:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 260     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Saccharomyce - #s cerevisiae     #Column 7) FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7:     - His Asp Phe Gln Ile Met Arg Thr Leu Gly Th - #r Gly Ser Phe Gly     #               015     - Arg Val His Leu Val Arg Ser Val His Asn Gl - #y Arg Tyr Tyr Ala     #               030     - Ile Lys Val Leu Lys Lys Gln Gln Val Val Ly - #s Met Lys Gln Val     #               045     - Glu His Thr Asn Asp Glu Arg Arg Met Leu Ly - #s Leu Val Glu His     #               060     - Pro Phe Leu Ile Arg Met Trp Gly Thr Phe Gl - #n Asp Ala Arg Asn     #               075     - Ile Phe Met Val Met Asp Tyr Ile Glu Gly Gl - #y Glu Leu Phe Ser     #               090     - Leu Leu Arg Lys Ser Gln Arg Phe Pro Asn Pr - #o Val Ala Lys Phe     #               105     - Tyr Ala Ala Glu Val Ile Leu Ala Leu Glu Ty - #r Leu His Ala His     #               120     - Asn Ile Ile Tyr Arg Asp Leu Lys Pro Glu As - #n Ile Leu Leu Asp     #               135     - Arg Asn Gly His Ile Lys Ile Thr Asp Phe Gl - #y Phe Ala Lys Glu     #               150     - Val Gln Thr Val Thr Trp Thr Leu Cys Gly Th - #r Pro Asp Tyr Ile     #               165     - Ala Pro Glu Val Ile Thr Thr Lys Pro Tyr As - #n Lys Ser Val Asp     #               180     - Trp Trp Ser Leu Gly Val Leu Ile Tyr Glu Me - #t Leu Ala Gly Tyr     #               195     - Thr Pro Phe Tyr Asp Thr Thr Pro Met Lys Th - #r Tyr Glu Lys Ile     #               210     - Leu Gln Gly Lys Val Val Tyr Pro Pro Tyr Ph - #e Gln Pro Asp Val     #               225     - Val Asp Leu Leu Ser Lys Leu Ile Thr Ala As - #p Leu Thr Arg Arg     #               240     - Ile Gly Asn Leu Gln Asp Gly Ser Arg Asp Il - #e Lys Ala His Pro     #               255     - Trp Phe Ser Glu Val                     260     - (2) INFORMATION FOR SEQ ID NO:8:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 260     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Saccharomyce - #s cerevisiae     #Column 8) FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8:     - Ser Asp Phe Gln Ile Leu Arg Thr Leu Gly Th - #r Gly Ser Phe Gly     #               015     - Arg Val His Leu Ile Arg Ser Asn His Asn Gl - #y Arg Phe Tyr Ala     #               030     - Leu Lys Thr Leu Lys Lys His Thr Ile Val Ly - #s Leu Lys Gln Val     #               045     - Glu His Thr Asn Asp Glu Arg Arg Met Leu Se - #r Ile Val Ser His     #               060     - Pro Phe Ile Ile Arg Met Trp Gly Thr Phe Gl - #n Asp Ser Gln Gln     #               075     - Val Phe Met Val Met Asp Tyr Ile Glu Gly Gl - #y Glu Leu Phe Ser     #               090     - Leu Leu Arg Lys Ser Gln Arg Phe Pro Asn Pr - #o Val Ala Lys Phe     #               105     - Tyr Ala Ala Glu Val Cys Leu Ala Leu Glu Ty - #r Leu His Ser Lys     #               120     - Asp Ile Thr Tyr Arg Asp Leu Lys Pro Glu As - #n Ile Leu Leu Asp     #               135     - Lys Asn Gly His Ile Lys Ile Thr Asp Phe Gl - #y Phe Ala Lys Tyr     #               150     - Val Pro Asp Val Thr Tyr Thr Leu Cys Gly Th - #r Pro Asp Tyr Ile     #               165     - Ala Pro Glu Val Val Ser Thr Lys Pro Tyr As - #n Lys Ser Val Asp     #               180     - Trp Trp Ser Phe Gly Val Leu Ile Tyr Glu Me - #t Leu Ala Gly Tyr     #               195     - Thr Pro Phe Tyr Asn Ser Asn Thr Met Lys Th - #r Tyr Glu Asn Ile     #               210     - Leu Asn Ala Glu Leu Lys Phe Pro Pro Phe Ph - #e His Pro Asp Ala     #               225     - Gln Asp Leu Leu Lys Lys Leu Ile Thr Arg As - #p Leu Ser Glu Arg     #               240     - Leu Gly Asn Leu Gln Asn Gly Ser Glu Asp Va - #l Lys Asn His Pro     #               255     - Trp Phe Asn Glu Val                     260     - (2) INFORMATION FOR SEQ ID NO:9:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 264     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: bovine     #Column 9) FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9:     - Ser Asp Phe Asn Ile Ile Asp Thr Leu Gly Va - #l Gly Gly Phe Gly     #               015     - Arg Val Glu Leu Val Gln Leu Lys Ser Glu Gl - #u Ser Lys Thr Phe     #               030     - Ala Met Lys Ile Leu Lys Lys Arg His Ile Va - #l Asp Thr Arg Gln     #               045     - Gln Glu His Ile Arg Ser Glu Lys Gln Ile Me - #t Gln Gly Ala His     #               060     - Ser Asp Phe Ile Val Arg Leu Tyr Arg Thr Ph - #e Lys Asp Ser Lys     #               075     - Tyr Leu Tyr Met Leu Met Glu Ala Cys Leu Gl - #y Gly Glu Leu Trp     #               090     - Thr Ile Leu Arg Asp Arg Gly Ser Phe Glu As - #p Ser Thr Thr Arg     #               105     - Phe Tyr Thr Ala Cys Val Val Glu Ala Phe Al - #a Tyr Leu His Ser     #               120     - Lys Gly Ile Ile Tyr Arg Asp Leu Lys Pro Gl - #u Asn Leu Ile Leu     #               135     - Asp His Arg Gly Tyr Ala Lys Leu Val Asp Ph - #e Gly Phe Ala Lys     #               150     - Lys Ile Gly Phe Gly Lys Leu Thr Trp Thr Ph - #e Cys Gly Thr Pro     #               165     - Glu Tyr Val Ala Pro Glu Ile Ile Leu Asn Ly - #s Gly His Asp Ile     #               180     - Ser Ala Asp Tyr Trp Ser Leu Gly Ile Leu Me - #t Tyr Glu Leu Leu     #               195     - Thr Gly Ser Pro Pro Phe Ser Gly Pro Asp Pr - #o Met Lys Thr Tyr     #               210     - Asn Ile Ile Leu Arg Gly Ile Asp Ile Glu Ph - #e Pro Lys Lys Ile     #               225     - Ala Lys Asn Ala Ala Asn Leu Ile Lys Lys Le - #u Cys Arg Asp Asn     #               240     - Pro Ser Glu Arg Leu Gly Asn Leu Lys Asn Gl - #y Val Lys Asp Ile     #               255     - Gln Lys His Lys Trp Phe Glu Gly Phe                     260     - (2) INFORMATION FOR SEQ ID NO:10:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 264     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: bovine     #Column 11 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10:     - Thr Asp Phe Asn Phe Leu Met Val Leu Gly Ly - #s Gly Ser Phe Gly     #               015     - Lys Val Met Leu Ala Asp Arg Lys Gly Thr Gl - #u Glu Leu Tyr Ala     #               030     - Ile Lys Ile Leu Lys Lys Asp Val Val Ile Gl - #n Asp Asp Asp Val     #               045     - Glu Cys Thr Met Val Glu Lys Arg Val Leu Al - #a Leu Leu Asp Lys     #               060     - Pro Pro Phe Leu Thr Gln Leu His Ser Cys Ph - #e Gln Thr Val Asp     #               075     - Arg Leu Tyr Phe Val Met Glu Tyr Val Asn Gl - #y Gly Asp Leu Met     #               090     - Tyr His Ile Gln Gln Val Gly Lys Phe Lys Gl - #u Pro Gln Ala Val     #               105     - Phe Tyr Ala Ala Glu Ile Ser Ile Gly Leu Ph - #e Phe Leu His Lys     #               120     - Arg Gly Ile Ile Tyr Arg Asp Leu Lys Leu As - #p Asn Val Met Leu     #               135     - Asp Ser Glu Gly His Ile Lys Ile Ala Asp Ph - #e Gly Met Cys Lys     #               150     - Glu His Met Met Asp Gly Val Thr Thr Arg Th - #r Phe Cys Gly Thr     #               165     - Pro Asp Tyr Ile Ala Pro Glu Ile Ile Ala Ty - #r Gln Pro Tyr Gly     #               180     - Lys Ser Val Asp Trp Trp Ala Tyr Gly Val Le - #u Leu Tyr Glu Met     #               195     - Leu Ala Gly Gln Pro Pro Phe Asp Gly Glu As - #p Glu Asp Glu Leu     #               210     - Phe Gln Ser Ile Met Glu His Asn Val Ser Ty - #r Pro Lys Ser Leu     #               225     - Ser Lys Glu Ala Val Ser Ile Cys Lys Gly Le - #u Met Thr Lys His     #               240     - Pro Gly Lys Arg Leu Gly Cys Gly Pro Glu Gl - #y Glu Arg Asp Val     #               255     - Arg Glu His Ala Phe Phe Arg Arg Ile                     260     - (2) INFORMATION FOR SEQ ID NO:11:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 264     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: rabbit     #Column 12 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11:     - Thr Asp Phe Asn Phe Leu Met Val Leu Gly Ly - #s Gly Ser Phe Gly     #               015     - Lys Val Met Leu Ser Glu Arg Lys Gly Thr As - #p Glu Leu Tyr Ala     #               030     - Val Lys Ile Leu Lys Lys Asp Val Val Ile Gl - #n Asp Asp Asp Val     #               045     - Glu Cys Thr Met Val Glu Lys Arg Val Leu Al - #a Leu Pro Gly Lys     #               060     - Pro Pro Phe Leu Thr Gln Leu His Ser Cys Ph - #e Gln Thr Met Asp     #               075     - Arg Leu Tyr Phe Val Met Glu Tyr Val Asn Gl - #y Gly Asp Leu Met     #               090     - Tyr His Ile Gln Gln Val Gly Arg Phe Lys Gl - #u Pro His Ala Val     #               105     - Phe Tyr Ala Ala Glu Ile Ala Ile Gly Leu Ph - #e Phe Leu Gln Ser     #               120     - Lys Gly Ile Ile Tyr Arg Asp Leu Lys Leu As - #p Asn Val Met Leu     #               135     - Asp Ser Glu Gly His Ile Lys Ile Ala Asp Ph - #e Gly Met Cys Lys     #               150     - Glu Asn Ile Trp Asp Gly Val Thr Thr Lys Th - #r Thr Cys Gly Thr     #               165     - Pro Asp Tyr Ile Ala Pro Glu Ile Ile Ala Ty - #r Gln Pro Tyr Gly     #               180     - Lys Ser Val Asp Trp Trp Ala Phe Gly Val Le - #u Leu Tyr Glu Met     #               195     - Leu Ala Gly Gln Ala Pro Phe Glu Gly Glu As - #p Glu Asp Glu Leu     #               210     - Phe Gln Ser Ile Met Glu His Asn Val Ala Ty - #r Pro Lys Ser Met     #               225     - Ser Lys Glu Ala Val Ala Ile Cys Lys Gly Le - #u Ile Thr Lys His     #               240     - Pro Gly Lys Arg Leu Gly Cys Gly Pro Glu Gl - #y Glu Arg Asp Ile     #               255     - Lys Glu His Ala Phe Phe Arg Tyr Ile                     260     - (2) INFORMATION FOR SEQ ID NO:12:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 264     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: bovine     #Column 13 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12:     - Thr Asp Phe Asn Phe Leu Met Val Leu Gly Ly - #s Gly Ser Phe Gly     #               015     - Lys Val Met Leu Ser Glu Arg Lys Gly Thr As - #p Glu Leu Tyr Ala     #               030     - Val Lys Ile Leu Lys Lys Asp Val Val Ile Gl - #n Asp Asp Asp Val     #               045     - Glu Cys Thr Met Val Glu Lys Arg Val Leu Al - #a Leu Pro Gly Lys     #               060     - Pro Pro Phe Leu Thr Gln Leu His Ser Cys Ph - #e Gln Thr Met Asp     #               075     - Arg Leu Tyr Phe Val Met Glu Tyr Val Asn Gl - #y Gly Asp Leu Met     #               090     - Tyr His Ile Gln Gln Val Gly Arg Phe Lys Gl - #u Pro His Ala Val     #               105     - Phe Tyr Ala Ala Glu Ile Ala Ile Gly Leu Ph - #e Phe Leu Gln Ser     #               120     - Lys Gly Ile Ile Tyr Arg Asp Leu Lys Leu As - #p Asn Val Met Leu     #               135     - Asp Ser Glu Gly His Ile Lys Ile Ala Asp Ph - #e Gly Met Cys Lys     #               150     - Glu Asn Ile Trp Asp Gly Val Thr Thr Lys Th - #r Phe Cys Gly Thr     #               165     - Pro Asp Tyr Ile Ala Pro Glu Ile Ile Ala Ty - #r Gln Pro Tyr Gly     #               180     - Lys Ser Val Asp Trp Trp Ala Phe Gly Val Le - #u Leu Tyr Glu Met     #               195     - Leu Ala Gly Gln Ala Pro Phe Glu Gly Glu As - #p Glu Asp Glu Leu     #               210     - Phe Gln Ser Ile Met Glu His Asn Val Ala Ty - #r Pro Lys Ser Met     #               225     - Ser Lys Glu Ala Val Ala Ile Cys Lys Gly Le - #u Met Thr Lys His     #               240     - Pro Gly Lys Arg Leu Gly Cys Gly Pro Glu Gl - #y Glu Arg Asp Ile     #               255     - Lys Asp His Ala Phe Phe Arg Tyr Ile                     260     - (2) INFORMATION FOR SEQ ID NO:13:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 263     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: rabbit     #Column 14 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:     - Asp Phe Asn Phe Leu Met Val Leu Gly Lys Gl - #y Ser Phe Gly Lys     #               015     - Val Met Leu Ser Glu Arg Lys Gly Thr Asp Gl - #u Leu Tyr Ala Val     #               030     - Lys Ile Leu Lys Lys Asp Val Val Ile Gln As - #p Asp Asp Val Glu     #               045     - Cys Thr Met Val Glu Lys Arg Val Leu Ala Le - #u Pro Gly Lys Pro     #               060     - Pro Phe Leu Thr Gln Leu His Ser Cys Phe Gl - #n Thr Met Asp Arg     #               075     - Leu Tyr Glu Val Met Glu Tyr Val Asn Gly Gl - #y Asp Leu Met Tyr     #               090     - His Ile Gln Gln Val Gly Arg Phe Lys Glu Pr - #o His Ala Val Phe     #               105     - Tyr Ala Ala Glu Ile Ala Ile Gly Leu Phe Ph - #e Leu Gln Ser Lys     #               120     - Gly Ile Ile Tyr Arg Asp Leu Lys Leu Asp As - #n Val Met Leu Asp     #               135     - Ser Glu Gly His Ile Lys Ile Ala Asp Phe Gl - #y Met Cys Lys Glu     #               150     - Asn Ile Trp Asp Gly Val Thr Thr Lys Thr Ph - #e Cys Gly Thr Pro     #               165     - Asp Tyr Ile Ala Pro Glu Ile Ile Ala Tyr Gl - #n Pro Tyr Asp Lys     #               180     - Ser Val Asp Trp Trp Ser Phe Gly Val Leu Le - #u Tyr Glu Met Leu     #               195     - Ala Gly Gln Ala Pro Phe Glu Gly Glu Asp Gl - #u Asp Glu Leu Phe     #               210     - Gln Ser Ile Met Glu His Asn Val Ala Tyr Pr - #o Lys Ser Met Ser     #               225     - Lys Glu Ala Val Ala Ile Cys Lys Gly Leu Me - #t Thr Lys His Pro     #               240     - Gly Lys Arg Leu Gly Cys Gly Pro Glu Gly Gl - #u Arg Asp Ile Lys     #               255     - Glu His Ala Phe Phe Arg Tyr Ile                     260     - (2) INFORMATION FOR SEQ ID NO:14:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 269     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 15 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14:     - Ser Asp Phe Ser Phe Leu Met Val Leu Gly Ly - #s Gly Ser Phe Gly     #               015     - Lys Val Met Leu Ala Glu Arg Arg Gly Ser As - #p Glu Leu Tyr Ala     #               030     - Ile Lys Ile Leu Lys Lys Asp Val Ile Val Gl - #n Asp Asp Asp Val     #               045     - Asp Cys Thr Leu Val Glu Lys Arg Val Leu Al - #a Leu Gly Gly Arg     #               060     - Gly Pro Gly Gly Arg Pro His Phe Leu Thr Gl - #n Leu His Ser Thr     #               075     - Phe Gln Thr Pro Asp Arg Leu Tyr Phe Val Me - #t Glu Tyr Val Thr     #               090     - Gly Gly Asp Leu Met Tyr His Ile Gln Gln Le - #u Gly Lys Phe Lys     #               105     - Glu Pro His Ala Ala Phe Tyr Ala Ala Glu Il - #e Ala Ile Gly Leu     #               120     - Phe Phe Leu His Asn Gln Gly Ile Ile Tyr Ar - #g Asp Leu Lys Leu     #               135     - Asp Asn Val Met Leu Asp Ala Glu Gly His Il - #e Lys Ile Thr Asp     #               150     - Phe Gly Met Cys Lys Glu Asn Val Phe Pro Gl - #y Ser Thr Thr Arg     #               165     - Thr Phe Cys Gly Thr Pro Asp Tyr Ile Ala Pr - #o Glu Ile Ile Ala     #               180     - Tyr Gln Pro Tyr Gly Lys Ser Val Asp Trp Tr - #p Ser Phe Gly Val     #               195     - Leu Leu Tyr Glu Met Leu Ala Gly Gln Pro Pr - #o Phe Asp Gly Glu     #               210     - Asp Glu Glu Glu Leu Phe Gln Ala Ile Met Gl - #u Gln Thr Val Thr     #               225     - Tyr Pro Lys Ser Leu Ser Arg Glu Ala Val Al - #a Ile Cys Lys Gly     #               240     - Phe Leu Thr Lys His Pro Ala Lys Arg Leu Gl - #y Ser Gly Pro Asp     #               255     - Gly Glu Pro Thr Ile Arg Ala His Gly Phe Ph - #e Arg Trp Ile     #               265     - (2) INFORMATION FOR SEQ ID NO:15:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 264     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: bovine     #Column 16 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15:     - Thr Asp Phe Asn Phe Leu Met Val Leu Gly Ly - #s Gly Ser Phe Gly     #               015     - Lys Val Met Leu Ala Asp Arg Lys Gly Thr Gl - #u Glu Leu Tyr Ala     #               030     - Ile Lys Ile Leu Lys Lys Asp Val Val Ile Gl - #n Asp Asp Asp Val     #               045     - Glu Cys Thr Met Val Glu Lys Arg Val Leu Al - #a Leu Met Asp Lys     #               060     - Pro Pro Phe Leu Thr Gln Leu His Ser Cys Ph - #e Gln Thr Val Asp     #               075     - Arg Leu Tyr Phe Val Met Glu Tyr Val Asn Gl - #y Gly Asp Leu Met     #               090     - Tyr His Ile Gln Gln Val Gly Lys Phe Lys Gl - #u Pro Gln Ala Val     #               105     - Phe Tyr Ala Ala Glu Ile Ser Ile Gly Leu Ph - #e Phe Leu His Lys     #               120     - Arg Gly Ile Ile Tyr Arg Asp Leu Lys Leu As - #p Asn Val Met Leu     #               135     - Asp Ser Glu Gly His Ile Lys Ile Ala Asp Ph - #e Gly Met Cys Lys     #               150     - Glu His Met Met Asp Gly Val Thr Thr Arg Th - #r Phe Cys Gly Thr     #               165     - Pro Asp Tyr Ile Ala Pro Glu Ile Ile Ala Ty - #r Gln Pro Tyr Gly     #               180     - Lys Ser Val Asp Trp Trp Ala Tyr Gly Val Le - #u Leu Tyr Glu Met     #               195     - Leu Ala Gly Gln Pro Pro Phe Asp Gly Glu As - #p Glu Asp Glu Leu     #               210     - Phe Gln Ser Ile Met Glu His Asn Val Ser Ty - #r Pro Lys Ser Leu     #               225     - Ser Lys Glu Ala Val Ser Ile Cys Lys Gly Le - #u Met Thr Lys His     #               240     - Pro Ala Lys Arg Leu Gly Cys Gly Pro Glu Gl - #y Glu Arg Asp Val     #               255     - Arg Glu His Ala Phe Phe Arg Arg Ile                     260     - (2) INFORMATION FOR SEQ ID NO:16:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 269     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 17 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:16:     - Ser Asp Phe Ser Phe Leu Met Val Leu Gly Ly - #s Gly Ser Phe Gly     #               015     - Lys Val Met Leu Ala Glu Arg Arg Gly Ser As - #p Glu Leu Tyr Ala     #               030     - Ile Lys Ile Leu Lys Lys Asp Val Ile Val Gl - #n Asp Asp Asp Val     #               045     - Asp Cys Thr Leu Val Glu Lys Arg Val Leu Al - #a Leu Gly Gly Arg     #               060     - Gly Pro Gly Gly Arg Pro His Phe Leu Thr Gl - #n Leu His Ser Thr     #               075     - Phe Gln Thr Pro Asp Arg Leu Tyr Glu Val Me - #t Glu Tyr Val Thr     #               090     - Gly Gly Asp Leu Met Tyr His Ile Gln Gln Le - #u Gly Lys Phe Lys     #               105     - Glu Pro His Ala Ala Phe Tyr Ala Ala Glu Il - #e Ala Ile Gly Leu     #               120     - Phe Phe Leu His Asn Gln Gly Ile Ile Tyr Ar - #g Asp Leu Lys Leu     #               135     - Asp Asn Val Met Leu Asp Ala Glu Gly His Il - #e Lys Ile Thr Asp     #               150     - Phe Gly Met Cys Lys Glu Asn Val Phe Pro Gl - #y Ser Thr Thr Arg     #               165     - Thr Phe Cys Gly Thr Pro Asp Tyr Ile Ala Pr - #o Glu Ile Ile Ala     #               180     - Tyr Gln Pro Tyr Asp Lys Ser Val Asp Trp Tr - #p Ala Phe Gly Val     #               195     - Leu Leu Tyr Glu Met Leu Ala Gly Gln Pro Pr - #o Phe Asp Gly Glu     #               210     - Asp Glu Glu Glu Leu Phe Gln Ala Ile Met Gl - #u Gln Thr Val Thr     #               225     - Tyr Pro Lys Ser Leu Ser Arg Glu Ala Val Al - #a Ile Cys Lys Gly     #               240     - Phe Leu Thr Lys His Pro Gly Lys Arg Leu Gl - #y Ser Gly Pro Asp     #               255     - Gly Glu Pro Thr Ile Arg Ala His Gly Phe Ph - #e Arg Asn Ile     #               265     - (2) INFORMATION FOR SEQ ID NO:17:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 264     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:     #melanogaster ORGANISM: Drosophila     #Column 18 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:17:     - Thr Asp Phe Asn Phe Ile Lys Val Leu Gly Ly - #s Gly Ser Phe Gly     #               015     - Lys Val Leu Leu Ala Glu Arg Lys Gly Ser Gl - #u Glu Leu Tyr Ala     #               030     - Ile Lys Ile Leu Lys Lys Asp Val Ile Ile Gl - #n Asp Asp Asp Val     #               045     - Glu Cys Thr Met Ile Glu Lys Arg Val Leu Al - #a Leu Gly Glu Lys     #               060     - Pro Pro Phe Leu Val Gln Leu His Ser Cys Ph - #e Gln Thr Met Asp     #               075     - Arg Leu Phe Phe Val Met Glu Tyr Val Asn Gl - #y Gly Asp Leu Ile     #               090     - Phe Gln Ile Gln Gln Phe Gly Lys Phe Lys Gl - #u Pro Val Ala Val     #               105     - Phe Tyr Ala Ala Glu Ile Ala Ala Gly Leu Ph - #e Phe Leu His Thr     #               120     - Lys Gly Ile Leu Tyr Arg Asp Leu Lys Leu As - #p Asn Val Leu Leu     #               135     - Asp Ala Asp Gly His Val Lys Ile Ala Asp Ph - #e Gly Met Cys Lys     #               150     - Glu Asn Ile Val Gly Asp Lys Thr Thr Lys Th - #r Phe Cys Gly Thr     #               165     - Pro Asp Tyr Ile Ala Pro Glu Ile Ile Leu Ty - #r Gln Pro Tyr Gly     #               180     - Lys Ser Val Asp Trp Trp Ala Tyr Gly Val Le - #u Leu Tyr Glu Met     #               195     - Leu Val Gly Gln Pro Pro Phe Asp Gly Glu As - #p Glu Glu Glu Leu     #               210     - Phe Ala Ala Ile Thr Asp His Asn Val Ser Ty - #r Pro Lys Ser Leu     #               225     - Ser Lys Glu Ala Lys Glu Ala Cys Lys Gly Ph - #e Leu Thr Lys Gln     #               240     - Pro Asn Lys Arg Leu Gly Cys Gly Ser Ser Gl - #y Glu Glu Asp Val     #               255     - Arg Leu His Pro Phe Ser Arg Arg Ile                     260     - (2) INFORMATION FOR SEQ ID NO:18:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 264     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: rat     #Column 20 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:18:     - Glu Glu Tyr Gln Leu Phe Glu Glu Leu Gly Ly - #s Gly Ala Phe Ser     #               015     - Val Val Arg Arg Cys Val Lys Val Leu Ala Gl - #y Gln Glu Tyr Ala     #               030     - Ala Lys Ile Ile Asn Thr Lys Lys Leu Ser Al - #a Arg Asp His Gln     #               045     - Lys Leu Glu Arg Glu Ala Arg Ile Cys Arg Le - #u Leu Lys His Pro     #               060     - Asn Ile Val Arg Leu His Asp Ser Ile Ser Gl - #u Glu Gly His His     #               075     - Tyr Leu Ile Phe Asp Leu Val Thr Gly Gly Gl - #u Leu Phe Glu Asp     #               090     - Ile Val Ala Arg Glu Tyr Tyr Ser Glu Ala As - #p Ala Ser His Cys     #               105     - Ile Gln Gln Ile Leu Glu Ala Val Leu His Cy - #s His Gln Met Gly     #               120     - Val Val His Arg Asp Leu Lys Pro Glu Asn Le - #u Leu Leu Ala Ser     #               135     - Lys Leu Lys Gly Ala Ala Val Lys Leu Ala As - #p Phe Gly Leu Ala     #               150     - Ile Glu Val Glu Gly Glu Gln Gln Ala Trp Ph - #e Gly Phe Ala Gly     #               165     - Thr Pro Gly Tyr Leu Ser Pro Glu Val Leu Ar - #g Lys Asp Pro Tyr     #               180     - Gly Lys Pro Val Asp Leu Trp Ala Cys Gly Va - #l Ile Leu Tyr Ile     #               195     - Leu Leu Val Gly Tyr Pro Pro Phe Trp Asp Gl - #u Asp Gln His Arg     #               210     - Leu Tyr Gln Gln Ile Lys Ala Gly Ala Tyr As - #p Phe Pro Ser Pro     #               225     - Glu Trp Asp Thr Val Thr Pro Glu Ala Lys As - #p Leu Ile Asn Lys     #               240     - Met Leu Thr Ile Asn Pro Ser Lys Arg Ile Th - #r Ala Ala Glu Ala     #               255     - Leu Lys His Pro Trp Ile Ser His Arg                     260     - (2) INFORMATION FOR SEQ ID NO:19:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 264     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: rat     #Column 21 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:19:     - Asp Glu Tyr Gln Leu Tyr Glu Asp Ile Gly Ly - #s Gly Ala Phe Ser     #               015     - Val Val Arg Arg Cys Val Lys Leu Cys Thr Gl - #y His Glu Tyr Ala     #               030     - Ala Lys Ile Ile Asn Thr Lys Lys Leu Ser Al - #a Arg Asp His Gln     #               045     - Lys Leu Glu Arg Glu Ala Arg Ile Cys Arg Le - #u Leu Lys His Ser     #               060     - Asn Ile Val Arg Leu His Asp Ser Ile Ser Gl - #u Glu Gly Phe His     #               075     - Tyr Leu Val Phe Asp Leu Val Thr Gly Gly Gl - #u Leu Phe Glu Asp     #               090     - Ile Val Ala Arg Glu Tyr Tyr Ser Glu Ala As - #p Ala Ser His Cys     #               105     - Ile Gln Gln Ile Leu Glu Ala Val Leu His Cy - #s His Gln Met Gly     #               120     - Val Val His Arg Asp Leu Lys Pro Glu Asn Le - #u Leu Leu Ala Ser     #               135     - Lys Leu Lys Gly Ala Ala Val Lys Leu Ala As - #p Phe Gly Leu Ala     #               150     - Ile Glu Val Gln Gly Asp Gln Gln Ala Trp Ph - #e Gly Phe Ala Gly     #               165     - Thr Pro Gly Tyr Leu Ser Pro Glu Val Leu Ar - #g Lys Glu Ala Tyr     #               180     - Gly Lys Pro Val Asp Ile Trp Ala Cys Gly Va - #l Ile Leu Tyr Ile     #               195     - Leu Leu Val Gly Tyr Pro Pro Phe Trp Asp Gl - #u Asp Gln His Lys     #               210     - Leu Tyr Gln Gln Ile Lys Ala Gly Ala Tyr As - #p Phe Pro Ser Pro     #               225     - Glu Trp Asp Thr Val Thr Pro Glu Ala Lys As - #n Leu Ile Asn Gln     #               240     - Met Leu Thr Ile Asn Pro Ala Lys Arg Ile Th - #r Ala His Glu Ala     #               255     - Leu Lys His Pro Trp Val Cys Gln Arg                     260     - (2) INFORMATION FOR SEQ ID NO:20:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 268     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: rabbit     #8 Column 22EATURE: Protein kinase; 822 Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:20:     - Glu Asn Tyr Glu Pro Lys Glu Ile Leu Gly Ar - #g Gly Val Ser Ser     #               015     - Val Val Arg Arg Cys Ile His Lys Pro Thr Cy - #s Lys Glu Tyr Ala     #               030     - Val Lys Ile Ile Asp Val Thr Gly Gly Gly Se - #r Phe Ser Ala Glu     #               045     - Leu Arg Glu Ala Thr Leu Lys Glu Val Asp Il - #e Leu Arg Lys Val     #               060     - Ser Gly His Pro Asn Ile Ile Gln Leu Lys As - #p Thr Tyr Glu Thr     #               075     - Asn Thr Phe Phe Phe Leu Val Phe Asp Leu Me - #t Lys Lys Gly Glu     #               090     - Leu Phe Asp Tyr Leu Thr Glu Lys Val Thr Le - #u Ser Glu Lys Glu     #               105     - Thr Arg Lys Ile Met Arg Ala Leu Leu Glu Va - #l Ile Cys Ala Leu     #               120     - His Lys Leu Asn Ile Val His Arg Asp Leu Ly - #s Pro Glu Asn Ile     #               135     - Leu Leu Asp Asp Asp Met Asn Ile Lys Leu Th - #r Asp Phe Gly Phe     #               150     - Ser Cys Gln Leu Asp Pro Gly Glu Lys Leu Ar - #g Glu Val Cys Gly     #               165     - Thr Pro Ser Tyr Leu Ala Pro Glu Ile Ile Gl - #u Cys Ser Met Asn     #               180     - His Pro Gly Tyr Gly Lys Glu Val Asp Met Tr - #p Ser Thr Gly Val     #               195     - Ile Met Tyr Thr Leu Leu Ala Gly Ser Pro Pr - #o Phe Trp His Arg     #               210     - Lys Gln Met Leu Met Leu Arg Met Ile Met Se - #r Gly Asn Tyr Gln     #               225     - Phe Gly Ser Pro Glu Trp Asp Asp Tyr Ser As - #p Thr Val Lys Asp     #               240     - Leu Val Ser Arg Phe Leu Val Val Gln Pro Gl - #n Lys Arg Tyr Thr     #               255     - Ala Glu Glu Ala Leu Ala His Pro Phe Phe Gl - #n Gln Tyr     #               265     - (2) INFORMATION FOR SEQ ID NO:21:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 268     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: mouse     #Column 23 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:21:     - Glu Asn Tyr Glu Pro Lys Glu Ile Leu Gly Ar - #g Gly Val Ser Ser     #               015     - Val Val Arg Arg Cys Ile His Lys Pro Thr Cy - #s Gln Glu Tyr Ala     #               030     - Val Lys Ile Ile Asp Ile Thr Gly Gly Gly Se - #r Phe Ser Ser Glu     #               045     - Leu Arg Glu Ala Thr Leu Lys Glu Val Asp Il - #e Leu Gln Lys Val     #               060     - Ser Gly His His Asn Ile Ile Gln Leu Lys As - #p Thr Tyr Glu Thr     #               075     - Asn Thr Phe Phe Phe Leu Val Phe Asp Leu Me - #t Lys Arg Gly Glu     #               090     - Leu Phe Asp Tyr Leu Thr Glu Lys Val Thr Le - #u Thr Glu Lys Glu     #               105     - Thr Arg Lys Ile Met Arg Ala Leu Leu Glu Va - #l Ile Cys Thr Leu     #               120     - His Lys Leu Asn Ile Val His Arg Asp Leu Ly - #s Pro Glu Asn Ile     #               135     - Leu Leu Asp Asp Asn Met Asn Ile Lys Leu Th - #r Asp Phe Gly Phe     #               150     - Ser Cys Gln Leu Gln Pro Gly Glu Lys Leu Ar - #g Glu Val Cys Gly     #               165     - Thr Pro Ser Tyr Leu Ala Pro Glu Ile Ile Gl - #n Cys Ser Met Gly     #               180     - His Pro Gly Tyr Gly Lys Glu Val Asp Met Tr - #p Ser Thr Gly Val     #               195     - Ile Met Tyr Thr Leu Leu Ala Gly Ser Pro Pr - #o Phe Trp His Arg     #               210     - Lys Gln Met Leu Met Leu Arg Met Ile Met As - #p Gly Lys Tyr Gln     #               225     - Phe Gly Ser Pro Glu Trp Asp Asp Tyr Ser As - #p Thr Val Lys Asp     #               240     - Leu Val Ser Arg Phe Leu Val Val Gln Pro Gl - #n Asp Arg Cys Ser     #               255     - Ala Glu Glu Ala Leu Ala His Pro Phe Phe Gl - #n Glu Tyr     #               265     - (2) INFORMATION FOR SEQ ID NO:22:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 261     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: rabbit     #Column 24 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:22:     - Phe Ser Met Asn Ser Lys Glu Ala Leu Gly Gl - #y Gly Lys Phe Gly     #               015     - Ala Val Cys Thr Cys Thr Glu Lys Ser Thr Gl - #y Leu Lys Leu Ala     #               030     - Ala Lys Val Ile Lys Lys Gln Thr Pro Lys As - #p Lys Glu Met Val     #               045     - Met Leu Glu Ile Glu Val Met Asn Gln Leu As - #n His Arg Asn Leu     #               060     - Ile Gln Leu Tyr Ala Ala Ile Glu Thr Pro Hi - #s Glu Ile Val Leu     #               075     - Phe Met Glu Tyr Ile Glu Gly Gly Glu Leu Ph - #e Glu Arg Ile Val     #               090     - Asp Glu Asp Tyr His Leu Thr Glu Val Asp Th - #r Met Val Phe Val     #               105     - Arg Gln Ile Cys Asp Gly Ile Leu Phe Met Hi - #s Lys Met Arg Val     #               120     - Leu His Leu Asp Leu Lys Pro Glu Asn Ile Le - #u Cys Val Asn Thr     #               135     - Thr Gly His Leu Val Lys Ile Ile Asp Phe Gl - #y Leu Ala Arg Arg     #               150     - Tyr Asn Pro Asn Glu Lys Leu Lys Val Asn Ph - #e Gly Thr Pro Glu     #               165     - Phe Leu Ser Pro Glu Val Val Asn Tyr Asp Gl - #n Ile Ser Asp Lys     #               180     - Thr Asp Met Trp Ser Leu Gly Val Ile Thr Ty - #r Met Leu Leu Ser     #               195     - Gly Leu Ser Pro Phe Leu Gly Asp Asp Asp Th - #r Glu Thr Leu Asn     #               210     - Asn Val Leu Ser Gly Asn Trp Tyr Phe Asp Gl - #u Glu Thr Phe Glu     #               225     - Ala Val Ser Asp Glu Ala Lys Asp Phe Val Se - #r Asn Leu Ile Val     #               240     - Lys Glu Gln Gly Ala Arg Met Ser Ala Ala Gl - #n Cys Leu Ala His     #               255     - Pro Trp Leu Asn Asn Leu                     260     - (2) INFORMATION FOR SEQ ID NO:23:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 260     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: chicken     #Column 25 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:23:     - Asp Val Tyr Asn Ile Glu Glu Arg Leu Gly Se - #r Gly Lys Phe Gly     #               015     - Gln Val Phe Arg Leu Val Glu Lys Lys Thr Gl - #y Lys Val Trp Ala     #               030     - Gly Lys Phe Phe Lys Ala Tyr Ser Ala Lys Gl - #u Lys Glu Asn Ile     #               045     - Arg Asp Glu Ile Ser Ile Met Asn Cys Leu Hi - #s His Pro Lys Leu     #               060     - Val Gln Cys Val Asp Ala Phe Glu Glu Lys Al - #a Asn Ile Val Met     #               075     - Val Leu Glu Met Val Ser Gly Gly Glu Leu Ph - #e Glu Arg Ile Ile     #               090     - Asp Glu Asp Phe Leu Thr Glu Arg Glu Cys Il - #e Lys Tyr Met Arg     #               105     - Gln Ile Ser Glu Gly Val Glu Tyr Ile His Ly - #s Gln Gly Ile Val     #               120     - His Leu Asp Leu Lys Pro Glu Asn Ile Met Cy - #s Val Asn Lys Thr     #               135     - Gly Thr Ser Ile Lys Leu Ile Asp Phe Gly Le - #u Ala Arg Arg Leu     #               150     - Glu Ser Ala Gly Ser Leu Lys Val Leu Phe Gl - #y Thr Pro Glu Phe     #               165     - Val Ala Pro Glu Val Ile Asn Tyr Glu Pro Il - #e Gly Tyr Glu Thr     #               180     - Asp Met Trp Ser Ile Gly Val Ile Cys Tyr Il - #e Leu Val Ser Gly     #               195     - Leu Ser Pro Phe Met Gly Asp Asn Asp Asn Gl - #u Thr Leu Ala Asn     #               210     - Val Thr Ser Ala Thr Trp Thr Phe Asp Asp Gl - #u Ala Phe Asp Glu     #               225     - Ile Ser Asp Asp Ala Lys Asp Phe Ile Ser As - #n Leu Leu Lys Lys     #               240     - Asp Met Lys Ser Arg Leu Asn Cys Thr Gln Cy - #s Leu Gln His Pro     #               255     - Trp Leu Gln Lys Asp                     260     - (2) INFORMATION FOR SEQ ID NO:24:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 264     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 26 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:24:     - Ala Lys Tyr Asp Ile Lys Ala Leu Ile Gly Ar - #g Gly Ser Phe Ser     #               015     - Arg Val Val Arg Val Glu His Arg Ala Thr Ar - #g Gln Pro Tyr Ala     #               030     - Ile Lys Met Ile Glu Thr Lys Tyr Arg Glu Gl - #y Arg Glu Val Cys     #               045     - Glu Ser Glu Leu Arg Val Leu Arg Arg Val Ar - #g His Ala Asn Ile     #               060     - Ile Gln Leu Val Glu Val Phe Glu Thr Gln Gl - #u Arg Val Tyr Met     #               075     - Val Met Glu Leu Ala Thr Gly Gly Glu Leu Ph - #e Asp Arg Ile Ile     #               090     - Ala Lys Gly Ser Glu Phe Thr Glu Arg Asp Al - #a Thr Arg Val Leu     #               105     - Gln Met Val Leu Asp Gly Val Arg Tyr Leu Hi - #s Ala Leu Gly Ile     #               120     - Thr His Arg Asp Leu Lys Pro Glu Asn Leu Le - #u Tyr Tyr His Pro     #               135     - Gly Thr Asp Ser Lys Ile Ile Ile Thr Asp Ph - #e Gly Leu Ala Ser     #               150     - Ala Arg Lys Lys Gly Asp Asp Cys Leu Met Ly - #s Thr Thr Cys Gly     #               165     - Thr Pro Glu Tyr Ile Ala Pro Glu Val Leu Va - #l Arg Lys Pro Tyr     #               180     - Thr Asn Ser Val Asp Met Trp Ala Leu Gly Va - #l Ile Ala Tyr Ile     #               195     - Leu Leu Ser Gly Thr Met Pro Phe Glu Asp As - #p Asn Arg Thr Arg     #               210     - Leu Tyr Arg Gln Ile Leu Arg Gly Lys Tyr Se - #r Tyr Ser Gly Glu     #               225     - Pro Trp Pro Ser Val Ser Asn Leu Ala Lys As - #p Phe Ile Asp Arg     #               240     - Leu Leu Thr Val Asp Pro Gly Ala Arg Met Th - #r Ala Leu Gln Ala     #               255     - Leu Arg His Pro Trp Val Val Ser Met                     260     - (2) INFORMATION FOR SEQ ID NO:25:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 257     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Saccharomyce - #s cerevisiae     #Column 28 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:25:     - Gly Asn Tyr Gln Ile Val Lys Thr Leu Gly Gl - #u Gly Ser Phe Gly     #               015     - Lys Val Lys Leu Ala Tyr His Thr Thr Thr Gl - #y Gln Lys Val Ala     #               030     - Leu Lys Ile Ile Asn Lys Lys Val Leu Ala Ly - #s Ser Asp Met Gln     #               045     - Gly Arg Ile Glu Arg Glu Ile Ser Tyr Leu Ar - #g Leu Leu Arg His     #               060     - Pro His Ile Ile Lys Leu Tyr Asp Val Ile Ly - #s Ser Lys Asp Glu     #               075     - Ile Ile Met Val Ile Glu Tyr Ala Gly Asn Gl - #u Leu Phe Asp Tyr     #               090     - Ile Val Gln Arg Asp Lys Met Ser Glu Gln Gl - #u Ala Arg Arg Phe     #               105     - Phe Gln Gln Ile Ile Ser Ala Val Glu Tyr Cy - #s His Arg His Lys     #               120     - Ile Val His Arg Asp Leu Lys Pro Glu Asn Le - #u Leu Leu Asp Glu     #               135     - His Leu Asn Val Lys Ile Ala Asp Phe Gly Le - #u Ser Asn Ile Met     #               150     - Thr Asp Gly Asn Phe Leu Lys Thr Ser Cys Gl - #y Ser Pro Asn Tyr     #               165     - Ala Ala Pro Glu Val Ile Ser Gly Lys Leu Ty - #r Ala Gly Pro Glu     #               180     - Val Asp Val Trp Ser Cys Gly Val Ile Leu Ty - #r Val Met Leu Cys     #               195     - Arg Arg Leu Pro Phe Asp Asp Glu Ser Ile Pr - #o Val Leu Phe Lys     #               210     - Asn Ile Ser Asn Gly Val Tyr Thr Leu Pro Ly - #s Phe Leu Ser Pro     #               225     - Gly Ala Ala Gly Leu Ile Lys Arg Met Leu Il - #e Val Asn Pro Leu     #               240     - Asn Arg Ile Ser Ile His Glu Ile Met Gln As - #p Asp Trp Phe Lys     #               255     - Val Asp     - (2) INFORMATION FOR SEQ ID NO:26:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 252     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Schizosaccha - #romyces pombe     #Column 29 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:26:     - Gly Val Trp Arg Leu Gly Lys Thr Leu Gly Th - #r Gly Ser Thr Ser     #               015     - Cys Val Arg Leu Ala Lys His Ala Lys Thr Gl - #y Asp Leu Ala Ala     #               030     - Ile Lys Ile Ile Pro Ile Arg Tyr Ala Ser Il - #e Gly Met Glu Ile     #               045     - Leu Met Met Arg Leu Leu Arg His Pro Asn Il - #e Leu Arg Leu Tyr     #               060     - Asp Val Trp Thr Asp His Gln His Met Tyr Le - #u Ala Leu Glu Tyr     #               075     - Val Pro Asp Gly Glu Leu Phe His Tyr Ile Ar - #g Lys His Gly Pro     #               090     - Leu Ser Glu Arg Glu Ala Ala His Tyr Leu Se - #r Gln Ile Leu Asp     #               105     - Ala Val Ala His Cys His Arg Phe Arg Phe Ar - #g His Arg Asp Leu     #               120     - Lys Leu Glu Asn Ile Leu Ile Lys Val Asn Gl - #u Gln Gln Ile Lys     #               135     - Ile Ala Asp Phe Gly Met Ala Thr Val Glu Pr - #o Asn Asp Ser Cys     #               150     - Leu Glu Asn Tyr Cys Gly Ser Leu His Tyr Le - #u Ala Pro Glu Ile     #               165     - Val Ser His Lys Pro Tyr Arg Gly Ala Pro Al - #a Asp Val Trp Ser     #               180     - Cys Gly Val Ile Leu Tyr Ser Leu Leu Ser As - #n Lys Leu Pro Phe     #               195     - Gly Gly Gln Asn Thr Asp Val Ile Tyr Asn Ly - #s Ile Arg His Gly     #               210     - Ala Tyr Asp Leu Pro Ser Ser Ile Ser Ser Al - #a Ala Gln Asp Leu     #               225     - Leu His Arg Met Leu Asp Val Asn Pro Ser Th - #r Arg Ile Thr Ile     #               240     - Pro Glu Phe Phe Ser His Pro Phe Leu Met Gl - #y Cys     #               250     - (2) INFORMATION FOR SEQ ID NO:27:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 260     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Saccharomyce - #s cerevisiae     #Column 30 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:27:     - Gly Asp Trp Glu Phe Val Glu Thr Val Gly Al - #a Gly Ser Met Gly     #               015     - Lys Val Lys Leu Ala Lys His Arg Tyr Thr As - #n Glu Val Cys Ala     #               030     - Val Lys Ile Val Asn Arg Ala Thr Lys Ala Ph - #e Leu His Lys Glu     #               045     - Arg Asp Lys Arg Thr Ile Arg Glu Ala Ser Le - #u Gly Gln Ile Leu     #               060     - Tyr His Pro His Ile Cys Arg Leu Phe Glu Me - #t Cys Thr Leu Ser     #               075     - Asn His Phe Tyr Met Leu Phe Glu Tyr Val Se - #r Gly Gly Gln Leu     #               090     - Leu His Tyr Ile Ile Gln His Gly Ser Ile Ar - #g Glu His Gln Ala     #               105     - Arg Lys Phe Ala Arg Gly Ile Ala Ser Ala Le - #u Ile Tyr Leu His     #               120     - Ala Asn Asn Ile Val His Arg Asp Leu Lys Il - #e Glu Asn Ile Met     #               135     - Ile Ser Asp Ser Ser Glu Ile Lys Ile Ile As - #p Phe Gly Leu Ser     #               150     - Asn Ile Tyr Asp Ser Arg Lys Gln Leu His Th - #r Phe Cys Gly Ser     #               165     - Leu Tyr Phe Ala Ala Pro Glu Leu Leu Lys Al - #a Asn Pro Tyr Thr     #               180     - Gly Pro Glu Val Asp Val Trp Ser Phe Gly Va - #l Val Leu Phe Val     #               195     - Leu Val Cys Gly Lys Val Pro Phe Asp Asp Gl - #u Asn Ser Ser Val     #               210     - Leu His Glu Lys Ile Lys Gln Gly Lys Val Gl - #u Tyr Pro Gln His     #               225     - Leu Ser Ile Glu Val Ile Ser Leu Leu Ser Ly - #s Met Leu Val Val     #               240     - Asp Pro Lys Arg Arg Ala Thr Leu Lys Gln Va - #l Val Glu His His     #               255     - Trp Met Val Arg Gly                     260     - (2) INFORMATION FOR SEQ ID NO:28:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 260     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Saccharomyce - #s cerevisiae     #Column 31 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:28:     - Gly Asp Trp Glu Phe Leu Glu Thr Val Gly Al - #a Gly Ser Met Gly     #               015     - Lys Val Lys Leu Val Lys His Arg Gln Thr Ly - #s Glu Ile Cys Val     #               030     - Ile Lys Ile Val Asn Arg Ala Ser Lys Ala Ty - #r Leu His Lys Gln     #               045     - Arg Asp Lys Arg Thr Val Arg Glu Ala Ser Le - #u Gly Gln Ile Leu     #               060     - Tyr His Pro His Ile Cys Arg Leu Phe Glu Me - #t Cys Thr Met Ser     #               075     - Asn His Phe Tyr Met Leu Phe Glu Tyr Val Se - #r Gly Gly Gln Leu     #               090     - Leu His Tyr Ile Ile Gln His Gly Ser Leu Ly - #s Glu His His Ala     #               105     - Arg Lys Phe Ala Arg Gly Ile Ala Ser Ala Le - #u Gln Tyr Leu His     #               120     - Ala Asn Asn Ile Val His Arg Asp Leu Lys Il - #e Glu Asn Ile Met     #               135     - Ile Ser Ser Ser Gly Glu Ile Lys Ile Ile As - #p Phe Gly Leu Ser     #               150     - Asn Ile Phe Asp Tyr Arg Lys Gln Leu His Th - #r Phe Cys Gly Ser     #               165     - Leu Tyr Phe Ala Ala Pro Glu Leu Leu Lys Al - #a Gln Pro Tyr Thr     #               180     - Gly Pro Glu Val Asp Ile Trp Ser Phe Gly Il - #e Val Leu Tyr Val     #               195     - Leu Val Cys Gly Lys Val Pro Phe Asp Asp Gl - #u Asn Ser Ser Ile     #               210     - Leu His Glu Lys Ile Lys Lys Gly Lys Val As - #p Tyr Pro Ser His     #               225     - Leu Ser Ile Glu Val Ile Ser Leu Leu Thr Ar - #g Met Ile Val Val     #               240     - Asp Pro Leu Arg Arg Ala Thr Leu Lys Asn Va - #l Val Glu His Pro     #               255     - Trp Met Asn Arg Gly                     260     - (2) INFORMATION FOR SEQ ID NO:29:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 274     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Saccharomyce - #s cerevisiae     #Column 33 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:29:     - Ala Asn Tyr Lys Arg Leu Glu Lys Val Gly Gl - #u Gly Thr Tyr Gly     #               015     - Val Val Tyr Lys Ala Leu Asp Leu Arg Pro Gl - #y Gln Gly Gln Arg     #               030     - Val Val Ala Leu Lys Lys Ile Arg Leu Glu Se - #r Glu Asp Glu Gly     #               045     - Val Pro Ser Thr Ala Ile Arg Glu Ile Ser Le - #u Leu Lys Glu Leu     #               060     - Lys Asp Asp Asn Ile Val Arg Leu Tyr Asp Il - #e Val His Ser Asp     #               075     - Ala His Lys Leu Tyr Leu Val Phe Glu Phe Le - #u Asp Leu Asp Leu     #               090     - Lys Arg Tyr Met Glu Gly Ile Pro Lys Asp Gl - #n Pro Leu Gly Ala     #               105     - Asp Ile Val Lys Lys Phe Met Met Gln Leu Cy - #s Lys Gly Ile Ala     #               120     - Tyr Cys His Ser His Arg Ile Leu His Arg As - #p Leu Lys Pro Gln     #               135     - Asn Leu Leu Ile Asn Lys Asp Gly Asn Leu Ly - #s Leu Gly Asp Phe     #               150     - Gly Leu Ala Arg Ala Phe Gly Val Pro Leu Ar - #g Ala Tyr Thr His     #               165     - Glu Ile Val Thr Leu Trp Tyr Arg Ala Pro Gl - #u Val Leu Leu Gly     #               180     - Gly Lys Gln Tyr Ser Thr Gly Val Asp Ile Tr - #p Ser Ile Gly Cys     #               195     - Ile Phe Ala Glu Met Cys Asn Arg Lys Pro Il - #e Phe Ser Gly Asp     #               210     - Ser Glu Ile Asp Gln Ile Phe Lys Ile Phe Ar - #g Val Leu Gly Thr     #               225     - Pro Asn Glu Ala Ile Trp Pro Asp Ile Val Ty - #r Leu Pro Asp Leu     #               240     - Asp Pro Arg Gly Ile Asp Leu Leu Asp Lys Le - #u Leu Ala Tyr Asp     #               255     - Pro Ile Asn Arg Ile Ser Ala Arg Arg Ala Al - #a Ile His Pro Tyr     #               270     - Phe Gln Glu Ser     - (2) INFORMATION FOR SEQ ID NO:30:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 274     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Schizoacchar - #omyces pombe     #Column 34 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:30:     - Glu Asn Tyr Gln Lys Val Glu Lys Ile Gly Gl - #u Gly Thr Tyr Gly     #               015     - Val Val Tyr Lys Ala Arg His Lys Leu Ser Gl - #y Arg Ile Val Ala     #               030     - Met Lys Lys Ile Arg Leu Glu Asp Glu Ser Gl - #u Gly Val Pro Ser     #               045     - Thr Ala Ile Arg Glu Ile Ser Leu Leu Lys Gl - #u Val Asn Asp Glu     #               060     - Asn Asn Arg Ser Asn Cys Val Arg Leu Leu As - #p Ile Leu His Ala     #               075     - Glu Ser Lys Leu Tyr Leu Val Phe Glu Phe Le - #u Asp Met Asp Leu     #               090     - Lys Lys Tyr Met Asp Arg Ile Ser Glu Thr Gl - #y Ala Leu Asp Pro     #               105     - Arg Leu Val Gln Lys Phe Thr Tyr Gln Leu Va - #l Asn Gly Val Asn     #               120     - Phe Cys His Ser Arg Arg Ile Ile His Arg As - #p Leu Lys Pro Gln     #               135     - Asn Leu Leu Ile Asp Lys Glu Gly Asn Leu Ly - #s Leu Ala Asp Phe     #               150     - Gly Leu Ala Arg Ser Phe Gly Val Pro Leu Ar - #g Asn Tyr Thr His     #               165     - Glu Ile Val Thr Leu Trp Tyr Arg Ala Pro Gl - #u Val Leu Leu Gly     #               180     - Ser Arg His Tyr Ser Thr Gly Val Asp Ile Tr - #p Ser Val Gly Cys     #               195     - Ile Phe Ala Glu Met Ile Arg Arg Ser Pro Le - #u Phe Pro Gly Asp     #               210     - Ser Glu Ile Asp Glu Ile Phe Lys Ile Phe Gl - #n Val Leu Gly Thr     #               225     - Pro Asn Glu Glu Val Trp Pro Gly Val Thr Le - #u Leu Gln Asp Gly     #               240     - Glu Glu Asp Ala Ile Glu Leu Leu Ser Ala Me - #t Leu Val Tyr Asp     #               255     - Pro Ala His Arg Ile Ser Ala Lys Arg Ala Le - #u Gln Gln Asn Tyr     #               270     - Leu Arg Asp Phe     - (2) INFORMATION FOR SEQ ID NO:31:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 270     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 35 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:31:     - Glu Asp Tyr Thr Lys Ile Glu Lys Ile Gly Gl - #u Gly Thr Tyr Gly     #               015     - Val Val Tyr Lys Gly Arg His Lys Thr Thr Gl - #y Gln Val Val Ala     #               030     - Met Lys Lys Ile Arg Leu Glu Ser Glu Glu Gl - #u Gly Val Pro Ser     #               045     - Thr Ala Ile Arg Glu Ile Ser Leu Leu Lys Gl - #u Leu Arg His Pro     #               060     - Asn Ile Val Ser Leu Gln Asp Val Leu Met Gl - #n Asp Ser Arg Leu     #               075     - Tyr Leu Ile Phe Glu Phe Leu Ser Met Asp Le - #u Lys Lys Tyr Leu     #               090     - Asp Ser Ile Pro Pro Gly Gln Tyr Met Asp Se - #r Ser Leu Val Lys     #               105     - Ser Tyr Leu Tyr Gln Ile Leu Gln Gly Ile Va - #l Phe Cys His Ser     #               120     - Arg Arg Val Leu His Arg Asp Leu Lys Pro Gl - #n Asn Leu Leu Ile     #               135     - Asp Asp Lys Gly Thr Ile Lys Leu Ala Asp Ph - #e Gly Leu Ala Arg     #               150     - Ala Phe Gly Ile Pro Ile Arg Val Tyr Thr Hi - #s Glu Val Val Thr     #               165     - Leu Trp Tyr Arg Ser Pro Glu Val Leu Leu Gl - #y Ser Ala Arg Tyr     #               180     - Ser Thr Pro Val Asp Ile Trp Ser Ile Gly Th - #r Ile Phe Ala Glu     #               195     - Leu Ala Thr Lys Lys Pro Leu Phe His Gly As - #p Ser Glu Ile Asp     #               210     - Gln Leu Phe Arg Ile Phe Arg Ala Leu Gly Th - #r Pro Asn Asn Glu     #               225     - Val Trp Pro Glu Val Glu Ser Leu Gln Asp Le - #u Asp Glu Asn Gly     #               240     - Leu Asp Leu Leu Ser Lys Met Leu Ile Tyr As - #p Pro Ala Lys Arg     #               255     - Ile Ser Gly Lys Met Ala Leu Asn His Pro Ty - #r Phe Asn Asp Leu     #               270     - (2) INFORMATION FOR SEQ ID NO:32:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 278     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 36 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:32:     - Ser Arg Tyr Glu Pro Val Ala Glu Ile Gly Va - #l Gly Ala Tyr Gly     #               015     - Thr Val Tyr Lys Ala Arg Asp Pro His Ser Gl - #y His Phe Val Ala     #               030     - Leu Lys Ser Val Arg Val Pro Asn Gly Gly Gl - #y Gly Gly Gly Gly     #               045     - Leu Pro Ile Ser Thr Val Arg Glu Val Ala Le - #u Leu Arg Arg Leu     #               060     - Glu Ala Phe Glu His Pro Asn Val Val Arg Le - #u Met Asp Val Cys     #               075     - Ala Thr Ser Arg Thr Asp Arg Glu Ile Lys Va - #l Thr Leu Val Phe     #               090     - Glu His Val Asp Gln Asp Leu Arg Thr Tyr Le - #u Asp Lys Ala Pro     #               105     - Pro Pro Gly Leu Pro Ala Glu Thr Ile Lys As - #p Leu Met Arg Gln     #               120     - Phe Leu Arg Gly Leu Asp Phe Leu His Ala As - #n Cys Ile Val His     #               135     - Arg Asp Leu Lys Pro Glu Asn Ile Leu Val Th - #r Ser Gly Gly Thr     #               150     - Val Lys Leu Ala Asp Phe Gly Leu Ala Arg Il - #e Tyr Ser Tyr Gln     #               165     - Met Ala Leu Thr Pro Val Val Val Thr Leu Tr - #p Tyr Arg Ala Pro     #               180     - Glu Val Leu Leu Gln Ser Thr Tyr Ala Thr Pr - #o Val Asp Met Trp     #               195     - Ser Val Gly Cys Ile Phe Ala Glu Met Phe Ar - #g Arg Lys Pro Leu     #               210     - Phe Cys Gly Asn Ser Glu Ala Asp Gln Leu Gl - #y Lys Ile Phe Asp     #               225     - Leu Ile Gly Leu Pro Pro Glu Asp Asp Trp Pr - #o Arg Asp Val Ser     #               240     - Leu Pro Arg Met Glu Glu Ser Gly Ala Gln Le - #u Leu Leu Glu Met     #               255     - Leu Thr Phe Asn Pro His Lys Arg Ile Ser Al - #a Phe Arg Ala Leu     #               270     - Gln His Ser Tyr Leu His Lys Asp                     275     - (2) INFORMATION FOR SEQ ID NO:33:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 268     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Saccharomyce - #s cerevisiae     #Column 37 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:33:     - Met Glu Tyr Thr Lys Glu Lys Lys Val Gly Gl - #u Gly Thr Tyr Ala     #               015     - Val Val Tyr Leu Gly Cys Gln His Ser Thr Gl - #y Arg Lys Ile Ala     #               030     - Ile Lys Glu Ile Lys Thr Ser Glu Phe Lys As - #p Gly Leu Asp Met     #               045     - Ser Ala Ile Arg Glu Val Lys Tyr Leu Gln Gl - #u Met Gln His Pro     #               060     - Asn Val Ile Glu Leu Ile Asp Ile Phe Met Al - #a Tyr Asp Asn Leu     #               075     - Asn Leu Val Leu Glu Phe Leu Pro Thr Asp Le - #u Glu Val Val Ile     #               090     - Lys Asp Lys Ser Ile Leu Phe Thr Pro Ala As - #p Ile Lys Ala Trp     #               105     - Met Leu Met Thr Leu Arg Gly Val Tyr His Cy - #s His Arg Asn Phe     #               120     - Ile Leu His Arg Asp Leu Lys Pro Asn Asn Le - #u Leu Phe Ser Pro     #               135     - Asp Gly Gln Ile Lys Val Ala Asp Phe Gly Le - #u Ala Arg Ala Ile     #               150     - Pro Ala Pro His Glu Ile Leu Thr Ser Asn Va - #l Val Thr Arg Trp     #               165     - Tyr Arg Ala Pro Glu Leu Leu Phe Gly Ala Ly - #s His Tyr Thr Ser     #               180     - Ala Ile Asp Ile Trp Ser Val Gly Val Ile Ph - #e Ala Glu Leu Met     #               195     - Leu Arg Ile Pro Tyr Leu Pro Gly Gln Asn As - #p Val Asp Gln Met     #               210     - Glu Val Thr Phe Arg Ala Leu Gly Thr Pro Th - #r Asp Arg Asp Trp     #               225     - Pro Glu Val Ser Ser Phe Met Thr Ala Ser Gl - #u Tyr Ala Leu Asp     #               240     - Phe Met Cys Gly Met Leu Thr Met Asn Pro Gl - #n Lys Arg Trp Thr     #               255     - Ala Val Gln Cys Leu Glu Ser Asp Tyr Phe Ly - #s Glu Leu     #               265     - (2) INFORMATION FOR SEQ ID NO:34:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 264     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:     #melanogaster ORGANISM: Drosophila     #Column 39 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:34:     - Asp Asp Tyr Gln Leu Val Arg Lys Leu Gly Ar - #g Gly Lys Tyr Ser     #               015     - Glu Val Phe Glu Ala Ile Asn Ile Thr Thr Th - #r Glu Lys Cys Val     #               030     - Val Lys Ile Leu Lys Pro Val Lys Lys Lys Ly - #s Ile Lys Arg Glu     #               045     - Ile Lys Ile Leu Glu Asn Leu Arg Gly Gly Th - #r Asn Ile Ile Thr     #               060     - Leu Leu Ala Val Val Lys Asp Pro Val Ser Ar - #g Thr Pro Ala Leu     #               075     - Ile Phe Glu His Val Asn Asn Thr Asp Phe Ly - #s Gln Leu Tyr Gln     #               090     - Thr Leu Thr Asp Tyr Glu Ile Arg Tyr Tyr Le - #u Phe Glu Leu Leu     #               105     - Lys Ala Leu Asp Tyr Cys His Ser Met Gly Il - #e Met His Arg Asp     #               120     - Val Lys Pro His Asn Val Met Ile Asp His Gl - #u Asn Arg Lys Leu     #               135     - Arg Leu Ile Asp Trp Gly Leu Ala Glu Phe Ty - #r His Pro Gly Gln     #               150     - Glu Tyr Asn Val Arg Val Ala Ser Arg Tyr Ph - #e Lys Gly Pro Glu     #               165     - Leu Leu Val Asp Tyr Gln Met Tyr Asp Tyr Se - #r Leu Asp Met Trp     #               180     - Ser Leu Gly Cys Met Leu Ala Ser Met Ile Ph - #e Arg Lys Phe Pro     #               195     - Phe Phe His Gly His Asp Asn Tyr Asp Gln Le - #u Val Arg Ile Ala     #               210     - Lys Val Leu Gly Thr Glu Glu Leu Tyr Ala Ty - #r Leu Asp Lys Tyr     #               225     - Asn Ile Asp Leu Val Ser Pro Glu Ala Leu As - #p Phe Leu Asp Lys     #               240     - Leu Leu Arg Tyr Asp His Val Asp Arg Leu Th - #r Ala Arg Glu Ala     #               255     - Met Ala His Pro Tyr Phe Leu Pro Ile                     260     - (2) INFORMATION FOR SEQ ID NO:35:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 276     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 40 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:35:     - Asn Glu Tyr Lys Leu Ile Asp Lys Ile Gly Gl - #u Gly Thr Phe Ser     #               015     - Ser Val Tyr Lys Ala Lys Asp Ile Thr Gly Ly - #s Ile Thr Lys Lys     #               030     - Phe Ala Ser His Phe Trp Asn Tyr Gly Ser As - #n Tyr Val Ala Leu     #               045     - Lys Lys Ile Tyr Val Thr Ser Ser Pro Gln Ar - #g Ile Tyr Asn Glu     #               060     - Leu Asn Leu Leu Tyr Ile Met Thr Gly Ser Se - #r Arg Val Ala Pro     #               075     - Leu Cys Asp Ala Lys Arg Val Arg Asp Gln Va - #l Ile Ala Val Leu     #               090     - Pro Tyr Tyr Pro His Glu Glu Phe Arg Thr Ph - #e Tyr Arg Asp Leu     #               105     - Pro Ile Lys Gly Ile Lys Lys Tyr Ile Trp Gl - #u Leu Leu Arg Ala     #               120     - Leu Lys Phe Val His Ser Lys Gly Ile Ile Hi - #s Arg Asp Ile Lys     #               135     - Pro Thr Asn Phe Leu Phe Asn Leu Glu Leu Gl - #y Arg Gly Val Leu     #               150     - Val Asp Phe Gly Leu Ala Glu Ala Gln Met As - #p Tyr Lys Ser Met     #               165     - Ile Ser Ala Asn Arg Ala Gly Thr Arg Gly Ph - #e Arg Ala Pro Glu     #               180     - Val Leu Met Lys Cys Gly Ala Gln Ser Thr Ly - #s Ile Asp Ile Trp     #               195     - Ser Val Gly Val Ile Leu Leu Ser Leu Leu Gl - #y Arg Arg Phe Pro     #               210     - Met Phe Gln Ser Leu Asp Asp Ala Asp Ser Le - #u Leu Glu Leu Cys     #               225     - Thr Ile Phe Gly Trp Lys Glu Leu Arg Lys Cy - #s Ala Ala Leu His     #               240     - Gly Asp His Tyr Trp Cys Phe Gln Val Leu Gl - #u Gln Cys Phe Glu     #               255     - Met Asp Pro Gln Lys Arg Ser Ser Ala Glu As - #p Leu Leu Lys Thr     #               270     - Pro Phe Phe Asn Glu Leu                     275     - (2) INFORMATION FOR SEQ ID NO:36:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 270     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 41 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:36:     - Gln Asp Leu Val Gln Leu Gly Lys Ile Gly Al - #a Gly Asn Ser Gly     #               015     - Thr Val Val Lys Ala Leu His Val Pro Asp Se - #r Lys Ile Val Ala     #               030     - Lys Lys Thr Ile Pro Val Glu Gln Asn Asn Se - #r Thr Ile Ile Asn     #               045     - Gln Leu Val Arg Glu Leu Ser Ile Val Lys As - #n Val Lys Pro His     #               060     - Glu Asn Ile Ile Thr Phe Tyr Gly Ala Tyr Ty - #r Asn Gln His Ile     #               075     - Asn Asn Glu Ile Ile Ile Leu Met Glu Tyr Se - #r Asp Cys Gly Ser     #               090     - Leu Asp Lys Ile Leu Ser Val Tyr Lys Arg Ph - #e Val Gln Phe Asn     #               105     - Glu Leu Thr Ile Ser Lys Ile Ala Tyr Gly Va - #l Leu Asn Gly Leu     #               120     - Asp His Leu Tyr Arg Gln Lys Ile Ile His Ar - #g Asp Ile Lys Pro     #               135     - Ser Asn Val Leu Ile Asn Ser Lys Gly Gln Il - #e Lys Leu Cys Asp     #               150     - Phe Gly Val Ser Lys Lys Leu Ile Asn Ser Il - #e Ala Asp Thr Phe     #               165     - Val Gly Thr Ser Thr Tyr Met Ser Pro Glu Ar - #g Ile Gln Gly Asn     #               180     - Val Tyr Ser Ile Lys Gly Asp Val Trp Ser Le - #u Gly Leu Met Ile     #               195     - Ile Glu Leu Val Thr Gly Glu Phe Pro Leu Gl - #y Gly His Asn Asp     #               210     - Thr Pro Asp Gly Ile Leu Asp Leu Leu Gln Ar - #g Ile Val Asn Glu     #               225     - Pro Ser Pro Arg Leu Pro Lys Asp Arg Ile Ty - #r Ser Lys Glu Met     #               240     - Thr Asp Phe Val Asn Arg Cys Cys Ile Lys As - #n Glu Arg Glu Arg     #               255     - Ser Ser Ile His Glu Leu Leu His His Asp Le - #u Ile Met Lys Tyr     #               270     - (2) INFORMATION FOR SEQ ID NO:37:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 267     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: mouse     #Column 42 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:37:     - Asp Glu Leu Glu Phe Leu Asp Glu Leu Gly Hi - #s Gly Asn Tyr Gly     #               015     - Asn Val Ser Lys Val Leu His Lys Pro Thr As - #n Val Ile Met Ala     #               030     - Thr Lys Glu Val Arg Leu Glu Leu Asp Glu Al - #a Lys Phe Arg Gln     #               045     - Ile Leu Met Glu Leu Glu Val Leu His Lys Cy - #s Asn Ser Pro Tyr     #               060     - Ile Val Asp Phe Tyr Gly Ala Phe Phe Ile Gl - #u Gly Ala Val Tyr     #               075     - Met Cys Met Glu Tyr Met Asp Gly Gly Ser Le - #u Asp Lys Ile Tyr     #               090     - Asp Glu Ser Ser Glu Ile Gly Gly Ile Asp Gl - #u Pro Gln Leu Ala     #               105     - Phe Ile Ala Asn Ala Val Ile His Gly Leu Ly - #s Glu Leu Lys Glu     #               120     - Gln Asn Ile Ile His Arg Asp Val Lys Pro Th - #r Asn Ile Leu Cys     #               135     - Ser Ala Asn Gln Gly Thr Val Lys Leu Cys As - #p Phe Gly Val Ser     #               150     - Gly Asn Leu Val Ala Ser Leu Ala Lys Thr As - #n Ile Gly Cys Gln     #               165     - Ser Tyr Met Ala Pro Glu Arg Ile Lys Ser Le - #u Asn Asp Arg Ala     #               180     - Thr Tyr Thr Val Gln Ser Asp Ile Trp Ser Le - #u Gly Leu Ser Ile     #               195     - Leu Glu Met Ala Leu Gly Arg Tyr Pro Tyr Pr - #o Pro Glu Thr Tyr     #               210     - Asp Asn Ile Phe Ser Gln Leu Ser Ala Ile Va - #l Asp Gly Pro Pro     #               225     - Pro Arg Leu Pro Ser Asp Lys Phe Ser Ser As - #p Ala Gln Asp Phe     #               240     - Val Ser Leu Cys Leu Gln Lys Ile Pro Glu Ar - #g Arg Pro Thr Tyr     #               255     - Ala Ala Leu Thr Glu His Pro Trp Leu Val Ly - #s Tyr     #               265     - (2) INFORMATION FOR SEQ ID NO:38:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 269     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 43 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:38:     - Thr Arg Phe Arg Asn Val Thr Leu Leu Gly Se - #r Gly Glu Phe Ser     #               015     - Glu Val Phe Gln Val Glu Asp Pro Val Glu Ly - #s Thr Leu Lys Tyr     #               030     - Ala Val Lys Lys Leu Lys Val Lys Phe Ser Gl - #y Pro Lys Glu Arg     #               045     - Asn Arg Leu Leu Gln Glu Val Ser Ile Gln Ar - #g Ala Leu Lys Gly     #               060     - His Asp His Ile Val Glu Leu Met Asp Ser Tr - #p Glu His Gly Gly     #               075     - Phe Leu Tyr Met Gln Val Glu Leu Cys Glu As - #n Gly Ser Leu Asp     #               090     - Arg Phe Leu Glu Glu Gln Gly Gln Leu Ser Ar - #g Leu Asp Glu Phe     #               105     - Arg Val Trp Lys Ile Leu Val Glu Val Ala Le - #u Gly Leu Gln Phe     #               120     - Ile His His Lys Asn Tyr Val His Leu Asp Le - #u Lys Pro Ala Asn     #               135     - Val Met Ile Thr Phe Glu Gly Thr Leu Lys Il - #e Gly Asp Phe Gly     #               150     - Met Ala Ser Val Trp Pro Val Pro Arg Gly Me - #t Glu Arg Glu Gly     #               165     - Asp Cys Glu Tyr Ile Ala Pro Glu Val Leu Al - #a Asn His Leu Tyr     #               180     - Asp Lys Pro Ala Asp Ile Trp Ser Leu Gly Il - #e Thr Val Phe Glu     #               195     - Ala Ala Ala Asn Ile Val Leu Pro Asp Asn Gl - #y Gln Ser Trp Gln     #               210     - Lys Leu Arg Ser Gly Asp Leu Ser Asp Ala Pr - #o Arg Leu Ser Ser     #               225     - Thr Asp Asn Gly Ser Ser Leu Gly Gln Gly Gl - #y Leu Asp Arg Val     #               240     - Val Glu Trp Met Leu Ser Pro Glu Pro Arg As - #n Arg Pro Thr Ile     #               255     - Asp Gln Ile Leu Ala Thr Asp Glu Val Cys Tr - #p Val Glu Met     #               265     - (2) INFORMATION FOR SEQ ID NO:39:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 266     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 44 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:39:     - Met Gly Phe Thr Ile His Gly Ala Leu Thr Pr - #o Gly Ser Glu Gly     #               015     - Cys Val Phe Asp Ser Ser His Pro Asp Tyr Pr - #o Gln Arg Val Ile     #               030     - Val Lys Ala Gly Trp Tyr Thr Ser Thr Ser Hi - #s Glu Ala Arg Leu     #               045     - Leu Arg Arg Leu Asp His Pro Ala Ile Leu Pr - #o Leu Leu Asp Leu     #               060     - His Val Val Ser Gly Val Thr Cys Leu Val Le - #u Pro Lys Tyr Gln     #               075     - Ala Asp Leu Tyr Thr Tyr Leu Ser Arg Arg Le - #u Asn Pro Leu Gly     #               090     - Arg Pro Gln Ile Ala Ala Val Ser Arg Gln Le - #u Leu Ser Ala Val     #               105     - Asp Tyr Ile His Arg Gln Gly Ile Ile His Ar - #g Asp Ile Lys Thr     #               120     - Glu Asn Ile Phe Ile Asn Thr Pro Glu Asp Il - #e Cys Leu Gly Asp     #               135     - Phe Gly Ala Ala Cys Phe Val Gln Gly Ser Ar - #g Ser Ser Pro Phe     #               150     - Tyr Gly Ile Ala Gly Thr Ile Asp Thr Asn Al - #a Pro Glu Val Leu     #               165     - Ala Gly Asp Pro Tyr Thr Thr Thr Val Asp Il - #e Trp Ser Ala Gly     #               180     - Leu Val Ile Phe Glu Thr Ala Val His Asn Al - #a Ser Leu Phe Ser     #               195     - Ala Pro Arg Gly Pro Lys Arg Gly Pro Cys Se - #r Gln Ile Thr Arg     #               210     - Ile Ile Arg Gln Ala Gln Val His Val Asp Gl - #u Phe Ser Pro His     #               225     - Pro Glu Ser Arg Leu Thr Met Asp Ile Asp Va - #l Glu Tyr Leu Val     #               240     - Cys Lys Ala Leu Thr Phe Asp Gly Ala Leu Ar - #g Pro Ser Ala Ala     #               255     - Glu Leu Leu Cys Leu Pro Leu Phe Gln Gln Ly - #s     #               265     - (2) INFORMATION FOR SEQ ID NO:40:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 266     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: mouse     #Column 45 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:40:     - Gly Ser Leu Arg Phe Val Ser Ile Ile Gly Al - #a Gly Ala Tyr Gly     #               015     - Val Val Tyr Lys Ala Glu Asp Ile Tyr Asp Gl - #y Thr Leu Tyr Ala     #               030     - Val Lys Ala Leu Cys Lys Asp Gly Leu Asn Gl - #u Lys Gln Lys Lys     #               045     - Leu Gln Ala Arg Glu Leu Ala Leu His Ala Ar - #g Val Ser Ser His     #               060     - Pro Tyr Ile Ile Thr Leu His Arg Val Leu Gl - #u Thr Glu Asp Ala     #               075     - Ile Tyr Val Val Leu Gln Tyr Cys Pro Asn Gl - #y Asp Leu Phe Thr     #               090     - Tyr Ile Thr Glu Lys Lys Val Tyr Gln Gly As - #n Ser His Leu Ile     #               105     - Lys Thr Val Phe Leu Gln Leu Ile Ser Ala Va - #l Glu His Cys His     #               120     - Ser Val Gly Ile Tyr His Arg Asp Leu Lys Pr - #o Glu Asn Ile Met     #               135     - Val Gly Asn Asp Gly Asn Thr Val Tyr Leu Al - #a Asp Phe Gly Leu     #               150     - Ala Thr Thr Glu Pro Tyr Ser Ser Asp Phe Gl - #y Cys Gly Ser Leu     #               165     - Phe Tyr Met Ser Pro Glu Cys Gln Arg Glu Va - #l Lys Ser Ser Ser     #               180     - Phe Ala Thr Ala Pro Asn Asp Val Trp Ala Le - #u Gly Ile Ile Leu     #               195     - Ile Asn Leu Cys Cys Lys Arg Asn Pro Trp Ly - #s Arg Ala Cys Ser     #               210     - Gln Thr Asp Gly Thr Tyr Arg Ser Tyr Val Hi - #s Asn Pro Ser Thr     #               225     - Leu Leu Ser Ile Leu Pro Ile Ser Arg Glu Le - #u Asn Ser Leu Leu     #               240     - Asn Arg Ile Phe Asp Arg Asn Pro Lys Thr Ar - #g Ile Thr Leu Pro     #               255     - Glu Leu Ser Thr Leu Val Ser Asn Cys Lys As - #n     #               265     - (2) INFORMATION FOR SEQ ID NO:41:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 257     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: rat     #Column 46 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:41:     - Ser Gln Tyr Gln Val Gly Pro Leu Leu Gly Se - #r Gly Gly Phe Gly     #               015     - Ser Val Tyr Ser Gly Ile Arg Val Ala Asp As - #n Leu Pro Val Ala     #               030     - Ile Lys His Val Glu Lys Asp Arg Ile Ser As - #p Trp Gly Glu Asn     #               045     - Gly Thr Arg Val Pro Met Glu Val Val Leu Le - #u Lys Lys Val Ser     #               060     - Ser Asp Phe Ser Gly Val Ile Arg Leu Leu As - #p Trp Phe Glu Arg     #               075     - Pro Asp Ser Phe Val Leu Ile Leu Glu Arg Pr - #o Glu Pro Val Gln     #               090     - Asp Leu Phe Asp Phe Ile Thr Glu Arg Gly Al - #a Leu Gln Glu Asp     #               105     - Leu Ala Arg Gly Phe Phe Trp Gln Val Leu Gl - #u Ala Val Arg His     #               120     - Cys His Asn Cys Gly Val Leu His Arg Asp Il - #e Lys Asp Glu Asn     #               135     - Ile Leu Ile Asp Leu Ser Arg Gly Glu Ile Ly - #s Leu Ile Asp Phe     #               150     - Gly Ser Gly Ala Leu Leu Lys Asp Thr Val Ty - #r Thr Asp Phe Asp     #               165     - Gly Thr Arg Val Tyr Ser Pro Pro Glu Trp Il - #e Arg Tyr His Arg     #               180     - Tyr His Gly Arg Ser Ala Ala Val Trp Ser Le - #u Gly Ile Leu Leu     #               195     - Tyr Asp Met Val Cys Gly Asp Ile Pro Phe As - #p Glu His Asp Glu     #               210     - Glu Ile Ile Lys Gly Gln Val Phe Phe Arg Gl - #n Thr Val Ser Ser     #               225     - Glu Cys Gln His Leu Ile Lys Trp Cys Leu Se - #r Leu Arg Pro Ser     #               240     - Asp Arg Pro Ser Phe Glu Glu Ile Arg Asn Hi - #s Pro Trp Met Gln     #               255     - Gly Asp     - (2) INFORMATION FOR SEQ ID NO:42:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 267     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Saccharomyce - #s cerevisiae     #Column 48 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:42:     - Ser Glu Val Met Leu Ser Thr Arg Ile Gly Se - #r Gly Ser Phe Gly     #               015     - Thr Val Tyr Lys Gly Lys Trp His Gly Asp Va - #l Ala Val Lys Ile     #               030     - Leu Lys Val Val Asp Pro Thr Pro Glu Leu Gl - #n Phe Gln Ala Phe     #               045     - Arg Asn Glu Val Ala Val Leu Arg Lys Thr Ar - #g His Val Asn Ile     #               060     - Leu Leu Phe Met Gly Tyr Met Thr Lys Asp As - #n Leu Ala Ile Val     #               075     - Thr Gln Trp Cys Glu Gly Ser Ser Leu Tyr Ly - #s His Leu His Val     #               090     - Gln Glu Thr Lys Phe Gln Met Phe Gln Leu Il - #e Asp Ile Ala Arg     #               105     - Gln Thr Ala Gln Gly Met Asp Tyr Leu His Al - #a Lys Asn Ile Ile     #               120     - His Arg Asp Met Lys Ser Asn Asn Ile Phe Le - #u His Glu Gly Leu     #               135     - Thr Val Lys Ile Gly Asp Phe Gly Leu Ala Th - #r Val Lys Ser Arg     #               150     - Trp Ser Gly Ser Gln Gln Glu Gln Pro Thr Gl - #y Ser Val Leu Trp     #               165     - Met Ala Pro Glu Val Ile Arg Met Gln Asp As - #n Asn Pro Phe Ser     #               180     - Phe Gln Ser Asp Val Tyr Ser Tyr Gly Ile Va - #l Leu Tyr Glu Leu     #               195     - Met Thr Gly Glu Leu Pro Tyr Ser His Ile As - #n Asn Arg Asp Gln     #               210     - Ile Ile Phe Met Val Gly Arg Gly Tyr Ala Se - #r Pro Asp Leu Ser     #               225     - Lys Leu Tyr Lys Asn Cys Pro Lys Ala Met Ly - #s Arg Leu Val Ala     #               240     - Asp Cys Val Lys Lys Val Lys Glu Glu Arg Pr - #o Leu Phe Pro Gln     #               255     - Ile Leu Ser Ser Ile Glu Leu Leu Gln His Se - #r Leu     #               265     - (2) INFORMATION FOR SEQ ID NO:43:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 266     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Saccharomyce - #s cerevisiae     #Column 49 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:43:     - Ser Glu Val Gln Leu Leu Lys Arg Ile Gly Th - #r Gly Ser Phe Gly     #               015     - Thr Val Phe Arg Gly Arg Trp His Gly Asp Va - #l Ala Val Lys Val     #               030     - Leu Lys Val Ser Gln Pro Thr Ala Glu Gln Al - #a Gln Ala Phe Lys     #               045     - Asn Glu Met Gln Val Leu Arg Lys Thr Arg Hi - #s Val Asn Ile Leu     #               060     - Leu Phe Met Gly Phe Met Thr Arg Pro Gly Ph - #e Ala Ile Ile Thr     #               075     - Gln Trp Cys Glu Gly Ser Ser Leu Tyr His Hi - #s Leu His Val Ala     #               090     - Asp Thr Arg Phe Asp Met Val Gln Leu Ile As - #p Val Ala Arg Gln     #               105     - Thr Ala Gln Gly Met Asp Tyr Leu His Ala Ly - #s Asn Ile Ile His     #               120     - Arg Asp Leu Lys Ser Asn Asn Ile Phe Leu Hi - #s Glu Gly Leu Thr     #               135     - Val Lys Ile Gly Asp Phe Gly Leu Ala Thr Va - #l Lys Thr Arg Trp     #               150     - Ser Gly Ala Gln Pro Glu Gln Pro Ser Gly Se - #r Val Leu Trp Met     #               165     - Ala Ala Glu Val Ile Arg Met Gln Asp Pro As - #n Pro Tyr Ser Phe     #               180     - Gln Ser Asp Val Tyr Ala Tyr Gly Val Val Le - #u Tyr Glu Leu Met     #               195     - Thr Gly Ser Leu Pro Tyr Ser His Ile Gly Cy - #s Arg Asp Gln Ile     #               210     - Ile Phe Met Val Gly Arg Gly Tyr Leu Ser Pr - #o Asp Leu Ser Lys     #               225     - Ile Ser Ser Asn Cys Pro Lys Ala Met Arg Ar - #g Leu Leu Ser Asp     #               240     - Cys Leu Lys Phe Gln Arg Glu Glu Arg Pro Le - #u Phe Pro Gln Ile     #               255     - Leu Ala Thr Ile Glu Leu Leu Gln Arg Ser Le - #u     #               265     - (2) INFORMATION FOR SEQ ID NO:44:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 266     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Saccharomyce - #s cerevisiae     #Column 50 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:44:     - Ser Glu Val Gln Leu Leu Lys Arg Ile Gly Th - #r Gly Ser Phe Gly     #               015     - Thr Val Phe Arg Gly Arg Trp His Gly Asp Va - #l Ala Val Lys Val     #               030     - Leu Lys Val Ser Gln Pro Thr Ala Glu Gln Al - #a Gln Ala Phe Lys     #               045     - Asn Glu Met Gln Val Leu Arg Lys Thr Arg Hi - #s Val Asn Ile Leu     #               060     - Pro Phe Met Gly Phe Met Thr Arg Pro Gly Va - #l Ala Ile Ile Thr     #               075     - Gln Trp Cys Glu Gly Ser Ser Leu Tyr His Hi - #s Leu His Val Ala     #               090     - Asp Thr Arg Phe Asp Met Val Gln Leu Ile As - #p Val Ala Arg Gln     #               105     - Thr Ala Gln Gly Met Asp Tyr Leu His Ala Ly - #s Asn Ile Ile His     #               120     - Arg Asp Leu Lys Ser Asn Asn Ile Phe Leu Hi - #s Glu Gly Leu Thr     #               135     - Val Lys Ile Gly Asp Phe Gly Leu Ala Thr Va - #l Lys Thr Arg Trp     #               150     - Ser Gly Ala Gln Pro Glu Gln Pro Ser Gly Pr - #o Val Leu Trp Met     #               165     - Ala Ala Glu Val Thr Arg Met Gln Asp Pro As - #n Pro Tyr Ser Phe     #               180     - Gln Ser Asp Val Tyr Ala Tyr Gly Val Val Le - #u Tyr Glu Leu Met     #               195     - Thr Gly Ser Leu Pro Tyr Ser His Ile Gly Cy - #s Arg Asp Gln Ile     #               210     - Ile Phe Met Val Gly Arg Gly Tyr Leu Ser Pr - #o Asp Leu Ser Lys     #               225     - Ile Ser Ser Asn Cys Pro Lys Ala Met Arg Ar - #g Leu Leu Ser Asp     #               240     - Cys Leu Lys Phe Gln Arg Glu Glu Arg Pro Le - #u Phe Pro Gln Ile     #               255     - Leu Ala Thr Ile Glu Leu Leu Gln Arg Ser Le - #u     #               265     - (2) INFORMATION FOR SEQ ID NO:45:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 267     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Schizosaccha - #romyces pombe     #Column 51 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:45:     - Ser Glu Val Gln Leu Leu Lys Arg Ile Gly Th - #r Gly Ser Phe Gly     #               015     - Thr Val Phe Arg Gly Leu Trp His Gly Asp Va - #l Ala Val Lys Val     #               030     - Leu Lys Val Ala Gln Pro Thr Ala Glu Gln Al - #a Gln Ala Phe Lys     #               045     - Asn Glu Met Gln Val Leu Arg Lys Thr Arg Hi - #s Val Asn Ile Leu     #               060     - Leu Phe Met Gly Phe Met Thr Arg Pro Gly Ph - #e Ala Ile Ile Thr     #               075     - Gln Trp Cys Glu Gly Ser Ser Leu Tyr His Hi - #s Leu His Val Ala     #               090     - Asp Thr Arg Phe Asp Met Val Gln Leu Ile As - #p Val Ala Arg Gln     #               105     - Thr Ala Gln Gly Met Asp Tyr Leu His Ala Ly - #s Asn Ile Ile His     #               120     - Arg Asp Leu Lys Ser Asn Asn Ile Phe Leu Hi - #s Glu Gly Leu Thr     #               135     - Val Lys Ile Gly Asp Phe Gly Leu Ala Thr Va - #l Lys Thr Arg Trp     #               150     - Ser Gly Ala Gln Pro Glu Gln Pro Ser Gly Se - #r Val Leu Trp Met     #               165     - Ala Ala Glu Val Ile Arg Met Gln Asp Pro As - #n Pro Tyr Ser Phe     #               180     - Gln Ser Asp Val Tyr Ala Tyr Gly Val Val Le - #u Tyr Glu Leu Met     #               195     - Thr Gly Ser Leu Pro Tyr Ser His Ile Gly Se - #r Arg Asp Gln Ile     #               210     - Ile Phe Met Val Gly Arg Gly Tyr Leu Ser Pr - #o Asp Leu Ser Lys     #               225     - Ile Phe Ser Asn Cys Pro Lys Ala Met Arg Ar - #g Leu Leu Thr Asp     #               240     - Cys Leu Lys Phe Gln Arg Glu Glu Arg Pro Le - #u Phe Pro Gln Ile     #               255     - Leu Ala Thr Ile Glu Glu Leu Leu Gln Arg Se - #r Leu     #               265     - (2) INFORMATION FOR SEQ ID NO:46:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 271     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Schizosaccha - #romyces pombe     #Column 52 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:46:     - Glu Gln Val Cys Leu Leu Gln Arg Leu Gly Al - #a Gly Gly Phe Gly     #               015     - Ser Val Tyr Lys Ala Thr Tyr Arg Gly Val Pr - #o Val Ala Ile Lys     #               030     - Gln Val Asn Lys Cys Thr Lys Asn Arg Leu Al - #a Ser Arg Arg Ser     #               045     - Phe Trp Ala Glu Leu Asn Val Ala Arg Leu Ar - #g His Asp Asn Ile     #               060     - Val Arg Val Val Ala Ala Ser Thr Arg Thr Pr - #o Ala Gly Ser Asn     #               075     - Ser Leu Gly Thr Ile Ile Met Glu Phe Gly Gl - #y Asn Val Thr Leu     #               090     - His Gln Val Ile Tyr Gly Ala Ala Gly His Pr - #o Glu Leu Ser Leu     #               105     - Gly Lys Cys Leu Lys Tyr Ser Leu Asp Val Va - #l Asn Gly Leu Leu     #               120     - Phe Leu His Ser Gln Ser Ile Val His Leu As - #p Leu Lys Pro Ala     #               135     - Asn Ile Leu Ile Ser Glu Gln Asp Val Cys Ly - #s Ile Ser Asp Phe     #               150     - Gly Cys Ser Glu Lys Leu Glu Asp Leu Leu Cy - #s Phe Gln Thr Tyr     #               165     - Pro Leu Gly Gly Thr Tyr Thr His Arg Ala Pr - #o Glu Leu Leu Lys     #               180     - Gly Glu Gly Val Thr Pro Lys Ala Asp Ile Ty - #r Ser Phe Ala Ile     #               195     - Thr Leu Trp Gln Met Thr Thr Lys Gln Ala Pr - #o Tyr Ser Gly Glu     #               210     - Arg Gln His Ile Leu Tyr Ala Val Val Ala Ty - #r Asp Leu Arg Pro     #               225     - Ser Leu Ser Ala Ala Val Phe Glu Asp Pro Gl - #y Gln Arg Leu Gly     #               240     - Asp Val Ile Gln Arg Cys Trp Arg Pro Ser Al - #a Ala Gln Arg Pro     #               255     - Ser Ala Arg Leu Leu Leu Val Asp Leu Thr Se - #r Leu Lys Ala Glu     #               270     - Leu     - (2) INFORMATION FOR SEQ ID NO:47:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 271     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: mouse     #Column 53 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:47:     - Glu Gln Val Cys Leu Met His Arg Leu Gly Se - #r Gly Gly Phe Gly     #               015     - Ser Val Tyr Lys Ala Thr Tyr His Gly Val Pr - #o Val Ala Ile Lys     #               030     - Gln Val Asn Lys Cys Thr Lys Asp Leu Arg Al - #a Ser Gln Arg Ser     #               045     - Phe Trp Ala Glu Leu Asn Ile Ala Arg Leu Ar - #g His Asp Asn Ile     #               060     - Val Arg Val Val Ala Ala Ser Thr Arg Thr Pr - #o Glu Asp Ser Asn     #               075     - Ser Leu Gly Thr Ile Ile Met Glu Phe Gly Gl - #y Asn Val Thr Leu     #               090     - His Gln Val Ile Tyr Asp Ala Thr Arg Ser Pr - #o Glu Leu Ser Leu     #               105     - Gly Lys Cys Leu Lys Tyr Ser Leu Asp Val Va - #l Asn Gly Leu Leu     #               120     - Phe Leu His Ser Gln Ser Ile Leu His Leu As - #p Leu Lys Pro Ala     #               135     - Asn Ile Leu Ile Ser Glu Gln Asp Val Cys Ly - #s Ile Ser Asp Phe     #               150     - Gly Cys Ser Gln Lys Leu Gln Asp Leu Arg Gl - #y Arg Gln Ala Pro     #               165     - His Ile Gly Gly Thr Tyr Thr His Gln Ala Pr - #o Glu Ile Leu Lys     #               180     - Gly Glu Ile Ala Thr Pro Lys Ala Asp Ile Ty - #r Ser Phe Gly Ile     #               195     - Thr Leu Trp Gln Met Thr Thr Arg Glu Val Pr - #o Tyr Ser Gly Glu     #               210     - Pro Gln Tyr Val Gln Tyr Ala Val Val Ala Ty - #r Asn Leu Arg Pro     #               225     - Ser Leu Ala Gly Ala Val Phe Thr Ala Thr Gl - #y Lys Thr Leu Gln     #               240     - Asn Ile Ile Gln Ser Cys Trp Glu Ala Arg Al - #a Leu Gln Arg Pro     #               255     - Gly Ala Glu Leu Leu Gln Arg Asp Leu Lys Al - #a Phe Arg Gly Ala     #               270     - Leu     - (2) INFORMATION FOR SEQ ID NO:48:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 269     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Herpes si - #mplex     #Column 54 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:48:     - Gly Gln Val Cys Leu Leu His Arg Leu Gly Se - #r Gly Gly Phe Gly     #               015     - Ser Val Tyr Lys Ala Thr Tyr His Gly Val Pr - #o Val Ala Ile Lys     #               030     - Gln Val Asn Lys Cys Thr Arg Thr Leu Arg Al - #a Ser Gln Arg Asn     #               045     - Phe Trp Ala Glu Leu Asn Ile Ala Arg Leu Hi - #s His Asp Asn Ile     #               060     - Ile Arg Val Val Ala Ala Ser Thr Arg Thr Pr - #o Glu Gly Ser Asn     #               075     - Ser Leu Gly Thr Ile Ile Met Glu Phe Gly Gl - #y Asn Val Thr Leu     #               090     - His Gln Val Ile Tyr Gly Ala Thr Arg Ser Pr - #o Glu Leu Ser Leu     #               105     - Gly Lys Cys Leu Lys Tyr Ser Leu Asp Ile Va - #l Asn Gly Leu Leu     #               120     - Phe Leu His Ser Gln Ser Ile Leu His Leu As - #p Leu Lys Pro Ala     #               135     - Asn Ile Leu Ile Ser Glu Lys Asp Val Cys Ly - #s Ile Ser Asp Phe     #               150     - Gly Cys Ser Gln Lys Leu Gln Asp Leu Arg Cy - #s Arg Pro His His     #               165     - Ile Gly Gly Thr Tyr Thr His Gln Ala Pro Gl - #u Leu Leu Lys Gly     #               180     - Glu Ile Ala Thr Pro Lys Ala Asp Ile Tyr Se - #r Phe Gly Ile Thr     #               195     - Leu Trp Gln Met Thr Thr Arg Glu Val Pro Ty - #r Ser Gly Glu Pro     #               210     - Gln His Val Gln Tyr Ala Val Val Ala Tyr As - #n Leu Arg Pro His     #               225     - Trp Gln Ala Val Phe Thr Ala Thr Gly Lys Th - #r Leu Gln Asn Asn     #               240     - Val Gln Ser Cys Trp Glu Ala Arg Ala Leu Gl - #n Arg Pro Gly Ala     #               255     - Glu Leu Leu Gln Lys Asp Leu Lys Ala Phe Ar - #g Gly Ala Leu     #               265     - (2) INFORMATION FOR SEQ ID NO:49:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 259     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 56 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:49:     - Glu Ser Leu Arg Leu Glu Val Lys Leu Gly Gl - #n Gly Cys Phe Gly     #               015     - Glu Val Trp Met Gly Thr Trp Asn Gly Thr Th - #r Arg Val Ala Ile     #               030     - Lys Thr Leu Lys Pro Gly Thr Met Ser Pro Gl - #u Ala Phe Leu Gln     #               045     - Glu Ala Gln Val Met Lys Lys Leu Arg His Gl - #u Lys Leu Val Gln     #               060     - Leu Tyr Ala Val Val Ser Glu Glu Pro Ile Ty - #r Ile Val Thr Glu     #               075     - Tyr Met Ser Lys Gly Ser Leu Leu Asp Phe Le - #u Lys Gly Glu Thr     #               090     - Gly Lys Tyr Leu Arg Leu Pro Gln Leu Val As - #p Met Ala Ala Gln     #               105     - Ile Ala Ser Gly Met Ala Tyr Val Glu Arg Me - #t Asn Tyr Val His     #               120     - Arg Asp Leu Arg Ala Ala Asn Ile Leu Val Gl - #y Glu Asn Leu Val     #               135     - Cys Lys Val Ala Asp Phe Gly Leu Ala Arg Le - #u Ile Glu Asp Asn     #               150     - Glu Tyr Thr Ala Arg Gln Gly Ala Lys Phe Pr - #o Ile Lys Trp Thr     #               165     - Ala Pro Glu Ala Ala Leu Tyr Gly Arg Phe Th - #r Ile Lys Ser Asp     #               180     - Val Trp Ser Phe Gly Ile Leu Leu Thr Glu Le - #u Thr Thr Lys Gly     #               195     - Arg Val Pro Tyr Pro Gly Met Val Asn Arg Gl - #u Val Leu Asp Gln     #               210     - Val Glu Arg Gly Tyr Arg Met Pro Cys Pro Pr - #o Glu Cys Pro Glu     #               225     - Ser Leu His Asp Leu Met Cys Gln Cys Trp Ar - #g Lys Glu Pro Glu     #               240     - Glu Arg Pro Thr Phe Glu Tyr Leu Gln Ala Ph - #e Leu Glu Asp Tyr     #               255     - Phe Thr Ser Thr     - (2) INFORMATION FOR SEQ ID NO:50:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 259     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 57 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:50:     - Glu Ser Leu Arg Leu Glu Val Lys Leu Gly Gl - #n Gly Cys Phe Gly     #               015     - Glu Val Trp Met Gly Thr Trp Asn Gly Thr Th - #r Lys Val Ala Ile     #               030     - Lys Thr Leu Lys Pro Gly Thr Met Met Pro Gl - #u Ala Phe Leu Gln     #               045     - Glu Ala Gln Ile Met Lys Lys Leu Arg His As - #p Lys Leu Val Pro     #               060     - Leu Tyr Ala Val Val Ser Glu Glu Pro Ile Ty - #r Ile Val Thr Glu     #               075     - Phe Met Ser Lys Gly Ser Leu Leu Asp Phe Le - #u Lys Glu Gly Asp     #               090     - Gly Lys Tyr Leu Lys Leu Pro Gln Leu Val As - #p Met Ala Ala Gln     #               105     - Ile Ala Asp Gly Met Ala Tyr Ile Glu Arg Me - #t Asn Tyr Ile His     #               120     - Arg Asp Leu Arg Ala Ala Asn Ile Leu Val Gl - #y Glu Asn Leu Val     #               135     - Cys Lys Ile Ala Asp Phe Gly Leu Ala Arg Le - #u Ile Glu Asp Asn     #               150     - Glu Tyr Thr Ala Arg Gln Gly Ala Lys Phe Pr - #o Ile Lys Trp Thr     #               165     - Ala Pro Glu Ala Ala Leu Tyr Gly Arg Phe Th - #r Ile Lys Ser Asp     #               180     - Val Trp Ser Phe Gly Ile Leu Gln Thr Glu Le - #u Val Thr Lys Gly     #               195     - Arg Val Pro Tyr Pro Gly Met Val Asn Arg Gl - #u Val Leu Glu Gln     #               210     - Val Glu Arg Gly Tyr Arg Met Pro Cys Pro Gl - #n Gly Cys Pro Glu     #               225     - Ser Leu His Glu Leu Met Asn Leu Cys Trp Ly - #s Lys Asp Pro Asp     #               240     - Glu Arg Pro Thr Phe Glu Tyr Ile Gln Ser Ph - #e Leu Glu Asp Tyr     #               255     - Phe Thr Ala Thr     - (2) INFORMATION FOR SEQ ID NO:51:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 259     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 58 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:51:     - Ser Ser Ile Thr Leu Glu Arg Arg Leu Gly Th - #r Gly Cys Phe Gly     #               015     - Asp Val Trp Leu Gly Thr Trp Asn Gly Ser Th - #r Lys Val Ala Val     #               030     - Lys Thr Leu Lys Pro Gly Thr Met Ser Pro Ly - #s Ala Phe Leu Glu     #               045     - Glu Ala Gln Val Met Lys Leu Leu Arg His As - #p Lys Leu Val Gln     #               060     - Leu Tyr Ala Val Val Ser Glu Glu Pro Ile Ty - #r Ile Val Thr Glu     #               075     - Phe Met Cys His Gly Ser Leu Leu Asp Phe Le - #u Lys Asn Pro Glu     #               090     - Gly Gln Asp Leu Arg Leu Pro Gln Leu Val As - #p Met Ala Ala Gln     #               105     - Val Ala Glu Gly Met Ala Tyr Met Glu Arg Me - #t Asn Tyr Ile His     #               120     - Arg Asp Leu Arg Ala Ala Asn Ile Leu Val Gl - #y Glu Arg Leu Ala     #               135     - Cys Lys Ile Ala Asp Phe Gly Leu Ala Arg Le - #u Ile Lys Asp Asp     #               150     - Glu Tyr Asn Pro Cys Gln Gly Ser Lys Phe Pr - #o Ile Lys Trp Thr     #               165     - Ala Pro Glu Ala Ala Leu Phe Gly Arg Phe Th - #r Ile Lys Ser Asp     #               180     - Val Trp Ser Phe Gly Ile Leu Leu Thr Glu Le - #u Ile Thr Lys Gly     #               195     - Arg Ile Pro Tyr Pro Gly Met Asn Lys Arg Gl - #u Val Leu Glu Gln     #               210     - Val Glu Gln Gly Tyr His Met Pro Cys Pro Pr - #o Gly Cys Pro Ala     #               225     - Ser Leu Tyr Glu Ala Met Glu Gln Thr Trp Ar - #g Leu Asp Pro Glu     #               240     - Glu Arg Pro Thr Phe Glu Tyr Leu Gln Ser Ph - #e Leu Glu Asp Tyr     #               255     - Phe Thr Ser Ala     - (2) INFORMATION FOR SEQ ID NO:52:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 259     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 59 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:52:     - Glu Ser Leu Gln Leu Ile Lys Arg Leu Gly As - #n Gly Gln Phe Gly     #               015     - Glu Val Trp Met Gly Thr Trp Asn Gly Asn Th - #r Lys Val Ala Ile     #               030     - Lys Thr Leu Lys Pro Gly Thr Met Ser Pro Gl - #u Ser Phe Leu Glu     #               045     - Glu Ala Gln Ile Met Lys Lys Leu Lys His As - #p Lys Leu Val Gln     #               060     - Leu Tyr Ala Val Val Ser Glu Glu Pro Ile Ty - #r Ile Val Thr Glu     #               075     - Tyr Met Asn Lys Gly Ser Leu Leu Asp Phe Le - #u Lys Asp Gly Glu     #               090     - Gly Arg Ala Leu Lys Leu Pro Asn Leu Val As - #p Met Ala Ala Gln     #               105     - Val Ala Ala Gly Met Ala Tyr Ile Glu Arg Me - #t Asn Tyr Ile His     #               120     - Arg Asp Leu Arg Ser Ala Asn Ile Leu Val Gl - #y Asn Gly Leu Ile     #               135     - Cys Lys Ile Ala Asp Phe Gly Leu Ala Arg Le - #u Ile Glu Asp Asn     #               150     - Glu Tyr Thr Ala Arg Gln Gly Ala Lys Phe Pr - #o Ile Lys Trp Thr     #               165     - Ala Pro Glu Ala Ala Leu Tyr Gly Arg Phe Th - #r Ile Lys Ser Asp     #               180     - Val Trp Ser Phe Gly Ile Leu Leu Thr Glu Le - #u Val Thr Lys Gly     #               195     - Arg Val Pro Tyr Pro Gly Met Asn Asn Arg Gl - #u Val Leu Glu Gln     #               210     - Val Glu Arg Gly Tyr Arg Met Pro Cys Pro Gl - #n Asp Cys Pro Ile     #               225     - Ser Leu His Glu Leu Met Ile His Cys Trp Ly - #s Lys Asp Pro Glu     #               240     - Glu Arg Pro Thr Phe Glu Tyr Leu Gln Ser Ph - #e Leu Glu Asp Tyr     #               255     - Phe Thr Ala Thr     - (2) INFORMATION FOR SEQ ID NO:53:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 260     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 60 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:53:     - Glu Ser Ile Lys Leu Val Lys Arg Leu Gly Al - #a Gly Gln Phe Gly     #               015     - Glu Val Trp Met Gly Tyr Tyr Asn Asn Ser Th - #r Lys Val Ala Val     #               030     - Lys Thr Leu Lys Pro Gly Thr Met Ser Val Gl - #n Ala Phe Leu Glu     #               045     - Glu Ala Asn Leu Met Lys Thr Leu Gln His As - #p Lys Leu Val Arg     #               060     - Leu Tyr Ala Val Val Thr Arg Glu Glu Pro Il - #e Tyr Ile Ile Thr     #               075     - Glu Tyr Met Ala Lys Gly Ser Leu Leu Asp Ph - #e Leu Lys Ser Asp     #               090     - Glu Gly Gly Lys Val Leu Leu Pro Lys Leu Il - #e Asp Phe Ser Ala     #               105     - Gln Ile Ala Glu Gly Met Ala Tyr Ile Glu Ar - #g Lys Asn Tyr Ile     #               120     - His Arg Asp Leu Arg Ala Ala Asn Val Leu Va - #l Ser Glu Ser Leu     #               135     - Met Cys Lys Ile Ala Asp Phe Gly Leu Ala Ar - #g Val Ile Glu Asp     #               150     - Asn Glu Tyr Thr Ala Arg Glu Gly Ala Lys Ph - #e Pro Ile Lys Trp     #               165     - Thr Ala Pro Glu Ala Ile Asn Phe Gly Cys Ph - #e Thr Ile Lys Ser     #               180     - Asp Val Trp Ser Phe Gly Ile Leu Leu Tyr Gl - #u Ile Val Thr Tyr     #               195     - Gly Lys Ile Pro Tyr Pro Gly Arg Thr Asn Al - #a Asp Val Met Thr     #               210     - Ala Leu Ser Gln Gly Tyr Arg Met Pro Arg Va - #l Glu Asn Cys Pro     #               225     - Asp Glu Leu Tyr Asp Ile Met Lys Met Cys Tr - #p Lys Glu Lys Pro     #               240     - Glu Glu Arg Pro Thr Phe Asp Tyr Leu Gln Se - #r Val Leu Asp Asp     #               255     - Phe Tyr Thr Ala Thr                     260     - (2) INFORMATION FOR SEQ ID NO:54:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 259     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 61 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:54:     - Glu Thr Leu Lys Leu Val Glu Arg Leu Gly Al - #a Gly Gln Phe Gly     #               015     - Arg Cys Gly Trp Gly Thr Thr Thr Gly Thr Th - #r Lys Val Ala Val     #               030     - Lys Ser Leu Lys Gln Gly Ser Met Ser Ala Gl - #y Arg Leu Pro Ala     #               045     - Glu Ala Asn Leu Met Lys Gln Leu Gln His Gl - #n Arg Leu Val Arg     #               060     - Leu Tyr Ala Val Val Thr Gln Glu Pro Ile Ty - #r Ile Ile Thr Glu     #               075     - Tyr Met Glu Asn Gly Ser Leu Val Asp Phe Le - #u Lys Thr Pro Ser     #               090     - Gly Ile Lys Leu Thr Ile Asn Lys Leu Leu As - #p Met Ala Ala Gln     #               105     - Ile Ala Glu Gly Met Ala Phe Ile Glu Glu Ar - #g Asn Tyr Ile His     #               120     - Arg Asp Leu Arg Ala Ala Asn Ile Leu Val Se - #r Asp Thr Leu Ser     #               135     - Cys Lys Ile Ala Asp Phe Gly Leu Ala Arg Le - #u Ile Glu Asp Asn     #               150     - Glu Tyr Thr Ala Arg Glu Gly Ala Lys Phe Pr - #o Ile Lys Trp Thr     #               165     - Ala Pro Glu Ala Ile Asn Tyr Gly Thr Phe Th - #r Ile Lys Ser Asp     #               180     - Val Trp Ser Phe Gly Ile Leu Leu Thr Glu Il - #e Val Thr His Gly     #               195     - Arg Ile Pro Tyr Pro Gly Met Thr Asn Pro Gl - #u Val Ile Gln Asn     #               210     - Leu Glu Arg Gly Tyr Arg Met Val Arg Pro As - #p Asn Cys Pro Glu     #               225     - Glu Leu Tyr Gln Leu Met Arg Leu Cys Trp Ly - #s Glu Arg Pro Glu     #               240     - Asp Arg Pro Thr Phe Asp Tyr Leu Arg Ser Va - #l Leu Glu Asp Phe     #               255     - Phe Thr Ala Thr     - (2) INFORMATION FOR SEQ ID NO:55:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 259     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: mouse     #Column 62 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:55:     - Glu Thr Leu Lys Leu Val Glu Arg Leu Gly Al - #a Gly Gln Phe Gly     #               015     - Glu Val Trp Met Gly Tyr Tyr Asn Gly His Th - #r Lys Val Ala Val     #               030     - Lys Ser Leu Lys Gln Gly Ser Met Ser Pro Va - #l Pro Phe Leu Ala     #               045     - Glu Ala Asn Leu Met Lys Gln Leu Gln His Pr - #o Arg Leu Val Arg     #               060     - Leu Tyr Ala Val Val Thr Gln Glu Pro Ile Ty - #r Ile Ile Thr Glu     #               075     - Tyr Met Glu Asn Gly Ser Leu Val Asp Phe Le - #u Lys Thr Pro Ser     #               090     - Gly Ile Lys Leu Asn Val Asn Lys Leu Leu As - #p Met Ala Ala Gln     #               105     - Ile Ala Glu Gly Met Ala Phe Ile Glu Glu Gl - #n Asn Tyr Ile His     #               120     - Arg Asp Leu Arg Ala Ala Asn Ile Leu Val Se - #r Asp Thr Leu Ser     #               135     - Cys Lys Ile Ala Asp Phe Gly Leu Ala Arg Le - #u Ile Glu Asp Asn     #               150     - Glu Tyr Thr Ala Arg Glu Gly Ala Lys Phe Pr - #o Ile Lys Trp Thr     #               165     - Ala Pro Glu Ala Ile Asn Tyr Gly Thr Phe Th - #r Ile Lys Ser Asp     #               180     - Val Trp Ser Phe Gly Ile Leu Leu Thr Glu Il - #e Val Thr His Gly     #               195     - Arg Ile Pro Tyr Pro Gly Met Thr Asn Pro Gl - #u Val Ile Gln Asn     #               210     - Leu Glu Arg Gly Tyr Arg Met Val Arg Pro As - #p Asn Cys Pro Glu     #               225     - Glu Leu Tyr His Leu Met Met Leu Cys Trp Ly - #s Glu Arg Pro Glu     #               240     - Asp Arg Pro Thr Phe Asp Tyr Leu Arg Ser Va - #l Leu Asp Asp Phe     #               255     - Phe Thr Ala Thr     - (2) INFORMATION FOR SEQ ID NO:56:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 259     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 63 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:56:     - Glu Ser Leu Lys Leu Glu Lys Lys Leu Gly Al - #a Gly Gln Phe Gly     #               015     - Glu Val Trp Met Ala Thr Tyr Asn Lys His Th - #r Lys Val Ala Val     #               030     - Lys Thr Met Lys Pro Gly Ser Met Ser Val Gl - #u Ala Phe Leu Ala     #               045     - Glu Ala Asn Val Met Lys Thr Leu Gln His As - #p Lys Leu Val Lys     #               060     - Leu His Ala Val Val Thr Lys Glu Pro Ile Ty - #r Ile Ile Thr Glu     #               075     - Phe Met Ala Lys Gly Ser Leu Leu Asp Phe Le - #u Lys Ser Asp Glu     #               090     - Gly Ser Lys Gln Pro Leu Pro Lys Leu Ile As - #p Phe Ser Ala Gln     #               105     - Ile Ala Glu Gly Met Ala Phe Ile Glu Gln Ar - #g Asn Tyr Ile His     #               120     - Arg Asp Leu Arg Ala Ala Asn Ile Leu Val Se - #r Ala Ser Leu Val     #               135     - Cys Lys Ile Ala Asp Phe Gly Leu Ala Arg Va - #l Ile Glu Asp Asn     #               150     - Glu Tyr Thr Ala Arg Glu Gly Ala Lys Phe Pr - #o Ile Lys Trp Thr     #               165     - Ala Pro Glu Ala Ile Asn Phe Gly Ser Phe Th - #r Ile Lys Ser Asp     #               180     - Val Trp Ser Phe Gly Ile Leu Leu Met Glu Il - #e Val Thr Tyr Gly     #               195     - Arg Ile Pro Tyr Pro Gly Met Ser Asn Pro Gl - #u Val Ile Arg Ala     #               210     - Leu Glu Arg Gly Tyr Arg Met Pro Arg Pro Gl - #u Asn Cys Pro Glu     #               225     - Glu Leu Tyr Asn Ile Met Met Arg Cys Trp Ly - #s Asn Arg Pro Glu     #               240     - Glu Arg Pro Thr Phe Glu Tyr Ile Gln Ser Va - #l Leu Asp Asp Phe     #               255     - Tyr Thr Ala Thr     - (2) INFORMATION FOR SEQ ID NO:57:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 262     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:     #melanogaster ORGANISM: Drosophila     #Column 64 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:57:     - Ser Glu Ile Gln Leu Leu Arg Lys Leu Gly Ar - #g Gly Asn Phe Gly     #               015     - Glu Val Phe Tyr Gly Lys Trp Arg Asn Ser Il - #e Asp Val Ala Val     #               030     - Lys Thr Leu Arg Glu Gly Thr Met Ser Thr Al - #a Ala Phe Leu Gln     #               045     - Glu Ala Ala Ile Met Lys Lys Phe Arg His As - #n Arg Leu Val Ala     #               060     - Leu Tyr Ala Val Cys Ser Gln Glu Glu Pro Il - #e Tyr Ile Val Gln     #               075     - Glu Tyr Met Ser Lys Gly Ser Leu Leu Asp Ph - #e Leu Arg Glu Gly     #               090     - Asp Gly Arg Tyr Leu His Phe Glu Asp Leu Il - #e Tyr Ile Ala Thr     #               105     - Gln Val Ala Ser Gly Met Glu Tyr Leu Glu Se - #r Lys Gln Leu Ile     #               120     - His Arg Asp Leu Ala Ala Arg Asn Val Leu Il - #e Gly Glu Asn Asn     #               135     - Val Ala Lys Ile Cys Asp Phe Gly Leu Ala Ar - #g Val Ile Ala Asp     #               150     - Asp Glu Tyr Cys Pro Lys Gln Gly Ser Arg Ph - #e Pro Val Lys Trp     #               165     - Thr Ala Pro Glu Ala Ile Ile Tyr Gly Lys Ph - #e Ser Ile Lys Ser     #               180     - Asp Val Trp Ser Phe Gly Ile Leu Leu Met Gl - #u Leu Phe Thr Tyr     #               195     - Gly Gln Val Pro Tyr Pro Gly Met His Ser Ar - #g Glu Val Ile Glu     #               210     - Asn Ile Glu Arg Gly Phe Arg Met Pro Lys Pr - #o Thr Asn His Tyr     #               225     - Phe Pro Asp Asn Ile Tyr Gln Leu Leu Leu Gl - #n Cys Trp Asp Ala     #               240     - Val Pro Glu Lys Arg Pro Thr Phe Glu Phe Le - #u Asn His Tyr Phe     #               255     - Glu Ser Phe Ser Thr Thr Ser                     260     - (2) INFORMATION FOR SEQ ID NO:58:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 261     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:     #melanogaster ORGANISM: Drosophila     #Column 65 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:58:     - Ile Gln Leu Met Leu Met Glu Glu Leu Gly Se - #r Gly Gln Phe Gly     #               015     - Val Val Arg Arg Gly Lys Trp Arg Gly Ser Il - #e Asp Thr Ala Val     #               030     - Lys Met Met Lys Glu Gly Thr Met Ser Glu As - #p Asp Phe Ile Glu     #               045     - Glu Ala Lys Val Met Thr Lys Leu Gln His Pr - #o Asn Leu Val Gln     #               060     - Leu Tyr Gly Val Cys Thr Lys His Arg Pro Il - #e Tyr Ile Val Thr     #               075     - Glu Tyr Met Lys His Gly Ser Leu Leu Asn Ph - #e Leu Arg Arg His     #               090     - Glu Lys Thr Leu Ile Gly Asn Met Gly Leu Le - #u Leu Asp Met Cys     #               105     - Ile Gln Val Ser Lys Gly Met Thr Tyr Leu Gl - #u Arg His Asn Tyr     #               120     - Ile His Arg Asp Leu Ala Ala Arg Asn Cys Le - #u Val Gly Ser Glu     #               135     - Asn Val Val Lys Val Ala Asp Phe Gly Leu Al - #a Arg Tyr Val Leu     #               150     - Asp Asp Gln Tyr Thr Ser Ser Gly Gly Thr Ly - #s Phe Pro Ile Lys     #               165     - Trp Ala Pro Pro Glu Val Leu Asn Tyr Thr Ar - #g Phe Ser Ser Lys     #               180     - Ser Asp Val Trp Ala Phe Gly Val Leu Met Tr - #p Glu Ile Phe Thr     #               195     - Cys Gly Lys Met Pro Tyr Gly Arg Leu Lys As - #n Thr Glu Val Val     #               210     - Glu Arg Val Gln Arg Gly Ile Ile Leu Glu Ly - #s Pro Lys Ser Cys     #               225     - Ala Lys Glu Ile Tyr Asp Val Met Lys Leu Cy - #s Trp Ser His Gly     #               240     - Pro Glu Glu Arg Pro Ala Phe Arg Val Leu Me - #t Asp Gln Leu Ala     #               255     - Leu Val Ala Gln Thr Leu                     260     - (2) INFORMATION FOR SEQ ID NO:59:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 261     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 67 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:59:     - Thr Asp Ile Thr Met Lys His Lys Leu Gly Gl - #y Gly Gln Tyr Gly     #               015     - Glu Val Tyr Glu Gly Val Trp Lys Lys Tyr Se - #r Leu Thr Val Ala     #               030     - Val Lys Thr Leu Lys Glu Asp Thr Met Glu Va - #l Glu Glu Phe Leu     #               045     - Lys Glu Ala Ala Val Met Lys Glu Ile Lys Hi - #s Pro Asn Leu Val     #               060     - Gln Leu Leu Gly Val Cys Thr Arg Glu Pro Pr - #o Phe Tyr Ile Ile     #               075     - Thr Glu Phe Met Thr Tyr Gly Asn Leu Leu As - #p Tyr Leu Arg Glu     #               090     - Cys Asn Arg Gln Glu Val Asn Ala Val Val Le - #u Leu Tyr Met Ala     #               105     - Thr Gln Ile Ser Ser Ala Met Glu Tyr Leu Gl - #u Lys Lys Asn Phe     #               120     - Ile His Arg Asp Leu Ala Ala Arg Asn Cys Le - #u Val Gly Glu Asn     #               135     - His Leu Val Lys Val Ala Asp Phe Gly Leu Se - #r Arg Leu Met Thr     #               150     - Gly Asp Thr Tyr Thr Ala His Ala Gly Ala Ly - #s Phe Pro Ile Lys     #               165     - Trp Thr Ala Pro Glu Ser Leu Ala Tyr Asn Ly - #s Phe Ser Ile Lys     #               180     - Ser Asp Val Trp Ala Phe Gly Val Leu Leu Tr - #p Glu Ile Ala Thr     #               195     - Tyr Gly Met Ser Pro Tyr Pro Gly Ile Asp Ar - #g Ser Gln Val Tyr     #               210     - Glu Leu Leu Glu Lys Asp Tyr Arg Met Lys Ar - #g Pro Glu Gly Cys     #               225     - Pro Glu Lys Val Tyr Glu Leu Met Arg Ala Cy - #s Trp Gln Trp Asn     #               240     - Pro Ser Asp Arg Pro Ser Phe Ala Glu Ile Hi - #s Gln Ala Phe Glu     #               255     - Thr Met Phe Gln Glu Ser                     260     - (2) INFORMATION FOR SEQ ID NO:60:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 261     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:     #melanogaster ORGANISM: Drosophila     #Column 68 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:60:     - Thr Asp Ile Met Met Lys His Lys Leu Gly Gl - #y Gly Gln Tyr Gly     #               015     - Glu Val Tyr Glu Ala Val Trp Lys Arg Tyr Gl - #y Asn Thr Val Ala     #               030     - Val Lys Thr Leu Lys Glu Asp Thr Met Ala Le - #u Lys Asp Phe Leu     #               045     - Glu Glu Ala Ala Ile Met Lys Glu Met Lys Hi - #s Pro Asn Leu Val     #               060     - Gln Leu Ile Gly Val Cys Thr Arg Glu Pro Pr - #o Phe Tyr Ile Ile     #               075     - Thr Glu Phe Met Ser His Gly Asn Leu Leu As - #p Phe Leu Arg Ser     #               090     - Ala Gly Arg Glu Thr Leu Asp Ala Val Ala Le - #u Leu Tyr Met Ala     #               105     - Thr Gln Ile Ala Ser Gly Met Ser Tyr Leu Gl - #u Ser Arg Asn Tyr     #               120     - Ile His Arg Asp Leu Ala Ala Arg Asn Cys Le - #u Val Gly Asp Asn     #               135     - Lys Leu Val Lys Val Ala Asp Phe Gly Leu Al - #a Arg Leu Met Arg     #               150     - Asp Asp Thr Tyr Thr Ala His Ala Gly Ala Ly - #s Phe Pro Ile Lys     #               165     - Trp Thr Ala Pro Glu Gly Leu Ala Tyr Asn Ly - #s Phe Ser Thr Lys     #               180     - Ser Asp Val Trp Ala Phe Gly Val Leu Leu Tr - #p Glu Ile Ala Thr     #               195     - Tyr Gly Met Ser Pro Tyr Pro Ala Ile Asp Le - #u Thr Asp Val Tyr     #               210     - His Lys Leu Asp Lys Gly Tyr Arg Met Glu Ar - #g Pro Pro Gly Cys     #               225     - Pro Pro Glu Val Tyr Asp Leu Met Arg Gln Cy - #s Trp Gln Trp Asp     #               240     - Ala Thr Asp Arg Pro Thr Phe Lys Ser Ile Hi - #s His Ala Leu Glu     #               255     - His Met Phe Gln Val Gly                     260     - (2) INFORMATION FOR SEQ ID NO:61:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 261     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Caenorhabdit - #is elegans     #Column 69 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:61:     - Ser Glu Ile Ile Met His Asn Lys Leu Gly Gl - #y Gly Gln Tyr Gly     #               015     - Asp Val Tyr Glu Gly Tyr Trp Lys Arg His As - #p Cys Thr Ile Ala     #               030     - Val Lys Ala Leu Lys Glu Asp Ala Met Pro Le - #u His Glu Phe Leu     #               045     - Ala Glu Ala Ala Ile Met Lys Asp Leu His Hi - #s Lys Asn Leu Val     #               060     - Arg Leu Leu Gly Val Cys Thr His Glu Ala Pr - #o Phe Tyr Ile Ile     #               075     - Thr Glu Phe Met Cys Asn Gly Asn Leu Leu Gl - #u Tyr Leu Arg Arg     #               090     - Thr Asp Lys Ser Leu Leu Pro Pro Ile Ile Le - #u Val Gln Met Ala     #               105     - Ser Gln Ile Ala Ser Gly Met Ser Tyr Leu Gl - #u Ala Arg His Phe     #               120     - Ile His Arg Asp Leu Ala Ala Arg Asn Cys Le - #u Val Ser Glu His     #               135     - Asn Ile Val Lys Ile Ala Asp Phe Gly Leu Al - #a Arg Phe Met Lys     #               150     - Glu Asp Thr Tyr Thr Ala His Ala Gly Ala Ly - #s Phe Pro Ile Lys     #               165     - Trp Thr Ala Pro Glu Gly Leu Ala Phe Asn Th - #r Phe Ser Ser Lys     #               180     - Ser Asp Val Trp Ala Phe Gly Val Leu Leu Tr - #p Glu Ile Ala Thr     #               195     - Tyr Gly Met Ala Pro Tyr Pro Gly Val Glu Le - #u Ser Asn Val Tyr     #               210     - Gly Leu Leu Glu Asn Gly Phe Arg Met Asp Gl - #y Pro Gln Gly Cys     #               225     - Pro Pro Ser Val Tyr Arg Leu Met Leu Gln Cy - #s Trp Asn Trp Ser     #               240     - Pro Ser Asp Arg Pro Arg Phe Arg Asp Ile Hi - #s Phe Asn Leu Glu     #               255     - Asn Leu Ile Ser Ser Asn                     260     - (2) INFORMATION FOR SEQ ID NO:62:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 262     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 70 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:62:     - Glu Asp Leu Val Leu Gly Glu Gln Ile Gly Ar - #g Gly Asn Phe Gly     #               015     - Glu Val Phe Ser Gly Arg Leu Arg Ala Asp As - #n Thr Leu Val Ala     #               030     - Val Lys Ser Cys Arg Glu Thr Leu Pro Pro As - #p Leu Lys Ala Lys     #               045     - Phe Leu Gln Glu Ala Arg Ile Leu Lys Gln Ty - #r Ser His Pro Asn     #               060     - Ile Val Arg Leu Ile Gly Val Cys Thr Gln Ly - #s Gln Pro Ile Tyr     #               075     - Ile Val Met Glu Leu Val Gln Gly Gly Asp Ph - #e Leu Thr Phe Leu     #               090     - Arg Thr Glu Gly Ala Arg Leu Arg Val Lys Th - #r Leu Leu Gln Met     #               105     - Val Gly Asp Ala Ala Ala Gly Met Glu Tyr Le - #u Glu Ser Lys Cys     #               120     - Cys Ile His Arg Asp Leu Ala Ala Arg Asn Cy - #s Leu Val Thr Glu     #               135     - Lys Asn Val Leu Lys Ile Ser Asp Phe Gly Me - #t Ser Arg Glu Glu     #               150     - Ala Asp Gly Val Tyr Ala Ala Ser Gly Leu Ar - #g Gln Val Pro Val     #               165     - Lys Trp Thr Ala Pro Glu Ala Leu Asn Tyr Gl - #y Arg Tyr Ser Ser     #               180     - Glu Ser Asp Val Trp Ser Phe Gly Ile Leu Le - #u Trp Glu Thr Phe     #               195     - Ser Leu Gly Ala Ser Pro Tyr Pro Asn Leu Se - #r Asn Gln Gln Thr     #               210     - Arg Glu Phe Val Glu Lys Gly Gly Arg Leu Pr - #o Cys Pro Glu Leu     #               225     - Cys Pro Asp Ala Val Phe Arg Leu Met Glu Gl - #n Cys Trp Ala Tyr     #               240     - Glu Pro Gly Gln Arg Pro Ser Phe Ser Thr Il - #e Tyr Gln Glu Leu     #               255     - Gln Ser Ile Arg Lys Arg His                     260     - (2) INFORMATION FOR SEQ ID NO:63:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 262     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: cat     #Column 71 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:63:     - Glu Asp Leu Val Leu Gly Glu Gln Ile Gly Ar - #g Gly Asn Phe Gly     #               015     - Glu Val Phe Ser Gly Arg Leu Arg Ala Asp As - #n Thr Leu Val Ala     #               030     - Val Lys Ser Cys Arg Glu Thr Leu Pro Pro As - #p Ile Lys Ala Lys     #               045     - Phe Leu Gln Glu Ala Lys Ile Leu Lys Gln Ty - #r Ser His Pro Asn     #               060     - Ile Val Arg Leu Ile Gly Val Cys Thr Gln Ly - #s Gln Pro Ile Tyr     #               075     - Ile Val Met Glu Leu Val Gln Gly Gly Asp Ph - #e Leu Thr Phe Leu     #               090     - Arg Thr Glu Gly Ala Arg Leu Arg Met Lys Th - #r Leu Leu Gln Met     #               105     - Val Gly Asp Ala Ala Ala Gly Met Glu Tyr Le - #u Glu Ser Lys Cys     #               120     - Cys Ile His Arg Asp Leu Ala Ala Arg Asn Cy - #s Leu Val Thr Glu     #               135     - Lys Asn Val Leu Lys Ile Ser Asp Phe Gly Me - #t Ser Arg Glu Glu     #               150     - Ala Asp Gly Ile Tyr Ala Ala Ser Gly Leu Ar - #g Gln Val Pro Val     #               165     - Lys Trp Thr Ala Pro Glu Ala Leu Asn Tyr Gl - #y Arg Tyr Ser Ser     #               180     - Glu Ser Asp Val Trp Ser Phe Gly Ile Leu Le - #u Trp Glu Thr Phe     #               195     - Ser Leu Gly Ala Ser Pro Tyr Pro Asn Leu Se - #r Asn Gln Gln Thr     #               210     - Arg Glu Phe Val Glu Lys Gly Gly Arg Leu Pr - #o Cys Pro Glu Leu     #               225     - Cys Pro Asp Ala Val Phe Arg Leu Met Glu Gl - #n Cys Trp Ala Tyr     #               240     - Glu Pro Gly Gln Arg Pro Ser Phe Ser Ala Il - #e Tyr Gln Glu Leu     #               255     - Gln Ser Ile Arg Lys Arg His                     260     - (2) INFORMATION FOR SEQ ID NO:64:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 262     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: chicken     #Column 72 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:64:     - Glu Asp Val Leu Leu Gly Glu Arg Ile Gly Ar - #g Gly Asn Phe Gly     #               015     - Glu Val Phe Ser Gly Arg Leu Arg Ala Asp As - #n Thr Pro Val Ala     #               030     - Val Lys Ser Cys Arg Glu Thr Leu Pro Pro Gl - #u Leu Lys Ala Lys     #               045     - Phe Leu Gln Glu Ala Arg Ile Leu Lys Gln Ty - #r Asn His Pro Asn     #               060     - Ile Val Arg Leu Ile Gly Val Cys Thr Gln Ly - #s Gln Pro Ile Tyr     #               075     - Ile Val Met Glu Leu Val Gln Gly Gly Asp Ph - #e Leu Ser Phe Leu     #               090     - Arg Ser Glu Gly Pro His Leu Lys Met Lys Gl - #u Leu Ile Lys Met     #               105     - Met Glu Asn Ala Ala Ala Gly Met Glu Tyr Le - #u Glu Ser Lys His     #               120     - Cys Ile His Arg Asp Leu Ala Ala Arg Asn Cy - #s Leu Val Thr Glu     #               135     - Lys Asn Thr Leu Lys Ile Ser Asp Phe Gly Me - #t Ser Arg Gln Glu     #               150     - Glu Asp Gly Val Tyr Ala Ser Thr Gly Met Ly - #s Gln Ile Pro Val     #               165     - Lys Trp Thr Ala Pro Glu Ala Leu Asn Tyr Gl - #y Arg Tyr Ser Ser     #               180     - Glu Ser Asp Val Trp Ser Phe Gly Ile Leu Le - #u Trp Glu Ala Phe     #               195     - Ser Leu Gly Ala Val Pro Tyr Ala Asn Leu Se - #r Asn Gln Gln Thr     #               210     - Arg Glu Ala Ile Glu Gln Gly Val Arg Leu Gl - #u Pro Pro Glu Gln     #               225     - Cys Pro Glu Asp Val Tyr Arg Leu Met Gln Ar - #g Cys Trp Glu Tyr     #               240     - Asp Pro Arg Arg Arg Pro Ser Phe Gly Ala Va - #l His Gln Asp Leu     #               255     - Ile Ala Ile Arg Lys Arg His                     260     - (2) INFORMATION FOR SEQ ID NO:65:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 265     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 74 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:65:     - Thr Glu Phe Lys Lys Ile Lys Val Leu Gly Se - #r Gly Ala Phe Gly     #               015     - Thr Val Tyr Lys Gly Leu Trp Ile Pro Glu Gl - #y Glu Lys Val Lys     #               030     - Ile Pro Val Ala Ile Lys Glu Leu Arg Glu Al - #a Thr Ser Pro Lys     #               045     - Ala Asn Lys Glu Ile Leu Asp Glu Ala Tyr Va - #l Met Ala Ser Val     #               060     - Asp Asn Pro His Val Cys Arg Leu Leu Gly Il - #e Cys Leu Thr Ser     #               075     - Thr Val Gln Leu Ile Thr Gln Leu Met Pro Ph - #e Gly Cys Leu Leu     #               090     - Asp Tyr Val Arg Glu His Lys Asp Asn Ile Gl - #y Ser Gln Tyr Leu     #               105     - Leu Asn Trp Cys Val Gln Ile Ala Lys Gly Me - #t Asn Tyr Leu Glu     #               120     - Asp Arg Arg Leu Val His Arg Asp Leu Ala Al - #a Arg Asn Val Leu     #               135     - Val Lys Thr Pro Gln His Val Lys Ile Thr As - #p Phe Gly Leu Ala     #               150     - Lys Leu Leu Gly Ala Glu Glu Lys Glu Tyr Hi - #s Glu Gly Gly Lys     #               165     - Val Pro Ile Lys Trp Met Ala Leu Glu Ser Il - #e Leu His Arg Ile     #               180     - Tyr Thr His Gln Ser Asp Val Trp Ser Tyr Gl - #y Val Thr Val Trp     #               195     - Glu Leu Met Thr Phe Gly Ser Lys Pro Tyr As - #p Gly Ile Pro Ala     #               210     - Ser Glu Ile Ser Ser Ile Leu Glu Lys Gly Gl - #u Arg Leu Pro Gln     #               225     - Pro Pro Ile Cys Thr Ile Asp Val Tyr Met Il - #e Met Val Lys Cys     #               240     - Trp Met Ile Asp Ala Asp Ser Arg Pro Lys Ph - #e Arg Glu Leu Ile     #               255     - Ile Glu Phe Ser Lys Met Ala Arg Asp Pro     #               265     - (2) INFORMATION FOR SEQ ID NO:66:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 265     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 75 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:66:     - Thr Glu Leu Arg Lys Val Lys Val Leu Gly Se - #r Gly Ala Phe Gly     #               015     - Thr Val Tyr Lys Gly Ile Trp Ile Pro Asp Gl - #y Glu Asn Val Lys     #               030     - Ile Pro Val Ala Ile Lys Val Leu Arg Glu As - #n Thr Ser Pro Lys     #               045     - Ala Asn Lys Glu Ile Leu Asp Glu Ala Tyr Va - #l Met Ala Gly Val     #               060     - Gly Ser Pro Tyr Val Ser Arg Leu Leu Gly Il - #e Cys Leu Thr Ser     #               075     - Thr Val Gln Leu Val Thr Gln Leu Met Pro Ty - #r Gly Cys Leu Leu     #               090     - Asp His Val Arg Glu Asn Arg Gly Arg Leu Gl - #y Ser Gln Asp Leu     #               105     - Leu Asn Trp Cys Met Gln Ile Ala Lys Gly Me - #t Ser Tyr Leu Glu     #               120     - Asp Val Arg Leu Val His Arg Asp Leu Ala Al - #a Arg Asn Val Leu     #               135     - Val Lys Ser Pro Asn His Val Lys Ile Thr As - #p Phe Gly Leu Ala     #               150     - Arg Leu Leu Asp Ile Asp Glu Thr Glu Tyr Hi - #s Asp Gly Gly Lys     #               165     - Val Pro Ile Lys Trp Met Ala Leu Glu Ser Il - #e Leu Arg Arg Arg     #               180     - Phe Thr His Gln Ser Asp Val Trp Ser Tyr Gl - #y Val Thr Val Trp     #               195     - Glu Leu Met Thr Phe Gly Ala Lys Pro Tyr As - #p Gly Ile Pro Ala     #               210     - Arg Glu Ile Pro Asp Leu Leu Glu Lys Gly Gl - #u Arg Leu Pro Gln     #               225     - Pro Pro Ile Cys Thr Ile Asp Val Tyr Met Il - #e Met Val Lys Cys     #               240     - Trp Met Ile Asp Ser Glu Cys Arg Pro Arg Ph - #e Arg Glu Leu Val     #               255     - Ser Glu Phe Ser Arg Met Ala Arg Asp Pro     #               265     - (2) INFORMATION FOR SEQ ID NO:67:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 264     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:     #melanogaster ORGANISM: Drosophila     #Column 76 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:67:     - Ala Glu Leu Arg Lys Gly Gly Val Leu Gly Me - #t Gly Ala Phe Gly     #               015     - Arg Val Tyr Lys Gly Val Trp Val Pro Glu Gl - #y Glu Asn Val Lys     #               030     - Ile Pro Val Ala Ile Lys Glu Leu Leu Lys Se - #r Thr Gly Ala Glu     #               045     - Ser Ser Glu Glu Phe Leu Arg Glu Ala Tyr Il - #e Met Ala Ser Glu     #               060     - Glu His Val Asn Leu Leu Lys Leu Leu Ala Va - #l Cys Met Ser Ser     #               075     - Gln Met Met Leu Ile Thr Gln Leu Met Pro Le - #u Gly Cys Leu Leu     #               090     - Asp Tyr Val Arg Asn Asn Arg Asp Lys Ile Se - #r Ser Lys Ala Leu     #               105     - Leu Asn Trp Ser Thr Gln Ile Ala Lys Gly Me - #t Ser Tyr Leu Glu     #               120     - Glu Lys Arg Leu Val His Arg Asp Leu Ala Al - #a Arg Asn Val Leu     #               135     - Val Arg Leu Leu Ala Gly Glu Asp His Asp Ph - #e Gly Leu Ala Lys     #               150     - Leu Leu Ser Ser Asp Ser Asn Glu Tyr Lys Al - #a Gly Gly Lys Met     #               165     - Pro Ile Lys Trp Leu Ala Leu Glu Cys Ile Ar - #g Asn Arg Val Phe     #               180     - Thr Ser Lys Ser Asp Val Trp Ala Phe Gly Va - #l Thr Ile Trp Glu     #               195     - Leu Leu Thr Phe Gly Gln Arg Pro His Glu As - #n Ile Pro Ala Lys     #               210     - Asp Ile Pro Asp Leu Ile Glu Val Gly Leu Ly - #s Leu Glu Gln Pro     #               225     - Glu Ile Cys Ser Leu Asp Ile Tyr Cys Thr Le - #u Leu Ser Cys Trp     #               240     - His Leu Asp Ala Ala Met Arg Pro Thr Phe Ly - #s Gln Leu Thr Thr     #               255     - Val Phe Ala Glu Phe Ala Arg Asp Pro                     260     - (2) INFORMATION FOR SEQ ID NO:68:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 269     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 78 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:68:     - Glu Lys Ile Thr Leu Leu Arg Glu Leu Gly Gl - #n Gly Ser Phe Gly     #               015     - Met Val Tyr Glu Gly Asn Ala Arg Asp Ile Il - #e Lys Gly Glu Ala     #               030     - Glu Thr Arg Val Ala Val Lys Thr Val Asn Gl - #u Ser Ala Ser Leu     #               045     - Arg Glu Arg Ile Glu Phe Leu Asn Glu Ala Se - #r Val Met Lys Gly     #               060     - Phe Thr Cys His His Val Val Arg Leu Leu Gl - #y Val Val Ser Lys     #               075     - Gly Gln Pro Thr Leu Val Val Met Glu Leu Me - #t Ala His Gly Asp     #               090     - Leu Lys Ser Tyr Leu Arg Ser Leu Arg Pro Gl - #u Ala Glu Pro Thr     #               105     - Leu Gln Glu Met Ile Gln Met Ala Ala Glu Il - #e Ala Asp Gly Met     #               120     - Ala Tyr Leu Asn Ala Lys Lys Phe Val His Ar - #g Asp Leu Ala Ala     #               135     - Arg Asn Cys Met Val Ala His Asp Phe Thr Va - #l Lys Ile Gly Asp     #               150     - Phe Gly Met Thr Arg Asp Ile Tyr Glu Thr As - #p Tyr Tyr Arg Lys     #               165     - Gly Lys Gly Leu Leu Pro Val Arg Trp Met Al - #a Pro Glu Ser Leu     #               180     - Lys Asp Gly Val Phe Thr Thr Ser Ser Asp Me - #t Trp Ser Phe Gly     #               195     - Val Val Leu Trp Glu Ile Thr Ser Leu Ala Gl - #u Gln Pro Tyr Gln     #               210     - Gly Leu Ser Asn Glu Gln Val Leu Lys Phe Va - #l Met Asp Gly Gly     #               225     - Tyr Leu Asp Gln Pro Asp Asn Cys Pro Glu Ar - #g Val Thr Asp Leu     #               240     - Met Arg Met Cys Trp Gln Phe Asn Pro Asn Me - #t Arg Pro Thr Phe     #               255     - Leu Glu Ile Val Asn Leu Leu Lys Asp Asp Le - #u His Pro Ser     #               265     - (2) INFORMATION FOR SEQ ID NO:69:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 269     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 79 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:69:     - Glu Lys Ile Thr Met Ser Arg Glu Leu Gly Gl - #n Gly Ser Phe Gly     #               015     - Met Val Tyr Glu Gly Val Ala Lys Gly Val Va - #l Lys Asp Glu Pro     #               030     - Glu Thr Arg Val Ala Ile Lys Thr Val Asn Gl - #u Ala Ala Ser Met     #               045     - Arg Glu Arg Ile Glu Phe Leu Asn Glu Ala Se - #r Val Met Lys Glu     #               060     - Phe Asn Cys His His Val Val Arg Leu Leu Gl - #y Val Val Ser Gln     #               075     - Gly Gln Pro Thr Leu Val Ile Met Glu Leu Me - #t Thr Arg Gly Asp     #               090     - Leu Lys Ser Tyr Leu Arg Ser Leu Arg Pro Gl - #u Met Glu Pro Ser     #               105     - Leu Ser Lys Met Ile Gln Met Ala Gly Glu Il - #e Ala Asp Gly Met     #               120     - Ala Tyr Leu Asn Ala Asn Lys Phe Val His Ar - #g Asp Leu Ala Ala     #               135     - Arg Asn Cys Met Val Ala Glu Asp Phe Thr Va - #l Lys Ile Gly Asp     #               150     - Phe Gly Met Thr Arg Asp Ile Tyr Glu Thr As - #p Tyr Tyr Arg Lys     #               165     - Gly Lys Gly Leu Leu Pro Val Arg Trp Met Se - #r Pro Glu Ser Leu     #               180     - Lys Asp Gly Val Phe Thr Thr Tyr Ser Asp Va - #l Trp Ser Phe Gly     #               195     - Val Val Leu Trp Glu Ile Ala Thr Leu Ala Gl - #u Gln Pro Tyr Gln     #               210     - Gly Leu Ser Asn Glu Gln Val Leu Arg Phe Va - #l Met Glu Gly Gly     #               225     - Leu Leu Asp Lys Pro Asp Asn Cys Pro Asp Me - #t Leu Phe Glu Leu     #               240     - Met Arg Met Cys Trp Gln Tyr Asn Pro Lys Me - #t Arg Pro Ser Phe     #               255     - Leu Glu Ile Ile Ser Ser Ile Lys Glu Glu Me - #t Glu Pro Gly     #               265     - (2) INFORMATION FOR SEQ ID NO:70:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 269     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:     #melanogaster ORGANISM: Drosophila     #Column 80 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:70:     - Glu Asn Ile Ile Gln Leu Ala Pro Leu Gly Gl - #n Gly Ser Phe Gly     #               015     - Met Val Tyr Glu Gly Ile Leu Lys Ser Phe Pr - #o Pro Asn Gly Val     #               030     - Asp Arg Glu Cys Ala Ile Lys Thr Val Asn Gl - #u Asn Ala Thr Asp     #               045     - Arg Glu Arg Thr Asn Phe Leu Ser Glu Ala Se - #r Val Met Lys Glu     #               060     - Phe Asp Thr Tyr His Val Val Arg Leu Leu Gl - #y Val Cys Ser Arg     #               075     - Gly Gln Pro Ala Leu Val Val Met Glu Leu Me - #t Lys Lys Val Phe     #               090     - Leu Lys Ser Tyr Leu Arg Ala His Arg Pro Ar - #g Ser Gly Pro Thr     #               105     - Tyr Gly Arg Ile Tyr Gln Val Ala Ile Glu Il - #e Ala Asp Gly Met     #               120     - Ala Tyr Leu Ala Ala Lys Lys Phe Val His Ar - #g Asp Leu Ala Ala     #               135     - Arg Asn Cys Met Val Ala Asp Asp Leu Thr Va - #l Lys Ile Gly Asp     #               150     - Phe Gly Met Thr Arg Asp Ile Tyr Glu Thr As - #p Tyr Tyr Arg Lys     #               165     - Thr Lys Gly Leu Leu Pro Val Arg Trp Met Pr - #o Pro Glu Ser Leu     #               180     - Arg Asp Gly Val Tyr Ser Ser Ala Ser Asp Va - #l Phe Ser Phe Gly     #               195     - Val Val Leu Trp Glu Met Ala Thr Leu Ala Al - #a Gln Pro Tyr Gln     #               210     - Gly Leu Ser Asn Glu Gln Val Leu Arg Tyr Va - #l Ile Asp Gly Gly     #               225     - Val Met Glu Arg Pro Glu Asn Cys Pro Asp Ph - #e Leu His Lys Leu     #               240     - Met Gln Arg Cys Trp His His Arg Ser Ser Al - #a Arg Pro Ser Phe     #               255     - Leu Asp Ile Ile Ala Tyr Leu Glu Pro Gln Cy - #s Pro Asn Ser     #               265     - (2) INFORMATION FOR SEQ ID NO:71:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 275     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 81 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:71:     - Glu Lys Leu Thr Leu Arg Leu Leu Leu Gly Se - #r Gly Ala Phe Gly     #               015     - Glu Val Tyr Glu Gly Thr Ala Val Asp Ile Le - #u Gly Val Gly Ser     #               030     - Gly Glu Ile Lys Val Ala Val Lys Thr Leu Ly - #s Lys Gly Ser Thr     #               045     - Asp Gln Glu Lys Ile Glu Phe Leu Lys Glu Al - #a His Leu Met Ser     #               060     - Lys Phe Asn His Pro Asn Ile Leu Lys Gln Le - #u Gly Val Cys Leu     #               075     - Leu Asn Glu Pro Gln Tyr Ile Ile Leu Glu Le - #u Met Glu Gly Gly     #               090     - Asp Leu Leu Thr Tyr Leu Arg Lys Ala Arg Me - #t Ala Thr Phe Leu     #               105     - Thr Leu Val Asp Leu Val Asp Leu Cys Val As - #p Ile Ser Lys Gly     #               120     - Cys Val Tyr Leu Glu Arg Met His Phe Ile Hi - #s Arg Asp Leu Ala     #               135     - Ala Arg Asn Cys Leu Val Ser Val Lys Asp Ty - #r Thr Ser Pro Arg     #               150     - Ile Val Lys Ile Gly Asp Phe Gly Leu Ala Ar - #g Asp Ile Tyr Lys     #               165     - Asn Asp Tyr Tyr Arg Lys Gly Glu Gly Leu Le - #u Pro Val Arg Trp     #               180     - Met Ala Pro Glu Ser Leu Met Asp Gly Ile Ph - #e Thr Thr Gln Ser     #               195     - Asp Val Trp Ser Phe Gly Ile Leu Ile Trp Gl - #u Ile Leu Thr Leu     #               210     - Gly His Gln Pro Tyr Pro Ala His Ser Asn Le - #u Asp Val Leu Asn     #               225     - Tyr Val Gln Thr Gly Gly Arg Leu Glu Pro Pr - #o Arg Asn Cys Pro     #               240     - Asp Asp Leu Trp Asn Leu Met Thr Gln Cys Tr - #p Ala Gln Glu Pro     #               255     - Asp Gln Arg Pro Thr Phe His Arg Ile Gln As - #p Gln Leu Gln Leu     #               270     - Phe Arg Asn Phe Phe                     275     - (2) INFORMATION FOR SEQ ID NO:72:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 276     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: chicken     #Column 82 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:72:     - Asp Lys Leu Asn Leu His Lys Leu Leu Gly Se - #r Gly Ala Phe Gly     #               015     - Glu Val Tyr Glu Gly Thr Ala Leu Asp Ile Le - #u Ala Asp Gly Ser     #               030     - Gly Glu Ser Arg Val Ala Val Lys Thr Leu Ly - #s Arg Gly Ala Thr     #               045     - Asp Gln Glu Lys Ser Glu Phe Leu Lys Glu Al - #a His Leu Met Ser     #               060     - Lys Phe Asp His Pro His Ile Leu Lys Leu Le - #u Gly Val Cys Leu     #               075     - Leu Asn Glu Pro Gln Tyr Leu Ile Leu Glu Le - #u Met Glu Gly Gly     #               090     - Asp Leu Leu Ser Tyr Leu Arg Gly Ala Arg Ly - #s Gln Lys Phe Leu     #               105     - Thr Leu Thr Asp Leu Leu Asp Ile Cys Leu As - #p Ile Cys Lys Gly     #               120     - Cys Val Tyr Leu Glu Lys Met Arg Phe Ile Hi - #s Arg Asp Leu Ala     #               135     - Ala Arg Asn Cys Leu Val Ser Glu Lys Gln Ty - #r Gly Ser Cys Ser     #               150     - Arg Val Val Lys Ile Gly Asp Phe Gly Leu Al - #a Arg Asp Ile Tyr     #               165     - Lys Asn Asp Tyr Tyr Arg Lys Gly Glu Gly Le - #u Leu Pro Val Arg     #               180     - Trp Met Ala Pro Glu Ser Leu Ile Asp Gly Va - #l Phe Thr Asn His     #               195     - Ser Asp Val Met Ala Phe Gly Val Leu Val Tr - #p Glu Thr Leu Thr     #               210     - Leu Gly Gln Gln Pro Tyr Pro Gly Leu Ser As - #n Ile Glu Val Leu     #               225     - His His Val Arg Ser Gly Gly Arg Leu Glu Se - #r Pro Asn Asn Cys     #               240     - Pro Asp Asp Ile Arg Asp Leu Met Thr Arg Cy - #s Trp Ala Gln Asp     #               255     - Pro His Asn Arg Pro Thr Phe Phe Tyr Ile Gl - #n His Lys Leu Gln     #               270     - Glu Ile Arg His Ser Pro                     275     - (2) INFORMATION FOR SEQ ID NO:73:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 273     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:     #melanogaster ORGANISM: Drosophila     #Column 83 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:73:     - Ser Gln Leu Lys Leu Leu Arg Phe Leu Gly Se - #r Gly Ala Phe Gly     #               015     - Glu Val Tyr Glu Gly Gln Leu Lys Thr Glu As - #p Ser Glu Glu Pro     #               030     - Gln Arg Val Ala Ile Lys Ser Leu Arg Lys Gl - #y Ala Ser Glu Phe     #               045     - Ala Glu Leu Leu Gln Glu Ala Gln Leu Met Se - #r Asn Phe Lys His     #               060     - Glu Asn Ile Val Arg Leu Val Gly Ile Cys Ph - #e Asp Thr Glu Pro     #               075     - Ile Ser Leu Ile Met Glu His Met Glu Ala Gl - #y Asp Leu Leu Ser     #               090     - Tyr Leu Arg Ala Ala Arg Ala Thr Ser Thr Le - #u Ser Leu Ser Glu     #               105     - Leu Leu Ala Met Cys Ile Asp Val Ala Asn Gl - #y Cys Ser Tyr Leu     #               120     - Glu Asp Met His Phe Val His Arg Asp Leu Al - #a Cys Arg Asn Cys     #               135     - Leu Val Thr Glu Ser Thr Gly Ser Thr Asp Ar - #g Arg Arg Thr Val     #               150     - Lys Ile Gly Asp Phe Gly Leu Ala Arg Asp Il - #e Tyr Lys Ser Asp     #               165     - Tyr Tyr Arg Lys Gly Glu Gly Leu Leu Pro Va - #l Arg Trp Met Ser     #               180     - Pro Glu Ser Leu Val Asp Gly Leu Phe Thr Th - #r Gln Ser Asp Val     #               195     - Trp Ala Phe Gly Val Leu Cys Trp Glu Ile Le - #u Thr Leu Gly Gln     #               210     - Gln Pro Tyr Ala Ala Arg Asn Asn Phe Glu Va - #l Leu Ala His Val     #               225     - Lys Glu Gly Gly Arg Leu Gln Gln Pro Pro Me - #t Cys Thr Glu Lys     #               240     - Leu Tyr Ser Leu Leu Leu Leu Cys Trp Arg Th - #r Asp Pro Trp Glu     #               255     - Arg Pro Ser Phe Arg Arg Cys Tyr Asn Thr Le - #u His Ala Ile Ser     #               270     - Thr Asp Leu     - (2) INFORMATION FOR SEQ ID NO:74:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 268     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 84 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:74:     - Arg Asp Ile Val Leu Lys Trp Glu Leu Gly Gl - #u Gly Ala Phe Gly     #               015     - Lys Val Phe Leu Ala Glu Cys His Asn Leu Le - #u Pro Glu Gln Asp     #               030     - Lys Met Leu Val Ala Val Lys Ala Leu Lys Gl - #u Ala Ser Glu Ser     #               045     - Ala Arg Gln Asp Phe Gln Arg Glu Val Glu Le - #u Leu Thr Met Leu     #               060     - Gln His Gln His Ile Val Arg Phe Phe Gly Va - #l Cys Thr Glu Gly     #               075     - Arg Pro Leu Leu Met Val Phe Glu Tyr Met Ar - #g His Gly Asp Leu     #               090     - Asn Arg Phe Leu Arg Ser His Gly Pro Asp Al - #a Lys Leu Gly Leu     #               105     - Gly Gln Leu Leu Ala Val Ala Ser Gln Val Al - #a Ala Gly Met Val     #               120     - Tyr Leu Ala Gly Leu His Phe Val His Arg As - #p Leu Ala Thr Arg     #               135     - Asn Cys Leu Val Gly Gln Gly Leu Val Val Ly - #s Ile Gly Asp Phe     #               150     - Gly Met Ser Arg Asp Ile Tyr Ser Thr Asp Ty - #r Tyr Arg Val Gly     #               165     - Arg Thr Met Leu Pro Ile Arg Trp Met Pro Pr - #o Glu Ser Ile Leu     #               180     - Tyr Arg Lys Phe Thr Thr Glu Ser Asp Val Tr - #p Ser Phe Gly Val     #               195     - Val Leu Trp Glu Ile Phe Thr Tyr Gly Lys Gl - #n Pro Trp Tyr Gln     #               210     - Leu Ser Asn Thr Glu Ala Ile Asp Cys Ile Th - #r Gln Gly Arg Glu     #               225     - Leu Glu Arg Pro Arg Ala Cys Pro Pro Glu Va - #l Tyr Ala Ile Met     #               240     - Arg Gly Cys Trp Gln Arg Glu Pro Gln Gln Ar - #g Pro Ser Ile Lys     #               255     - Asp Val His Ala Arg Leu Gln Ala Leu Ala Gl - #n Ala Pro     #               265     - (2) INFORMATION FOR SEQ ID NO:75:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 266     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 85 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:75:     - Leu Ile Val His Phe Asn Glu Val Ile Gly Ar - #g Gly His Phe Gly     #               015     - Cys Val Tyr His Gly Thr Leu Leu Asp Asn As - #p Gly Lys Lys Ile     #               030     - His Cys Ala Val Lys Ser Leu Asn Arg Ile Th - #r Asp Ile Gly Glu     #               045     - Val Ser Gln Phe Leu Thr Glu Gly Ile Ile Me - #t Lys Asp Phe Ser     #               060     - His Pro Asn Val Leu Ser Leu Leu Gly Ile Cy - #s Leu Arg Ser Glu     #               075     - Gly Ser Pro Leu Val Val Leu Pro Tyr Met Ly - #s His Gly Asp Leu     #               090     - Arg Asn Phe Ile Arg Asn Glu Thr His Asn Pr - #o Thr Val Lys Asp     #               105     - Leu Ile Gly Phe Gly Leu Gln Val Ala Lys Al - #a Met Lys Tyr Leu     #               120     - Ala Ser Lys Lys Phe Val His Arg Asp Leu Al - #a Ala Arg Asn Cys     #               135     - Met Leu Asp Glu Lys Phe Thr Val Lys Val Al - #a Asp Phe Gly Leu     #               150     - Ala Arg Asp Met Tyr Asp Lys Glu Tyr Tyr Se - #r Val Thr Gly Ala     #               165     - Lys Leu Pro Val Lys Trp Met Ala Leu Glu Se - #r Leu Gln Thr Gln     #               180     - Lys Phe Thr Thr Lys Ser Asp Val Trp Ser Ph - #e Gly Val Val Leu     #               195     - Trp Glu Leu Met Thr Arg Gly Ala Pro Pro Ty - #r Pro Asp Val Asn     #               210     - Thr Phe Asp Ile Thr Val Tyr Leu Leu Gln Gl - #y Arg Arg Leu Leu     #               225     - Gln Pro Glu Tyr Cys Pro Asp Pro Leu Tyr Gl - #u Val Met Leu Lys     #               240     - Cys Trp His Pro Lys Ala Glu Met Arg Pro Se - #r Phe Ser Glu Leu     #               255     - Val Ser Arg Ile Ser Ala Ile Phe Ser Thr Ph - #e     #               265     - (2) INFORMATION FOR SEQ ID NO:76:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 270     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: mouse     #Column 87 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:76:     - Asp Gln Leu Val Ile Gly Arg Thr Leu Gly Se - #r Gly Ala Phe Gly     #               015     - Gln Val Val Glu Ala Thr Ala His Asp Leu Se - #r His Ser Gln Ala     #               030     - Thr Met Lys Val Ala Val Lys Met Leu Lys Se - #r Thr Ala Arg Ser     #               045     - Ser Glu Lys Gln Ala Leu Met Ser Glu Leu Ly - #s Ile Met Ser His     #               060     - Leu Gly Pro His Leu His Val Val Asn Leu Le - #u Gly Ala Cys Thr     #               075     - Lys Gly Gly Pro Ile Tyr Ile Ile Thr Glu Ty - #r Cys Arg Tyr Gly     #               090     - Asp Leu Val Asp Tyr Leu His Arg Asn Lys Hi - #s Thr Phe Leu Leu     #               105     - Ser Tyr Thr Asp Leu Val Gly Phe Ser Tyr Gl - #n Val Ala Asn Gly     #               120     - Met Asp Phe Leu Ala Ser Lys Asn Cys Val Hi - #s Arg Asp Leu Ala     #               135     - Ala Arg Asn Val Leu Ile Cys Glu Gly Lys Le - #u Val Lys Ile Cys     #               150     - Asp Phe Gly Leu Ala Arg Asp Ile Met Arg As - #p Ser Asn Tyr Ile     #               165     - Ser Gly Ser Thr Tyr Leu Pro Leu Lys Trp Me - #t Ala Pro Glu Ser     #               180     - Ile Phe Asn Ser Leu Tyr Thr Thr Leu Ser As - #p Val Trp Ser Phe     #               195     - Gly Ile Leu Leu Trp Glu Ile Phe Thr Leu Gl - #y Gly Thr Pro Tyr     #               210     - Pro Glu Pro Met Asn Asp Gln Phe Tyr Asn Al - #a Ile Lys Arg Gly     #               225     - Tyr Arg Met Ala Gln Pro Ala His Ala Ser As - #p Glu Ile Tyr Glu     #               240     - Ile Met Gln Lys Cys Trp Glu Glu Lys Phe Gl - #u Thr Arg Pro Pro     #               255     - Phe Ser Gln Leu Val Leu Leu Leu Glu Arg Le - #u Leu Gly Glu Gly     #               270     - (2) INFORMATION FOR SEQ ID NO:77:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 270     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 88 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:77:     - Asn Asn Leu Gln Phe Gly Lys Thr Leu Gly Al - #a Gly Ala Phe Gly     #               015     - Lys Val Val Glu Ala Thr Ala Phe Asp Leu Gl - #y Lys Glu Asp Ala     #               030     - Val Leu Lys Val Ala Val Lys Met Leu Lys Se - #r Thr Ala His Ala     #               045     - Asp Glu Lys Glu Ala Leu Met Ser Glu Leu Ly - #s Ile Met Ser His     #               060     - Leu Gly Gln His Glu His Ile Val Asn Leu Le - #u Gly Ala Cys Thr     #               075     - His Gly Gly Pro Val Leu Val Ile Thr Glu Ty - #r Cys Cys Tyr Gly     #               090     - Asp Leu Leu Asn Phe Leu Arg Arg Lys Ala Gl - #u Ala Met Leu Leu     #               105     - Glu Leu Arg Asp Leu Leu His Phe Ser Ser Gl - #n Val Ala Gln Gly     #               120     - Met Ala Phe Leu Ala Ser Lys Asn Cys Ile Hi - #s Arg Asp Leu Ala     #               135     - Ala Arg Asn Val Leu Leu Thr Asn Gly His Va - #l Ala Lys Ile Gly     #               150     - Asp Phe Gly Leu Ala Arg Asp Ile Met Asn As - #p Ser Asn Tyr Ile     #               165     - Val Gly Asn Ala Arg Leu Pro Val Lys Trp Me - #t Ala Pro Glu Ser     #               180     - Ile Phe Asp Cys Val Tyr Thr Val Gln Ser As - #p Val Trp Ser Tyr     #               195     - Gly Ile Leu Leu Trp Glu Ile Phe Ser Leu Gl - #y Leu Asn Pro Tyr     #               210     - Pro Gly Leu Val Asn Ser Lys Phe Tyr Lys Le - #u Val Lys Asp Gly     #               225     - Tyr Gln Met Ala Gln Pro Ala Phe Ala Pro Ly - #s Asn Ile Tyr Ser     #               240     - Ile Met Gln Ala Cys Trp Ala Leu Glu Pro Th - #r His Arg Pro Thr     #               255     - Phe Gln Gln Ile Cys Ser Phe Leu Gln Glu Gl - #n Ala Gln Glu Asp     #               270     - (2) INFORMATION FOR SEQ ID NO:78:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 270     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 89 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:78:     - Asn Arg Leu Ser Phe Gly Lys Thr Leu Gly Al - #a Gly Ala Phe Gly     #               015     - Lys Val Val Glu Ala Thr Ala Tyr Asp Leu Il - #e His Ser Asp Ala     #               030     - Ala Met Thr Val Ala Val Lys Met Leu Lys Pr - #o Ser Ala His Leu     #               045     - Thr Glu Arg Glu Ala Leu Met Ser Glu Leu Ly - #s Val Leu Ser Tyr     #               060     - Leu Gly Asn His Met His Ile Val Asn Leu Le - #u Gly Ala Cys Thr     #               075     - Ile Gly Gly Pro Thr Leu Val Ile Thr Glu Ty - #r Cys Cys Tyr Gly     #               090     - Asp Leu Leu Asn Phe Leu Arg Arg Lys Arg As - #p Ser Phe Ile Leu     #               105     - Asp Leu Glu Asp Leu Leu Ser Phe Ser Tyr Gl - #n Val Ala Lys Gly     #               120     - Met Ala Phe Leu Ala Ser Lys Asn Cys Ile Hi - #s Arg Asp Leu Ala     #               135     - Ala Arg Asn Ile Leu Leu Thr His Gly Arg Il - #e Thr Lys Ile Cys     #               150     - Asp Phe Gly Leu Ala Arg Asp Ile Lys Asn As - #p Ser Asn Tyr Val     #               165     - Val Gly Asn Ala Arg Leu Pro Val Lys Trp Me - #t Ala Pro Glu Ser     #               180     - Ile Phe Asn Cys Val Tyr Thr Phe Glu Ser As - #p Val Trp Ser Tyr     #               195     - Gly Ile Phe Leu Trp Glu Leu Phe Ser Leu Gl - #y Ser Ser Pro Tyr     #               210     - Pro Gly Pro Val Asp Ser Lys Phe Tyr Lys Me - #t Ile Lys Glu Gly     #               225     - Phe Arg Met Leu Ser Pro Glu His Ala Pro Al - #a Glu Met Tyr Asp     #               240     - Ile Met Lys Thr Cys Trp Asp Ala Asp Pro Le - #u Lys Arg Pro Thr     #               255     - Phe Lys Gln Ile Val Gln Leu Ile Glu Lys Gl - #n Ile Ser Glu Ser     #               270     - (2) INFORMATION FOR SEQ ID NO:79:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 269     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #Column 90 FEATURE: Protein kinase; Table 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Hanks, S. - # K.                    Quinn, A. - # M.                    Hunter, T - #.     #kinase familyTITLE: The protein     -           (C) JOURNAL: Science     -           (D) VOLUME: 241     -           (F) PAGES: 42-52     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:79:     - Lys Asn Leu Val Leu Gly Lys Thr Leu Gly Gl - #u Gly Glu Phe Gly     #               015     - Lys Val Val Lys Ala Thr Ala Phe His Leu Ly - #s Lys Arg Ala Gly     #               030     - Tyr Thr Thr Val Ala Val Lys Met Leu Lys Gl - #u Asn Ala Ser Pro     #               045     - Ser Glu Leu Arg Asp Leu Leu Ser Glu Phe As - #n Val Leu Lys Gln     #               060     - Val Asn His Pro His Val Ile Asn Leu Tyr Gl - #y Ala Cys Ser Gln     #               075     - Asp Gly Pro Leu Leu Leu Ile Val Glu Tyr Al - #a Lys Tyr Gly Ser     #               090     - Leu Arg Gly Phe Leu Arg Glu Ser Arg Lys Va - #l Met Pro Leu Thr     #               105     - Met Gly Asp Leu Ile Ser Phe Ala Trp Gln Il - #e Ser Gln Gly Met     #               120     - Gln Tyr Leu Ala Glu Met Lys Leu Val His Ar - #g Asp Leu Ala Ala     #               135     - Arg Asn Ile Leu Val Ala Glu Gly Arg Lys Me - #t Lys Ile Ser Asp     #               150     - Phe Gly Leu Ser Arg Asp Val Tyr Glu Glu As - #p Ser Tyr Val Lys     #               165     - Ser Gln Gly Arg Ile Pro Val Lys Trp Met Al - #a Ile Glu Ser Leu     #               180     - Phe Asp His Ile Tyr Thr Thr Gln Ser Asp Va - #l Trp Ser Phe Gly     #               195     - Val Leu Leu Trp Glu Ile Val Thr Leu Gly Gl - #y Asn Pro Tyr Pro     #               210     - Gly Ile Pro Pro Glu Arg Leu Phe Asn Leu Le - #u Lys Thr Gly His     #               225     - Arg Met Glu Arg Pro Asp Asn Cys Ser Glu Gl - #u Met Tyr Arg Leu     #               240     - Met Leu Gln Cys Trp Lys Gln Glu Pro Asp Ly - #s Arg Pro Val Phe     #               255     - Ala Asp Ile Ser Lys Asp Leu Glu Lys Met Me - #t Val Lys Arg     #               265     - (2) INFORMATION FOR SEQ ID NO:80:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 374     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: horse     #3 Column 1FEATURE: Alcohol dehydrogenase, Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #H.              Joernvall,     #M.            Persson,     #J.            Jeffery,               (B) TITLE: Alcohol dehy - #drogenases     #of the National Academy of Sciences,     #     USA               (D) VOLUME: 78     -           (F) PAGES: 4226-4230     -           (G) DATE: 1981     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:80:     - Ser Thr Ala Gly Lys Val Ile Lys Cys Lys Al - #a Ala Val Leu Trp     #               015     - Glu Glu Lys Lys Pro Phe Ser Ile Glu Glu Va - #l Glu Val Ala Pro     #               030     - Pro Lys Ala His Glu Val Arg Ile Lys Met Va - #l Ala Thr Gly Ile     #               045     - Cys Arg Ser Asp Asp His Val Val Ser Gly Th - #r Leu Val Thr Pro     #               060     - Leu Pro Val Ile Ala Gly His Glu Ala Ala Gl - #y Ile Val Glu Ser     #               075     - Ile Gly Glu Gly Val Thr Thr Val Arg Pro Gl - #y Asp Lys Val Ile     #               090     - Pro Leu Phe Thr Pro Gln Cys Gly Lys Cys Ar - #g Val Cys Lys His     #               105     - Pro Glu Gly Asn Phe Cys Leu Lys Asn Asp Le - #u Ser Met Pro Arg     #               120     - Gly Thr Met Gln Asp Gly Thr Ser Arg Phe Th - #r Cys Arg Gly Lys     #               135     - Pro Ile His His Phe Leu Gly Thr Ser Thr Ph - #e Ser Gln Tyr Thr     #               150     - Val Val Asp Glu Ile Ser Val Ala Lys Ile As - #p Ala Ala Ser Pro     #               165     - Leu Glu Lys Val Cys Leu Ile Gly Cys Gly Ph - #e Ser Thr Gly Tyr     #               180     - Gly Ser Ala Val Lys Val Ala Lys Val Thr Gl - #n Gly Ser Thr Cys     #               195     - Ala Val Phe Gly Leu Gly Gly Val Gly Leu Se - #r Val Ile Met Gly     #               210     - Cys Lys Ala Ala Gly Ala Ala Ala Ala Arg Il - #e Ile Gly Val Asp     #               225     - Ile Asn Lys Asp Lys Phe Ala Lys Glu Val Gl - #y Ala Thr Glu Cys     #               240     - Val Asn Pro Gln Asp Tyr Lys Lys Pro Ile Gl - #n Glu Val Leu Thr     #               255     - Glu Met Ser Asn Gly Gly Val Asp Phe Ser Ph - #e Glu Val Ile Gly     #               270     - Arg Leu Asp Thr Met Val Thr Ala Leu Ser Cy - #s Cys Gln Glu Ala     #               285     - Tyr Gly Val Ser Val Ile Val Gly Val Pro Pr - #o Asp Ser Gln Asn     #               300     - Leu Ser Met Asn Pro Met Leu Leu Leu Ser Gl - #y Arg Thr Trp Lys     #               315     - Gly Ala Ile Phe Gly Gly Phe Lys Ser Lys As - #p Ser Val Pro Lys     #               330     - Leu Val Ala Asp Phe Met Ala Lys Lys Phe Al - #a Leu Asp Pro Leu     #               345     - Ile Thr His Val Leu Pro Phe Glu Lys Ile As - #n Glu Gly Phe Asp     #               360     - Leu Leu Arg Ser Gly Glu Ser Ile Arg Thr Il - #e Leu Thr Phe     #               370     - (2) INFORMATION FOR SEQ ID NO:81:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 374     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: horse     #3 Column 2FEATURE: Alcohol dehydrogenase, Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #H.              Joernvall,     #M.            Persson,     #J.            Jeffery,               (B) TITLE: Alcohol dehy - #drogenases     #of the National Academy of Sciences,     #     USA               (D) VOLUME: 78     -           (F) PAGES: 4226-4230     -           (G) DATE: 1981     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:81:     - Ser Thr Ala Gly Lys Val Ile Lys Cys Lys Al - #a Ala Val Leu Trp     #               015     - Glu Asn Lys Lys Pro Phe Ser Ile Glu Glu Va - #l Glu Val Ala Pro     #               030     - Pro Lys Ala His Glu Val Arg Ile Lys Met Va - #l Ala Thr Gly Ile     #               045     - Cys Arg Ser Asp Asp His Val Val Ser Gly Th - #r Leu Val Thr Pro     #               060     - Leu Pro Val Ile Ala Gly His Glu Ala Ala Gl - #y Ile Val Glu Ser     #               075     - Ile Gly Glu Gly Val Thr Thr Val Arg Pro Gl - #y Asp Lys Val Ile     #               090     - Pro Leu Phe Ile Pro Gln Cys Gly Lys Cys Se - #r Val Cys Lys His     #               105     - Pro Glu Gly Asn Leu Cys Leu Lys Asn Ser Le - #u Ser Met Pro Arg     #               120     - Gly Thr Met Gln Asp Gly Thr Ser Arg Phe Th - #r Cys Arg Gly Lys     #               135     - Pro Ile His His Phe Leu Gly Thr Ser Thr Ph - #e Ser Gln Tyr Thr     #               150     - Val Val Asp Glu Ile Ser Val Ala Lys Ile As - #p Ala Ala Ser Pro     #               165     - Leu Glu Lys Val Cys Leu Ile Gly Cys Gly Ph - #e Ser Thr Gly Tyr     #               180     - Gly Ser Ala Val Lys Val Ala Lys Val Thr Gl - #n Gly Ser Thr Cys     #               195     - Ala Val Phe Gly Leu Gly Gly Val Gly Leu Se - #r Val Ile Met Gly     #               210     - Cys Lys Ala Ala Gly Ala Ala Ala Ala Arg Il - #e Ile Gly Val Asp     #               225     - Ile Asn Lys Asp Lys Phe Ala Lys Glu Val Gl - #y Ala Thr Glu Cys     #               240     - Val Asn Pro Gln Asp Tyr Lys Lys Pro Ile Gl - #n Glu Val Leu Thr     #               255     - Glu Met Ser Asn Gly Gly Val Asp Phe Ser Ph - #e Glu Val Ile Gly     #               270     - Arg Leu Asp Thr Met Val Thr Ala Leu Ser Cy - #s Cys Gln Glu Ala     #               285     - Tyr Gly Val Ser Val Ile Val Gly Val Pro Pr - #o Asp Ser Gln Asn     #               300     - Leu Ser Met Asn Pro Met Leu Leu Leu Ser Gl - #y Arg Thr Trp Lys     #               315     - Gly Ala Ile Phe Gly Gly Phe Lys Ser Lys As - #p Ser Val Pro Lys     #               330     - Leu Val Ala Asp Phe Met Ala Lys Lys Phe Al - #a Leu Asp Pro Leu     #               345     - Ile Thr His Val Leu Pro Phe Glu Lys Ile As - #n Glu Gly Phe Asp     #               360     - Leu Leu Arg Ser Gly Lys Ser Ile Arg Thr Il - #e Leu Thr Phe     #               370     - (2) INFORMATION FOR SEQ ID NO:82:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 374     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #3 Column 3FEATURE: Alcohol dehydrogenase, Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #H.              Joernvall,     #M.            Persson,     #J.            Jeffery,               (B) TITLE: Alcohol dehy - #drogenases     #of the National Academy of Sciences,     #     USA               (D) VOLUME: 78     -           (F) PAGES: 4226-4230     -           (G) DATE: 1981     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:82:     - Ser Thr Ala Gly Lys Val Ile Lys Cys Lys Al - #a Ala Val Leu Trp     #               015     - Glu Leu Lys Lys Pro Phe Ser Ile Glu Glu Va - #l Glu Val Ala Pro     #               030     - Pro Lys Ala His Glu Val Arg Ile Lys Met Va - #l Ala Val Gly Ile     #               045     - Cys Gly Thr Asp Asp His Val Val Ser Gly Th - #r Met Val Thr Pro     #               060     - Leu Pro Val Ile Leu Gly His Glu Ala Ala Gl - #y Ile Val Glu Ser     #               075     - Val Gly Glu Gly Val Thr Thr Val Lys Pro Gl - #y Asp Lys Val Ile     #               090     - Pro Leu Ala Ile Pro Gln Cys Gly Lys Cys Ar - #g Ile Cys Lys Asn     #               105     - Pro Glu Ser Asn Tyr Cys Leu Lys Asn Asp Va - #l Ser Asn Pro Gln     #               120     - Gly Thr Leu Gln Asp Gly Thr Ser Arg Phe Th - #r Cys Arg Arg Lys     #               135     - Pro Ile His His Phe Leu Gly Ile Ser Thr Ph - #e Ser Gln Tyr Thr     #               150     - Val Val Asp Glu Asn Ala Val Ala Lys Ile As - #p Ala Ala Ser Pro     #               165     - Leu Glu Lys Val Cys Leu Ile Gly Cys Gly Ph - #e Ser Thr Gly Tyr     #               180     - Gly Ser Ala Val Asn Val Ala Lys Val Thr Pr - #o Gly Ser Thr Cys     #               195     - Ala Val Phe Gly Leu Gly Gly Val Gly Leu Se - #r Ala Ile Met Gly     #               210     - Cys Lys Ala Ala Gly Ala Ala Ala Ala Arg Il - #e Ile Ala Val Asp     #               225     - Ile Asn Lys Asp Lys Phe Ala Lys Glu Leu Gl - #y Ala Thr Glu Cys     #               240     - Ile Asn Pro Gln Asp Tyr Lys Lys Pro Ile Gl - #n Glu Val Leu Lys     #               255     - Glu Met Thr Asp Gly Gly Val Asp Phe Ser Ph - #e Glu Val Ile Gly     #               270     - Arg Leu Asp Thr Met Met Ala Ser Leu Leu Cy - #s Cys His Glu Ala     #               285     - Cys Gly Thr Ser Val Ile Val Gly Val Pro Pr - #o Asp Ser Gln Asn     #               300     - Leu Ser Met Asn Pro Met Leu Leu Leu Thr Gl - #y Arg Thr Trp Lys     #               315     - Gly Ala Ile Phe Gly Gly Phe Lys Ser Lys Gl - #u Cys Val Pro Lys     #               330     - Leu Val Ala Asp Phe Met Ala Lys Lys Phe Se - #r Leu Asp Ala Leu     #               345     - Ile Thr His Val Leu Pro Phe Glu Lys Ile As - #n Glu Gly Phe Asp     #               360     - Leu Leu His Ser Gly Lys Ser Ile Arg Thr Il - #e Leu Met Phe     #               370     - (2) INFORMATION FOR SEQ ID NO:83:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 374     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #3 Column 4FEATURE: Alcohol dehydrogenase, Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #H.              Joernvall,     #M.            Persson,     #J.            Jeffery,               (B) TITLE: Alcohol dehy - #drogenases     #of the National Academy of Sciences,     #     USA               (D) VOLUME: 78     -           (F) PAGES: 4226-4230     -           (G) DATE: 1981     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:83:     - Ser Thr Ala Gly Lys Val Ile Lys Cys Lys Al - #a Ala Val Leu Trp     #               015     - Glu Val Lys Lys Pro Phe Ser Ile Glu Asp Va - #l Glu Val Ala Pro     #               030     - Pro Lys Ala Tyr Glu Val Arg Ile Lys Met Va - #l Ala Val Gly Ile     #               045     - Cys Arg Thr Asp Asp His Val Val Ser Gly As - #n Leu Val Thr Pro     #               060     - Leu Pro Val Ile Leu Gly His Glu Ala Ala Gl - #y Ile Val Glu Ser     #               075     - Val Gly Glu Gly Val Thr Thr Val Lys Pro Gl - #y Asp Lys Val Ile     #               090     - Pro Leu Phe Thr Pro Gln Cys Gly Lys Cys Ar - #g Val Cys Lys Asn     #               105     - Pro Glu Ser Asn Tyr Cys Leu Lys Asn Asp Le - #u Gly Asn Pro Arg     #               120     - Gly Thr Leu Gln Asp Gly Thr Arg Arg Phe Th - #r Cys Arg Gly Lys     #               135     - Pro Ile His His Phe Leu Gly Thr Ser Thr Ph - #e Ser Gln Tyr Thr     #               150     - Val Val Asp Glu Asn Ala Val Ala Lys Ile As - #p Ala Ala Ser Pro     #               165     - Leu Glu Lys Val Cys Leu Ile Gly Cys Gly Ph - #e Ser Thr Gly Tyr     #               180     - Gly Ser Ala Val Asn Val Ala Lys Val Thr Pr - #o Gly Ser Thr Cys     #               195     - Ala Val Phe Gly Leu Gly Gly Val Gly Leu Se - #r Ala Val Met Gly     #               210     - Cys Lys Ala Ala Gly Ala Ala Ala Ala Arg Il - #e Ile Ala Val Asp     #               225     - Ile Asn Lys Asp Lys Phe Ala Lys Glu Leu Gl - #y Ala Thr Glu Cys     #               240     - Ile Asn Pro Gln Asp Tyr Lys Lys Pro Ile Gl - #n Glu Val Leu Lys     #               255     - Glu Met Thr Asp Gly Gly Val Asp Phe Ser Ph - #e Glu Val Ile Gly     #               270     - Arg Leu Asp Thr Met Met Ala Ser Leu Leu Cy - #s Cys His Glu Ala     #               285     - Cys Gly Thr Ser Val Ile Val Gly Val Pro Pr - #o Ala Ser Gln Asn     #               300     - Leu Ser Ile Asn Pro Met Leu Leu Leu Thr Gl - #y Arg Thr Trp Lys     #               315     - Gly Ala Val Leu Gly Gly Phe Lys Ser Lys Gl - #u Gly Ile Pro Lys     #               330     - Leu Val Ala Asp Phe Met Ala Lys Lys Phe Se - #r Leu Asp Ala Leu     #               345     - Ile Thr His Val Leu Pro Phe Glu Lys Ile As - #n Glu Gly Phe Asp     #               360     - Leu Leu His Ser Gly Lys Ser Ile Arg Thr Va - #l Leu Thr Phe     #               370     - (2) INFORMATION FOR SEQ ID NO:84:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 374     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #3 Column 5FEATURE: Alcohol dehydrogenase, Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #H.              Joernvall,     #M.            Persson,     #J.            Jeffery,               (B) TITLE: Alcohol dehy - #drogenases     #of the National Academy of Sciences,     #     USA               (D) VOLUME: 78     -           (F) PAGES: 4226-4230     -           (G) DATE: 1981     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:84:     - Ser Thr Ala Gly Lys Val Ile Lys Cys Lys Al - #a Ala Val Leu Trp     #               015     - Glu Leu Lys Lys Pro Phe Ser Ile Glu Glu Va - #l Glu Val Ala Pro     #               030     - Pro Lys Ala His Glu Val Arg Ile Lys Met Va - #l Ala Ala Gly Ile     #               045     - Cys Arg Ser Asp Glu His Val Val Ser Gly As - #n Leu Val Thr Pro     #               060     - Leu Pro Val Ile Leu Gly His Glu Ala Ala Gl - #y Ile Val Glu Ser     #               075     - Val Gly Glu Gly Val Thr Thr Val Lys Pro Gl - #y Asp Lys Val Ile     #               090     - Pro Leu Phe Thr Pro Gln Cys Gly Lys Cys Ar - #g Ile Cys Lys Asn     #               105     - Pro Glu Ser Asn Tyr Cys Leu Lys Asn Asp Le - #u Gly Asn Pro Arg     #               120     - Gly Thr Leu Gln Asp Gly Thr Arg Arg Phe Th - #r Cys Ser Gly Lys     #               135     - Pro Ile His His Phe Val Gly Val Ser Thr Ph - #e Ser Gln Tyr Thr     #               150     - Val Val Asp Glu Asn Ala Val Ala Lys Ile As - #p Ala Ala Ser Pro     #               165     - Leu Glu Lys Val Cys Leu Ile Gly Cys Gly Ph - #e Ser Thr Gly Tyr     #               180     - Gly Ser Ala Val Lys Val Ala Lys Val Thr Pr - #o Gly Ser Thr Cys     #               195     - Ala Val Phe Gly Leu Gly Gly Val Gly Leu Se - #r Val Val Met Gly     #               210     - Cys Lys Ala Ala Gly Ala Ala Ala Ala Arg Il - #e Ile Ala Val Asp     #               225     - Ile Asn Lys Asp Lys Phe Ala Lys Glu Leu Gl - #y Ala Thr Glu Cys     #               240     - Ile Asn Pro Gln Asp Tyr Lys Lys Pro Ile Gl - #n Glu Val Leu Lys     #               255     - Glu Met Thr Asp Gly Gly Val Asp Phe Ser Ph - #e Glu Val Ile Gly     #               270     - Gln Leu Asp Thr Met Met Ala Ser Leu Leu Cy - #s Cys His Glu Ala     #               285     - Cys Gly Thr Ser Val Ile Val Gly Val Pro Pr - #o Asp Ser Gln Asn     #               300     - Leu Ser Ile Asn Pro Met Leu Leu Leu Thr Gl - #y Arg Thr Trp Lys     #               315     - Gly Ala Ile Tyr Gly Gly Phe Lys Ser Lys Gl - #u Ser Val Pro Lys     #               330     - Leu Val Ala Asp Phe Met Ala Lys Lys Phe Se - #r Leu Asp Ala Leu     #               345     - Ile Thr Asn Ile Leu Pro Phe Glu Lys Ile As - #n Glu Gly Phe Asp     #               360     - Leu Leu Arg Ser Gly Lys Ser Ile Arg Thr Va - #l Leu Thr Phe     #               370     - (2) INFORMATION FOR SEQ ID NO:85:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 374     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: mouse     #3 Column 6FEATURE: Alcohol dehydrogenase, Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #H.              Joernvall,     #M.            Persson,     #J.            Jeffery,               (B) TITLE: Alcohol dehy - #drogenases     #of the National Academy of Sciences,     #     USA               (D) VOLUME: 78     -           (F) PAGES: 4226-4230     -           (G) DATE: 1981     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:85:     - Ser Thr Ala Gly Lys Val Ile Lys Cys Lys Al - #a Ala Val Leu Trp     #               015     - Glu Leu His Lys Pro Phe Thr Ile Glu Asp Il - #e Glu Val Ala Pro     #               030     - Pro Lys Ala His Glu Val Arg Ile Lys Met Va - #l Ala Thr Gly Val     #               045     - Cys Arg Thr Asp Asp His Val Val Ser Gly Th - #r Leu Val Thr Pro     #               060     - Leu Pro Ala Val Leu Gly His Glu Gly Ala Gl - #y Ile Val Glu Ser     #               075     - Val Gly Glu Gly Val Thr Cys Val Lys Pro Gl - #y Asp Lys Val Ile     #               090     - Pro Leu Phe Ser Pro Gln Cys Gly Glu Cys Ar - #g Ile Cys Lys His     #               105     - Pro Glu Ser Asn Phe Cys Ser Arg Ser Asp Le - #u Leu Met Pro Arg     #               120     - Gly Thr Leu Arg Asp Gly Thr Ser Arg Phe Se - #r Cys Lys Gly Lys     #               135     - Pro Ile His His Phe Ile Ser Thr Ser Thr Ph - #e Ser Gln Tyr Thr     #               150     - Val Val Asp Asp Ile Ala Val Ala Lys Ile As - #p Gly Ala Ser Pro     #               165     - Leu Asp Lys Val Cys Leu Ile Gly Cys Gly Ph - #e Ser Thr Gly Tyr     #               180     - Gly Ser Ala Val Lys Val Ala Lys Val Thr Pr - #o Gly Ser Thr Cys     #               195     - Ala Val Phe Gly Leu Gly Gly Val Gly Leu Se - #r Val Ile Met Gly     #               210     - Cys Lys Ala Ala Gly Ala Ala Ala Ala Lys Il - #e Ile Ala Val Asp     #               225     - Ile Asn Lys Asp Lys Phe Ala Lys Glu Leu Gl - #y Ala Thr Asp Cys     #               240     - Ile Asn Pro Gln Asp Tyr Ser Lys Pro Ile Gl - #n Glu Val Leu Gln     #               255     - Glu Met Thr Asp Gly Gly Val Asp Phe Ser Ph - #e Glu Val Ile Gly     #               270     - Arg Leu Asp Thr Met Thr Ser Ala Leu Leu Se - #r Cys His Ala Ala     #               285     - Cys Gly Val Ser Val Val Val Gly Val Pro Pr - #o Asn Ala Gln Asn     #               300     - Leu Ser Met Asn Pro Met Leu Leu Leu Leu Gl - #y Arg Thr Trp Lys     #               315     - Gly Ala Ile Phe Gly Gly Phe Lys Ser Lys As - #p Ser Val Pro Lys     #               330     - Leu Val Ala Asp Phe Met Ala Lys Lys Phe Pr - #o Leu Glu Pro Leu     #               345     - Ile Thr His Val Leu Pro Phe Glu Lys Ile As - #n Glu Ala Phe Asp     #               360     - Leu Leu Arg Ser Gly Lys Ser Ile Arg Thr Va - #l Leu Thr Phe     #               370     - (2) INFORMATION FOR SEQ ID NO:86:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 375     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: rat     #3 Column 7FEATURE: Alcohol dehydrogenase, Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #H.              Joernvall,     #M.            Persson,     #J.            Jeffery,               (B) TITLE: Alcohol dehy - #drogenases     #of the National Academy of Sciences,     #     USA               (D) VOLUME: 78     -           (F) PAGES: 4226-4230     -           (G) DATE: 1981     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:86:     - Ser Thr Ala Gly Lys Val Ile Lys Cys Lys Al - #a Ala Val Leu Trp     #               015     - Glu Pro His Lys Pro Phe Thr Ile Glu Asp Il - #e Glu Val Ala Pro     #               030     - Pro Lys Ala His Glu Val Arg Ile Lys Met Va - #l Ala Thr Gly Val     #               045     - Cys Arg Ser Asp Asp His Ala Val Ser Gly Se - #r Leu Phe Thr Pro     #               060     - Leu Pro Ala Val Leu Gly His Glu Gly Ala Gl - #y Ile Val Glu Ser     #               075     - Ile Gly Glu Gly Val Thr Cys Val Lys Pro Gl - #y Asp Lys Val Ile     #               090     - Pro Leu Phe Ser Pro Gln Cys Gly Lys Cys Ar - #g Ile Cys Lys His     #               105     - Pro Glu Ser Asn Leu Cys Cys Gln Thr Lys As - #n Leu Thr Gln Pro     #               120     - Lys Gly Ala Leu Leu Glu Gly Thr Ser Arg Ph - #e Ser Cys Arg Gly     #               135     - Lys Gln Ile His Asn Phe Leu Ser Thr Ser Th - #r Phe Ser Gln Tyr     #               150     - Thr Val Val Asp Asp Ile Ala Val Ala Lys Il - #e Asp Ala Ala Ala     #               165     - Pro Leu Asp Lys Val Cys Leu Leu Gly Cys Gl - #y Phe Ser Thr Gly     #               180     - Tyr Gly Ser Ala Val Gln Val Ala Lys Val Th - #r Pro Gly Ser Thr     #               195     - Cys Ala Val Phe Gly Leu Gly Gly Val Gly Le - #u Ser Val Val Ile     #               210     - Gly Cys Lys Thr Ala Gly Ala Ala Ala Ala Ar - #g Ile Ile Ala Val     #               225     - Asp Ile Asn Lys Asp Lys Phe Ala Lys Glu Le - #u Gly Ala Thr Glu     #               240     - Cys Ile Asn Pro Gln Asp Tyr Thr Lys Pro Il - #e Gln Glu Val Leu     #               255     - Gln Glu Met Thr Asp Gly Gly Val Asp Phe Se - #r Phe Glu Val Ile     #               270     - Gly Arg Leu Asp Thr Met Thr Ser Ala Leu Le - #u Ser Cys His Ser     #               285     - Ala Cys Gly Val Ser Val Ile Val Gly Val Pr - #o Pro Ser Ala Gln     #               300     - Ser Leu Ser Val Asn Pro Met Ser Leu Leu Le - #u Gly Arg Thr Trp     #               315     - Lys Gly Ala Ile Phe Gly Gly Phe Lys Ser Ly - #s Asp Ala Val Pro     #               330     - Lys Leu Val Ala Asp Phe Met Ala Lys Lys Ph - #e Pro Leu Asp Pro     #               345     - Leu Ile Thr His Val Leu Pro Phe Glu Lys Il - #e Asn Glu Ala Phe     #               360     - Asp Leu Leu Arg Ala Gly Lys Ser Ile Arg Th - #r Val Leu Thr Phe     #               375     - (2) INFORMATION FOR SEQ ID NO:87:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 379     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     #3 Column 8FEATURE: Alcohol dehydrogenase, Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #H.              Joernvall,     #M.            Persson,     #J.            Jeffery,               (B) TITLE: Alcohol dehy - #drogenases     #of the National Academy of Sciences,     #     USA               (D) VOLUME: 78     -           (F) PAGES: 4226-4230     -           (G) DATE: 1981     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:87:     - Gly Thr Lys Gly Lys Val Ile Lys Cys Lys Al - #a Ala Ile Ala Trp     #               015     - Glu Ala Gly Lys Pro Leu Cys Ile Glu Glu Va - #l Glu Val Ala Pro     #               030     - Pro Lys Ala His Glu Val Arg Ile Gln Ile Il - #e Ala Thr Ser Leu     #               045     - Cys His Ser Asp Ala Ser Val Ile Asp Ser Ly - #s Phe Glu Gly Leu     #               060     - Ala Phe Pro Val Ile Val Gly His Glu Ala Al - #a Gly Ile Val Glu     #               075     - Ser Ile Gly Pro Gly Val Thr Asn Val Lys Pr - #o Gly Asp Lys Val     #               090     - Ile Pro Leu Tyr Ala Pro Leu Cys Arg Lys Cy - #s Lys Phe Cys Leu     #               105     - Ser Pro Leu Thr Asn Leu Cys Gly Lys Ile Se - #r Asn Leu Lys Ser     #               120     - Pro Ala Ser Asp Gln Gln Leu Met Glu Asp Ly - #s Thr Ser Arg Phe     #               135     - Thr Cys Lys Gly Lys Pro Val Tyr His Phe Ph - #e Gly Thr Ser Thr     #               150     - Phe Ser Gln Tyr Thr Val Val Ser Asp Ile As - #n Leu Ala Lys Ile     #               165     - Asp Asp Asp Ala Asn Leu Glu Arg Val Cys Le - #u Ile Gly Cys Gly     #               180     - Phe Ser Thr Gly Tyr Gly Ala Ala Ile Asn As - #n Ala Lys Val Thr     #               195     - Pro Gly Ser Thr Cys Ala Val Phe Gly Leu Gl - #y Gly Val Gly Leu     #               210     - Ser Ala Val Ile Gly Cys Lys Ala Ala Gly Al - #a Ser Ala Ser Arg     #               225     - Ile Ile Gly Ile Asp Ile Asn Ser Glu Lys Ph - #e Val Lys Ala Leu     #               240     - Gly Ala Thr Asp Cys Leu Asn Pro Arg Asp Le - #u His Lys Pro Ile     #               255     - Gln Glu Val Ile Ile Glu Leu Thr Lys Gly Gl - #y Val Asp Phe Ala     #               270     - Leu Asp Cys Ala Gly Gly Ser Glu Thr Met Ly - #s Ala Ala Leu Asp     #               285     - Cys Thr Thr Ala Gly Trp Gly Ser Cys Thr Ph - #e Ile Gly Val Ala     #               300     - Ala Gly Ser Lys Gly Leu Thr Ile Phe Pro Gl - #u Glu Leu Ile Ile     #               315     - Gly Arg Thr Ile Asn Gly Thr Phe Phe Gly Gl - #y Trp Lys Ser Val     #               330     - Asp Ser Ile Pro Lys Leu Val Thr Asp Tyr Ly - #s Asn Lys Lys Phe     #               345     - Asn Leu Asp Ala Leu Val Thr His Thr Leu Pr - #o Phe Asp Lys Ile     #               360     - Ser Glu Ala Phe Asp Leu Met Asn Gln Gly Ly - #s Ser Ile Arg Thr     #               375     - Ile Leu Ile Phe     - (2) INFORMATION FOR SEQ ID NO:88:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 376     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: maize     #3 Column 10EATURE: Alcohol dehydrogenase, Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #H.              Joernvall,     #M.            Persson,     #J.            Jeffery,               (B) TITLE: Alcohol dehy - #drogenases     #of the National Academy of Sciences,     #     USA               (D) VOLUME: 78     -           (F) PAGES: 4226-4230     -           (G) DATE: 1981     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:88:     - Ala Thr Ala Gly Lys Val Ile Lys Cys Lys Al - #a Ala Val Ala Trp     #               015     - Glu Ala Gly Lys Pro Leu Ser Ile Glu Glu Va - #l Glu Val Ala Pro     #               030     - Pro Gln Ala Met Glu Val Arg Val Lys Ile Le - #u Phe Thr Ser Leu     #               045     - Cys His Thr Asp Val Tyr Phe Trp Glu Ala Ly - #s Gly Gln Thr Pro     #               060     - Val Phe Pro Arg Ile Phe Gly His Glu Ala Gl - #y Gly Ile Ile Glu     #               075     - Ser Val Gly Glu Gly Val Thr Asp Val Ala Pr - #o Gly Asp His Val     #               090     - Leu Pro Val Phe Thr Gly Glu Cys Lys Glu Cy - #s Ala His Cys Lys     #               105     - Ser Ala Glu Ser Asn Met Cys Asp Leu Leu Ar - #g Ile Asn Thr Asp     #               120     - Arg Gly Val Met Ile Ala Asp Gly Lys Ser Ar - #g Phe Ser Ile Asn     #               135     - Gly Lys Pro Ile Tyr His Phe Val Gly Thr Se - #r Thr Phe Ser Glu     #               150     - Tyr Thr Val Met His Val Gly Cys Val Ala Ly - #s Ile Asn Pro Gln     #               165     - Ala Pro Leu Asp Lys Val Cys Val Leu Ser Cy - #s Gly Ile Ser Thr     #               180     - Gly Leu Gly Ala Ser Ile Asn Val Ala Lys Pr - #o Pro Lys Gly Ser     #               195     - Thr Val Ala Val Phe Gly Leu Gly Ala Val Gl - #y Leu Ala Ala Ala     #               210     - Gly Gly Ala Arg Ile Ala Gly Ala Ser Ala Se - #r Arg Ile Ile Gly     #               225     - Val Asp Leu Asn Pro Ser Arg Phe Glu Arg Ly - #s Phe Gly Cys Thr     #               240     - Glu Phe Val Asn Pro Lys Asp His Asn Lys Pr - #o Val Gln Glu Val     #               255     - Leu Ala Glu Met Thr Asn Gly Gly Val Asp Ar - #g Ser Val Glu Cys     #               270     - Thr Gly Asn Ile Asn Ala Met Ile Gln Ala Ph - #e Glu Cys Val His     #               285     - Asp Gly Trp Gly Val Ala Val Val Val Gly Va - #l Pro His Lys Asp     #               300     - Ala Glu Phe Lys Thr His Pro Met Asn Phe Le - #u Asn Glu Arg Thr     #               315     - Leu Lys Gly Thr Phe Phe Gly Asn Tyr Lys Pr - #o Arg Thr Asp Leu     #               330     - Pro Asn Val Val Glu Leu Tyr Met Lys Lys Gl - #u Leu Glu Val Glu     #               345     - Lys Phe Ile Thr His Ser Val Pro Phe Ala Gl - #u Ile Asn Lys Ala     #               360     - Phe Asp Leu Met Ala Lys Gly Glu Gly Ile Ar - #g Cys Ile Ile Arg     #               375     - Met     - (2) INFORMATION FOR SEQ ID NO:89:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 376     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: maize     #3 Column 11EATURE: Alcohol dehydrogenase, Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #H.              Joernvall,     #M.            Persson,     #J.            Jeffery,               (B) TITLE: Alcohol dehy - #drogenases     #of the National Academy of Sciences,     #     USA               (D) VOLUME: 78     -           (F) PAGES: 4226-4230     -           (G) DATE: 1981     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:89:     - Ala Thr Ala Gly Lys Val Ile Lys Cys Arg Al - #a Ala Val Thr Trp     #               015     - Glu Ala Gly Lys Pro Leu Ser Ile Glu Glu Va - #l Glu Val Ala Pro     #               030     - Pro Gln Ala Met Glu Val Arg Ile Lys Ile Le - #u Tyr Thr Ala Leu     #               045     - Cys His Thr Asp Val Tyr Phe Trp Glu Ala Ly - #s Gly Gln Thr Pro     #               060     - Val Phe Pro Arg Ile Leu Gly His Glu Ala Gl - #y Gly Ile Val Glu     #               075     - Ser Val Gly Glu Gly Val Thr Asp Val Ala Pr - #o Gly Asp His Val     #               090     - Leu Pro Val Phe Thr Gly Glu Cys Lys Glu Cy - #s Ala His Cys Lys     #               105     - Ser Glu Glu Ser Asn Met Cys Asp Leu Leu Ar - #g Ile Asn Val Asp     #               120     - Arg Gly Val Met Ile Gly Asp Gly Lys Ser Ar - #g Phe Thr Ile Ser     #               135     - Gly Gln Pro Ile Phe His Phe Val Gly Thr Se - #r Thr Phe Ser Glu     #               150     - Tyr Thr Val Ile His Val Gly Cys Leu Ala Ly - #s Ile Asn Pro Glu     #               165     - Ala Pro Leu Asp Lys Val Cys Ile Leu Ser Cy - #s Gly Ile Ser Thr     #               180     - Gly Leu Gly Ala Thr Leu Asn Val Ala Lys Pr - #o Ala Lys Gly Ser     #               195     - Thr Val Ala Ile Phe Gly Leu Gly Ala Val Gl - #y Leu Ala Ala Met     #               210     - Glu Gly Ala Arg Leu Ala Gly Ala Ser Ala Se - #r Arg Ile Ile Gly     #               225     - Val Asp Ile Asn Pro Ala Lys Tyr Glu Lys Ly - #s Phe Gly Cys Thr     #               240     - Glu Phe Val Asn Pro Lys Asp His Asp Lys Pr - #o Val Gln Glu Val     #               255     - Leu Ile Glu Leu Thr Asn Gly Gly Val Asp Ar - #g Ser Val Glu Cys     #               270     - Thr Gly Asn Val Asn Ala Met Ile Ser Ala Ph - #e Glu Cys Val His     #               285     - Asp Gly Trp Gly Val Ala Val Val Val Gly Va - #l Pro His Lys Asp     #               300     - Asp Gln Phe Lys Thr His Pro Met Asn Phe Le - #u Ser Glu Lys Thr     #               315     - Leu Lys Gly Thr Phe Phe Gly Asn Tyr Lys Pr - #o Arg Thr Asp Leu     #               330     - Pro Asn Val Val Glu Met Tyr Met Lys Lys Gl - #u Leu Glu Leu Glu     #               345     - Lys Phe Ile Thr His Ser Val Pro Phe Ser Gl - #u Ile Asn Thr Ala     #               360     - Phe Asp Leu Met Leu Lys Gly Glu Gly Leu Ar - #g Cys Ile Met Arg     #               375     - Met     - (2) INFORMATION FOR SEQ ID NO:90:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 377     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: pea     #3 Column 12EATURE: Alcohol dehydrogenase, Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #H.              Joernvall,     #M.            Persson,     #J.            Jeffery,               (B) TITLE: Alcohol dehy - #drogenases     #of the National Academy of Sciences,     #     USA               (D) VOLUME: 78     -           (F) PAGES: 4226-4230     -           (G) DATE: 1981     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:90:     - Ser Asn Thr Val Gly Gln Ile Ile Lys Cys Ar - #g Ala Ala Val Ala     #               015     - Trp Glu Ala Gly Lys Pro Leu Val Ile Glu Gl - #u Val Glu Val Ala     #               030     - Pro Pro Gln Ala Gly Glu Val Arg Leu Lys Il - #e Leu Phe Thr Ser     #               045     - Leu Cys His Thr Asp Val Tyr Phe Trp Glu Al - #a Lys Gly Gln Thr     #               060     - Pro Leu Phe Pro Arg Ile Phe Gly His Glu Al - #a Gly Gly Ile Val     #               075     - Glu Ser Val Gly Glu Gly Val Thr His Leu Ly - #s Pro Gly Asp His     #               090     - Ala Leu Pro Val Phe Thr Gly Glu Cys Gly Gl - #u Cys Pro His Cys     #               105     - Lys Ser Glu Glu Ser Asn Met Cys Asp Leu Le - #u Arg Ile Asn Thr     #               120     - Asp Arg Gly Val Met Leu Asn Asp Asn Lys Se - #r Arg Phe Ser Ile     #               135     - Lys Gly Gln Pro Val His His Phe Val Gly Th - #r Ser Thr Phe Ser     #               150     - Glu Tyr Thr Val Val His Ala Gly Cys Val Al - #a Lys Ile Asn Pro     #               165     - Asp Ala Pro Leu Asp Lys Val Cys Ile Leu Se - #r Cys Gly Ile Cys     #               180     - Thr Gly Leu Gly Ala Thr Ile Asn Val Ala Ly - #s Pro Lys Pro Gly     #               195     - Ser Ser Val Ala Ile Phe Gly Leu Gly Ala Va - #l Gly Leu Ala Ala     #               210     - Ala Glu Gly Ala Arg Ile Ser Gly Ala Ser Al - #a Ser Arg Ile Ile     #               225     - Gly Val Asp Leu Val Ser Ser Arg Phe Glu Ly - #s Lys Phe Gly Val     #               240     - Asn Glu Phe Val Asn Pro Lys Glu His Asp Ly - #s Pro Val Gln Gln     #               255     - Val Ile Ala Glu Met Thr Asn Gly Gly Val As - #p Arg Ala Val Glu     #               270     - Cys Thr Gly Ser Ile Gln Ala Met Ile Ser Al - #a Phe Glu Cys Val     #               285     - His Asp Gly Trp Gly Val Ala Val Val Val Gl - #y Val Pro Ser Lys     #               300     - Asp Asp Ala Phe Lys Thr His Pro Met Asn Ph - #e Leu Asn Glu Arg     #               315     - Thr Leu Lys Gly Thr Phe Tyr Gly Asn Tyr Ly - #s Pro Arg Thr Asp     #               330     - Leu Pro Asn Val Val Glu Lys Tyr Met Lys Gl - #y Glu Leu Glu Leu     #               345     - Glu Lys Phe Ile Thr His Thr Val Pro Phe Se - #r Glu Ile Asn Lys     #               360     - Ala Phe Asp Tyr Met Leu Lys Gly Glu Ser Il - #e Arg Cys Ile Ile     #               375     - Lys Met     - (2) INFORMATION FOR SEQ ID NO:91:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 376     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Arabidopsis     #3 Column 13EATURE: Alcohol dehydrogenase, Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #H.              Joernvall,     #M.            Persson,     #J.            Jeffery,               (B) TITLE: Alcohol dehy - #drogenases     #of the National Academy of Sciences,     #     USA               (D) VOLUME: 78     -           (F) PAGES: 4226-4230     -           (G) DATE: 1981     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:91:     - Ser Thr Thr Gly Gln Ile Ile Arg Cys Lys Al - #a Ala Val Ala Trp     #               015     - Glu Ala Gly Lys Pro Leu Val Ile Glu Glu Va - #l Glu Val Ala Pro     #               030     - Pro Gln Lys His Glu Val Arg Ile Lys Ile Le - #u Phe Thr Ser Leu     #               045     - Cys His Thr Asp Val Tyr Phe Trp Glu Ala Ly - #s Gly Gln Thr Pro     #               060     - Leu Phe Pro Arg Ile Phe Gly His Glu Ala Gl - #y Gly Ile Val Glu     #               075     - Ser Val Gly Glu Gly Val Thr Asp Leu Gln Pr - #o Gly Asp His Val     #               090     - Leu Pro Ile Phe Thr Gly Glu Cys Gly Asp Cy - #s Arg His Cys Gln     #               105     - Ser Glu Glu Ser Asn Met Cys Asp Leu Leu Ar - #g Ile Asn Thr Glu     #               120     - Arg Gly Gly Met Ile His Asp Gly Glu Ser Ar - #g Phe Ser Ile Asn     #               135     - Gly Lys Pro Ile Tyr His Phe Leu Gly Thr Se - #r Thr Phe Ser Glu     #               150     - Tyr Thr Val Val His Ser Gly Gln Val Ala Ly - #s Ile Asn Pro Asp     #               165     - Ala Pro Leu Asp Lys Val Cys Ile Val Ser Cy - #s Gly Leu Ser Thr     #               180     - Gly Leu Gly Ala Thr Leu Asn Val Ala Lys Pr - #o Lys Lys Gly Gln     #               195     - Ser Val Ala Ile Phe Gly Leu Gly Ala Val Gl - #y Leu Gly Ala Ala     #               210     - Glu Gly Ala Arg Ile Ala Gly Ala Ser Ala Se - #r Arg Ile Ile Gly     #               225     - Val Asp Phe Asn Ser Lys Arg Phe Asp Lys Gl - #u Phe Gly Val Thr     #               240     - Glu Cys Val Asn Pro Lys Asp His Asp Lys Pr - #o Ile Gln Gln Val     #               255     - Ile Ala Glu Met Thr Asp Gly Gly Val Asp Ar - #g Ser Val Glu Cys     #               270     - Thr Gly Ser Val Gln Ala Met Ile Gln Ala Ph - #e Glu Cys Val His     #               285     - Asp Gly Trp Gly Val Ala Val Val Val Gly Va - #l Pro Ser Lys Asp     #               300     - Asp Ala Phe Lys Thr His Pro Met Asn Phe Le - #u Asn Glu Arg Thr     #               315     - Leu Lys Gly Thr Phe Phe Gly Asn Tyr Lys Pr - #o Lys Thr Asp Ile     #               330     - Pro Gly Val Val Glu Lys Tyr Met Asn Lys Gl - #u Leu Glu Leu Glu     #               345     - Lys Phe Ile Thr His Thr Val Pro Phe Ser Gl - #u Ile Asn Lys Ala     #               360     - Phe Asp Tyr Met Leu Lys Gly Glu Ser Ile Ar - #g Cys Ile Ile Thr     #               375     - Met     - (2) INFORMATION FOR SEQ ID NO:92:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 344     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Scizosacchar - #omyces pombe     #3 Column 15EATURE: Alcohol dehydrogenase, Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #H.              Joernvall,     #M.            Persson,     #J.            Jeffery,               (B) TITLE: Alcohol dehy - #drogenases     #of the National Academy of Sciences,     #     USA               (D) VOLUME: 78     -           (F) PAGES: 4226-4230     -           (G) DATE: 1981     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:92:     - Ser Ile Pro Glu Thr Gln Lys Gly Val Ile Ph - #e Tyr Glu Ser His     #               015     - Gly Lys Leu Glu Tyr Lys Asp Ile Pro Val Pr - #o Lys Pro Lys Ala     #               030     - Asn Glu Leu Leu Ile Asn Val Lys Tyr Ser Gl - #y Val Cys His Thr     #               045     - Asp Leu His Ala Trp His Gly Asp Trp Pro Le - #u Pro Val Lys Leu     #               060     - Pro Leu Val Gly Gly His Glu Gly Ala Gly Va - #l Val Val Gly Met     #               075     - Gly Glu Asn Val Lys Gly Trp Lys Ile Gly As - #p Tyr Ala Gly Ile     #               090     - Lys Trp Leu Asn Gly Ser Cys Met Ala Cys Gl - #u Tyr Cys Glu Leu     #               105     - Gly Asn Glu Ser Asn Cys Pro His Ala Ser Le - #u Ser Gly Tyr Thr     #               120     - His Asp Gly Ser Phe Gln Gln Tyr Ala Thr Al - #a Asp Ala Val Gln     #               135     - Ala Ala His Ile Pro Gln Gly Thr Asp Leu Al - #a Gln Val Ala Pro     #               150     - Ile Leu Cys Ala Gly Ile Thr Val Tyr Lys Al - #a Leu Lys Ser Ala     #               165     - Asn Leu Met Ala Gly His Trp Val Ala Ile Se - #r Gly Ala Ala Gly     #               180     - Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Ly - #s Ala Met Gly Tyr     #               195     - Tyr Arg Val Leu Gly Ile Asp Gly Gly Glu Gl - #y Lys Glu Glu Arg     #               210     - Ser Ile Gly Gly Glu Val Phe Ile Asp Phe Th - #r Lys Glu Lys Asp     #               225     - Ile Val Gly Ala Val Leu Lys Ala Thr Asp Gl - #y Gly Ala His Gly     #               240     - Val Ile Asn Val Ser Val Ser Glu Ala Ala Il - #e Glu Ala Ser Thr     #               255     - Arg Tyr Val Arg Ala Asn Gly Thr Thr Val Va - #l Val Gly Met Pro     #               270     - Ala Gly Ala Lys Cys Cys Ser Asp Val Phe As - #n Gln Val Val Lys     #               285     - Ser Ile Ser Ile Lys Gly Ser Tyr Val Gly As - #n Arg Ala Asp Thr     #               300     - Arg Glu Ala Leu Asp Phe Phe Ala Arg Gly Le - #u Val Lys Ser Pro     #               315     - Ile Lys Val Val Gly Leu Ser Thr Leu Pro Gl - #u Ile Tyr Glu Lys     #               330     - Met Glu Lys Gly Gln Ile Val Gly Arg Tyr Va - #l Val Asp Thr     #               340     - (2) INFORMATION FOR SEQ ID NO:93:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 344     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Aspergillus     #3 Column 16EATURE: Alcohol dehydrogenase, Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #H.              Joernvall,     #M.            Persson,     #J.            Jeffery,               (B) TITLE: Alcohol dehy - #drogenases     #of the National Academy of Sciences,     #     USA               (D) VOLUME: 78     -           (F) PAGES: 4226-4230     -           (G) DATE: 1981     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:93:     - Ser Ile Pro Glu Thr Gln Lys Ala Ile Ile Ph - #e Tyr Glu Ser Asn     #               015     - Gly Lys Leu Glu His Lys Asp Ile Pro Val Pr - #o Lys Pro Lys Pro     #               030     - Asn Glu Leu Leu Ile Asn Val Lys Tyr Ser Gl - #y Val Cys His Thr     #               045     - Asp Leu His Ala Trp His Gly Asp Trp Pro Le - #u Pro Thr Lys Leu     #               060     - Pro Leu Val Gly Gly His Glu Gly Ala Gly Va - #l Val Val Gly Met     #               075     - Gly Glu Asn Val Lys Gly Trp Lys Ile Gly As - #p Tyr Ala Gly Ile     #               090     - Lys Trp Leu Asn Gly Ser Cys Met Ala Cys Gl - #u Tyr Cys Glu Leu     #               105     - Gly Asn Glu Ser Asn Cys Pro His Ala Asp Le - #u Ser Gly Tyr Thr     #               120     - His Asp Gly Ser Phe Gln Glu Tyr Ala Thr Al - #a Asp Ala Val Gln     #               135     - Ala Ala His Ile Pro Gln Gly Thr Asp Leu Al - #a Glu Val Ala Pro     #               150     - Ile Leu Cys Ala Gly Ile Thr Val Tyr Lys Al - #a Leu Lys Ser Ala     #               165     - Asn Leu Arg Ala Gly His Trp Ala Ala Ile Se - #r Gly Ala Ala Gly     #               180     - Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Ly - #s Ala Met Gly Tyr     #               195     - Tyr Arg Val Leu Gly Ile Asp Gly Gly Pro Gl - #y Lys Glu Glu Thr     #               210     - Ser Leu Gly Gly Glu Val Phe Ile Asp Phe Th - #r Lys Glu Lys Asp     #               225     - Ile Val Ser Ala Val Val Lys Ala Thr Asn Gl - #y Gly Ala His Gly     #               240     - Ile Ile Asn Val Ser Val Ser Glu Ala Ala Il - #e Glu Ala Ser Thr     #               255     - Arg Tyr Cys Arg Ala Asn Gly Thr Val Val Va - #l Val Gly Leu Pro     #               270     - Ala Gly Ala Lys Cys Ser Ser Asp Val Phe As - #n His Val Val Lys     #               285     - Ser Ile Ser Ile Val Gly Ser Tyr Val Gly As - #n Arg Ala Asp Thr     #               300     - Arg Glu Ala Leu Asp Phe Phe Ala Arg Gly Le - #u Val Lys Ser Pro     #               315     - Ile Lys Val Val Gly Leu Ser Ser Leu Pro Gl - #u Ile Tyr Glu Lys     #               330     - Met Glu Lys Gly Gln Ile Ala Gly Arg Tyr Va - #l Val Asp Thr     #               340     - (2) INFORMATION FOR SEQ ID NO:94:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 345     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Sacharomyces - # cerivisiae     #3 Column 17EATURE: Alcohol dehydrogenase, Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #H.              Joernvall,     #M.            Persson,     #J.            Jeffery,               (B) TITLE: Alcohol dehy - #drogenases     #of the National Academy of Sciences,     #     USA               (D) VOLUME: 78     -           (F) PAGES: 4226-4230     -           (G) DATE: 1981     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:94:     - Ala Ala Ile Pro Lys Thr Gln Lys Gly Val Il - #e Phe Tyr Glu Asn     #               015     - Lys Gly Lys Leu His Tyr Lys Asp Ile Pro Va - #l Pro Lys Pro Lys     #               030     - Pro Asn Glu Ile Leu Ile Asn Met Lys Tyr Se - #r Gly Val Cys His     #               045     - Thr Asp Leu His Ala Trp His Gly Asp Trp Pr - #o Leu Pro Val Lys     #               060     - Leu Pro Leu Val Gly Gly His Glu Gly Ala Gl - #y Val Val Val Lys     #               075     - Leu Gly Ser Asn Val Lys Gly Trp Lys Val Gl - #y Asp Leu Ala Gly     #               090     - Ile Lys Trp Leu Asn Gly Ser Cys Met Thr Cy - #s Glu Phe Cys Glu     #               105     - Ser Gly His Glu Ser Asn Cys Pro Asp Ala As - #p Leu Ser Gly Tyr     #               120     - Thr His Asp Gly Ser Phe Gln Gln Phe Ala Th - #r Ala Asp Ala Ile     #               135     - Gln Ala Ala Lys Ile Gln Gln Gly Thr Asp Le - #u Ala Glu Val Ala     #               150     - Pro Ile Leu Cys Ala Gly Val Thr Val Tyr Ly - #s Ala Leu Lys Glu     #               165     - Ala Asp Leu Lys Ala Gly Asp Trp Val Ala Il - #e Ser Gly Ala Ala     #               180     - Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Al - #a Thr Ala Met Gly     #               195     - Tyr Tyr Arg Val Leu Gly Ile Asp Ala Gly Gl - #u Glu Lys Glu Lys     #               210     - Lys Lys Leu Gly Gly Glu Val Phe Ile Asp Ph - #e Thr Lys Thr Lys     #               225     - Asn Met Val Ser Asp Ile Gln Glu Ala Thr Ly - #s Gly Gly Pro His     #               240     - Gly Val Ile Asn Val Ser Val Ser Glu Ala Al - #a Ile Ser Leu Ser     #               255     - Thr Glu Tyr Val Arg Pro Cys Gly Thr Val Va - #l Val Val Gly Leu     #               270     - Pro Ala Asn Ala Tyr Val Lys Ser Glu Val Ph - #e Ser His Val Val     #               285     - Lys Ser Ile Asn Ile Val Gly Ser Tyr Val Gl - #y Asn Arg Ala Asp     #               300     - Thr Arg Glu Ala Leu Asp Phe Phe Ser Arg Gl - #y Leu Ile Lys Ser     #               315     - Pro Ile Lys Ile Val Gly Leu Ser Glu Leu Pr - #o Lys Val Tyr Asp     #               330     - Leu Met Glu Lys Gly Lys Ile Leu Gly Arg Ty - #r Val Val Asp Thr     #               345     - (2) INFORMATION FOR SEQ ID NO:95:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 349     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Sacharomyces - # cerivisiae     #3 Column 18EATURE: Alcohol dehydrogenase, Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #H.              Joernvall,     #M.            Persson,     #J.            Jeffery,               (B) TITLE: Alcohol dehy - #drogenases     #of the National Academy of Sciences,     #     USA               (D) VOLUME: 78     -           (F) PAGES: 4226-4230     -           (G) DATE: 1981     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:95:     - Met Ser Val Pro Glu Val Gln Trp Ala Gln Va - #l Val Glu Lys Ala     #               015     - Gly Thr Pro Pro Val Tyr Lys Gln Val Pro Va - #l Pro Glu Pro Gly     #               030     - Pro Asp Glu Ile Leu Val Lys Ile Arg Tyr Se - #r Gly Val Cys His     #               045     - Thr Asp Leu His Ala Met Lys Gly Asp Trp Pr - #o Leu Pro Ser Lys     #               060     - Met Pro Leu Ile Gly Gly His Glu Gly Ala Gl - #y Val Val Val Ala     #               075     - Lys Gly Glu Leu Val Lys Asp Glu Asp Phe Ly - #s Ile Gly Asp Arg     #               090     - Ala Gly Ile Lys Trp Leu Asn Gly Ser Cys Le - #u Ser Cys Glu Met     #               105     - Cys Met Gln Ala Asp Glu Pro Leu Cys Pro Hi - #s Ala Asp Leu Ser     #               120     - Gly Tyr Thr Val Asp Gly Thr Phe Gln Gln Ty - #r Thr Ile Gly Lys     #               135     - Ala Ala Leu Ala Ser Lys Ile Pro Asp Asn Va - #l Pro Leu Asp Ala     #               150     - Ala Ala Pro Ile Leu Cys Ala Gly Ile Thr Va - #l Tyr Lys Gly Leu     #               165     - Lys Glu Ser Gly Ala Arg Pro Gly Gln Thr Va - #l Ala Ile Val Gly     #               180     - Ala Gly Gly Gly Leu Gly Ser Leu Ala Gln Gl - #n Tyr Ala Lys Ala     #               195     - Met Gly Leu Leu Arg Thr Ile Ala Ile Asp Se - #r Gly Asp Glu Lys     #               210     - Lys Ala Glu Gln Leu Gly Ala Glu Val Phe Il - #e Asp Phe Ser Lys     #               225     - Ser Ala Asp Val Val Ala Asp Val Lys Ala Al - #a Thr Pro Gly Gly     #               240     - Leu Gly Ala His Ala Val Ile Leu Leu Ala Va - #l Ala Glu Lys Pro     #               255     - Phe Gln Gln Ala Thr Glu Tyr Val Arg Ser Hi - #s Gly Ser Val Val     #               270     - Ile Ile Gly Leu Pro Ala Asn Ala Phe Leu Ly - #s Ala Pro Val Phe     #               285     - Thr Thr Val Val Arg Met Ile Asn Ile Lys Gl - #y Ser Tyr Val Gly     #               300     - Asn Arg Gln Asp Gly Val Glu Ala Leu Asp Ph - #e Phe Ala Arg Gly     #               315     - Leu Ile Lys Ala Pro Phe Lys Lys Ala Pro Le - #u Gln Asp Leu Pro     #               330     - Gln Ile Phe Glu Leu Met Gly Gln Gly Lys Il - #e Ala Gly Arg Tyr     #               345     - Val Leu Glu Ile     - (2) INFORMATION FOR SEQ ID NO:96:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 346     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Sacharomyces - # cerivisiae     #3 Column 19EATURE: Alcohol dehydrogenase, Table     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #H.              Joernvall,     #M.            Persson,     #J.            Jeffery,               (B) TITLE: Alcohol dehy - #drogenases     #of the National Academy of Sciences,     #     USA               (D) VOLUME: 78     -           (F) PAGES: 4226-4230     -           (G) DATE: 1981     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:96:     - Thr Ile Pro Asp Lys Gln Leu Ala Ala Val Ph - #e His Thr His Gly     #               015     - Gly Pro Glu Asn Val Lys Phe Glu Glu Val Pr - #o Val Ala Glu Pro     #               030     - Gly Gln Asp Glu Val Leu Val Val Asn Lys Ty - #r Thr Gly Val Cys     #               045     - His Thr Asp Leu His Ala Leu Gln Gly Asp Tr - #p Pro Leu Pro Ala     #               060     - Lys Met Pro Leu Ile Gly Gly His Glu Gly Al - #a Gly Val Val Val     #               075     - Lys Val Gly Ala Gly Val Thr Arg Leu Lys Il - #e Gly Asp Arg Val     #               090     - Gly Val Lys Trp Met Asn Ser Ser Cys Gly As - #n Cys Glu Tyr Cys     #               105     - Met Lys Ala Glu Glu Thr Ile Cys Pro His Il - #e Gln Leu Ser Gly     #               120     - Tyr Thr Val Asp Gly Thr Phe Gln His Tyr Cy - #s Ile Ala Asn Ala     #               135     - Thr His Ala Thr Ile Ile Pro Glu Ser Val Pr - #o Leu Glu Val Ala     #               150     - Ala Pro Ile Met Cys Ala Gly Ile Thr Cys Ty - #r Arg Ala Leu Lys     #               165     - Glu Ser Lys Val Gly Pro Gly Glu Trp Ile Cy - #s Ile Pro Gly Ala     #               180     - Gly Gly Gly Leu Gly His Leu Ala Val Gln Ty - #r Ala Lys Ala Met     #               195     - Ala Met Met Arg Val Val Ala Ile Asp Thr Gl - #y Asp Asp Lys Ala     #               210     - Glu Lys Ser Phe Gly Ala Glu Val Phe Leu As - #p Phe Lys Lys Glu     #               225     - Ala Asp Met Ile Glu Ala Val Lys Ala Cys Th - #r Asn Gly Gly Ala     #               240     - His Gly Thr Leu Val Leu Ser Thr Ser Pro Ly - #s Ser Tyr Glu Gln     #               255     - Ala Ala Gly Phe Ala Arg Pro Gly Ser Thr Me - #t Val Val Val Ser     #               270     - Met Pro Ala Gly Ala Lys Leu Gly Ala Asp Il - #e Phe Trp Leu Thr     #               285     - Val Lys Met Leu Lys Ile Cys Gly Ser His Va - #l Gly Asn Arg Ile     #               300     - Asp Ser Ile Glu Ala Leu Glu Tyr Val Ser Ar - #g Gly Leu Val Lys     #               315     - Pro Tyr Tyr Lys Val Gln Pro Phe Ser Thr Le - #u Pro Asp Val Tyr     #               330     - Arg Leu Met His Glu Asn Lys Ile Ala Gly Ar - #g Ile Val Leu Asp     #               345     - Leu     - (2) INFORMATION FOR SEQ ID NO:97:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 135     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: tobacco     #Table 16 Row 1URE: Pathogenesis related protein,     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #R.              Cutt, J.                    Dixon, D. - # C.     #P.            Carr, J.     #D. F.         Klessig,               (B) TITLE: Isolation an - #d nucleotide sequence of cDNA     clones     #   for the pathogenesis related proteins of     #   Nicotiniana tabacum induced by TMV infection - #.               (C) JOURNAL: Nucleic Ac - #ids Research     -           (D) VOLUME: 16     -           (F) PAGES: 9861-     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:97:     - Gln Asn Ser Pro Gln Asp Tyr Leu Ala Val Hi - #s Asn Asp Ala Arg     #               015     - Ala Gln Val Gly Val Gly Pro Met Ser Trp As - #p Ala Asn Leu Ala     #               030     - Ser Arg Ala Gln Asn Tyr Ala Asn Ser Arg Al - #a Gly Asp Cys Asn     #               045     - Leu Ile His Ser Gly Ala Gly Glu Asn Leu Al - #a Lys Gly Gly Gly     #               060     - Asp Phe Thr Gly Arg Ala Ala Val Gln Leu Tr - #p Val Ser Glu Arg     #               075     - Pro Ser Tyr Asn Tyr Ala Thr Asn Gln Cys Va - #l Gly Gly Lys Lys     #               090     - Cys Arg His Tyr Thr Gln Val Val Trp Arg As - #n Ser Val Arg Leu     #               105     - Gly Cys Gly Arg Ala Arg Cys Asn Asn Gly Tr - #p Trp Phe Ile Ser     #               120     - Cys Asn Tyr Asp Pro Val Gly Asn Trp Leu Gl - #y Gln Arg Pro Tyr     #               135     - (2) INFORMATION FOR SEQ ID NO:98:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 135     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Lycopersicon - # esculentum     #Table 16 Row 2URE: Pathogenesis related protein,     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #R.              Cutt, J.                    Dixon, D. - # C.     #P.            Carr, J.     #D. F.         Klessig,               (B) TITLE: Isolation an - #d nucleotide sequence of cDNA     clones     #   for the pathogenesis related proteins of     #   Nicotiniana tabacum induced by TMV infection - #.               (C) JOURNAL: Nucleic Ac - #ids Research     -           (D) VOLUME: 16     -           (F) PAGES: 9861     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:98:     - Gln Asn Ser Pro Gln Asp Tyr Leu Ala Val Hi - #s Asn Asp Ala Arg     #               015     - Ala Gln Val Gly Val Gly Pro Met Ser Trp As - #p Ala Asn Leu Ala     #               030     - Ser Arg Ala Gln Asn Tyr Ala Asn Ser Arg Al - #a Gly Asp Cys Asn     #               045     - Leu Ile His Ser Gly Ala Gly Glu Asn Leu Al - #a Lys Gly Gly Gly     #               060     - Asp Phe Thr Gly Arg Ala Ala Val Gln Leu Tr - #p Val Ser Glu Arg     #               075     - Pro Ser Tyr Asn Tyr Ala Thr Asn Gln Cys Va - #l Gly Gly Lys Lys     #               090     - Cys Arg His Tyr Thr Gln Val Val Trp Arg As - #n Ser Val Arg Leu     #               105     - Gly Cys Gly Arg Ala Arg Cys Asn Asn Gly Tr - #p Trp Phe Ile Ser     #               120     - Cys Asn Tyr Asp Pro Val Gly Asn Trp Ile Gl - #y Gln Arg Pro Tyr     #               135     - (2) INFORMATION FOR SEQ ID NO:99:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 135     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Lycopersicon - # esculentum     #Table 16 Row 3URE: Pathogenesis related protein;     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #R.              Cutt, J.                    Dixon, D. - # C.     #P.            Carr, J.     #D. F.         Klessig,               (B) TITLE: Isolation an - #d nucleotide sequence of cDNA     clones     #   for the pathogenesis related proteins of     #   Nicotiniana tabacum induced by TMV infection - #.               (C) JOURNAL: Nucleic Ac - #ids Research     -           (D) VOLUME: 16     -           (F) PAGES: 9861     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:99:     - Gln Asn Ser Pro Gln Asp Tyr Leu Ala Val Hi - #s Asn Asp Ala Arg     #               015     - Ala Gln Val Gly Val Gly Pro Met Ser Trp As - #p Ala Asn Leu Ala     #               030     - Ser Arg Ala Gln Asn Tyr Ala Asn Ser Arg Al - #a Gly Asp Cys Asn     #               045     - Leu Ile His Ser Gly Ala Gly Glu Asn Leu Al - #a Lys Gly Gly Gly     #               060     - Asp Phe Thr Gly Arg Ala Ala Val Gln Leu Tr - #p Val Ser Glu Arg     #               075     - Pro Asp Tyr Asn Tyr Ala Thr Asn Gln Cys Va - #l Gly Gly Lys Met     #               090     - Cys Gly His Tyr Thr Gln Val Val Trp Arg As - #n Ser Val Arg Leu     #               105     - Gly Cys Gly Arg Ala Arg Cys Asn Asn Gly Tr - #p Trp Phe Ile Ser     #               120     - Cys Asn Tyr Asp Pro Val Gly Asn Trp Val Gl - #y Glu Arg Pro Tyr     #               135     - (2) INFORMATION FOR SEQ ID NO:100:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 138     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: tobacco     #Table 16 Row 4URE: Pathogenesis related protein;     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #R.              Cutt, J.                    Dixon, D. - # C.     #P.            Carr, J.     #D. F.         Klessig,               (B) TITLE: Isolation an - #d nucleotide sequence of cDNA     clones     #   for the pathogenesis related proteins of     #   Nicotiniana tabacum induced by TMV infection - #.               (C) JOURNAL: Nucleic Ac - #ids Research     -           (D) VOLUME: 16     -           (F) PAGES: 9861     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:100:     - Gln Asn Ser Gln Gln Asp Tyr Leu Asp Ala Hi - #s Asn Thr Ala Arg     #               015     - Ala Asp Val Gly Val Glu Pro Leu Thr Trp As - #p Asp Gln Val Ala     #               030     - Ala Tyr Ala Gln Asn Tyr Ala Ser Gln Leu Al - #a Ala Asp Cys Asn     #               045     - Leu Val His Ser His Gly Gln Tyr Gly Glu As - #n Leu Ala Trp Gly     #               060     - Ser Gly Asp Phe Leu Thr Ala Ala Lys Ala Va - #l Glu Met Trp Val     #               075     - Asn Glu Lys Gln Tyr Tyr Ala His Asp Ser As - #n Thr Cys Ala Gln     #               090     - Gly Gln Val Cys Gly His Tyr Thr Gln Val Va - #l Trp Arg Asn Ser     #               105     - Val Arg Val Gly Cys Ala Arg Val Gln Cys As - #n Asn Gly Gly Tyr     #               120     - Ile Val Ser Cys Asn Tyr Asp Pro Pro Gly As - #n Val Ile Gly Lys     #               135     - Ser Pro Tyr                     140     - (2) INFORMATION FOR SEQ ID NO:101:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 139     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: tobacco     #Table 16 Row 5URE: Pathogenesis related protein;     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #R.              Cutt, J.                    Dixon, D. - # C.     #P.            Carr, J.     #D. F.         Klessig,               (B) TITLE: Isolation an - #d nucleotide sequence of cDNA     clones     #   for the pathogenesis related proteins of     #   Nicotiniana tabacum induced by TMV infection - #.               (C) JOURNAL: Nucleic Ac - #ids Research     -           (D) VOLUME: 16     -           (F) PAGES: 9861     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:101:     - Ala Gln Asn Ser Gln Gln Asp Tyr Leu Asp Al - #a His Asn Thr Ala     #               015     - Arg Ala Asp Val Gly Val Glu Pro Leu Thr Tr - #p Asp Asp Gln Val     #               030     - Ala Ala Tyr Ala Gln Asn Tyr Ala Ser Gln Le - #u Ala Ala Asp Cys     #               045     - Asn Leu Val His Ser His Gly Gln Tyr Gly Gl - #u Asn Leu Ala Glu     #               060     - Gly Ser Gly Asp Phe Met Thr Ala Ala Lys Al - #a Val Glu Met Trp     #               075     - Val Asp Glu Lys Gln Tyr Tyr Asp His Asp Se - #r Asn Thr Cys Ala     #               090     - Gln Gly Gln Val Cys Gly His Tyr Thr Gln Va - #l Val Trp Arg Asn     #               105     - Ser Val Arg Val Gly Cys Ala Arg Val Gln Cy - #s Asn Asn Gly Gly     #               120     - Tyr Val Val Ser Cys Asn Tyr Asp Pro Pro Gl - #y Asn Tyr Arg Gly     #               135     - Glu Ser Pro Tyr     #               150     - (2) INFORMATION FOR SEQ ID NO:102:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 139     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: tobacco     #Table 16 Row 6URE: Pathogenesis related protein;     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #R.              Cutt, J.                    Dixon, D. - # C.     #P.            Carr, J.     #D. F.         Klessig,               (B) TITLE: Isolation an - #d nucleotide sequence of cDNA     clones     #   for the pathogenesis related proteins of     #   Nicotiniana tabacum induced by TMV infection - #.               (C) JOURNAL: Nucleic Ac - #ids Research     -           (D) VOLUME: 16     -           (F) PAGES: 9861     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:102:     - Ala Gln Asn Ser Gln Gln Asp Tyr Leu Asp Al - #a His Asn Thr Ala     #               015     - Arg Ala Asp Val Gly Val Glu Pro Leu Thr Tr - #p Asp Asn Gly Val     #               030     - Ala Ala Tyr Ala Gln Asn Tyr Val Ser Gln Le - #u Ala Ala Asp Cys     #               045     - Asn Leu Val His Ser His Gly Gln Tyr Gly Gl - #u Asn Leu Ala Gln     #               060     - Gly Ser Gly Asp Phe Met Thr Ala Ala Lys Al - #a Val Glu Met Trp     #               075     - Val Asp Glu Lys Gln Tyr Tyr Asp His Asp Se - #r Asn Thr Cys Ala     #               090     - Gln Gly Gln Val Cys Gly His Tyr Thr Gln Va - #l Val Trp Arg Asn     #               105     - Ser Val Arg Val Gly Cys Ala Arg Val Lys Cy - #s Asn Asn Gly Gly     #               120     - Tyr Val Val Ser Cys Asn Tyr Asp Pro Pro Gl - #y Asn Val Ile Gly     #               135     - Gln Ser Pro Tyr     #               150     - (2) INFORMATION FOR SEQ ID NO:103:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 135     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:     #thaliana (A) ORGANISM: Arabidopsis     #Table 16 Row 7URE: Pathogenesis related protein;     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #R.              Cutt, J.                    Dixon, D. - # C.     #P.            Carr, J.     #D. F.         Klessig,               (B) TITLE: Isolation an - #d nucleotide sequence of cDNA     clones     #   for the pathogenesis related proteins of     #   Nicotiniana tabacum induced by TMV infection - #.               (C) JOURNAL: Nucleic Ac - #ids Research     -           (D) VOLUME: 16     -           (F) PAGES: 9861     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:103:     - Pro Gln Glu Thr Leu Val Val His Asn Lys Al - #a Arg Ala Met Val     #               015     - Gly Val Gly Pro Met Val Trp Asn Glu Thr Le - #u Ala Thr Tyr Ala     #               030     - Gln Ser Tyr Ala His Glu Arg Ala Arg Asp Cy - #s Ala Met Lys His     #               045     - Ser Leu Gly Pro Phe Gly Glu Asn Leu Ala Al - #a Gly Trp Gly Thr     #               060     - Met Ser Gly Pro Val Ala Thr Glu Tyr Trp Me - #t Thr Glu Lys Glu     #               075     - Asn Tyr Asp Tyr Asp Ser Asn Thr Cys Gly Gl - #y Asp Gly Val Cys     #               090     - Gly His Tyr Thr Gln Ile Val Trp Arg Asp Se - #r Val Arg Leu Gly     #               105     - Cys Ala Ser Val Arg Cys Lys Asn Asp Glu Ty - #r Ile Trp Val Ile     #               120     - Cys Ser Tyr Asp Pro Pro Gly Asn Tyr Ile Gl - #y Gln Arg Pro Tyr     #               135     - (2) INFORMATION FOR SEQ ID NO:104:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 141     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: maize     #Table 16 Row 8URE: Pathogenesis related protein;     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #R.              Cutt, J.                    Dixon, D. - # C.     #P.            Carr, J.     #D. F.         Klessig,               (B) TITLE: Isolation an - #d nucleotide sequence of cDNA     clones     #   for the pathogenesis related proteins of     #   Nicotiniana tabacum induced by TMV infection - #.               (C) JOURNAL: Nucleic Ac - #ids Research     -           (D) VOLUME: 16     -           (F) PAGES: 9861     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:104:     - Ser Glu Asn Ser Pro Gln Asp Tyr Leu Thr Pr - #o Gln Asn Ser Ala     #               015     - Arg Ala Ala Val Gly Val Gly Pro Val Thr Tr - #p Ser Thr Lys Leu     #               030     - Gln Gln Phe Ala Glu Lys Tyr Ala Ala Gln Ar - #g Ala Gly Asp Cys     #               045     - Arg Leu Gln His Ser Gly Gly Pro Tyr Gly Gl - #u Asn Ile Phe Trp     #               060     - Gly Ser Ala Gly Phe Asp Trp Lys Ala Val As - #p Ala Val Arg Ser     #               075     - Trp Val Asp Glu Lys Gln Trp Tyr Asn Tyr Al - #a Thr Asn Ser Cys     #               090     - Ala Ala Gly Lys Val Cys Gly His Tyr Thr Gl - #n Val Val Trp Arg     #               105     - Ala Thr Thr Ser Ile Gly Cys Ala Arg Val Va - #l Cys Arg Asp Asn     #               120     - Arg Gly Val Phe Ile Ile Cys Asn Tyr Glu Pr - #o Arg Gly Asn Ile     #               135     - Ala Gly Met Lys Pro Tyr     #               150     - (2) INFORMATION FOR SEQ ID NO:105:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 111     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: tobacco     #Table 17 Row 1URE: Pathogenesis related protein;     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #R.              Cutt, J.                    Dixon, D. - # C.     #P.            Carr, J.     #D. F.         Klessig,               (B) TITLE: Isolation an - #d nucleotide sequence of cDNA     clones     #   for the pathogenesis related proteins of     #   Nicotiniana tabacum induced by TMV infection - #.               (C) JOURNAL: Nucleic Ac - #ids Research     -           (D) VOLUME: 16     -           (F) PAGES: 9861     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:105:     - Met Ser Trp Asp Ala Asn Leu Ala Ser Arg Al - #a Gln Asn Tyr Ala     #               015     - Asn Ser Arg Ala Gly Asp Cys Asn Leu Ile Hi - #s Ser Gly Ala Gly     #               030     - Glu Asn Leu Ala Lys Gly Gly Gly Asp Phe Th - #r Gly Arg Ala Ala     #               045     - Val Gln Leu Trp Val Ser Glu Arg Pro Ser Ty - #r Asn Tyr Ala Thr     #               060     - Asn Gln Cys Val Gly Gly Lys Lys Cys Arg Hi - #s Tyr Thr Gln Val     #               075     - Val Trp Arg Asn Ser Val Arg Leu Gly Cys Gl - #y Arg Ala Arg Cys     #               090     - Asn Asn Gly Trp Trp Phe Ile Ser Cys Asn Ty - #r Asp Pro Val Gly     #               105     - Asn Trp Ile Gly Gln Arg     #               120     - (2) INFORMATION FOR SEQ ID NO:106:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 114     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: tobacco     #1a; Table 17 Row 2 Pathogenesis related protein     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #R.              Cutt, J.                    Dixon, D. - # C.     #P.            Carr, J.     #D. F.         Klessig,               (B) TITLE: Isolation an - #d nucleotide sequence of cDNA     clones     #   for the pathogenesis related proteins of     #   Nicotiniana tabacum induced by TMV infection - #.               (C) JOURNAL: Nucleic Ac - #ids Research     -           (D) VOLUME: 16     -           (F) PAGES: 9861     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:106:     - Leu Thr Trp Asp Asp Gln Val Ala Ala Tyr Al - #a Gln Asn Tyr Ala     #               015     - Ser Gln Leu Ala Ala Asp Cys Asn Leu Val Hi - #s Ser His Gly Gln     #               030     - Tyr Gly Glu Asn Leu Ala Glu Gly Ser Gly As - #p Phe Met Thr Ala     #               045     - Ala Lys Ala Val Glu Met Trp Val Asp Glu Ly - #s Gln Tyr Tyr Asp     #               060     - His Asp Ser Asn Thr Cys Ala Gln Gly Gln Va - #l Cys Gly His Tyr     #               075     - Thr Gln Val Val Trp Arg Asn Ser Val Arg Va - #l Gly Cys Ala Arg     #               090     - Val Gln Cys Asn Asn Gly Gly Tyr Val Val Se - #r Cys Asn Tyr Asp     #               105     - Pro Pro Gly Asn Tyr Arg Gly Glu Ser                     110     - (2) INFORMATION FOR SEQ ID NO:107:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 114     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: tobacco     #1b; Table 17 Row 3 Pathogenesis related protein     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #R.              Cutt, J.                    Dixon, D. - # C.     #P.            Carr, J.     #D. F.         Klessig,               (B) TITLE: Isolation an - #d nucleotide sequence of cDNA     clones     #   for the pathogenesis related proteins of     #   Nicotiniana tabacum induced by TMV infection - #.               (C) JOURNAL: Nucleic Ac - #ids Research     -           (D) VOLUME: 16     -           (F) PAGES: 9861     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:107:     - Leu Thr Trp Asp Asn Gly Val Ala Ala Tyr Al - #a Gln Asn Tyr Val     #               015     - Ser Gln Leu Ala Ala Asp Cys Asn Leu Val Hi - #s Ser His Gly Gln     #               030     - Tyr Gly Glu Asn Leu Ala Gln Gly Ser Gly As - #p Phe Met Thr Ala     #               045     - Ala Lys Ala Val Glu Met Trp Val Asp Glu Ly - #s Gln Tyr Tyr Asp     #               060     - His Asp Ser Asn Thr Cys Ala Gln Gly Gln Va - #l Cys Gly His Tyr     #               075     - Thr Gln Val Val Trp Arg Asn Ser Val Arg Va - #l Gly Cys Ala Arg     #               090     - Val Lys Cys Asn Asn Gly Gly Tyr Val Val Se - #r Cys Asn Tyr Asp     #               105     - Pro Pro Gly Asn Val Ile Gly Gln Ser                     110     - (2) INFORMATION FOR SEQ ID NO:108:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 114     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: tobacco     #1c; Table 17 Row 4 Pathogenesis related protein     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #R.              Cutt, J.                    Dixon, D. - # C.     #P.            Carr, J.     #D. F.         Klessig,               (B) TITLE: Isolation an - #d nucleotide sequence of cDNA     clones     #   for the pathogenesis related proteins of     #   Nicotiniana tabacum induced by TMV infection - #.               (C) JOURNAL: Nucleic Ac - #ids Research     -           (D) VOLUME: 16     -           (F) PAGES: 9861     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:108:     - Leu Thr Trp Asp Asp Gln Val Ala Ala Tyr Al - #a Gln Asn Tyr Ala     #               015     - Ser Gln Leu Ala Ala Asp Cys Asn Leu Val Hi - #s Ser His Gly Gln     #               030     - Tyr Gly Glu Asn Leu Ala Trp Gly Ser Gly As - #p Phe Leu Thr Ala     #               045     - Ala Lys Ala Val Glu Met Trp Val Asn Glu Ly - #s Gln Tyr Tyr Ala     #               060     - His Asp Ser Asn Thr Cys Ala Gln Gly Gln Va - #l Cys Gly His Tyr     #               075     - Thr Gln Val Val Trp Arg Asn Ser Val Arg Va - #l Gly Cys Ala Arg     #               090     - Val Gln Cys Asn Asn Gly Gly Tyr Ile Val Se - #r Cys Asn Tyr Asp     #               105     - Pro Pro Gly Asn Val Ile Gly Lys Ser     #               120     - (2) INFORMATION FOR SEQ ID NO:109:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 112     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: tobacco     #basic form;EATURE: Pathogenesis related protein,     #Table 17 Row 5     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #R.              Cutt, J.                    Dixon, D. - # C.     #P.            Carr, J.     #D. F.         Klessig,               (B) TITLE: Isolation an - #d nucleotide sequence of cDNA     clones     #   for the pathogenesis related proteins of     #   Nicotiniana tabacum induced by TMV infection - #.               (C) JOURNAL: Nucleic Ac - #ids Research     -           (D) VOLUME: 16     -           (F) PAGES: 9861     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:109:     - Met Thr Trp Asp Asn Arg Leu Ala Ala Tyr Al - #a Gln Asn Tyr Ala     #               015     - Asn Gln Arg Ile Gly Asp Cys Gly Met Ile Hi - #s Ser His Gly Pro     #               030     - Tyr Gly Glu Asn Leu Ala Ala Ala Phe Pro Gl - #n Leu Asn Ala Ala     #               045     - Gly Ala Val Lys Met Trp Val Asp Glu Lys Ar - #g Phe Tyr Asp Tyr     #               060     - Asn Ser Asn Ser Cys Val Gly Gly Val Cys Gl - #y His Tyr Thr Gln     #               075     - Val Val Trp Arg Asn Ser Val Arg Leu Gly Cy - #s Ala Arg Val Arg     #               090     - Ser Asn Asn Gly Trp Phe Phe Ile Thr Cys As - #n Tyr Asp Pro Pro     #               105     - Gly Asn Phe Ile Gly Gln Arg     #               120     - (2) INFORMATION FOR SEQ ID NO:110:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 137     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: rat     #Table 17 Row 6URE: sperm coating glycoprotein;     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     -                Brooks, D - #. E.                    Means, A. - # R.                    Wright, E - #. J.                    Singh, S. - # P.                    Tiver, K. - # K.               (B) TITLE: Molecular cl - #oning of the cDNA for androgen-     #by dependent sperm coating glycoprotein secreted     #   the rat epidemis.               (C) JOURNAL: European J - #ournal of Biochemistry     -           (D) VOLUME: 161     -           (F) PAGES: 13-18     -           (G) DATE: 1986     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:110:     - Leu Arg Val Glu Trp Asp His Asp Ala Tyr Va - #l Asn Ala Gln Lys     #               015     - Trp Ala Asn Arg Cys Ile Tyr Asn His Ser Pr - #o Leu Gln His Arg     #               030     - Thr Thr Thr Leu Lys Cys Gly Glu Asn Leu Ph - #e Met Ala Asn Tyr     #               045     - Pro Ala Ser Trp Ser Ser Val Ile Gln Asp Tr - #p Tyr Asp Glu Ser     #               060     - Leu Asp Phe Val Phe Gly Phe Gly Pro Lys Ly - #s Val Gly Val Lys     #               075     - Val Gly His Tyr Thr Gln Val Val Trp Asn Se - #r Thr Phe Leu Val     #               090     - Ala Cys Gly Val Ala Glu Cys Pro Asp Gln Pr - #o Leu Lys Tyr Phe     #               105     - Tyr Val Cys His Tyr Cys Pro Gly Gly Asn Ty - #r Val Gly Arg Leu     #               120     - Tyr Ser Pro Tyr Thr Glu Gly Glu Pro Cys As - #p Ser Cys Pro Gly     #               135     - Asn Cys     - (2) INFORMATION FOR SEQ ID NO:111:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 137     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     -     (ix) FEATURE: testis specific protein; Ta - #ble 17 Row 7     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #M.              Kasahara,     #J.            Gutknecht,                    Brew, K.                    Spurr, N.     #P. N.         Goodfellow,     #mapping of a testis-specific gene with     #   sequence similarity to a sperm coating gl - #ycoprotein     #   gene               (C) JOURNAL: Genomics     -           (D) VOLUME: 5     -           (F) PAGES: 527-534     -           (G) DATE: 1989     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:111:     - Leu Lys Met Glu Trp Ser Ile Gln Ala Thr Th - #r Asn Ala Gln Lys     #               015     - Trp Ala Asn Lys Cys Ile Leu Glu His Ser Se - #r Lys Asp Asp Arg     #               030     - Lys Ile Asn Ile Arg Cys Gly Glu Asn Leu Ty - #r Met Ser Thr Asp     #               045     - Pro Thr Leu Trp Ser Thr Val Ile Gln Ser Tr - #p Tyr Asn Glu Asn     #               060     - Glu Asp Phe Val Tyr Gly Val Gly Ala Lys Pr - #o Asn Ser Ala Val     #               075     - Gly His Tyr Thr Gln Leu Val Trp Tyr Ser Se - #r Phe Lys Ile Gly     #               090     - Cys Gly Ile Ala Tyr Cys Pro Asn Gln Asp As - #n Leu Lys Tyr Phe     #               105     - Tyr Val Cys His Tyr Cys Pro Met Gly Asn As - #n Val Met Lys Lys     #               120     - Ser Thr Pro Tyr Gln Gln Gly Thr Pro Cys Al - #a Ser Cys Pro Asn     #               135     - Asn Cys     - (2) INFORMATION FOR SEQ ID NO:112:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 138     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: human     -     (ix) FEATURE: Testis specific protein; Ta - #ble 17 Row 8     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #M.              Kasahara,     #J.            Gutknecht,                    Brew, K.                    Spurr, N.     #P. N.         Goodfellow,     #mapping of a testis-specific gene with     #   sequence similarity to a sperm coating gl - #ycoprotein     #   gene               (C) JOURNAL: Genomics     -           (D) VOLUME: 5     -           (F) PAGES: 527-534     -           (G) DATE: 1989     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:112:     - Leu Lys Met Glu Trp Ser Arg Glu Val Thr Th - #r Asn Ala Gln Arg     #               015     - Trp Ala Asn Lys Cys Thr Leu Gln His Ser As - #p Pro Glu Asp Arg     #               030     - Lys Thr Ser Thr Arg Cys Gly Glu Asn Leu Ty - #r Met Ser Ser Asp     #               045     - Pro Thr Ser Trp Ser Ser Ala Ile Gln Ser Tr - #p Tyr Asp Glu Ile     #               060     - Leu Asp Phe Val Tyr Gly Val Gly Pro Lys Se - #r Pro Asn Ala Val     #               075     - Val Gly His Tyr Thr Gln Leu Val Trp Tyr Se - #r Thr Tyr Gln Val     #               090     - Gly Cys Gly Ile Ala Tyr Cys Pro Asn Gln As - #p Ser Leu Lys Tyr     #               105     - Tyr Tyr Val Cys Gln Tyr Cys Pro Ala Gly As - #n Asn Met Asn Arg     #               120     - Lys Asn Thr Pro Tyr Gln Gln Gly Thr Pro Cy - #s Ala Gly Cys Pro     #               135     - Asp Asp Cys     - (2) INFORMATION FOR SEQ ID NO:113:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 124     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Dolichovespu - #la maculata (white face hornet)     #Row 9(ix) FEATURE: venom allergen; Table 17     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #S. Y.           Fang, K.                    Vitale, M - #.     #P.            Fehlner,     #P.            King, T.               (B) TITLE: cDNA cloning - # and primary structure of a white-fac     #   hornet venom allergen, antigen 5.     #of the National Academy of Sciences,     #     U.S.A.               (D) VOLUME: 85     -           (F) PAGES: 895-899     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:113:     - Asn Val Leu Val Trp Asn Asp Glu Leu Ala Ly - #s Ile Ala Gln Thr     #               015     - Trp Ala Asn Gln Cys Asp Phe Asn His Asp As - #p Cys Arg Asn Thr     #               030     - Ala Lys Tyr Gln Val Gly Gln Asn Ile Ala Il - #e Ser Ser Thr Thr     #               045     - Ala Thr Gln Phe Asp Arg Pro Ser Lys Leu Il - #e Lys Gln Trp Glu     #               060     - Asp Glu Val Thr Glu Phe Asn Tyr Lys Val Gl - #y Leu Gln Asn Ser     #               075     - Asn Phe Arg Lys Val Gly His Tyr Thr Gln Me - #t Val Trp Gly Lys     #               090     - Thr Lys Glu Ile Gly Cys Gly Ser Ile Lys Ty - #r Ile Glu Asp Asn     #               105     - Trp Tyr Thr His Tyr Leu Val Cys Asn Tyr Gl - #y Pro Gly Gly Asn     #               120     - Asp Phe Asn Gln     - (2) INFORMATION FOR SEQ ID NO:114:     -      (i) SEQUENCE CHARACTERISTICS:               (A) LENGTH: 125     -           (B) TYPE: amino acid     -           (D) TOPOLOGY: linear     -     (ii) MOLECULE TYPE:               (A) DESCRIPTION: protein     -     (vi) ORIGINAL SOURCE:               (A) ORGANISM: Dolichovespu - #la maculata (white face hornet)     #Row 10ix) FEATURE: Venom allergen; Table 17     -      (x) PUBLICATION INFORMATION:               (A) AUTHORS:     #S. Y.           Fang, K.                    Vitale, M - #.     #P.            Fehlner,     #P.            King, T.               (B) TITLE: cDNA cloning - # and primary structure of a white-fac     e     #   hornet venom allergen, antigen 5.     #of the National Academy of Sciences,     #     U.S.A.               (D) VOLUME: 85     -           (F) PAGES: 895-899     -           (G) DATE: 1988     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:114:     - Asn Val Leu Val Trp Asn Asp Glu Leu Ala Ly - #s Ile Ala Gln Thr     #               015     - Trp Ala Asn Gln Cys Ser Phe Gly His Asp Gl - #n Cys Arg Asn Thr     #               030     - Glu Lys Tyr Gln Val Gly Gln Asn Val Ala Il - #e Ala Ser Thr Thr     #               045     - Gly Asn Ser Tyr Ala Thr Met Ser Lys Leu Il - #e Glu Met Trp Glu     #               060     - Asn Glu Val Lys Asp Phe Asn Pro Lys Lys Gl - #y Thr Met Gly Asp     #               075     - Asn Asn Phe Ser Lys Val Gly His Tyr Thr Gl - #n Met Val Trp Gly     #               090     - Lys Thr Lys Glu Ile Gly Cys Gly Ser Val Ly - #s Tyr Ile Glu Asn     #               105     - Asn Trp His Thr His Tyr Leu Val Cys Asn Ty - #r Gly Pro Ala Gly     #               120     - Asn Tyr Met Asp Gln                     125     __________________________________________________________________________ 

What is claimed is:
 1. A method for predicting the folded structure of proteins that comprises obtaining an alignment of the sequences of a set of homologous proteins, using patterns of conservation and variation of the sequence between proteins with clearly defined evolutionary relationships to assign positions in the alignment to the surface of the folded structure, the inside of the folded structure, active site, or parsing segments, assigning secondary structural units by identifying periodicity in said assignments, and assembling said secondary structural units into a globular form using distance constraints imposed by disulfide bridges, active site assignments, and covariation analysis.
 2. A method for predicting the secondary structure of proteins that comprises obtaining an alignment of the sequences of a set of homologous proteins, using patterns of conservation and variation of the sequence between proteins with clearly defined evolutionary relationships to assign positions in the alignment to the surface of the folded structure, the inside of the folded structure, active site, or parsing segments, and assigning secondary structural units by identifying periodicity in said assignments. 